In situ assessment of DNA damage in Branchiura sowerbyi Beddard, 1892 (Oligochaeta: Tubificidae) from the Sava river using comet assay
Аутори
Kracun-Kolarevic, MargaretaKolarević, Stoimir
Atanacković, Ana
Kostić, Jovana
Gacic, Zoran
Paunović, Momir
Vuković-Gačić, Branka
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Aquatic oligochaete, Branchiura sowerby (Beddard, 1892) is a cosmopolitan tubificid species commonly found in organically enriched freshwater environments. These aquatic worms inhabit river and lake sediment where they feed by decomposing organic matter. B. sowerbyi is characterized by limited mobility and could be used as effective indicator organism. Collecting of B. sowerbyi was done monthly during 2014 on the Sava River (sampling site Duboko). Duboko is situated downstream of city Obrenovac (50.000 inhabitants). The site is under the influence of two major pollution sources. One is the Kolubara River (under the influence of various pollution pressures, including wastewaters from town Obrenovac) and the other is thermal power plant "Nikola Tesla" which is situated few kilometres upstream. Suspensions of cells (haemocytes and coelomocytes) were used for the assessment of genotoxicity by comet assay. As reference the value of Comet response obtained from individuals acclimatized in co...ntrolled aquarium conditions was used. For each sampling images of 200 nuclei were analyzed with a fluorescence microscope and analysed using the software Comet Assay IV. During the period of investigation the wider area of investigation was affected by a huge flooding (May 2014) when entire city of Obrenovac was evacuated and consequently there was not wastewater discharges. Therefore, in May and June one source of pollution was excluded. Comparing with reference value, the significant increasing of DNA damage was recorded during examination period, except in May and June. Also, microbiological quality of water was assessed each month. High correlation with faecal indicators indicates that integrity of DNA molecule of B. sowerbyi is affected by urban pollution.
This research has received funding from the European Community's FP7 agreement n° 603629-ENV-2013-6.2.1.-Globaqua
Кључне речи:
Branchiura sowerbyi / Sava River / flooding / faecal pollution / comet assayИзвор:
Globaqua-Cytothreat-Endetech-Scarce Workshop. Pharmaceuticals in wastewaters and surface waters under multistressors situation: Fate, Adverse effects, Risks and Removal Technologies, Barcelona, Spain, 2014, 61-Издавач:
- GLOBAQUA, CYTOTHREAT, ENDETECH, SCARCE Projects
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Kracun-Kolarevic, Margareta AU - Kolarević, Stoimir AU - Atanacković, Ana AU - Kostić, Jovana AU - Gacic, Zoran AU - Paunović, Momir AU - Vuković-Gačić, Branka PY - 2014 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2683 AB - Aquatic oligochaete, Branchiura sowerby (Beddard, 1892) is a cosmopolitan tubificid species commonly found in organically enriched freshwater environments. These aquatic worms inhabit river and lake sediment where they feed by decomposing organic matter. B. sowerbyi is characterized by limited mobility and could be used as effective indicator organism. Collecting of B. sowerbyi was done monthly during 2014 on the Sava River (sampling site Duboko). Duboko is situated downstream of city Obrenovac (50.000 inhabitants). The site is under the influence of two major pollution sources. One is the Kolubara River (under the influence of various pollution pressures, including wastewaters from town Obrenovac) and the other is thermal power plant "Nikola Tesla" which is situated few kilometres upstream. Suspensions of cells (haemocytes and coelomocytes) were used for the assessment of genotoxicity by comet assay. As reference the value of Comet response obtained from individuals acclimatized in controlled aquarium conditions was used. For each sampling images of 200 nuclei were analyzed with a fluorescence microscope and analysed using the software Comet Assay IV. During the period of investigation the wider area of investigation was affected by a huge flooding (May 2014) when entire city of Obrenovac was evacuated and consequently there was not wastewater discharges. Therefore, in May and June one source of pollution was excluded. Comparing with reference value, the significant increasing of DNA damage was recorded during examination period, except in May and June. Also, microbiological quality of water was assessed each month. High correlation with faecal indicators indicates that integrity of DNA molecule of B. sowerbyi is affected by urban pollution. This research has received funding from the European Community's FP7 agreement n° 603629-ENV-2013-6.2.1.-Globaqua PB - GLOBAQUA, CYTOTHREAT, ENDETECH, SCARCE Projects C3 - Globaqua-Cytothreat-Endetech-Scarce Workshop. Pharmaceuticals in wastewaters and surface waters under multistressors situation: Fate, Adverse effects, Risks and Removal Technologies, Barcelona, Spain T1 - In situ assessment of DNA damage in Branchiura sowerbyi Beddard, 1892 (Oligochaeta: Tubificidae) from the Sava river using comet assay SP - 61 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2683 ER -
@conference{ author = "Kracun-Kolarevic, Margareta and Kolarević, Stoimir and Atanacković, Ana and Kostić, Jovana and Gacic, Zoran and Paunović, Momir and Vuković-Gačić, Branka", year = "2014", abstract = "Aquatic oligochaete, Branchiura sowerby (Beddard, 1892) is a cosmopolitan tubificid species commonly found in organically enriched freshwater environments. These aquatic worms inhabit river and lake sediment where they feed by decomposing organic matter. B. sowerbyi is characterized by limited mobility and could be used as effective indicator organism. Collecting of B. sowerbyi was done monthly during 2014 on the Sava River (sampling site Duboko). Duboko is situated downstream of city Obrenovac (50.000 inhabitants). The site is under the influence of two major pollution sources. One is the Kolubara River (under the influence of various pollution pressures, including wastewaters from town Obrenovac) and the other is thermal power plant "Nikola Tesla" which is situated few kilometres upstream. Suspensions of cells (haemocytes and coelomocytes) were used for the assessment of genotoxicity by comet assay. As reference the value of Comet response obtained from individuals acclimatized in controlled aquarium conditions was used. For each sampling images of 200 nuclei were analyzed with a fluorescence microscope and analysed using the software Comet Assay IV. During the period of investigation the wider area of investigation was affected by a huge flooding (May 2014) when entire city of Obrenovac was evacuated and consequently there was not wastewater discharges. Therefore, in May and June one source of pollution was excluded. Comparing with reference value, the significant increasing of DNA damage was recorded during examination period, except in May and June. Also, microbiological quality of water was assessed each month. High correlation with faecal indicators indicates that integrity of DNA molecule of B. sowerbyi is affected by urban pollution. This research has received funding from the European Community's FP7 agreement n° 603629-ENV-2013-6.2.1.-Globaqua", publisher = "GLOBAQUA, CYTOTHREAT, ENDETECH, SCARCE Projects", journal = "Globaqua-Cytothreat-Endetech-Scarce Workshop. Pharmaceuticals in wastewaters and surface waters under multistressors situation: Fate, Adverse effects, Risks and Removal Technologies, Barcelona, Spain", title = "In situ assessment of DNA damage in Branchiura sowerbyi Beddard, 1892 (Oligochaeta: Tubificidae) from the Sava river using comet assay", pages = "61", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2683" }
Kracun-Kolarevic, M., Kolarević, S., Atanacković, A., Kostić, J., Gacic, Z., Paunović, M.,& Vuković-Gačić, B.. (2014). In situ assessment of DNA damage in Branchiura sowerbyi Beddard, 1892 (Oligochaeta: Tubificidae) from the Sava river using comet assay. in Globaqua-Cytothreat-Endetech-Scarce Workshop. Pharmaceuticals in wastewaters and surface waters under multistressors situation: Fate, Adverse effects, Risks and Removal Technologies, Barcelona, Spain GLOBAQUA, CYTOTHREAT, ENDETECH, SCARCE Projects., 61. https://hdl.handle.net/21.15107/rcub_rimsi_2683
Kracun-Kolarevic M, Kolarević S, Atanacković A, Kostić J, Gacic Z, Paunović M, Vuković-Gačić B. In situ assessment of DNA damage in Branchiura sowerbyi Beddard, 1892 (Oligochaeta: Tubificidae) from the Sava river using comet assay. in Globaqua-Cytothreat-Endetech-Scarce Workshop. Pharmaceuticals in wastewaters and surface waters under multistressors situation: Fate, Adverse effects, Risks and Removal Technologies, Barcelona, Spain. 2014;:61. https://hdl.handle.net/21.15107/rcub_rimsi_2683 .
Kracun-Kolarevic, Margareta, Kolarević, Stoimir, Atanacković, Ana, Kostić, Jovana, Gacic, Zoran, Paunović, Momir, Vuković-Gačić, Branka, "In situ assessment of DNA damage in Branchiura sowerbyi Beddard, 1892 (Oligochaeta: Tubificidae) from the Sava river using comet assay" in Globaqua-Cytothreat-Endetech-Scarce Workshop. Pharmaceuticals in wastewaters and surface waters under multistressors situation: Fate, Adverse effects, Risks and Removal Technologies, Barcelona, Spain (2014):61, https://hdl.handle.net/21.15107/rcub_rimsi_2683 .