Dynamic of tributyltin influence on DNA damage in haemocytes and cardiac activity of the Mediterranean mussel Mytilus galloprovincialis
Аутори
Martinovic, RajkoKolarević, Stoimir
Kracun-Kolarevic, Margareta
Kostić, Jovana
Jokanović, Sandra
Gacic, Zoran
Joksimovic, Danijela
Djurovic, Mirko
Vuković-Gačić, Branka
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Adverse effects of antifouling paints containing organotin compound tributylin (TBT) marked this agent as one of the most toxic substances released into the sea; therefore, its usage is banned. Considering the lack of TBT related data in the Boka Kotorska Bay, we decide to apply an ecotoxicological test battery of TBT influence on level of DNA damage in haemocytes and cardiac activity of the Mediterranean mussel Mytilus galloprovincialis L. During the 96 h treatment with 10, 100 and 1000 µg/l of TBT chloride and 50 µg/l of benzo(a)pyrene used as positive control, in static system, haemolymph samples were collected after 24, 48, 72 and 96 h from all aquaria. Results were done in duplicate and indicated on reduced cell viability compared to negative controls. The increase of DNA damage level in haemocytes obtained by comet assay and significant increase of micronucleus frequency after 72h of exposure in the first experiment and after 96 h in the second was occurred. By means of SOS/umuC ...assay, using special strains of Salmonella typhimurium, we did not detect mutagenic potency of TBT. However, dose depended decrease in cell biomass was observed. The heart rate recording was carried out by non-invasive method based on infrared sensor technology. The lowest TBT dose induced 10% increase of heart rate up on the treatment onset, while higher concentrations after the initial rise induced significant decline of cardiac activity. Heart rate baseline value was restored later during the treatment with 10 and 100 µg/l of TBT indicating on ability of mussels to maintain regular cardiac activity despite substantial amount of toxin.
Кључне речи:
tributylin / Mytilus galloprovincialis / cardiac activity / comet assay / micronucleus test / SOS/umuC assayИзвор:
The Central & Eastern Europe Conference on Health and the Environment (CEECHE), Prague, Czech Republic, 2016, 91-Издавач:
- Institute of Experimental Medicine (Prague, Czech Republic) and the University of Kentucky (USA)
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Martinovic, Rajko AU - Kolarević, Stoimir AU - Kracun-Kolarevic, Margareta AU - Kostić, Jovana AU - Jokanović, Sandra AU - Gacic, Zoran AU - Joksimovic, Danijela AU - Djurovic, Mirko AU - Vuković-Gačić, Branka PY - 2016 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2682 AB - Adverse effects of antifouling paints containing organotin compound tributylin (TBT) marked this agent as one of the most toxic substances released into the sea; therefore, its usage is banned. Considering the lack of TBT related data in the Boka Kotorska Bay, we decide to apply an ecotoxicological test battery of TBT influence on level of DNA damage in haemocytes and cardiac activity of the Mediterranean mussel Mytilus galloprovincialis L. During the 96 h treatment with 10, 100 and 1000 µg/l of TBT chloride and 50 µg/l of benzo(a)pyrene used as positive control, in static system, haemolymph samples were collected after 24, 48, 72 and 96 h from all aquaria. Results were done in duplicate and indicated on reduced cell viability compared to negative controls. The increase of DNA damage level in haemocytes obtained by comet assay and significant increase of micronucleus frequency after 72h of exposure in the first experiment and after 96 h in the second was occurred. By means of SOS/umuC assay, using special strains of Salmonella typhimurium, we did not detect mutagenic potency of TBT. However, dose depended decrease in cell biomass was observed. The heart rate recording was carried out by non-invasive method based on infrared sensor technology. The lowest TBT dose induced 10% increase of heart rate up on the treatment onset, while higher concentrations after the initial rise induced significant decline of cardiac activity. Heart rate baseline value was restored later during the treatment with 10 and 100 µg/l of TBT indicating on ability of mussels to maintain regular cardiac activity despite substantial amount of toxin. PB - Institute of Experimental Medicine (Prague, Czech Republic) and the University of Kentucky (USA) C3 - The Central & Eastern Europe Conference on Health and the Environment (CEECHE), Prague, Czech Republic T1 - Dynamic of tributyltin influence on DNA damage in haemocytes and cardiac activity of the Mediterranean mussel Mytilus galloprovincialis SP - 91 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2682 ER -
@conference{ author = "Martinovic, Rajko and Kolarević, Stoimir and Kracun-Kolarevic, Margareta and Kostić, Jovana and Jokanović, Sandra and Gacic, Zoran and Joksimovic, Danijela and Djurovic, Mirko and Vuković-Gačić, Branka", year = "2016", abstract = "Adverse effects of antifouling paints containing organotin compound tributylin (TBT) marked this agent as one of the most toxic substances released into the sea; therefore, its usage is banned. Considering the lack of TBT related data in the Boka Kotorska Bay, we decide to apply an ecotoxicological test battery of TBT influence on level of DNA damage in haemocytes and cardiac activity of the Mediterranean mussel Mytilus galloprovincialis L. During the 96 h treatment with 10, 100 and 1000 µg/l of TBT chloride and 50 µg/l of benzo(a)pyrene used as positive control, in static system, haemolymph samples were collected after 24, 48, 72 and 96 h from all aquaria. Results were done in duplicate and indicated on reduced cell viability compared to negative controls. The increase of DNA damage level in haemocytes obtained by comet assay and significant increase of micronucleus frequency after 72h of exposure in the first experiment and after 96 h in the second was occurred. By means of SOS/umuC assay, using special strains of Salmonella typhimurium, we did not detect mutagenic potency of TBT. However, dose depended decrease in cell biomass was observed. The heart rate recording was carried out by non-invasive method based on infrared sensor technology. The lowest TBT dose induced 10% increase of heart rate up on the treatment onset, while higher concentrations after the initial rise induced significant decline of cardiac activity. Heart rate baseline value was restored later during the treatment with 10 and 100 µg/l of TBT indicating on ability of mussels to maintain regular cardiac activity despite substantial amount of toxin.", publisher = "Institute of Experimental Medicine (Prague, Czech Republic) and the University of Kentucky (USA)", journal = "The Central & Eastern Europe Conference on Health and the Environment (CEECHE), Prague, Czech Republic", title = "Dynamic of tributyltin influence on DNA damage in haemocytes and cardiac activity of the Mediterranean mussel Mytilus galloprovincialis", pages = "91", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2682" }
Martinovic, R., Kolarević, S., Kracun-Kolarevic, M., Kostić, J., Jokanović, S., Gacic, Z., Joksimovic, D., Djurovic, M.,& Vuković-Gačić, B.. (2016). Dynamic of tributyltin influence on DNA damage in haemocytes and cardiac activity of the Mediterranean mussel Mytilus galloprovincialis. in The Central & Eastern Europe Conference on Health and the Environment (CEECHE), Prague, Czech Republic Institute of Experimental Medicine (Prague, Czech Republic) and the University of Kentucky (USA)., 91. https://hdl.handle.net/21.15107/rcub_rimsi_2682
Martinovic R, Kolarević S, Kracun-Kolarevic M, Kostić J, Jokanović S, Gacic Z, Joksimovic D, Djurovic M, Vuković-Gačić B. Dynamic of tributyltin influence on DNA damage in haemocytes and cardiac activity of the Mediterranean mussel Mytilus galloprovincialis. in The Central & Eastern Europe Conference on Health and the Environment (CEECHE), Prague, Czech Republic. 2016;:91. https://hdl.handle.net/21.15107/rcub_rimsi_2682 .
Martinovic, Rajko, Kolarević, Stoimir, Kracun-Kolarevic, Margareta, Kostić, Jovana, Jokanović, Sandra, Gacic, Zoran, Joksimovic, Danijela, Djurovic, Mirko, Vuković-Gačić, Branka, "Dynamic of tributyltin influence on DNA damage in haemocytes and cardiac activity of the Mediterranean mussel Mytilus galloprovincialis" in The Central & Eastern Europe Conference on Health and the Environment (CEECHE), Prague, Czech Republic (2016):91, https://hdl.handle.net/21.15107/rcub_rimsi_2682 .