Assesment of genotoxic pollution of the Danube River with comet assay in different tissues of common bream (Abramis brama L. 1758)
Autori
Aborgiba, MustafaElbahi, Samia
Kolarević, Stoimir
Kostić, Jovana
Kracun-Kolarevic, Margareta
Knežević-Vukčević, Jelena
Lenhardt, Mirjana
Gacic, Zoran
Vuković-Gačić, Branka
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The aim of this study was to assess the genotoxic potential of Danube River at the site Višnjica (downstream Belgrade) on monthly basis over year 2014 in tissues of common bream (A. brama). The level of DNA damage in the three tissues, blood, gills and liver was measured by comet assay as one of the major tools for assessing pollution related genotoxicity in aquatic organisms. Images of scored nucleoids were analyzed using software (Comet Assay IV). Significant differences in the level of DNA damage were observed in different tissues. For all three tissues, levels of DNA damage were higher comparing to data obtained in our previous research on Cyprinidae family from unpolluted site Special Nature Reserve “Uvac”. These results indicate the presence of genotoxic pollution at the site Višnjica. The samples of tissues were also collected for histopatology and heavy metals analyses which will provide further insight in the origin and fate of the detected genotoxic influence. Physical and ch...emical parameters and microbiological quality of water assessed each month are in correlation with comet assay results.
Ključne reči:
comet assay / Abramis brama / genotoxicity / the Danube RiverIzvor:
V Congress of the Serbian Genetic Society, 2014, 131-Izdavač:
- Serbian Genetic Society, Belgrade, Serbia
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - CONF AU - Aborgiba, Mustafa AU - Elbahi, Samia AU - Kolarević, Stoimir AU - Kostić, Jovana AU - Kracun-Kolarevic, Margareta AU - Knežević-Vukčević, Jelena AU - Lenhardt, Mirjana AU - Gacic, Zoran AU - Vuković-Gačić, Branka PY - 2014 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2493 AB - The aim of this study was to assess the genotoxic potential of Danube River at the site Višnjica (downstream Belgrade) on monthly basis over year 2014 in tissues of common bream (A. brama). The level of DNA damage in the three tissues, blood, gills and liver was measured by comet assay as one of the major tools for assessing pollution related genotoxicity in aquatic organisms. Images of scored nucleoids were analyzed using software (Comet Assay IV). Significant differences in the level of DNA damage were observed in different tissues. For all three tissues, levels of DNA damage were higher comparing to data obtained in our previous research on Cyprinidae family from unpolluted site Special Nature Reserve “Uvac”. These results indicate the presence of genotoxic pollution at the site Višnjica. The samples of tissues were also collected for histopatology and heavy metals analyses which will provide further insight in the origin and fate of the detected genotoxic influence. Physical and chemical parameters and microbiological quality of water assessed each month are in correlation with comet assay results. PB - Serbian Genetic Society, Belgrade, Serbia C3 - V Congress of the Serbian Genetic Society T1 - Assesment of genotoxic pollution of the Danube River with comet assay in different tissues of common bream (Abramis brama L. 1758) SP - 131 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2493 ER -
@conference{ author = "Aborgiba, Mustafa and Elbahi, Samia and Kolarević, Stoimir and Kostić, Jovana and Kracun-Kolarevic, Margareta and Knežević-Vukčević, Jelena and Lenhardt, Mirjana and Gacic, Zoran and Vuković-Gačić, Branka", year = "2014", abstract = "The aim of this study was to assess the genotoxic potential of Danube River at the site Višnjica (downstream Belgrade) on monthly basis over year 2014 in tissues of common bream (A. brama). The level of DNA damage in the three tissues, blood, gills and liver was measured by comet assay as one of the major tools for assessing pollution related genotoxicity in aquatic organisms. Images of scored nucleoids were analyzed using software (Comet Assay IV). Significant differences in the level of DNA damage were observed in different tissues. For all three tissues, levels of DNA damage were higher comparing to data obtained in our previous research on Cyprinidae family from unpolluted site Special Nature Reserve “Uvac”. These results indicate the presence of genotoxic pollution at the site Višnjica. The samples of tissues were also collected for histopatology and heavy metals analyses which will provide further insight in the origin and fate of the detected genotoxic influence. Physical and chemical parameters and microbiological quality of water assessed each month are in correlation with comet assay results.", publisher = "Serbian Genetic Society, Belgrade, Serbia", journal = "V Congress of the Serbian Genetic Society", title = "Assesment of genotoxic pollution of the Danube River with comet assay in different tissues of common bream (Abramis brama L. 1758)", pages = "131", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2493" }
Aborgiba, M., Elbahi, S., Kolarević, S., Kostić, J., Kracun-Kolarevic, M., Knežević-Vukčević, J., Lenhardt, M., Gacic, Z.,& Vuković-Gačić, B.. (2014). Assesment of genotoxic pollution of the Danube River with comet assay in different tissues of common bream (Abramis brama L. 1758). in V Congress of the Serbian Genetic Society Serbian Genetic Society, Belgrade, Serbia., 131. https://hdl.handle.net/21.15107/rcub_rimsi_2493
Aborgiba M, Elbahi S, Kolarević S, Kostić J, Kracun-Kolarevic M, Knežević-Vukčević J, Lenhardt M, Gacic Z, Vuković-Gačić B. Assesment of genotoxic pollution of the Danube River with comet assay in different tissues of common bream (Abramis brama L. 1758). in V Congress of the Serbian Genetic Society. 2014;:131. https://hdl.handle.net/21.15107/rcub_rimsi_2493 .
Aborgiba, Mustafa, Elbahi, Samia, Kolarević, Stoimir, Kostić, Jovana, Kracun-Kolarevic, Margareta, Knežević-Vukčević, Jelena, Lenhardt, Mirjana, Gacic, Zoran, Vuković-Gačić, Branka, "Assesment of genotoxic pollution of the Danube River with comet assay in different tissues of common bream (Abramis brama L. 1758)" in V Congress of the Serbian Genetic Society (2014):131, https://hdl.handle.net/21.15107/rcub_rimsi_2493 .