Comparative analysis of hydroxyl radical production in fresh and desiccated fronds of Asplenium ceterach L. examined by fluorescence microscopy
Само за регистроване кориснике
2013
Аутори
Živković, S.Mišić, D.
Savić, A.
Jovanović, K.
Popović Bijelić, A. D.
Mojović, M. D.
Milenković, Ivana
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Group of higher plants, known as desiccation tolerant or resurrection plants, possess uniquely effective mechanism to withstand extreme dehydration and rapid rehydration of vegetative tissues without cell damage. Resurrection plants survive the loss of most of their tissues water content until a quiescent stage is achieved. Upon watering, the plants revive and are restored to their former state within 24h. In order to cope with desiccation, resurrection plants have to overcome a number of stresses, among them the most critical being oxidative stress. In our previous investigations, we have shown that during the rehydration process in A. ceterach, •OH radicals are produced.
In vivo productions of free radicals remain unexplained and difficult for examination. By application of specific fluorescence probe for detection of hydroxyl radical APF (3’-(p-aminophenyl) fluorescein) it is possible to get insight into processes occurring in fronds during the dehydration process. In reaction of ...hydroxyl radical with APF, fluorescent product emerges. Examination under fluorescence microscope and image capturing in time intervals provides determination of hydroxyl radical production in different plant tissues. Through image analysis based on multivariate techniques, it is possible to simultaneously distinguish plant tissues according to its morphological and physiological properties.
Кључне речи:
Asplenium ceterach / fluorescence / hydroxyl / microscopy / radicalИзвор:
1st International Conference on Plant Biology (20th Symposium of the Serbian Plant Physiology Society), 2013Издавач:
- Serbian Plant Physiology Society
Финансирање / пројекти:
- Синтеза, процесирање и карактеризација наноструктурних материјала за примену у области енергије, механичког инжењерства, заштите животне стредине и биомедицине (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45012)
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Živković, S. AU - Mišić, D. AU - Savić, A. AU - Jovanović, K. AU - Popović Bijelić, A. D. AU - Mojović, M. D. AU - Milenković, Ivana PY - 2013 UR - https://enauka.gov.rs/handle/123456789/229381 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2295 AB - Group of higher plants, known as desiccation tolerant or resurrection plants, possess uniquely effective mechanism to withstand extreme dehydration and rapid rehydration of vegetative tissues without cell damage. Resurrection plants survive the loss of most of their tissues water content until a quiescent stage is achieved. Upon watering, the plants revive and are restored to their former state within 24h. In order to cope with desiccation, resurrection plants have to overcome a number of stresses, among them the most critical being oxidative stress. In our previous investigations, we have shown that during the rehydration process in A. ceterach, •OH radicals are produced. In vivo productions of free radicals remain unexplained and difficult for examination. By application of specific fluorescence probe for detection of hydroxyl radical APF (3’-(p-aminophenyl) fluorescein) it is possible to get insight into processes occurring in fronds during the dehydration process. In reaction of hydroxyl radical with APF, fluorescent product emerges. Examination under fluorescence microscope and image capturing in time intervals provides determination of hydroxyl radical production in different plant tissues. Through image analysis based on multivariate techniques, it is possible to simultaneously distinguish plant tissues according to its morphological and physiological properties. PB - Serbian Plant Physiology Society C3 - 1st International Conference on Plant Biology (20th Symposium of the Serbian Plant Physiology Society) T1 - Comparative analysis of hydroxyl radical production in fresh and desiccated fronds of Asplenium ceterach L. examined by fluorescence microscopy UR - https://hdl.handle.net/21.15107/rcub_rimsi_2295 ER -
@conference{ author = "Živković, S. and Mišić, D. and Savić, A. and Jovanović, K. and Popović Bijelić, A. D. and Mojović, M. D. and Milenković, Ivana", year = "2013", abstract = "Group of higher plants, known as desiccation tolerant or resurrection plants, possess uniquely effective mechanism to withstand extreme dehydration and rapid rehydration of vegetative tissues without cell damage. Resurrection plants survive the loss of most of their tissues water content until a quiescent stage is achieved. Upon watering, the plants revive and are restored to their former state within 24h. In order to cope with desiccation, resurrection plants have to overcome a number of stresses, among them the most critical being oxidative stress. In our previous investigations, we have shown that during the rehydration process in A. ceterach, •OH radicals are produced. In vivo productions of free radicals remain unexplained and difficult for examination. By application of specific fluorescence probe for detection of hydroxyl radical APF (3’-(p-aminophenyl) fluorescein) it is possible to get insight into processes occurring in fronds during the dehydration process. In reaction of hydroxyl radical with APF, fluorescent product emerges. Examination under fluorescence microscope and image capturing in time intervals provides determination of hydroxyl radical production in different plant tissues. Through image analysis based on multivariate techniques, it is possible to simultaneously distinguish plant tissues according to its morphological and physiological properties.", publisher = "Serbian Plant Physiology Society", journal = "1st International Conference on Plant Biology (20th Symposium of the Serbian Plant Physiology Society)", title = "Comparative analysis of hydroxyl radical production in fresh and desiccated fronds of Asplenium ceterach L. examined by fluorescence microscopy", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2295" }
Živković, S., Mišić, D., Savić, A., Jovanović, K., Popović Bijelić, A. D., Mojović, M. D.,& Milenković, I.. (2013). Comparative analysis of hydroxyl radical production in fresh and desiccated fronds of Asplenium ceterach L. examined by fluorescence microscopy. in 1st International Conference on Plant Biology (20th Symposium of the Serbian Plant Physiology Society) Serbian Plant Physiology Society.. https://hdl.handle.net/21.15107/rcub_rimsi_2295
Živković S, Mišić D, Savić A, Jovanović K, Popović Bijelić AD, Mojović MD, Milenković I. Comparative analysis of hydroxyl radical production in fresh and desiccated fronds of Asplenium ceterach L. examined by fluorescence microscopy. in 1st International Conference on Plant Biology (20th Symposium of the Serbian Plant Physiology Society). 2013;. https://hdl.handle.net/21.15107/rcub_rimsi_2295 .
Živković, S., Mišić, D., Savić, A., Jovanović, K., Popović Bijelić, A. D., Mojović, M. D., Milenković, Ivana, "Comparative analysis of hydroxyl radical production in fresh and desiccated fronds of Asplenium ceterach L. examined by fluorescence microscopy" in 1st International Conference on Plant Biology (20th Symposium of the Serbian Plant Physiology Society) (2013), https://hdl.handle.net/21.15107/rcub_rimsi_2295 .