Evaluation of biological activity of selected tert-butylquinone derivatives as potential antitumor drugs
Само за регистроване кориснике
2019
Аутори
Vuković-Gačić, BrankaĐorđević Aleksić, Jelena
Kolarević, Stoimir
Jovanović Marić, Jovana
Novaković, Irena
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The biological activity of selected of 2-tert-butyl-1.4-benzoquinone (TBQ) and its derivatives: 2-tert-buty|-5-(isopropylthio)-1.4-benzoquinone, 2-tert-butyl-5-(propylthio)-1.4-benzoquinone, 2-tert-butyl-5,6-(ethylendithio)-1.4-benzoquinone, 2-tert-butyl-5-(phenylthio)-1.4-benzoquinone and 2-tert-butyl-6-(phenyithio)-1.4-benzoquinone was examined by their antibacterial, toxic, cytotoxic and genotoxic potential. The antimicrobial potential of derivatives was investigated by the broth microdilution method establishing minimal inhibitory concentration for 4 gram-negative and 4 gram-positive bacteria. Toxicity has been tested by 24 h treatment of Artemia nauplii by ARC test. Cytotoxicity was assessed in 24 h treatment in three different cell lines with MTT and MTS assay: human fetal lung cell line (MRC-5), human cancer cell line - melanoma (HS 294T) and human liver cancer cell line (HepG2). Genotoxicity was evaluated after 24 h treatment by comet assay in MRC-5 and HepG2 cell lines. In gen...eral, result indicated that modification of TBQ enhanced its activity. All substances exhibited antibacterial potential but only against gram-positive bacteria whereas 2-tert-butyl-5,6-(ethylendithio)-1.4-benzoquinone showed the strongest antibacterial activity. Results of ARC test indicated that synthesized derivatives (with exception of 2-tert-butyl-6-(phenylthio)-1.4-benzoquinone) lose their toxic potential in comparison with parent compound TBQ. Derivatives exhibited stronger cytotoxic activity a all three cell lines in comparison with TBQ, agan 2-tert-butyl-5,6-(ethylendithio)-1.4-benzoquinone. Results of the comet assay derivatives showed the strongest activity also have indicated stronger genotoxic, potential in comparison with TBQ itself indicating that DNA damage could be one of the pathmay in cytotoxicity induction.
Кључне речи:
TBQ / toxicity / citotoxicity / Artemia salina / cancer cellsИзвор:
47th Annual Meeting of EEMGS, Rennes, France, 19-23 May 2019, 2019, 72-Издавач:
- EEMGS
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Vuković-Gačić, Branka AU - Đorđević Aleksić, Jelena AU - Kolarević, Stoimir AU - Jovanović Marić, Jovana AU - Novaković, Irena PY - 2019 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2151 AB - The biological activity of selected of 2-tert-butyl-1.4-benzoquinone (TBQ) and its derivatives: 2-tert-buty|-5-(isopropylthio)-1.4-benzoquinone, 2-tert-butyl-5-(propylthio)-1.4-benzoquinone, 2-tert-butyl-5,6-(ethylendithio)-1.4-benzoquinone, 2-tert-butyl-5-(phenylthio)-1.4-benzoquinone and 2-tert-butyl-6-(phenyithio)-1.4-benzoquinone was examined by their antibacterial, toxic, cytotoxic and genotoxic potential. The antimicrobial potential of derivatives was investigated by the broth microdilution method establishing minimal inhibitory concentration for 4 gram-negative and 4 gram-positive bacteria. Toxicity has been tested by 24 h treatment of Artemia nauplii by ARC test. Cytotoxicity was assessed in 24 h treatment in three different cell lines with MTT and MTS assay: human fetal lung cell line (MRC-5), human cancer cell line - melanoma (HS 294T) and human liver cancer cell line (HepG2). Genotoxicity was evaluated after 24 h treatment by comet assay in MRC-5 and HepG2 cell lines. In general, result indicated that modification of TBQ enhanced its activity. All substances exhibited antibacterial potential but only against gram-positive bacteria whereas 2-tert-butyl-5,6-(ethylendithio)-1.4-benzoquinone showed the strongest antibacterial activity. Results of ARC test indicated that synthesized derivatives (with exception of 2-tert-butyl-6-(phenylthio)-1.4-benzoquinone) lose their toxic potential in comparison with parent compound TBQ. Derivatives exhibited stronger cytotoxic activity a all three cell lines in comparison with TBQ, agan 2-tert-butyl-5,6-(ethylendithio)-1.4-benzoquinone. Results of the comet assay derivatives showed the strongest activity also have indicated stronger genotoxic, potential in comparison with TBQ itself indicating that DNA damage could be one of the pathmay in cytotoxicity induction. PB - EEMGS C3 - 47th Annual Meeting of EEMGS, Rennes, France, 19-23 May 2019 T1 - Evaluation of biological activity of selected tert-butylquinone derivatives as potential antitumor drugs SP - 72 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2151 ER -
@conference{ author = "Vuković-Gačić, Branka and Đorđević Aleksić, Jelena and Kolarević, Stoimir and Jovanović Marić, Jovana and Novaković, Irena", year = "2019", abstract = "The biological activity of selected of 2-tert-butyl-1.4-benzoquinone (TBQ) and its derivatives: 2-tert-buty|-5-(isopropylthio)-1.4-benzoquinone, 2-tert-butyl-5-(propylthio)-1.4-benzoquinone, 2-tert-butyl-5,6-(ethylendithio)-1.4-benzoquinone, 2-tert-butyl-5-(phenylthio)-1.4-benzoquinone and 2-tert-butyl-6-(phenyithio)-1.4-benzoquinone was examined by their antibacterial, toxic, cytotoxic and genotoxic potential. The antimicrobial potential of derivatives was investigated by the broth microdilution method establishing minimal inhibitory concentration for 4 gram-negative and 4 gram-positive bacteria. Toxicity has been tested by 24 h treatment of Artemia nauplii by ARC test. Cytotoxicity was assessed in 24 h treatment in three different cell lines with MTT and MTS assay: human fetal lung cell line (MRC-5), human cancer cell line - melanoma (HS 294T) and human liver cancer cell line (HepG2). Genotoxicity was evaluated after 24 h treatment by comet assay in MRC-5 and HepG2 cell lines. In general, result indicated that modification of TBQ enhanced its activity. All substances exhibited antibacterial potential but only against gram-positive bacteria whereas 2-tert-butyl-5,6-(ethylendithio)-1.4-benzoquinone showed the strongest antibacterial activity. Results of ARC test indicated that synthesized derivatives (with exception of 2-tert-butyl-6-(phenylthio)-1.4-benzoquinone) lose their toxic potential in comparison with parent compound TBQ. Derivatives exhibited stronger cytotoxic activity a all three cell lines in comparison with TBQ, agan 2-tert-butyl-5,6-(ethylendithio)-1.4-benzoquinone. Results of the comet assay derivatives showed the strongest activity also have indicated stronger genotoxic, potential in comparison with TBQ itself indicating that DNA damage could be one of the pathmay in cytotoxicity induction.", publisher = "EEMGS", journal = "47th Annual Meeting of EEMGS, Rennes, France, 19-23 May 2019", title = "Evaluation of biological activity of selected tert-butylquinone derivatives as potential antitumor drugs", pages = "72", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2151" }
Vuković-Gačić, B., Đorđević Aleksić, J., Kolarević, S., Jovanović Marić, J.,& Novaković, I.. (2019). Evaluation of biological activity of selected tert-butylquinone derivatives as potential antitumor drugs. in 47th Annual Meeting of EEMGS, Rennes, France, 19-23 May 2019 EEMGS., 72. https://hdl.handle.net/21.15107/rcub_rimsi_2151
Vuković-Gačić B, Đorđević Aleksić J, Kolarević S, Jovanović Marić J, Novaković I. Evaluation of biological activity of selected tert-butylquinone derivatives as potential antitumor drugs. in 47th Annual Meeting of EEMGS, Rennes, France, 19-23 May 2019. 2019;:72. https://hdl.handle.net/21.15107/rcub_rimsi_2151 .
Vuković-Gačić, Branka, Đorđević Aleksić, Jelena, Kolarević, Stoimir, Jovanović Marić, Jovana, Novaković, Irena, "Evaluation of biological activity of selected tert-butylquinone derivatives as potential antitumor drugs" in 47th Annual Meeting of EEMGS, Rennes, France, 19-23 May 2019 (2019):72, https://hdl.handle.net/21.15107/rcub_rimsi_2151 .