Duration of priming with silicon modulates antioxidative response of wheat to salinity stress

Abstract

Priming with silicon (Si) may increase plant resistance to biotic and abiotic stresses, in particular in conjunction with its subsequent application. Yet, the very effect of the duration of priming with Si is less understood. Here, we investigated the effect of the duration of priming with Si on components of the antioxidative response of wheat exposed to a gradient of salinity stress. After priming with 1.5 mM Si(OH)4 (0, 1, and 3 days), wheat seedlings were exposed to different NaCl levels (0, 30, and 60 mM) without (-Si) or with (+Si) supply of 1.5 mM Si(OH)4. The activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), and the concentration of malondialdehyde (MDA) were measured in shoots and roots after 1 and 5 days of NaCl treatments. Interaction of priming duration and addition of Si on antioxidative variables were analyzed using general regression model. Overall, priming had no influence on -Si plants under salt stress. On the day 1 of NaCl exposure, priming duration did not affect SOD and APX activities neither in roots nor in shoots. However, on the day 5 of NaCl exposure longer priming with Si significantly increased the activities of APX and SOD in both roots and shoots. The activity of CAT showed no response to priming with Si and subsequent Si supply in both plant organs irrespectively of the duration of NaCl exposure. Interestingly, in both organs the concentrations of MDA as a proxy for oxidative damage of plant membranes were very clearly and consistently lower after 3 days of priming with Si (compared to 1 day or no priming) during the whole period of NaCl exposure. This study demonstrated that longer priming with Si can enhance the ameliorative effect of Si supply on the antioxidative response of wheat plants to a gradient of salinity stress.