Immobilization of horse radish peroxidase on different macroporous glycydil methacrylates for wastewater treatment

Abstract

Removal of phenolic compounds from wastewaters has been investigated by many researchers and it has been shown that horse radish peroxidase can react with aqueous phenolic compound in the presence of hydrogen peroxide and form non-soluble materials that could be easily removed from the aqueous phase. Main disadvantage of this process is enzyme inactivation. In order to increase stability of enzyme and yield of biocatalytic reaction peroxidases were immobilized on different materials. The aim of this study was to test different macroporous glycidyl methacrylates as carriers for enzyme immobilization. Immobilization was performed using carriers with different pore sizes (40, 50, 120 and 200 nm) and glutaraldehyde as activation method. All immobilized enzymes were characterized using pyrogallol and H2O2 as substrates (13 mM and 10 mM, respectively), and specific activity and immobilization yield were determined. Our data demonstrate that the best carrier for immobilization of horse radish peroxidase was SGE EDA 20/12 with pore size of 120 nm.