OPTIMIZATION OF HORSERADISH PEROXIDASE ENCAPSULATION WITHIN TYRAMINE-ALGINATE FOR PHENOL REMOVAL
Autori
Pantić, NevenaPopović, Nikolina
Prokopijević, Miloš
Spasojević, Dragica
Prodanović, Radivoje
Đikanović, Daniela
Prodanović, Olivera
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Phenolic compounds are one of the most common pollutants in aqueous systems, so their removal
from water is of major interest. Among biocatalysts used for phenol removal, horseradish peroxidase
is the most investigated for this purpose. Enzyme inactivation is a major problem which could be
successfully overcome by immobilization of the enzyme onto different polymers. Tyramine-alginate
micro-beads were tested for the immobilization of horseradish peroxidase. Different concentrations of
tyramine-alginate were used and their influence on specific activity of the enzyme was tested.
Increasing concentration of oxidized alginate results in increase of specific activity. Immobilized HRP
was tested for phenol removal in a batch reactor. Presented results were obtained with HRP
immobilized within 10 mol% tyramine-alginate micro-beads. These biocatalysts can be used up to
three cycles.
Ključne reči:
horseradish peroxidase / immobilization / phenol removal / tyramine / alginateIzvor:
27th International Conference Ecological Truth and Environmental Research, 2019, 220-223-Izdavač:
- University of Belgrade, Technical Faculty in Bor
Finansiranje / projekti:
- Ispitivanja odnosa struktura-funkcija u ćelijskom zidu biljaka i izmene strukture zida enzimskim inženjeringom (RS-MESTD-Basic Research (BR or ON)-173017)
- Alergeni, antitela, enzimi i mali fiziološki značajni molekuli: dizajn, struktura, funkcija i značaj (RS-MESTD-Basic Research (BR or ON)-172049)
- Primena biomedicinskog inženjeringa u pretkliničkoj i kliničkoj praksi (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41007)
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - CONF AU - Pantić, Nevena AU - Popović, Nikolina AU - Prokopijević, Miloš AU - Spasojević, Dragica AU - Prodanović, Radivoje AU - Đikanović, Daniela AU - Prodanović, Olivera PY - 2019 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1656 AB - Phenolic compounds are one of the most common pollutants in aqueous systems, so their removal from water is of major interest. Among biocatalysts used for phenol removal, horseradish peroxidase is the most investigated for this purpose. Enzyme inactivation is a major problem which could be successfully overcome by immobilization of the enzyme onto different polymers. Tyramine-alginate micro-beads were tested for the immobilization of horseradish peroxidase. Different concentrations of tyramine-alginate were used and their influence on specific activity of the enzyme was tested. Increasing concentration of oxidized alginate results in increase of specific activity. Immobilized HRP was tested for phenol removal in a batch reactor. Presented results were obtained with HRP immobilized within 10 mol% tyramine-alginate micro-beads. These biocatalysts can be used up to three cycles. PB - University of Belgrade, Technical Faculty in Bor C3 - 27th International Conference Ecological Truth and Environmental Research T1 - OPTIMIZATION OF HORSERADISH PEROXIDASE ENCAPSULATION WITHIN TYRAMINE-ALGINATE FOR PHENOL REMOVAL SP - 220-223 UR - https://hdl.handle.net/21.15107/rcub_rimsi_1656 ER -
@conference{ author = "Pantić, Nevena and Popović, Nikolina and Prokopijević, Miloš and Spasojević, Dragica and Prodanović, Radivoje and Đikanović, Daniela and Prodanović, Olivera", year = "2019", abstract = "Phenolic compounds are one of the most common pollutants in aqueous systems, so their removal from water is of major interest. Among biocatalysts used for phenol removal, horseradish peroxidase is the most investigated for this purpose. Enzyme inactivation is a major problem which could be successfully overcome by immobilization of the enzyme onto different polymers. Tyramine-alginate micro-beads were tested for the immobilization of horseradish peroxidase. Different concentrations of tyramine-alginate were used and their influence on specific activity of the enzyme was tested. Increasing concentration of oxidized alginate results in increase of specific activity. Immobilized HRP was tested for phenol removal in a batch reactor. Presented results were obtained with HRP immobilized within 10 mol% tyramine-alginate micro-beads. These biocatalysts can be used up to three cycles.", publisher = "University of Belgrade, Technical Faculty in Bor", journal = "27th International Conference Ecological Truth and Environmental Research", title = "OPTIMIZATION OF HORSERADISH PEROXIDASE ENCAPSULATION WITHIN TYRAMINE-ALGINATE FOR PHENOL REMOVAL", pages = "220-223", url = "https://hdl.handle.net/21.15107/rcub_rimsi_1656" }
Pantić, N., Popović, N., Prokopijević, M., Spasojević, D., Prodanović, R., Đikanović, D.,& Prodanović, O.. (2019). OPTIMIZATION OF HORSERADISH PEROXIDASE ENCAPSULATION WITHIN TYRAMINE-ALGINATE FOR PHENOL REMOVAL. in 27th International Conference Ecological Truth and Environmental Research University of Belgrade, Technical Faculty in Bor., 220-223. https://hdl.handle.net/21.15107/rcub_rimsi_1656
Pantić N, Popović N, Prokopijević M, Spasojević D, Prodanović R, Đikanović D, Prodanović O. OPTIMIZATION OF HORSERADISH PEROXIDASE ENCAPSULATION WITHIN TYRAMINE-ALGINATE FOR PHENOL REMOVAL. in 27th International Conference Ecological Truth and Environmental Research. 2019;:220-223. https://hdl.handle.net/21.15107/rcub_rimsi_1656 .
Pantić, Nevena, Popović, Nikolina, Prokopijević, Miloš, Spasojević, Dragica, Prodanović, Radivoje, Đikanović, Daniela, Prodanović, Olivera, "OPTIMIZATION OF HORSERADISH PEROXIDASE ENCAPSULATION WITHIN TYRAMINE-ALGINATE FOR PHENOL REMOVAL" in 27th International Conference Ecological Truth and Environmental Research (2019):220-223, https://hdl.handle.net/21.15107/rcub_rimsi_1656 .