OPTIMIZATION OF HORSERADISH PEROXIDASE ENCAPSULATION WITHIN TYRAMINE-ALGINATE FOR PHENOL REMOVAL
Аутори
Pantić, NevenaPopović, Nikolina
Prokopijević, Miloš
Spasojević, Dragica
Prodanović, Radivoje
Đikanović, Daniela
Prodanović, Olivera
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Phenolic compounds are one of the most common pollutants in aqueous systems, so their removal
from water is of major interest. Among biocatalysts used for phenol removal, horseradish peroxidase
is the most investigated for this purpose. Enzyme inactivation is a major problem which could be
successfully overcome by immobilization of the enzyme onto different polymers. Tyramine-alginate
micro-beads were tested for the immobilization of horseradish peroxidase. Different concentrations of
tyramine-alginate were used and their influence on specific activity of the enzyme was tested.
Increasing concentration of oxidized alginate results in increase of specific activity. Immobilized HRP
was tested for phenol removal in a batch reactor. Presented results were obtained with HRP
immobilized within 10 mol% tyramine-alginate micro-beads. These biocatalysts can be used up to
three cycles.
Кључне речи:
horseradish peroxidase / immobilization / phenol removal / tyramine / alginateИзвор:
27th International Conference Ecological Truth and Environmental Research, 2019, 220-223-Издавач:
- University of Belgrade, Technical Faculty in Bor
Финансирање / пројекти:
- Испитивања односа структура-функција у ћелијском зиду биљака и измене структуре зида ензимским инжењерингом (RS-MESTD-Basic Research (BR or ON)-173017)
- Алергени, антитела, ензими и мали физиолошки значајни молекули: дизајн, структура, функција и значај (RS-MESTD-Basic Research (BR or ON)-172049)
- Примена биомедицинског инжењеринга у претклиничкој и клиничкој пракси (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41007)
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Pantić, Nevena AU - Popović, Nikolina AU - Prokopijević, Miloš AU - Spasojević, Dragica AU - Prodanović, Radivoje AU - Đikanović, Daniela AU - Prodanović, Olivera PY - 2019 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1656 AB - Phenolic compounds are one of the most common pollutants in aqueous systems, so their removal from water is of major interest. Among biocatalysts used for phenol removal, horseradish peroxidase is the most investigated for this purpose. Enzyme inactivation is a major problem which could be successfully overcome by immobilization of the enzyme onto different polymers. Tyramine-alginate micro-beads were tested for the immobilization of horseradish peroxidase. Different concentrations of tyramine-alginate were used and their influence on specific activity of the enzyme was tested. Increasing concentration of oxidized alginate results in increase of specific activity. Immobilized HRP was tested for phenol removal in a batch reactor. Presented results were obtained with HRP immobilized within 10 mol% tyramine-alginate micro-beads. These biocatalysts can be used up to three cycles. PB - University of Belgrade, Technical Faculty in Bor C3 - 27th International Conference Ecological Truth and Environmental Research T1 - OPTIMIZATION OF HORSERADISH PEROXIDASE ENCAPSULATION WITHIN TYRAMINE-ALGINATE FOR PHENOL REMOVAL SP - 220-223 UR - https://hdl.handle.net/21.15107/rcub_rimsi_1656 ER -
@conference{ author = "Pantić, Nevena and Popović, Nikolina and Prokopijević, Miloš and Spasojević, Dragica and Prodanović, Radivoje and Đikanović, Daniela and Prodanović, Olivera", year = "2019", abstract = "Phenolic compounds are one of the most common pollutants in aqueous systems, so their removal from water is of major interest. Among biocatalysts used for phenol removal, horseradish peroxidase is the most investigated for this purpose. Enzyme inactivation is a major problem which could be successfully overcome by immobilization of the enzyme onto different polymers. Tyramine-alginate micro-beads were tested for the immobilization of horseradish peroxidase. Different concentrations of tyramine-alginate were used and their influence on specific activity of the enzyme was tested. Increasing concentration of oxidized alginate results in increase of specific activity. Immobilized HRP was tested for phenol removal in a batch reactor. Presented results were obtained with HRP immobilized within 10 mol% tyramine-alginate micro-beads. These biocatalysts can be used up to three cycles.", publisher = "University of Belgrade, Technical Faculty in Bor", journal = "27th International Conference Ecological Truth and Environmental Research", title = "OPTIMIZATION OF HORSERADISH PEROXIDASE ENCAPSULATION WITHIN TYRAMINE-ALGINATE FOR PHENOL REMOVAL", pages = "220-223", url = "https://hdl.handle.net/21.15107/rcub_rimsi_1656" }
Pantić, N., Popović, N., Prokopijević, M., Spasojević, D., Prodanović, R., Đikanović, D.,& Prodanović, O.. (2019). OPTIMIZATION OF HORSERADISH PEROXIDASE ENCAPSULATION WITHIN TYRAMINE-ALGINATE FOR PHENOL REMOVAL. in 27th International Conference Ecological Truth and Environmental Research University of Belgrade, Technical Faculty in Bor., 220-223. https://hdl.handle.net/21.15107/rcub_rimsi_1656
Pantić N, Popović N, Prokopijević M, Spasojević D, Prodanović R, Đikanović D, Prodanović O. OPTIMIZATION OF HORSERADISH PEROXIDASE ENCAPSULATION WITHIN TYRAMINE-ALGINATE FOR PHENOL REMOVAL. in 27th International Conference Ecological Truth and Environmental Research. 2019;:220-223. https://hdl.handle.net/21.15107/rcub_rimsi_1656 .
Pantić, Nevena, Popović, Nikolina, Prokopijević, Miloš, Spasojević, Dragica, Prodanović, Radivoje, Đikanović, Daniela, Prodanović, Olivera, "OPTIMIZATION OF HORSERADISH PEROXIDASE ENCAPSULATION WITHIN TYRAMINE-ALGINATE FOR PHENOL REMOVAL" in 27th International Conference Ecological Truth and Environmental Research (2019):220-223, https://hdl.handle.net/21.15107/rcub_rimsi_1656 .