A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species
2021
Аутори
Pavlović, Mariana OaldeKolarević, Stoimir
Đorđević Aleksić, Jelena
Jovanović-Marić, Jovana
Lunic, Tanja
Mandic, Marija
Kracun-Kolarević, Margareta
Živković, Jelena
Alimpic-Aradski, Ana
Marin, Petar D.
Savikin, Katarina
Vukovic-Gacic, Branka
Bozić-Nedeljković, Biljana
Duletic-Lausević, Sonja
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of Melissa officinalis, Mentha x piperita, Ocimum basilicum, Rosmarinus officinalis, Salvia officinalis, and Satureja montana (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 Escherichia coli XL1-blue, and the antigenotoxicity on Salmonella typhimurium TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-O-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhib...ited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with O. basilicum ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.
Кључне речи:
phenolic compounds / Lamiaceae / genoprotective effect / antitumor activity / antioxidant activity / antigenotoxicityИзвор:
Plants-Basel, 2021, 10, 11Издавач:
- MDPI, Basel
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200178 (Универзитет у Београду, Биолошки факултет) (RS-MESTD-inst-2020-200178)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200003 (Институт за проучавање лековитог биља 'Др Јосиф Панчић', Београд) (RS-MESTD-inst-2020-200003)
DOI: 10.3390/plants10112306
ISSN: 2223-7747
PubMed: 34834669
WoS: 000725847300001
Scopus: 2-s2.0-85117933885
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Pavlović, Mariana Oalde AU - Kolarević, Stoimir AU - Đorđević Aleksić, Jelena AU - Jovanović-Marić, Jovana AU - Lunic, Tanja AU - Mandic, Marija AU - Kracun-Kolarević, Margareta AU - Živković, Jelena AU - Alimpic-Aradski, Ana AU - Marin, Petar D. AU - Savikin, Katarina AU - Vukovic-Gacic, Branka AU - Bozić-Nedeljković, Biljana AU - Duletic-Lausević, Sonja PY - 2021 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1502 AB - This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of Melissa officinalis, Mentha x piperita, Ocimum basilicum, Rosmarinus officinalis, Salvia officinalis, and Satureja montana (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 Escherichia coli XL1-blue, and the antigenotoxicity on Salmonella typhimurium TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-O-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with O. basilicum ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation. PB - MDPI, Basel T2 - Plants-Basel T1 - A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species IS - 11 VL - 10 DO - 10.3390/plants10112306 ER -
@article{ author = "Pavlović, Mariana Oalde and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Jovanović-Marić, Jovana and Lunic, Tanja and Mandic, Marija and Kracun-Kolarević, Margareta and Živković, Jelena and Alimpic-Aradski, Ana and Marin, Petar D. and Savikin, Katarina and Vukovic-Gacic, Branka and Bozić-Nedeljković, Biljana and Duletic-Lausević, Sonja", year = "2021", abstract = "This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of Melissa officinalis, Mentha x piperita, Ocimum basilicum, Rosmarinus officinalis, Salvia officinalis, and Satureja montana (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 Escherichia coli XL1-blue, and the antigenotoxicity on Salmonella typhimurium TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-O-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with O. basilicum ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.", publisher = "MDPI, Basel", journal = "Plants-Basel", title = "A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species", number = "11", volume = "10", doi = "10.3390/plants10112306" }
Pavlović, M. O., Kolarević, S., Đorđević Aleksić, J., Jovanović-Marić, J., Lunic, T., Mandic, M., Kracun-Kolarević, M., Živković, J., Alimpic-Aradski, A., Marin, P. D., Savikin, K., Vukovic-Gacic, B., Bozić-Nedeljković, B.,& Duletic-Lausević, S.. (2021). A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species. in Plants-Basel MDPI, Basel., 10(11). https://doi.org/10.3390/plants10112306
Pavlović MO, Kolarević S, Đorđević Aleksić J, Jovanović-Marić J, Lunic T, Mandic M, Kracun-Kolarević M, Živković J, Alimpic-Aradski A, Marin PD, Savikin K, Vukovic-Gacic B, Bozić-Nedeljković B, Duletic-Lausević S. A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species. in Plants-Basel. 2021;10(11). doi:10.3390/plants10112306 .
Pavlović, Mariana Oalde, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Jovanović-Marić, Jovana, Lunic, Tanja, Mandic, Marija, Kracun-Kolarević, Margareta, Živković, Jelena, Alimpic-Aradski, Ana, Marin, Petar D., Savikin, Katarina, Vukovic-Gacic, Branka, Bozić-Nedeljković, Biljana, Duletic-Lausević, Sonja, "A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species" in Plants-Basel, 10, no. 11 (2021), https://doi.org/10.3390/plants10112306 . .