Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin
2019
Преузимање 🢃
Аутори
Drvenica, Ivana T.Stancic, Ana Z.
Kalusević, Ana M.
Marković, Smilja
Dragišić Maksimović, Jelena
Nedović, Viktor A.
Bugarski, Branko M.
Ilic, Vesna Lj
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Slaughterhouse blood represents a valuable source of hemoglobin, which can be used in the production of heme-iron based supplements for the prevention/treatment of iron-deficiency anemia. In order to obtain a stable solid-state formulation, the effect of maltose addition (30 %) on the stability and storage of bovine and porcine hemoglobin in powders obtained by spray-and freeze-drying (without maltose: Hb; with maltose: HbM) were investigated. Differential scanning calorimetry of spray- and freeze-dried powders indicated satisfying quality of the formulation prepared with maltose on dissolving back into solution. After two-year storage at room temperature (20 +/- 5 degrees C) in solid forms, protected from moisture and light, rehydrated spray- and freeze- dried HbM were red, while Hb were brown. Dynamic light scattering showed the presence of native hemoglobin monomers in rehydrated spray- and freeze- dried HbM, but their agglomerates in Hb samples. UV-Vis spectrophotometry confirmed a...n absence of significant hemoglobin denaturation and methemoglobin formation in HbM freeze-dried powders. In spray-dried HbM, an increased level of methemoglobin was detected. The results confirmed the stabilizing effect of maltose, and suggested its use in the production of long-term stable solid-state formulations of hemoglobin, along with drying processes optimization.
Кључне речи:
UV-Vis spectroscopy / slaughterhouse blood / heme-iron protein / dynamic light scatteringИзвор:
Journal of the Serbian Chemical Society, 2019, 84, 10, 1105-1117Издавач:
- Srpsko hemijsko društvo, Beograd
Финансирање / пројекти:
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
DOI: 10.2298/JSC190513067D
ISSN: 0352-5139
WoS: 000496149600005
Scopus: 2-s2.0-85074904247
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Drvenica, Ivana T. AU - Stancic, Ana Z. AU - Kalusević, Ana M. AU - Marković, Smilja AU - Dragišić Maksimović, Jelena AU - Nedović, Viktor A. AU - Bugarski, Branko M. AU - Ilic, Vesna Lj PY - 2019 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1270 AB - Slaughterhouse blood represents a valuable source of hemoglobin, which can be used in the production of heme-iron based supplements for the prevention/treatment of iron-deficiency anemia. In order to obtain a stable solid-state formulation, the effect of maltose addition (30 %) on the stability and storage of bovine and porcine hemoglobin in powders obtained by spray-and freeze-drying (without maltose: Hb; with maltose: HbM) were investigated. Differential scanning calorimetry of spray- and freeze-dried powders indicated satisfying quality of the formulation prepared with maltose on dissolving back into solution. After two-year storage at room temperature (20 +/- 5 degrees C) in solid forms, protected from moisture and light, rehydrated spray- and freeze- dried HbM were red, while Hb were brown. Dynamic light scattering showed the presence of native hemoglobin monomers in rehydrated spray- and freeze- dried HbM, but their agglomerates in Hb samples. UV-Vis spectrophotometry confirmed an absence of significant hemoglobin denaturation and methemoglobin formation in HbM freeze-dried powders. In spray-dried HbM, an increased level of methemoglobin was detected. The results confirmed the stabilizing effect of maltose, and suggested its use in the production of long-term stable solid-state formulations of hemoglobin, along with drying processes optimization. PB - Srpsko hemijsko društvo, Beograd T2 - Journal of the Serbian Chemical Society T1 - Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin EP - 1117 IS - 10 SP - 1105 VL - 84 DO - 10.2298/JSC190513067D ER -
@article{ author = "Drvenica, Ivana T. and Stancic, Ana Z. and Kalusević, Ana M. and Marković, Smilja and Dragišić Maksimović, Jelena and Nedović, Viktor A. and Bugarski, Branko M. and Ilic, Vesna Lj", year = "2019", abstract = "Slaughterhouse blood represents a valuable source of hemoglobin, which can be used in the production of heme-iron based supplements for the prevention/treatment of iron-deficiency anemia. In order to obtain a stable solid-state formulation, the effect of maltose addition (30 %) on the stability and storage of bovine and porcine hemoglobin in powders obtained by spray-and freeze-drying (without maltose: Hb; with maltose: HbM) were investigated. Differential scanning calorimetry of spray- and freeze-dried powders indicated satisfying quality of the formulation prepared with maltose on dissolving back into solution. After two-year storage at room temperature (20 +/- 5 degrees C) in solid forms, protected from moisture and light, rehydrated spray- and freeze- dried HbM were red, while Hb were brown. Dynamic light scattering showed the presence of native hemoglobin monomers in rehydrated spray- and freeze- dried HbM, but their agglomerates in Hb samples. UV-Vis spectrophotometry confirmed an absence of significant hemoglobin denaturation and methemoglobin formation in HbM freeze-dried powders. In spray-dried HbM, an increased level of methemoglobin was detected. The results confirmed the stabilizing effect of maltose, and suggested its use in the production of long-term stable solid-state formulations of hemoglobin, along with drying processes optimization.", publisher = "Srpsko hemijsko društvo, Beograd", journal = "Journal of the Serbian Chemical Society", title = "Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin", pages = "1117-1105", number = "10", volume = "84", doi = "10.2298/JSC190513067D" }
Drvenica, I. T., Stancic, A. Z., Kalusević, A. M., Marković, S., Dragišić Maksimović, J., Nedović, V. A., Bugarski, B. M.,& Ilic, V. L.. (2019). Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin. in Journal of the Serbian Chemical Society Srpsko hemijsko društvo, Beograd., 84(10), 1105-1117. https://doi.org/10.2298/JSC190513067D
Drvenica IT, Stancic AZ, Kalusević AM, Marković S, Dragišić Maksimović J, Nedović VA, Bugarski BM, Ilic VL. Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin. in Journal of the Serbian Chemical Society. 2019;84(10):1105-1117. doi:10.2298/JSC190513067D .
Drvenica, Ivana T., Stancic, Ana Z., Kalusević, Ana M., Marković, Smilja, Dragišić Maksimović, Jelena, Nedović, Viktor A., Bugarski, Branko M., Ilic, Vesna Lj, "Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin" in Journal of the Serbian Chemical Society, 84, no. 10 (2019):1105-1117, https://doi.org/10.2298/JSC190513067D . .