Potential of Teucrium chamaedrys L. to modulate apoptosis and biotransformation in colorectal carcinoma cells
Само за регистроване кориснике
2019
Аутори
Milutinović, MilenaMaksimović, Vuk
Cvetković, Danijela M.
Nikodijević, Danijela D.
Stanković, Milan
Pesic, Milica
Marković, Snežana D.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Ethnopharmacological relevance: Teucrum chamaedrys L. is one of the known medicinal plants, useful for treatment of various health problems, especially digestive. In this study, we investigated methanol, ethyl-acetate and acetone extracts of T. chamaedrys in respect to their anticancer properties in SW480 colorectal cancer cells. Materials and methods: Cytotoxicity and proapoptotic potential were assessed by MTT cell viability assay and AO/EB double staining. Molecular mechanisms of induced apoptosis were determined by monitoring Fas receptor protein expression through immunofluorescence, Caspase 8 and 9 activity, as well as concentrations of O-2(center dot-) spectrophotometrically. Additionally, mRNA expression of biotransformation enzymes (CYP1A1, CYP1B1, GSTP1) and membrane transporters (MRP1 and MRP2) involved in drug resistance were investigated by qPCR method. Qualitative analysis of individual phenolic compounds was performed by reversed phase HPLC-MS analysis. Results: Methanol... extract shows the best cytotoxicity and selectivity compared to ethyl-acetate and acetone extracts, mainly causing apoptosis of SW480 cells, without affecting normal HaCaT keratinocytes. The increased expression of Fas receptor protein and caspase 8 activity indicate that the death receptor-mediated pathway plays a crucial role in the observed apoptosis. The increased caspase 9 activity and O-2(center dot-) concentration suggest that mitochondria are also involved in the apoptosis. T. chamaedrys methanol extract inhibits mRNA expression of CYP1A1, CYPIBI, GSTP1, MRP1 and MRP2 in SW480 cells. Conclusions: Induction of apoptosis and inhibition of CYP1A1, CYP1B1, GSTP1, MRP1 and MRP2 mRNA expression implies that T. chamaedrys can serve as a valuable source of bioactive compounds as dietary supplements or selective anticancer agents, with the ability to induce apoptosis and modulate drug resistance in colorectal cancer cells.
Кључне речи:
Phytochemicals / Multidrug resistance / Food supplement / Colorectal cancer / ApoptosisИзвор:
Journal of Ethnopharmacology, 2019, 240Издавач:
- Elsevier Ireland Ltd, Clare
Финансирање / пројекти:
- Преклиничка испитивања биоактивних супстанци (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41010)
DOI: 10.1016/j.jep.2019.111951
ISSN: 0378-8741
PubMed: 31085226
WoS: 000472984600008
Scopus: 2-s2.0-85065711907
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Milutinović, Milena AU - Maksimović, Vuk AU - Cvetković, Danijela M. AU - Nikodijević, Danijela D. AU - Stanković, Milan AU - Pesic, Milica AU - Marković, Snežana D. PY - 2019 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1213 AB - Ethnopharmacological relevance: Teucrum chamaedrys L. is one of the known medicinal plants, useful for treatment of various health problems, especially digestive. In this study, we investigated methanol, ethyl-acetate and acetone extracts of T. chamaedrys in respect to their anticancer properties in SW480 colorectal cancer cells. Materials and methods: Cytotoxicity and proapoptotic potential were assessed by MTT cell viability assay and AO/EB double staining. Molecular mechanisms of induced apoptosis were determined by monitoring Fas receptor protein expression through immunofluorescence, Caspase 8 and 9 activity, as well as concentrations of O-2(center dot-) spectrophotometrically. Additionally, mRNA expression of biotransformation enzymes (CYP1A1, CYP1B1, GSTP1) and membrane transporters (MRP1 and MRP2) involved in drug resistance were investigated by qPCR method. Qualitative analysis of individual phenolic compounds was performed by reversed phase HPLC-MS analysis. Results: Methanol extract shows the best cytotoxicity and selectivity compared to ethyl-acetate and acetone extracts, mainly causing apoptosis of SW480 cells, without affecting normal HaCaT keratinocytes. The increased expression of Fas receptor protein and caspase 8 activity indicate that the death receptor-mediated pathway plays a crucial role in the observed apoptosis. The increased caspase 9 activity and O-2(center dot-) concentration suggest that mitochondria are also involved in the apoptosis. T. chamaedrys methanol extract inhibits mRNA expression of CYP1A1, CYPIBI, GSTP1, MRP1 and MRP2 in SW480 cells. Conclusions: Induction of apoptosis and inhibition of CYP1A1, CYP1B1, GSTP1, MRP1 and MRP2 mRNA expression implies that T. chamaedrys can serve as a valuable source of bioactive compounds as dietary supplements or selective anticancer agents, with the ability to induce apoptosis and modulate drug resistance in colorectal cancer cells. PB - Elsevier Ireland Ltd, Clare T2 - Journal of Ethnopharmacology T1 - Potential of Teucrium chamaedrys L. to modulate apoptosis and biotransformation in colorectal carcinoma cells VL - 240 DO - 10.1016/j.jep.2019.111951 ER -
@article{ author = "Milutinović, Milena and Maksimović, Vuk and Cvetković, Danijela M. and Nikodijević, Danijela D. and Stanković, Milan and Pesic, Milica and Marković, Snežana D.", year = "2019", abstract = "Ethnopharmacological relevance: Teucrum chamaedrys L. is one of the known medicinal plants, useful for treatment of various health problems, especially digestive. In this study, we investigated methanol, ethyl-acetate and acetone extracts of T. chamaedrys in respect to their anticancer properties in SW480 colorectal cancer cells. Materials and methods: Cytotoxicity and proapoptotic potential were assessed by MTT cell viability assay and AO/EB double staining. Molecular mechanisms of induced apoptosis were determined by monitoring Fas receptor protein expression through immunofluorescence, Caspase 8 and 9 activity, as well as concentrations of O-2(center dot-) spectrophotometrically. Additionally, mRNA expression of biotransformation enzymes (CYP1A1, CYP1B1, GSTP1) and membrane transporters (MRP1 and MRP2) involved in drug resistance were investigated by qPCR method. Qualitative analysis of individual phenolic compounds was performed by reversed phase HPLC-MS analysis. Results: Methanol extract shows the best cytotoxicity and selectivity compared to ethyl-acetate and acetone extracts, mainly causing apoptosis of SW480 cells, without affecting normal HaCaT keratinocytes. The increased expression of Fas receptor protein and caspase 8 activity indicate that the death receptor-mediated pathway plays a crucial role in the observed apoptosis. The increased caspase 9 activity and O-2(center dot-) concentration suggest that mitochondria are also involved in the apoptosis. T. chamaedrys methanol extract inhibits mRNA expression of CYP1A1, CYPIBI, GSTP1, MRP1 and MRP2 in SW480 cells. Conclusions: Induction of apoptosis and inhibition of CYP1A1, CYP1B1, GSTP1, MRP1 and MRP2 mRNA expression implies that T. chamaedrys can serve as a valuable source of bioactive compounds as dietary supplements or selective anticancer agents, with the ability to induce apoptosis and modulate drug resistance in colorectal cancer cells.", publisher = "Elsevier Ireland Ltd, Clare", journal = "Journal of Ethnopharmacology", title = "Potential of Teucrium chamaedrys L. to modulate apoptosis and biotransformation in colorectal carcinoma cells", volume = "240", doi = "10.1016/j.jep.2019.111951" }
Milutinović, M., Maksimović, V., Cvetković, D. M., Nikodijević, D. D., Stanković, M., Pesic, M.,& Marković, S. D.. (2019). Potential of Teucrium chamaedrys L. to modulate apoptosis and biotransformation in colorectal carcinoma cells. in Journal of Ethnopharmacology Elsevier Ireland Ltd, Clare., 240. https://doi.org/10.1016/j.jep.2019.111951
Milutinović M, Maksimović V, Cvetković DM, Nikodijević DD, Stanković M, Pesic M, Marković SD. Potential of Teucrium chamaedrys L. to modulate apoptosis and biotransformation in colorectal carcinoma cells. in Journal of Ethnopharmacology. 2019;240. doi:10.1016/j.jep.2019.111951 .
Milutinović, Milena, Maksimović, Vuk, Cvetković, Danijela M., Nikodijević, Danijela D., Stanković, Milan, Pesic, Milica, Marković, Snežana D., "Potential of Teucrium chamaedrys L. to modulate apoptosis and biotransformation in colorectal carcinoma cells" in Journal of Ethnopharmacology, 240 (2019), https://doi.org/10.1016/j.jep.2019.111951 . .