A one-step staining protocol for in-gel fluorescent visualization of proteins
Апстракт
Native polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate (SDS)–PAGE are among the most frequently applied techniques in protein analysis. Here we describe a fast one-step method for fluorescent visualization of proteins. Following PAGE, gels are soaked in solution of potassium ferricyanide (100 mM) in 1 M NaOH, and are kept in the dark for 30 min. Gels are then transferred to water and scanned. The sensitivity of the method is comparable with standard Coomassie Brilliant Blue staining.
Кључне речи:
Tryptophan / Staining protocol / PAGE / Fluorescence / Ferricyanide / ElectrophoresisИзвор:
Methods in Molecular Biology, 2018, 1853, 149-158Издавач:
- Humana Press Inc.
DOI: 10.1007/978-1-4939-8745-0_18
ISBN: 978-1-4939-8744-3
ISSN: 1064-3745
PubMed: 30097940
Scopus: 2-s2.0-85051741191
Институција/група
Institut za multidisciplinarna istraživanjaTY - CHAP AU - Bogdanović Pristov, Jelena AU - Spasojević, Ivan PY - 2018 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1176 AB - Native polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate (SDS)–PAGE are among the most frequently applied techniques in protein analysis. Here we describe a fast one-step method for fluorescent visualization of proteins. Following PAGE, gels are soaked in solution of potassium ferricyanide (100 mM) in 1 M NaOH, and are kept in the dark for 30 min. Gels are then transferred to water and scanned. The sensitivity of the method is comparable with standard Coomassie Brilliant Blue staining. PB - Humana Press Inc. T2 - Methods in Molecular Biology T1 - A one-step staining protocol for in-gel fluorescent visualization of proteins EP - 158 SP - 149 VL - 1853 DO - 10.1007/978-1-4939-8745-0_18 ER -
@inbook{ author = "Bogdanović Pristov, Jelena and Spasojević, Ivan", year = "2018", abstract = "Native polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate (SDS)–PAGE are among the most frequently applied techniques in protein analysis. Here we describe a fast one-step method for fluorescent visualization of proteins. Following PAGE, gels are soaked in solution of potassium ferricyanide (100 mM) in 1 M NaOH, and are kept in the dark for 30 min. Gels are then transferred to water and scanned. The sensitivity of the method is comparable with standard Coomassie Brilliant Blue staining.", publisher = "Humana Press Inc.", journal = "Methods in Molecular Biology", booktitle = "A one-step staining protocol for in-gel fluorescent visualization of proteins", pages = "158-149", volume = "1853", doi = "10.1007/978-1-4939-8745-0_18" }
Bogdanović Pristov, J.,& Spasojević, I.. (2018). A one-step staining protocol for in-gel fluorescent visualization of proteins. in Methods in Molecular Biology Humana Press Inc.., 1853, 149-158. https://doi.org/10.1007/978-1-4939-8745-0_18
Bogdanović Pristov J, Spasojević I. A one-step staining protocol for in-gel fluorescent visualization of proteins. in Methods in Molecular Biology. 2018;1853:149-158. doi:10.1007/978-1-4939-8745-0_18 .
Bogdanović Pristov, Jelena, Spasojević, Ivan, "A one-step staining protocol for in-gel fluorescent visualization of proteins" in Methods in Molecular Biology, 1853 (2018):149-158, https://doi.org/10.1007/978-1-4939-8745-0_18 . .