TY  - JOUR
AU  - Mattoo, Autar
AU  - Cavigelli, Michel
AU  - Mišić, Danijela
AU  - Gašić, Uroš
AU  - Maksimović, Vuk
AU  - Kramer, Matthew
AU  - Kaur, Bhavneet
AU  - Matekalo, Dragana
AU  - Nestorović Živković, Jasmina
AU  - Roberts, Daniel
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2198
AB  - Maize is important to global food security, being one of the predominant cereals in human and domesticated livestock diets worldwide. Due to the increasing human population, it will be important to not only design cropping systems to increase maize yield and sustainability but also to improve the nutritional quality of maize edible tissues. To determine cropping system impacts on maize grain nutritional content, we sampled grain from conventional and organic maize varieties grown for three growing seasons using five cropping systems. We analyzed the grain using metabolic fingerprinting of methanol extracts with ultra-high performance liquid chromatography (UHPLC) coupled with mass spectrometry (MS), adopting both non-targeted and targeted approaches. The cropping systems are part of a long-term study at the Beltsville Agricultural Research Center in Beltsville, Maryland, and were a three-year conventional no-till rotation (NT), a three-year conventional chisel-till rotation (CT), a two-year organic rotation (Org2), a three-year organic rotation (Org3), and a six-year organic rotation (Org6). Each cropping system had been in place for at least 10 years, allowing specific cropping-system-induced alterations of soil edaphic and microbial properties. Non-targeted metabolic fingerprinting detected a total of 90 compounds, the majority of which were phenolics. Metabolic profiling was further targeted toward 15 phenolics, 1 phytohormone, 7 carbohydrates and 7 organic acids, which were quantified in the maize grain originating from the five cropping systems. Statistical analysis of this subset of quantitative data determined that cropping system can significantly influence levels of certain maize grain metabolites. However, natural impacts (growing year) were substantially greater than cropping system impacts, likely masking or over-riding some cropping system impacts. Additionally, maize cultivar genetics had greater impact than cropping system on the maize grain metabolome and was the greatest “managed” impact on the metabolite profiles. Results indicate that until natural environmental impacts on maize grain metabolite levels are understood and managed, the best approach to reliably increase maize grain nutritional quality is through development of maize cultivars with enhanced nutritional content that are robust to natural environmental influence.
PB  - Frontiers Media SA
T2  - Frontiers in Sustainable Food Systems
T1  - Maize metabolomics in relation to cropping system and growing year
EP  - 13
SP  - 1
VL  - 7
DO  - 10.3389/fsufs.2023.1130089
ER  - 

@article{
author = "Mattoo, Autar and Cavigelli, Michel and Mišić, Danijela and Gašić, Uroš and Maksimović, Vuk and Kramer, Matthew and Kaur, Bhavneet and Matekalo, Dragana and Nestorović Živković, Jasmina and Roberts, Daniel",
year = "2023",
abstract = "Maize is important to global food security, being one of the predominant cereals in human and domesticated livestock diets worldwide. Due to the increasing human population, it will be important to not only design cropping systems to increase maize yield and sustainability but also to improve the nutritional quality of maize edible tissues. To determine cropping system impacts on maize grain nutritional content, we sampled grain from conventional and organic maize varieties grown for three growing seasons using five cropping systems. We analyzed the grain using metabolic fingerprinting of methanol extracts with ultra-high performance liquid chromatography (UHPLC) coupled with mass spectrometry (MS), adopting both non-targeted and targeted approaches. The cropping systems are part of a long-term study at the Beltsville Agricultural Research Center in Beltsville, Maryland, and were a three-year conventional no-till rotation (NT), a three-year conventional chisel-till rotation (CT), a two-year organic rotation (Org2), a three-year organic rotation (Org3), and a six-year organic rotation (Org6). Each cropping system had been in place for at least 10 years, allowing specific cropping-system-induced alterations of soil edaphic and microbial properties. Non-targeted metabolic fingerprinting detected a total of 90 compounds, the majority of which were phenolics. Metabolic profiling was further targeted toward 15 phenolics, 1 phytohormone, 7 carbohydrates and 7 organic acids, which were quantified in the maize grain originating from the five cropping systems. Statistical analysis of this subset of quantitative data determined that cropping system can significantly influence levels of certain maize grain metabolites. However, natural impacts (growing year) were substantially greater than cropping system impacts, likely masking or over-riding some cropping system impacts. Additionally, maize cultivar genetics had greater impact than cropping system on the maize grain metabolome and was the greatest “managed” impact on the metabolite profiles. Results indicate that until natural environmental impacts on maize grain metabolite levels are understood and managed, the best approach to reliably increase maize grain nutritional quality is through development of maize cultivars with enhanced nutritional content that are robust to natural environmental influence.",
publisher = "Frontiers Media SA",
journal = "Frontiers in Sustainable Food Systems",
title = "Maize metabolomics in relation to cropping system and growing year",
pages = "13-1",
volume = "7",
doi = "10.3389/fsufs.2023.1130089"
}

Mattoo, A., Cavigelli, M., Mišić, D., Gašić, U., Maksimović, V., Kramer, M., Kaur, B., Matekalo, D., Nestorović Živković, J.,& Roberts, D.. (2023). Maize metabolomics in relation to cropping system and growing year. in Frontiers in Sustainable Food Systems
Frontiers Media SA., 7, 1-13.
https://doi.org/10.3389/fsufs.2023.1130089

Mattoo A, Cavigelli M, Mišić D, Gašić U, Maksimović V, Kramer M, Kaur B, Matekalo D, Nestorović Živković J, Roberts D. Maize metabolomics in relation to cropping system and growing year. in Frontiers in Sustainable Food Systems. 2023;7:1-13.
doi:10.3389/fsufs.2023.1130089 .

Mattoo, Autar, Cavigelli, Michel, Mišić, Danijela, Gašić, Uroš, Maksimović, Vuk, Kramer, Matthew, Kaur, Bhavneet, Matekalo, Dragana, Nestorović Živković, Jasmina, Roberts, Daniel, "Maize metabolomics in relation to cropping system and growing year" in Frontiers in Sustainable Food Systems, 7 (2023):1-13,
https://doi.org/10.3389/fsufs.2023.1130089 . .

TY  - JOUR
AU  - Vuković-Gačić, Branka
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Joksimović, Danijela
AU  - Martinović, Rajko
AU  - Ramšak, Andreja
AU  - Bajt, Oliver
AU  - Đorđević Aleksić, Jelena
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2160
AB  - In this study, the possible ‘vector effect’ within the exposure of Mediterranean mussels (Mytilus galloprovincialis) to polystyrene microplastics with adsorbed fluoranthene was investigated by applying the multibiomarker approach. The major focus was placed on genotoxicological endpoints as to our knowledge there are no literature data on the genotoxicity of polystyrene microparticles alone or with adsorbed fluoranthene in the selected experimental organisms. DNA damage was assessed in haemocytes by comet assay and micronucleus test. For the assess- ment of neurotoxicity, acetylcholinesterase activity was measured in gills. Glutathione S-transferase was assessed in gills and hepatopancreas since these enzymes are induced for biotransformation and excretion of lipophilic compounds such as hydrocarbons. Finally, differences in physiological response within the exposure to polystyrene particles, fluoranthene, or particles with adsorbed fluoranthene were assessed by the variation of heart rate patterns studied by the noninvasive laser fibre-optic method. The uniform response of individual biomarkers within the exposure groups was not recorded. There was no clear pattern in variation of acetylcholinesterase or glutathione S-transferase activity which could be attributed to the treatment. Exposure to polystyrene increased DNA damage which was detected by the comet assay but was not confirmed by micronucleus formation. Data of genotoxicity assays indicated differential responses among the groups exposed to fluoranthene alone and fluoranthene adsorbed to polystyrene. Change in the heart rate patterns within the studied groups supports the concept of the Trojan horse effect within the exposure to polystyrene particles with adsorbed fluoranthene.
PB  - Oxford University Press
T2  - Mutagenesis
T1  - Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis)
EP  - 12
IS  - 38
SP  - 3
VL  - 1
DO  - 10.1093/mutage/geac017
ER  - 

@article{
author = "Vuković-Gačić, Branka and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Joksimović, Danijela and Martinović, Rajko and Ramšak, Andreja and Bajt, Oliver and Đorđević Aleksić, Jelena",
year = "2023",
abstract = "In this study, the possible ‘vector effect’ within the exposure of Mediterranean mussels (Mytilus galloprovincialis) to polystyrene microplastics with adsorbed fluoranthene was investigated by applying the multibiomarker approach. The major focus was placed on genotoxicological endpoints as to our knowledge there are no literature data on the genotoxicity of polystyrene microparticles alone or with adsorbed fluoranthene in the selected experimental organisms. DNA damage was assessed in haemocytes by comet assay and micronucleus test. For the assess- ment of neurotoxicity, acetylcholinesterase activity was measured in gills. Glutathione S-transferase was assessed in gills and hepatopancreas since these enzymes are induced for biotransformation and excretion of lipophilic compounds such as hydrocarbons. Finally, differences in physiological response within the exposure to polystyrene particles, fluoranthene, or particles with adsorbed fluoranthene were assessed by the variation of heart rate patterns studied by the noninvasive laser fibre-optic method. The uniform response of individual biomarkers within the exposure groups was not recorded. There was no clear pattern in variation of acetylcholinesterase or glutathione S-transferase activity which could be attributed to the treatment. Exposure to polystyrene increased DNA damage which was detected by the comet assay but was not confirmed by micronucleus formation. Data of genotoxicity assays indicated differential responses among the groups exposed to fluoranthene alone and fluoranthene adsorbed to polystyrene. Change in the heart rate patterns within the studied groups supports the concept of the Trojan horse effect within the exposure to polystyrene particles with adsorbed fluoranthene.",
publisher = "Oxford University Press",
journal = "Mutagenesis",
title = "Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis)",
pages = "12-3",
number = "38",
volume = "1",
doi = "10.1093/mutage/geac017"
}

Vuković-Gačić, B., Kolarević, S., Kračun-Kolarević, M., Jovanović Marić, J., Joksimović, D., Martinović, R., Ramšak, A., Bajt, O.,& Đorđević Aleksić, J.. (2023). Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis). in Mutagenesis
Oxford University Press., 1(38), 3-12.
https://doi.org/10.1093/mutage/geac017

Vuković-Gačić B, Kolarević S, Kračun-Kolarević M, Jovanović Marić J, Joksimović D, Martinović R, Ramšak A, Bajt O, Đorđević Aleksić J. Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis). in Mutagenesis. 2023;1(38):3-12.
doi:10.1093/mutage/geac017 .

Vuković-Gačić, Branka, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Joksimović, Danijela, Martinović, Rajko, Ramšak, Andreja, Bajt, Oliver, Đorđević Aleksić, Jelena, "Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis)" in Mutagenesis, 1, no. 38 (2023):3-12,
https://doi.org/10.1093/mutage/geac017 . .

TY  - JOUR
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Sunjog, Karolina
AU  - Nikolić, Ivan
AU  - Marić, Ana
AU  - Ilić, Marija
AU  - Simonović, Predrag
AU  - Alygizakis, Nikiforos
AU  - Ng, Kilsey
AU  - Oswald, Petar
AU  - Slobodnik, Jaroslav
AU  - Žegura, Bojana
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
AU  - Kračun-Kolarević, Margareta
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2044
AB  - Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.
PB  - Oxford University Press
T2  - Mutagenesis
T1  - In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study
EP  - 32
IS  - 1
SP  - 21
VL  - 38
DO  - 10.1093/mutage/geac024
ER  - 

@article{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Sunjog, Karolina and Nikolić, Ivan and Marić, Ana and Ilić, Marija and Simonović, Predrag and Alygizakis, Nikiforos and Ng, Kilsey and Oswald, Petar and Slobodnik, Jaroslav and Žegura, Bojana and Vuković-Gačić, Branka and Paunović, Momir and Kračun-Kolarević, Margareta",
year = "2023",
abstract = "Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.",
publisher = "Oxford University Press",
journal = "Mutagenesis",
title = "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study",
pages = "32-21",
number = "1",
volume = "38",
doi = "10.1093/mutage/geac024"
}

Jovanović Marić, J., Kolarević, S., Đorđević Aleksić, J., Sunjog, K., Nikolić, I., Marić, A., Ilić, M., Simonović, P., Alygizakis, N., Ng, K., Oswald, P., Slobodnik, J., Žegura, B., Vuković-Gačić, B., Paunović, M.,& Kračun-Kolarević, M.. (2023). In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis
Oxford University Press., 38(1), 21-32.
https://doi.org/10.1093/mutage/geac024

Jovanović Marić J, Kolarević S, Đorđević Aleksić J, Sunjog K, Nikolić I, Marić A, Ilić M, Simonović P, Alygizakis N, Ng K, Oswald P, Slobodnik J, Žegura B, Vuković-Gačić B, Paunović M, Kračun-Kolarević M. In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis. 2023;38(1):21-32.
doi:10.1093/mutage/geac024 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Sunjog, Karolina, Nikolić, Ivan, Marić, Ana, Ilić, Marija, Simonović, Predrag, Alygizakis, Nikiforos, Ng, Kilsey, Oswald, Petar, Slobodnik, Jaroslav, Žegura, Bojana, Vuković-Gačić, Branka, Paunović, Momir, Kračun-Kolarević, Margareta, "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study" in Mutagenesis, 38, no. 1 (2023):21-32,
https://doi.org/10.1093/mutage/geac024 . .