TY  - JOUR
AU  - Simonović, Mladen
AU  - Mix, Thorsten
AU  - Glumac, Miodrag
AU  - Pejin, Boris
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1547
AB  - A new protein detector of 2,4,6-trinitrophenol (TNP, picric acid) is concisely reported herein for the first time. In brief, the gene of a specific scFv fragment, namely 3.5, was fused separately with the gene of beta-lactamase and, subsequently, expressed in the periplasmic space of Escherichia coli, affording in such a way the recombinant fusion protein 3.5-scFv-beta-lactamase. This bifunctional immunoreagent tool was further convincingly demonstrated for being capable to directly detect trinitrophenol-tris(hydroxymethyl)aminomethane (TNP-Tris), a less toxic TNP derivative of choice compared to TNP itself, with competitive sensitivity (50 +/- 2 fmol or 175 +/- 6 pg/mL).
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - 3.5-scFv-beta-lactamase, a new protein detector of picric acid, the highly nitrated organic explosive
EP  - 1320
IS  - 5
SP  - 1317
VL  - 36
DO  - 10.1080/14786419.2020.1860977
ER  - 

@article{
author = "Simonović, Mladen and Mix, Thorsten and Glumac, Miodrag and Pejin, Boris",
year = "2022",
abstract = "A new protein detector of 2,4,6-trinitrophenol (TNP, picric acid) is concisely reported herein for the first time. In brief, the gene of a specific scFv fragment, namely 3.5, was fused separately with the gene of beta-lactamase and, subsequently, expressed in the periplasmic space of Escherichia coli, affording in such a way the recombinant fusion protein 3.5-scFv-beta-lactamase. This bifunctional immunoreagent tool was further convincingly demonstrated for being capable to directly detect trinitrophenol-tris(hydroxymethyl)aminomethane (TNP-Tris), a less toxic TNP derivative of choice compared to TNP itself, with competitive sensitivity (50 +/- 2 fmol or 175 +/- 6 pg/mL).",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "3.5-scFv-beta-lactamase, a new protein detector of picric acid, the highly nitrated organic explosive",
pages = "1320-1317",
number = "5",
volume = "36",
doi = "10.1080/14786419.2020.1860977"
}

Simonović, M., Mix, T., Glumac, M.,& Pejin, B.. (2022). 3.5-scFv-beta-lactamase, a new protein detector of picric acid, the highly nitrated organic explosive. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 36(5), 1317-1320.
https://doi.org/10.1080/14786419.2020.1860977

Simonović M, Mix T, Glumac M, Pejin B. 3.5-scFv-beta-lactamase, a new protein detector of picric acid, the highly nitrated organic explosive. in Natural Product Research. 2022;36(5):1317-1320.
doi:10.1080/14786419.2020.1860977 .

Simonović, Mladen, Mix, Thorsten, Glumac, Miodrag, Pejin, Boris, "3.5-scFv-beta-lactamase, a new protein detector of picric acid, the highly nitrated organic explosive" in Natural Product Research, 36, no. 5 (2022):1317-1320,
https://doi.org/10.1080/14786419.2020.1860977 . .

TY  - JOUR
AU  - Simonović, Mladen
AU  - Ostojic, Sanja
AU  - Micic, Darko
AU  - Bisercic-Savić, Marjetka
AU  - Mix, Thorsten
AU  - Glumac, Miodrag
AU  - Pejin, Boris
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1537
AB  - This study aimed to develop a fast and reliable protocol for Trinitrophenol-Tris(hydroxymethyl)aminomethane (TNP-Tris) detection applying a beta-lactamase-fusion protein of choice, the natural product-based immunoreagent tool of competitive sensitivity developed herein for the first time. Since the fusion protein 11B3-scFv-beta-lactamase is constructed from a scFv-antibody (11B3) linked to an enzyme (beta-lactamase), the step related to the use of secondary antibody in the enzyme-linked immunosorbent assay (ELISA) is completely omitted. Indeed, this fusion protein itself serves both as binding mean of the antigen model and detecting agent, due to the presence of the naturally occurring enzyme. In such a way, it actually affords the one-step TNP-Tris detection reaching a promising LOD value of 45 +/- 2 fmol or 157 +/- 6 pg/mL. Taken all together, the current protocol does represent much cheaper and significantly less-time consuming alternative compared both to the recombinant antibodies and recombinant phages, previously designed means in our labs for the same purpose. [GRAPHICS] .
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - A novel and effective natural product-based immunodetection tool for TNT-like compounds
EP  - 861
IS  - 3
SP  - 857
VL  - 36
DO  - 10.1080/14786419.2020.1806269
ER  - 

@article{
author = "Simonović, Mladen and Ostojic, Sanja and Micic, Darko and Bisercic-Savić, Marjetka and Mix, Thorsten and Glumac, Miodrag and Pejin, Boris",
year = "2022",
abstract = "This study aimed to develop a fast and reliable protocol for Trinitrophenol-Tris(hydroxymethyl)aminomethane (TNP-Tris) detection applying a beta-lactamase-fusion protein of choice, the natural product-based immunoreagent tool of competitive sensitivity developed herein for the first time. Since the fusion protein 11B3-scFv-beta-lactamase is constructed from a scFv-antibody (11B3) linked to an enzyme (beta-lactamase), the step related to the use of secondary antibody in the enzyme-linked immunosorbent assay (ELISA) is completely omitted. Indeed, this fusion protein itself serves both as binding mean of the antigen model and detecting agent, due to the presence of the naturally occurring enzyme. In such a way, it actually affords the one-step TNP-Tris detection reaching a promising LOD value of 45 +/- 2 fmol or 157 +/- 6 pg/mL. Taken all together, the current protocol does represent much cheaper and significantly less-time consuming alternative compared both to the recombinant antibodies and recombinant phages, previously designed means in our labs for the same purpose. [GRAPHICS] .",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "A novel and effective natural product-based immunodetection tool for TNT-like compounds",
pages = "861-857",
number = "3",
volume = "36",
doi = "10.1080/14786419.2020.1806269"
}

Simonović, M., Ostojic, S., Micic, D., Bisercic-Savić, M., Mix, T., Glumac, M.,& Pejin, B.. (2022). A novel and effective natural product-based immunodetection tool for TNT-like compounds. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 36(3), 857-861.
https://doi.org/10.1080/14786419.2020.1806269

Simonović M, Ostojic S, Micic D, Bisercic-Savić M, Mix T, Glumac M, Pejin B. A novel and effective natural product-based immunodetection tool for TNT-like compounds. in Natural Product Research. 2022;36(3):857-861.
doi:10.1080/14786419.2020.1806269 .

Simonović, Mladen, Ostojic, Sanja, Micic, Darko, Bisercic-Savić, Marjetka, Mix, Thorsten, Glumac, Miodrag, Pejin, Boris, "A novel and effective natural product-based immunodetection tool for TNT-like compounds" in Natural Product Research, 36, no. 3 (2022):857-861,
https://doi.org/10.1080/14786419.2020.1806269 . .

TY  - JOUR
AU  - Draca, Dijana
AU  - Mijatović, Sanja
AU  - Krajnović, Tamara
AU  - Bogdanović Pristov, Jelena
AU  - Dukic, Tatjana
AU  - Kaluderović, Goran N.
AU  - Wessjohann, Ludger A.
AU  - Maksimović-Ivanić, Danijela
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1228
AB  - Synthetic tubugis are equally potent but more stable than their natural forms. Their anticancer potential was estimated on a solid melanoma in vitro and in vivo. Tubugi-1 induced the apoptosis in B16 cells accompanied with strong intracellular production of reactive species, subsequently imposing glutathione and thiol group depletion. Paradoxically, membrane lipids were excluded from the cascade of intracellular oxidation, according to malondialdehyde decrease. Although morphologically apoptosis was typical, externalization of phosphatidylserine (PS) as an early apoptotic event was not detected. Even their exposition is pivotal for apoptotic cell eradication, primary macrophages successfully eliminated PS-deficient tubugi-1 induced apoptotic cells. The tumor volume in animals exposed to the drug in therapeutic mode was reduced in comparison to control as well as to paclitaxeltreated animals Importantly, macrophages isolated from tubugi-1 treated animals possessed conserved phagocytic activity and were functionally and phenotypically recognized as Ml. The cytotoxic effect of tubugi-1 is accomplished through its ability to polarize the macrophages toward Ml, probably by PS independent apoptotic cell engulfment. The unique potential of tubugi-1 to prime the innate immune response through the induction of a specific pattern of tumor cell apoptosis can be of extraordinary importance from fundamental and applicable aspects.
PB  - Elsevier Inc, San Diego
T2  - Experimental Cell Research
T1  - The synthetic tubulysin derivative, tubugi-1, improves the innate immune response by macrophage polarization in addition to its direct cytotoxic effects in a murine melanoma model
EP  - 170
IS  - 2
SP  - 159
VL  - 380
DO  - 10.1016/j.yexcr.2019.04.028
ER  - 

@article{
author = "Draca, Dijana and Mijatović, Sanja and Krajnović, Tamara and Bogdanović Pristov, Jelena and Dukic, Tatjana and Kaluderović, Goran N. and Wessjohann, Ludger A. and Maksimović-Ivanić, Danijela",
year = "2019",
abstract = "Synthetic tubugis are equally potent but more stable than their natural forms. Their anticancer potential was estimated on a solid melanoma in vitro and in vivo. Tubugi-1 induced the apoptosis in B16 cells accompanied with strong intracellular production of reactive species, subsequently imposing glutathione and thiol group depletion. Paradoxically, membrane lipids were excluded from the cascade of intracellular oxidation, according to malondialdehyde decrease. Although morphologically apoptosis was typical, externalization of phosphatidylserine (PS) as an early apoptotic event was not detected. Even their exposition is pivotal for apoptotic cell eradication, primary macrophages successfully eliminated PS-deficient tubugi-1 induced apoptotic cells. The tumor volume in animals exposed to the drug in therapeutic mode was reduced in comparison to control as well as to paclitaxeltreated animals Importantly, macrophages isolated from tubugi-1 treated animals possessed conserved phagocytic activity and were functionally and phenotypically recognized as Ml. The cytotoxic effect of tubugi-1 is accomplished through its ability to polarize the macrophages toward Ml, probably by PS independent apoptotic cell engulfment. The unique potential of tubugi-1 to prime the innate immune response through the induction of a specific pattern of tumor cell apoptosis can be of extraordinary importance from fundamental and applicable aspects.",
publisher = "Elsevier Inc, San Diego",
journal = "Experimental Cell Research",
title = "The synthetic tubulysin derivative, tubugi-1, improves the innate immune response by macrophage polarization in addition to its direct cytotoxic effects in a murine melanoma model",
pages = "170-159",
number = "2",
volume = "380",
doi = "10.1016/j.yexcr.2019.04.028"
}

Draca, D., Mijatović, S., Krajnović, T., Bogdanović Pristov, J., Dukic, T., Kaluderović, G. N., Wessjohann, L. A.,& Maksimović-Ivanić, D.. (2019). The synthetic tubulysin derivative, tubugi-1, improves the innate immune response by macrophage polarization in addition to its direct cytotoxic effects in a murine melanoma model. in Experimental Cell Research
Elsevier Inc, San Diego., 380(2), 159-170.
https://doi.org/10.1016/j.yexcr.2019.04.028

Draca D, Mijatović S, Krajnović T, Bogdanović Pristov J, Dukic T, Kaluderović GN, Wessjohann LA, Maksimović-Ivanić D. The synthetic tubulysin derivative, tubugi-1, improves the innate immune response by macrophage polarization in addition to its direct cytotoxic effects in a murine melanoma model. in Experimental Cell Research. 2019;380(2):159-170.
doi:10.1016/j.yexcr.2019.04.028 .

Draca, Dijana, Mijatović, Sanja, Krajnović, Tamara, Bogdanović Pristov, Jelena, Dukic, Tatjana, Kaluderović, Goran N., Wessjohann, Ludger A., Maksimović-Ivanić, Danijela, "The synthetic tubulysin derivative, tubugi-1, improves the innate immune response by macrophage polarization in addition to its direct cytotoxic effects in a murine melanoma model" in Experimental Cell Research, 380, no. 2 (2019):159-170,
https://doi.org/10.1016/j.yexcr.2019.04.028 . .