TY  - CONF
AU  - Knezevic, Magdalena
AU  - Jelušić, Aleksandra
AU  - Buntić, Aneta
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2119
AB  - The use of microbial inoculants as an alternative to chemical pesticides holds significant value in modern agriculture, addressing the growing demand for sustainable and environmentally friendly pest management strategies. Fusarium oxysporum is a plant pathogenic fungus that poses substantial challenges to global agriculture, mostly due to its broad host range and persistence in soil. Therefore, the aim of this research was to find potent bacterial isolate(s) for managing
infection caused by this plant pathogen. Twelve bacterial isolates (coded as PAM1.1 – PAM1.12), obtained from saline soil were used in this research. Their ability to produce protease and cellulase, as well as their antifungal potential against F. oxysporum were tested in vitro. DNA of the most effective isolate was extracted using the CTAB extraction protocol and amplified with primer pair P0/P6 targeting bacterial 16S rRNA gene. For identification, the obtained sequences
were compared to those available in the National Center for Biotechnology Information (NCBI) database. Out of twelve tested isolates, PAM1.3 showed the highest proteolytic activity, while the production of cellulase was not detected. This isolate was concurrently the most efficient in suppressing F. oxysporum, with an inhibition percentage of 51.7%. Based on the NCBI BLASTn analysis of the 16S rRNA gene sequence, isolate PAM1.3 was identified as Stenotrophomonas
lactitubi, showing 99.29% identity with S. lactitubi strain NR_179509.1. The results of this research indicate that saline soils could be a valuable reservoir of beneficial bacteria with biocontrol properties.
PB  - University of Belgrade, Faculty of Biology
C3  - ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture
T1  - Bacteria from saline soil as promising biocontrol agents against Fusarium oxysporum
SP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2119
ER  - 

@conference{
author = "Knezevic, Magdalena and Jelušić, Aleksandra and Buntić, Aneta",
year = "2023",
abstract = "The use of microbial inoculants as an alternative to chemical pesticides holds significant value in modern agriculture, addressing the growing demand for sustainable and environmentally friendly pest management strategies. Fusarium oxysporum is a plant pathogenic fungus that poses substantial challenges to global agriculture, mostly due to its broad host range and persistence in soil. Therefore, the aim of this research was to find potent bacterial isolate(s) for managing
infection caused by this plant pathogen. Twelve bacterial isolates (coded as PAM1.1 – PAM1.12), obtained from saline soil were used in this research. Their ability to produce protease and cellulase, as well as their antifungal potential against F. oxysporum were tested in vitro. DNA of the most effective isolate was extracted using the CTAB extraction protocol and amplified with primer pair P0/P6 targeting bacterial 16S rRNA gene. For identification, the obtained sequences
were compared to those available in the National Center for Biotechnology Information (NCBI) database. Out of twelve tested isolates, PAM1.3 showed the highest proteolytic activity, while the production of cellulase was not detected. This isolate was concurrently the most efficient in suppressing F. oxysporum, with an inhibition percentage of 51.7%. Based on the NCBI BLASTn analysis of the 16S rRNA gene sequence, isolate PAM1.3 was identified as Stenotrophomonas
lactitubi, showing 99.29% identity with S. lactitubi strain NR_179509.1. The results of this research indicate that saline soils could be a valuable reservoir of beneficial bacteria with biocontrol properties.",
publisher = "University of Belgrade, Faculty of Biology",
journal = "ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture",
title = "Bacteria from saline soil as promising biocontrol agents against Fusarium oxysporum",
pages = "80",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2119"
}

Knezevic, M., Jelušić, A.,& Buntić, A.. (2023). Bacteria from saline soil as promising biocontrol agents against Fusarium oxysporum. in ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture
University of Belgrade, Faculty of Biology., 80.
https://hdl.handle.net/21.15107/rcub_rimsi_2119

Knezevic M, Jelušić A, Buntić A. Bacteria from saline soil as promising biocontrol agents against Fusarium oxysporum. in ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture. 2023;:80.
https://hdl.handle.net/21.15107/rcub_rimsi_2119 .

Knezevic, Magdalena, Jelušić, Aleksandra, Buntić, Aneta, "Bacteria from saline soil as promising biocontrol agents against Fusarium oxysporum" in ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture (2023):80,
https://hdl.handle.net/21.15107/rcub_rimsi_2119 .