TY  - CONF
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Marković, Sanja
AU  - Živković, Natalija
AU  - Dimkić, Ivica
AU  - Nikolić, Ivan
AU  - Stanković, Slaviša
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1929
AB  - Recent studies have been made to investigate some molecular features of plant pathogenic 
bacteria Pseudomonas syringae pv. coriandricola. This pathogen causes bacterial leaf spot 
disease on some members of family Apiaceae, such as coriander, carrot, parsnip and parsley. 
Initial symptoms are small water-soaked lesions on foliage, which then develop into spots 
varying in shades, tan, brown or black. The spots are usually limited by leaf veins having an 
angular appearance, and visible from both sides of leaves. Under favorable conditions of high 
humidity, spots may spread and cause foliage blighting. P. s. pv. coriandricola strains used in this 
study originate from carrot, parsley and parsnip. Repetitive element PCR fingerprinting (rep PCR), using REP, BOX, ERIC, (GTG)5 and SERE primers, and randomly amplified polymorphic 
DNA-polymerase chain reaction (RAPD-PCR) using M13 primer were used to determinate 
genetic polymorphism between tested strains. Further molecular features were observed on the 
basis of a multilocus sequence analysis (MLSA) by sequencing key genes for Pseudomonas 
syringae (gapA, gltA, gyrB, rpoD). DNA profiling revealed identical banding patterns for all 
tested strains. The sequence and phylogenetic analysis of three MLSA genes (gapA, gyrB, rpoD) 
showed homology with pathotype strains P. s. pv. coriandricola deposited in PAMDB 
(http://genome.ppws.vt.edu/cgi-bin/MLST/home.pl). Sequencing of gltA gene determined the 
isolates above the species level (P. syringae). Our results indicate that gapA, gyrB and rpoD 
genes are more discriminatory then gltA and their usage is therefore suggested for further 
genotypic studies of this pathogen.
PB  - INTERNATIONAL Scientific Agricultural Symposium "Agrosym 2017"
C3  - VIII International Scientific Agriculture Symposium “AGROSYM 2017”
T1  - MOLECULAR CHARACTERIZATION OF PSEUDOMONAS SYRINGAE PV.  CORIANDRICOLA ORIGINATED FROM CARROT, PARSLEY AND PARSNIP
SP  - 549
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1929
ER  - 

@conference{
author = "Popović, Tatjana and Jelušić, Aleksandra and Marković, Sanja and Živković, Natalija and Dimkić, Ivica and Nikolić, Ivan and Stanković, Slaviša",
year = "2017",
abstract = "Recent studies have been made to investigate some molecular features of plant pathogenic 
bacteria Pseudomonas syringae pv. coriandricola. This pathogen causes bacterial leaf spot 
disease on some members of family Apiaceae, such as coriander, carrot, parsnip and parsley. 
Initial symptoms are small water-soaked lesions on foliage, which then develop into spots 
varying in shades, tan, brown or black. The spots are usually limited by leaf veins having an 
angular appearance, and visible from both sides of leaves. Under favorable conditions of high 
humidity, spots may spread and cause foliage blighting. P. s. pv. coriandricola strains used in this 
study originate from carrot, parsley and parsnip. Repetitive element PCR fingerprinting (rep PCR), using REP, BOX, ERIC, (GTG)5 and SERE primers, and randomly amplified polymorphic 
DNA-polymerase chain reaction (RAPD-PCR) using M13 primer were used to determinate 
genetic polymorphism between tested strains. Further molecular features were observed on the 
basis of a multilocus sequence analysis (MLSA) by sequencing key genes for Pseudomonas 
syringae (gapA, gltA, gyrB, rpoD). DNA profiling revealed identical banding patterns for all 
tested strains. The sequence and phylogenetic analysis of three MLSA genes (gapA, gyrB, rpoD) 
showed homology with pathotype strains P. s. pv. coriandricola deposited in PAMDB 
(http://genome.ppws.vt.edu/cgi-bin/MLST/home.pl). Sequencing of gltA gene determined the 
isolates above the species level (P. syringae). Our results indicate that gapA, gyrB and rpoD 
genes are more discriminatory then gltA and their usage is therefore suggested for further 
genotypic studies of this pathogen.",
publisher = "INTERNATIONAL Scientific Agricultural Symposium "Agrosym 2017"",
journal = "VIII International Scientific Agriculture Symposium “AGROSYM 2017”",
title = "MOLECULAR CHARACTERIZATION OF PSEUDOMONAS SYRINGAE PV.  CORIANDRICOLA ORIGINATED FROM CARROT, PARSLEY AND PARSNIP",
pages = "549",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1929"
}

Popović, T., Jelušić, A., Marković, S., Živković, N., Dimkić, I., Nikolić, I.,& Stanković, S.. (2017). MOLECULAR CHARACTERIZATION OF PSEUDOMONAS SYRINGAE PV.  CORIANDRICOLA ORIGINATED FROM CARROT, PARSLEY AND PARSNIP. in VIII International Scientific Agriculture Symposium “AGROSYM 2017”
INTERNATIONAL Scientific Agricultural Symposium "Agrosym 2017"., 549.
https://hdl.handle.net/21.15107/rcub_rimsi_1929

Popović T, Jelušić A, Marković S, Živković N, Dimkić I, Nikolić I, Stanković S. MOLECULAR CHARACTERIZATION OF PSEUDOMONAS SYRINGAE PV.  CORIANDRICOLA ORIGINATED FROM CARROT, PARSLEY AND PARSNIP. in VIII International Scientific Agriculture Symposium “AGROSYM 2017”. 2017;:549.
https://hdl.handle.net/21.15107/rcub_rimsi_1929 .

Popović, Tatjana, Jelušić, Aleksandra, Marković, Sanja, Živković, Natalija, Dimkić, Ivica, Nikolić, Ivan, Stanković, Slaviša, "MOLECULAR CHARACTERIZATION OF PSEUDOMONAS SYRINGAE PV.  CORIANDRICOLA ORIGINATED FROM CARROT, PARSLEY AND PARSNIP" in VIII International Scientific Agriculture Symposium “AGROSYM 2017” (2017):549,
https://hdl.handle.net/21.15107/rcub_rimsi_1929 .