TY  - CONF
AU  - Marković, Sanja
AU  - Popović, Tatjana
AU  - Stanković, Slaviša
AU  - Jelušić, Aleksandra
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1928
AB  - BACKGROUNDS: Ralstonia solanacearum race 3 biovar 2 is one of the most important plant 
pathogenic bacteria because of its persistence, wide host range and widespread. It´s causing 
bacterial wilt of over 250 plant species, including many cultivated crops (potato, tomato, eggplant, 
geranium, ginger, banana, etc.) and brown rot of potato.
OBJECTIVES: The aim of this study was to determine presence of R. solanacearum based on 
several years monitoring of potato tubers.
METHODS: During four-year period (2013-2016), 98 samples of potato tubers (11 cultivars), 
from 13 localities in Bačka and 1 in Podunavlje region were examined. Isolation from potato 
tubers with brown rot symptom was performed on semi-selective SMSA medium. PCR method 
using Ps-1/Ps-2 and OLY1/Y2 primers and immunofluorescence were used to detect presence of 
R. solanacearum. Pathogenicity was tested on young tomato plants.
RESULTS: In 2013, R. solanacearum was detected in 1 sample of potato tuber (Lady Claire) from 
Srpski Miletić; in 2015 in 2 (Crisps4all, VR 808) from Srpski Miletić and 1 (VR 808) from Stapar; 
during 2016 in 2 (Crisps4all, Brooke) from Srpski Miletić, 2 (Lady Claire, VR 808) from Stapar, 2 
(Lady Claire, Pirol) from Sombor and 1 (Panda) from Boleč. Milky-white, with pink to red centre, 
flat, irregular, fluidal colonies formed after 3-4 days of incubation on SMSA medium. A 553 bp and 
288 bp fragments of 65 representative isolates were amplified using Ps-1/Ps-2 and OLY1/Y2 
primer pairs, respectively. IF test proved presence of fluorescent bacterial cells. All strains caused 
wilting on tomato seedlings four days after inoculation.
CONCLUSIONS: The results of this study detected presence of R. solanacearum on 11 potato 
samples isolated from four locations during period from 2013-2016.
PB  - Udruženje mikrobiologa Srbije
C3  - 12TH CONGRESS OF SERBIAN MICROBIOLOGISTS  with international participation MICROMED 2018 REGIO
T1  - MONITORING OF POTATO FIELDS TO PRESENCE OF RALSTONIA SOLANACEARUM
EP  - 210
SP  - 209
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1928
ER  - 

@conference{
author = "Marković, Sanja and Popović, Tatjana and Stanković, Slaviša and Jelušić, Aleksandra",
year = "2018",
abstract = "BACKGROUNDS: Ralstonia solanacearum race 3 biovar 2 is one of the most important plant 
pathogenic bacteria because of its persistence, wide host range and widespread. It´s causing 
bacterial wilt of over 250 plant species, including many cultivated crops (potato, tomato, eggplant, 
geranium, ginger, banana, etc.) and brown rot of potato.
OBJECTIVES: The aim of this study was to determine presence of R. solanacearum based on 
several years monitoring of potato tubers.
METHODS: During four-year period (2013-2016), 98 samples of potato tubers (11 cultivars), 
from 13 localities in Bačka and 1 in Podunavlje region were examined. Isolation from potato 
tubers with brown rot symptom was performed on semi-selective SMSA medium. PCR method 
using Ps-1/Ps-2 and OLY1/Y2 primers and immunofluorescence were used to detect presence of 
R. solanacearum. Pathogenicity was tested on young tomato plants.
RESULTS: In 2013, R. solanacearum was detected in 1 sample of potato tuber (Lady Claire) from 
Srpski Miletić; in 2015 in 2 (Crisps4all, VR 808) from Srpski Miletić and 1 (VR 808) from Stapar; 
during 2016 in 2 (Crisps4all, Brooke) from Srpski Miletić, 2 (Lady Claire, VR 808) from Stapar, 2 
(Lady Claire, Pirol) from Sombor and 1 (Panda) from Boleč. Milky-white, with pink to red centre, 
flat, irregular, fluidal colonies formed after 3-4 days of incubation on SMSA medium. A 553 bp and 
288 bp fragments of 65 representative isolates were amplified using Ps-1/Ps-2 and OLY1/Y2 
primer pairs, respectively. IF test proved presence of fluorescent bacterial cells. All strains caused 
wilting on tomato seedlings four days after inoculation.
CONCLUSIONS: The results of this study detected presence of R. solanacearum on 11 potato 
samples isolated from four locations during period from 2013-2016.",
publisher = "Udruženje mikrobiologa Srbije",
journal = "12TH CONGRESS OF SERBIAN MICROBIOLOGISTS  with international participation MICROMED 2018 REGIO",
title = "MONITORING OF POTATO FIELDS TO PRESENCE OF RALSTONIA SOLANACEARUM",
pages = "210-209",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1928"
}

Marković, S., Popović, T., Stanković, S.,& Jelušić, A.. (2018). MONITORING OF POTATO FIELDS TO PRESENCE OF RALSTONIA SOLANACEARUM. in 12TH CONGRESS OF SERBIAN MICROBIOLOGISTS  with international participation MICROMED 2018 REGIO
Udruženje mikrobiologa Srbije., 209-210.
https://hdl.handle.net/21.15107/rcub_rimsi_1928

Marković S, Popović T, Stanković S, Jelušić A. MONITORING OF POTATO FIELDS TO PRESENCE OF RALSTONIA SOLANACEARUM. in 12TH CONGRESS OF SERBIAN MICROBIOLOGISTS  with international participation MICROMED 2018 REGIO. 2018;:209-210.
https://hdl.handle.net/21.15107/rcub_rimsi_1928 .

Marković, Sanja, Popović, Tatjana, Stanković, Slaviša, Jelušić, Aleksandra, "MONITORING OF POTATO FIELDS TO PRESENCE OF RALSTONIA SOLANACEARUM" in 12TH CONGRESS OF SERBIAN MICROBIOLOGISTS  with international participation MICROMED 2018 REGIO (2018):209-210,
https://hdl.handle.net/21.15107/rcub_rimsi_1928 .