TY  - JOUR
AU  - Radotić, Ksenija
AU  - Stanković, Mira
AU  - Bartolić, Dragana
AU  - Natić, Maja
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2477
AB  - Food is a complex matrix of proteins, fats, minerals, vitamins, and other components. Various analytical methods are currently used for food testing. However, most of the used methods require sample preprocessing and expensive chemicals. New analytical methods are needed for quick and economic measurement of food quality and safety. Fluorescence spectroscopy is a simple and quick method to measure food quality, without sample preprocessing. This technique has been developed for food samples due to the application of a front-face measuring setup. Fluorescent compounds–fluorophores in the food samples are highly sensitive to their environment. Information about molecular structure and changes in food samples is obtained by the measurement of excitation–emission matrices of the endogenous fluorophores and by applying multivariate chemometric tools. Synchronous fluorescence spectroscopy is an advantageous screening mode used in food analysis. The fluorescent markers in food are amino acids tryptophan and tyrosine; the structural proteins collagen and elastin; the enzymes and co-enzymes NADH and FAD; vitamins; lipids; porphyrins; and mycotoxins in certain food types. The review provides information on the principles of the fluorescence measurements of food samples and the advantages of this method over the others. An analysis of the fluorescence spectroscopy applications in screening the various food types is provided.
PB  - MDPI
T2  - Foods
T1  - Intrinsic Fluorescence Markers for Food Characteristics, Shelf Life, and Safety Estimation: Advanced Analytical Approach
EP  - 17
IS  - 16
SP  - 1
VL  - 12
DO  - 10.3390/foods12163023
ER  - 

@article{
author = "Radotić, Ksenija and Stanković, Mira and Bartolić, Dragana and Natić, Maja",
year = "2023",
abstract = "Food is a complex matrix of proteins, fats, minerals, vitamins, and other components. Various analytical methods are currently used for food testing. However, most of the used methods require sample preprocessing and expensive chemicals. New analytical methods are needed for quick and economic measurement of food quality and safety. Fluorescence spectroscopy is a simple and quick method to measure food quality, without sample preprocessing. This technique has been developed for food samples due to the application of a front-face measuring setup. Fluorescent compounds–fluorophores in the food samples are highly sensitive to their environment. Information about molecular structure and changes in food samples is obtained by the measurement of excitation–emission matrices of the endogenous fluorophores and by applying multivariate chemometric tools. Synchronous fluorescence spectroscopy is an advantageous screening mode used in food analysis. The fluorescent markers in food are amino acids tryptophan and tyrosine; the structural proteins collagen and elastin; the enzymes and co-enzymes NADH and FAD; vitamins; lipids; porphyrins; and mycotoxins in certain food types. The review provides information on the principles of the fluorescence measurements of food samples and the advantages of this method over the others. An analysis of the fluorescence spectroscopy applications in screening the various food types is provided.",
publisher = "MDPI",
journal = "Foods",
title = "Intrinsic Fluorescence Markers for Food Characteristics, Shelf Life, and Safety Estimation: Advanced Analytical Approach",
pages = "17-1",
number = "16",
volume = "12",
doi = "10.3390/foods12163023"
}

Radotić, K., Stanković, M., Bartolić, D.,& Natić, M.. (2023). Intrinsic Fluorescence Markers for Food Characteristics, Shelf Life, and Safety Estimation: Advanced Analytical Approach. in Foods
MDPI., 12(16), 1-17.
https://doi.org/10.3390/foods12163023

Radotić K, Stanković M, Bartolić D, Natić M. Intrinsic Fluorescence Markers for Food Characteristics, Shelf Life, and Safety Estimation: Advanced Analytical Approach. in Foods. 2023;12(16):1-17.
doi:10.3390/foods12163023 .

Radotić, Ksenija, Stanković, Mira, Bartolić, Dragana, Natić, Maja, "Intrinsic Fluorescence Markers for Food Characteristics, Shelf Life, and Safety Estimation: Advanced Analytical Approach" in Foods, 12, no. 16 (2023):1-17,
https://doi.org/10.3390/foods12163023 . .

TY  - JOUR
AU  - Pastor, Ferenc
AU  - Dojčinović, Biljana P.
AU  - Kodranov, Igor D.
AU  - Gorjanović, Stanislava Ž.
AU  - Ivanović, Tijana
AU  - Popović, Daniela Ž.
AU  - Miladinović, Jelena
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3095
AB  - Due to the similar chemical properties of Ni2+ and Co2+, several dozens of adsorptive stripping voltammetric (AdSV)
methods have been developed for their simultaneous determination. The question was would there be some benefits
if a stripping method made for simultaneous determination of Ni2+ and Co2+ will be optimized for the determination
of only one of them (Ni2+). It was found that the optimized method has for an order of magnitude lower LOQ (1.89 ×
10-10 M), an order of magnitude lower influence of Co2+, and the applicability of one calibration line for four orders
of magnitude of Ni2+ concentration. The influence of some common anions and cations has been examined. The
developed method was successfully applied for nickel content determination in real samples. The suitability of the
developed method for the determination of Co2+ from the same solution in the second run, upon optimizations of
deposition potential and time, was also explored. The LOQ obtained for Co2+ (3.61 × 10-11 M) is almost two orders
of magnitude lower than LOQ of the method for simultaneous determination of both cations and, its LOD and LOQ
are among few lowest obtained by AdSV methods for Co2+ determination. The developed method for Ni2+ and Co2+
determination from the same solution in two successive runs has significantly better analytical performances than
the starting method for their simultaneous determination in one run.
PB  - John Wiley and Sons Inc.
T2  - Electroanalysis
T1  - 2 in 1versus 1plus 1 – Outcomes of the transformation of adsorptive stripping method for the Ni2+ and Co2+ determination
DO  - 10.1002/elan.202300259
ER  - 

@article{
author = "Pastor, Ferenc and Dojčinović, Biljana P. and Kodranov, Igor D. and Gorjanović, Stanislava Ž. and Ivanović, Tijana and Popović, Daniela Ž. and Miladinović, Jelena",
year = "2023",
abstract = "Due to the similar chemical properties of Ni2+ and Co2+, several dozens of adsorptive stripping voltammetric (AdSV)
methods have been developed for their simultaneous determination. The question was would there be some benefits
if a stripping method made for simultaneous determination of Ni2+ and Co2+ will be optimized for the determination
of only one of them (Ni2+). It was found that the optimized method has for an order of magnitude lower LOQ (1.89 ×
10-10 M), an order of magnitude lower influence of Co2+, and the applicability of one calibration line for four orders
of magnitude of Ni2+ concentration. The influence of some common anions and cations has been examined. The
developed method was successfully applied for nickel content determination in real samples. The suitability of the
developed method for the determination of Co2+ from the same solution in the second run, upon optimizations of
deposition potential and time, was also explored. The LOQ obtained for Co2+ (3.61 × 10-11 M) is almost two orders
of magnitude lower than LOQ of the method for simultaneous determination of both cations and, its LOD and LOQ
are among few lowest obtained by AdSV methods for Co2+ determination. The developed method for Ni2+ and Co2+
determination from the same solution in two successive runs has significantly better analytical performances than
the starting method for their simultaneous determination in one run.",
publisher = "John Wiley and Sons Inc.",
journal = "Electroanalysis",
title = "2 in 1versus 1plus 1 – Outcomes of the transformation of adsorptive stripping method for the Ni2+ and Co2+ determination",
doi = "10.1002/elan.202300259"
}

Pastor, F., Dojčinović, B. P., Kodranov, I. D., Gorjanović, S. Ž., Ivanović, T., Popović, D. Ž.,& Miladinović, J.. (2023). 2 in 1versus 1plus 1 – Outcomes of the transformation of adsorptive stripping method for the Ni2+ and Co2+ determination. in Electroanalysis
John Wiley and Sons Inc...
https://doi.org/10.1002/elan.202300259

Pastor F, Dojčinović BP, Kodranov ID, Gorjanović SŽ, Ivanović T, Popović DŽ, Miladinović J. 2 in 1versus 1plus 1 – Outcomes of the transformation of adsorptive stripping method for the Ni2+ and Co2+ determination. in Electroanalysis. 2023;.
doi:10.1002/elan.202300259 .

Pastor, Ferenc, Dojčinović, Biljana P., Kodranov, Igor D., Gorjanović, Stanislava Ž., Ivanović, Tijana, Popović, Daniela Ž., Miladinović, Jelena, "2 in 1versus 1plus 1 – Outcomes of the transformation of adsorptive stripping method for the Ni2+ and Co2+ determination" in Electroanalysis (2023),
https://doi.org/10.1002/elan.202300259 . .

TY  - JOUR
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Sikoparija, Branko
AU  - Nedic, Nebojsa
AU  - Andric, Filip
AU  - Natalija, Polović
AU  - Natić, Maja
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1781
AB  - Varroa destructor is a parasitic mite responsible for the loss of honey bee (Apis mellifera)
colonies. This study aimed to find a promising marker in honey for the bee colony infestation level
using fluorescence spectroscopy and biochemical analyses. We examined whether the parameters of
the honey samples’ fluorescence spectra and biochemical parameters, both related to proteins and
phenolics, may be connected with the level of honey bee colonies’ infestation. The infestation level
was highly positively correlated with the catalase activity in honey (r = 0.936). Additionally, the
infestation level was positively correlated with the phenolic spectral component (r = 0.656), which
was tentatively related to the phenolics in honey. No correlation was found between the diastase
activity in honey and the colonies’ infestation level. The results indicate that the catalase activity in
honey and the PFC1 spectral component may be reliable markers for the V. destructor infestation level
of the colonies. The obtained data may be related to the honey yield obtained from the apiaries.
PB  - MDPI
T2  - Foods
T1  - Using Front-Face Fluorescence Spectroscopy and Biochemical Analysis of Honey to Assess a Marker for the Level of Varroa destructor Infestation of Honey Bee (Apis mellifera) Colonies
EP  - 13
SP  - 629
SP  - 1
VL  - 12
DO  - 10.3390/foods12030629
ER  - 

@article{
author = "Stanković, Mira and Prokopijević, Miloš and Sikoparija, Branko and Nedic, Nebojsa and Andric, Filip and Natalija, Polović and Natić, Maja and Radotić, Ksenija",
year = "2023",
abstract = "Varroa destructor is a parasitic mite responsible for the loss of honey bee (Apis mellifera)
colonies. This study aimed to find a promising marker in honey for the bee colony infestation level
using fluorescence spectroscopy and biochemical analyses. We examined whether the parameters of
the honey samples’ fluorescence spectra and biochemical parameters, both related to proteins and
phenolics, may be connected with the level of honey bee colonies’ infestation. The infestation level
was highly positively correlated with the catalase activity in honey (r = 0.936). Additionally, the
infestation level was positively correlated with the phenolic spectral component (r = 0.656), which
was tentatively related to the phenolics in honey. No correlation was found between the diastase
activity in honey and the colonies’ infestation level. The results indicate that the catalase activity in
honey and the PFC1 spectral component may be reliable markers for the V. destructor infestation level
of the colonies. The obtained data may be related to the honey yield obtained from the apiaries.",
publisher = "MDPI",
journal = "Foods",
title = "Using Front-Face Fluorescence Spectroscopy and Biochemical Analysis of Honey to Assess a Marker for the Level of Varroa destructor Infestation of Honey Bee (Apis mellifera) Colonies",
pages = "13-629-1",
volume = "12",
doi = "10.3390/foods12030629"
}

Stanković, M., Prokopijević, M., Sikoparija, B., Nedic, N., Andric, F., Natalija, P., Natić, M.,& Radotić, K.. (2023). Using Front-Face Fluorescence Spectroscopy and Biochemical Analysis of Honey to Assess a Marker for the Level of Varroa destructor Infestation of Honey Bee (Apis mellifera) Colonies. in Foods
MDPI., 12, 629-13.
https://doi.org/10.3390/foods12030629

Stanković M, Prokopijević M, Sikoparija B, Nedic N, Andric F, Natalija P, Natić M, Radotić K. Using Front-Face Fluorescence Spectroscopy and Biochemical Analysis of Honey to Assess a Marker for the Level of Varroa destructor Infestation of Honey Bee (Apis mellifera) Colonies. in Foods. 2023;12:629-13.
doi:10.3390/foods12030629 .

Stanković, Mira, Prokopijević, Miloš, Sikoparija, Branko, Nedic, Nebojsa, Andric, Filip, Natalija, Polović, Natić, Maja, Radotić, Ksenija, "Using Front-Face Fluorescence Spectroscopy and Biochemical Analysis of Honey to Assess a Marker for the Level of Varroa destructor Infestation of Honey Bee (Apis mellifera) Colonies" in Foods, 12 (2023):629-13,
https://doi.org/10.3390/foods12030629 . .

TY  - JOUR
AU  - Kalicanin, Nevena
AU  - Balaz, Ana Marija
AU  - Prodanović, Olivera
AU  - Prodanović, Radivoje
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2612
AB  - The aim of this research was to prove the function of the putative opine dehydrogenase from Desulfohalobium retbaense and to characterize the enzyme in terms of functional and kinetic parameters. A putative opine dehydrogenase was identified from a metagenomic library by a sequence-based technique search of the metagenomic library, and afterward was successfully heterologously produced in Escherichia coli. In order to examine its potential for applications in the synthesis of secondary amines, first the substrate specificity of the enzyme towards different amino donors and amino acceptors was determined. The highest affinity was observed towards small amino acids, preferentially L-alanine, and when it comes to α-keto acids, pyruvate proved to be a preferential amino acceptor. The highest activity was observed at pH 6.5 in the absence of salts. The enzyme showed remarkable stability in a wide range of experimental conditions, such as broad pH stability (from 6.0–11.0 after 30 min incubation in buffers at a certain pH), stability in the presence of NaCl up to 3.0 M for 24 h, it retained 80% of the initial activity after 1 h incubation at 45°C, and 65% of the initial activity after 24 h incubation in 30% dimethyl sulfoxide.
PB  - Wiley-VCH GmbH
T2  - ChemBioChem
T1  - Heterologous Expression and Partial Characterization of a Putative Opine Dehydrogenase from a Metagenomic Sequence of Desulfohalobium retbaense
IS  - 20
IS  - e202300414
VL  - 24
DO  - 10.1002/cbic.202300414
ER  - 

@article{
author = "Kalicanin, Nevena and Balaz, Ana Marija and Prodanović, Olivera and Prodanović, Radivoje",
year = "2023",
abstract = "The aim of this research was to prove the function of the putative opine dehydrogenase from Desulfohalobium retbaense and to characterize the enzyme in terms of functional and kinetic parameters. A putative opine dehydrogenase was identified from a metagenomic library by a sequence-based technique search of the metagenomic library, and afterward was successfully heterologously produced in Escherichia coli. In order to examine its potential for applications in the synthesis of secondary amines, first the substrate specificity of the enzyme towards different amino donors and amino acceptors was determined. The highest affinity was observed towards small amino acids, preferentially L-alanine, and when it comes to α-keto acids, pyruvate proved to be a preferential amino acceptor. The highest activity was observed at pH 6.5 in the absence of salts. The enzyme showed remarkable stability in a wide range of experimental conditions, such as broad pH stability (from 6.0–11.0 after 30 min incubation in buffers at a certain pH), stability in the presence of NaCl up to 3.0 M for 24 h, it retained 80% of the initial activity after 1 h incubation at 45°C, and 65% of the initial activity after 24 h incubation in 30% dimethyl sulfoxide.",
publisher = "Wiley-VCH GmbH",
journal = "ChemBioChem",
title = "Heterologous Expression and Partial Characterization of a Putative Opine Dehydrogenase from a Metagenomic Sequence of Desulfohalobium retbaense",
number = "20, e202300414",
volume = "24",
doi = "10.1002/cbic.202300414"
}

Kalicanin, N., Balaz, A. M., Prodanović, O.,& Prodanović, R.. (2023). Heterologous Expression and Partial Characterization of a Putative Opine Dehydrogenase from a Metagenomic Sequence of Desulfohalobium retbaense. in ChemBioChem
Wiley-VCH GmbH., 24(20).
https://doi.org/10.1002/cbic.202300414

Kalicanin N, Balaz AM, Prodanović O, Prodanović R. Heterologous Expression and Partial Characterization of a Putative Opine Dehydrogenase from a Metagenomic Sequence of Desulfohalobium retbaense. in ChemBioChem. 2023;24(20).
doi:10.1002/cbic.202300414 .

Kalicanin, Nevena, Balaz, Ana Marija, Prodanović, Olivera, Prodanović, Radivoje, "Heterologous Expression and Partial Characterization of a Putative Opine Dehydrogenase from a Metagenomic Sequence of Desulfohalobium retbaense" in ChemBioChem, 24, no. 20 (2023),
https://doi.org/10.1002/cbic.202300414 . .

TY  - JOUR
AU  - Krstajić Pajić, Mila N.
AU  - Dobrota, Ana S.
AU  - Mazare, Anca
AU  - Đurđić, Slađana
AU  - Hwang, Imgon
AU  - Skorodumova, Natalia V.
AU  - Manojlović, Dragan
AU  - Vasilic, Rastko
AU  - Pašti, Igor A.
AU  - Schmuki, Patrik
AU  - Lačnjevac, Uroš
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2706
AB  - Efficient cathodes for the hydrogen evolution reaction (HER) in acidic
water electrolysis rely on the use of expensive platinum group metals (PGMs). However, to
achieve economically viable operation, both the content of PGMs must be reduced and
their intrinsically strong H adsorption mitigated. Herein, we show that the surface effects of
hydrogenated TiO2 nanotube (TNT) arrays can make osmium, a so far less-explored PGM,
a highly active HER electrocatalyst. These defect-rich TiO2 nanostructures provide an
interactive scaffold for the galvanic deposition of Os particles with modulated adsorption
properties. Through systematic investigations, we identify the synthesis conditions (OsCl3
concentration/temperature/reaction time) that yield a progressive improvement in Os
deposition rate and mass loading, thereby decreasing the HER overpotential. At the same
time, the Os particles deposited by this procedure remain mainly sub-nanometric and
entirely cover the inner tube walls. An optimally balanced Os@TNT composite prepared at
3 mM/55 °C/30 min exhibits a record low overpotential (η) of 61 mV at a current density
of 100 mA cm−2, a high mass activity of 20.8 A mgOs
−1 at 80 mV, and a stable performance in an acidic medium. Density functional
theory calculations indicate the existence of strong interactions between the hydrogenated TiO2 surface and small Os clusters, which
may weaken the Os−H* binding strength and thus boost the intrinsic HER activity of Os centers. The results presented in this study
offer new directions for the fabrication of cost-effective PGM-based catalysts and a better understanding of the synergistic electronic
interactions at the PGM|TiO2 interface.
PB  - American Chemical Society
T2  - ACS Applied Materials and Interfaces
T1  - Activation of Osmium by the Surface Effects of Hydrogenated TiO2 Nanotube Arrays for Enhanced Hydrogen Evolution Reaction Performance
EP  - 31469
IS  - 26
SP  - 31459
VL  - 15
DO  - 10.1021/acsami.3c04498
ER  - 

@article{
author = "Krstajić Pajić, Mila N. and Dobrota, Ana S. and Mazare, Anca and Đurđić, Slađana and Hwang, Imgon and Skorodumova, Natalia V. and Manojlović, Dragan and Vasilic, Rastko and Pašti, Igor A. and Schmuki, Patrik and Lačnjevac, Uroš",
year = "2023",
abstract = "Efficient cathodes for the hydrogen evolution reaction (HER) in acidic
water electrolysis rely on the use of expensive platinum group metals (PGMs). However, to
achieve economically viable operation, both the content of PGMs must be reduced and
their intrinsically strong H adsorption mitigated. Herein, we show that the surface effects of
hydrogenated TiO2 nanotube (TNT) arrays can make osmium, a so far less-explored PGM,
a highly active HER electrocatalyst. These defect-rich TiO2 nanostructures provide an
interactive scaffold for the galvanic deposition of Os particles with modulated adsorption
properties. Through systematic investigations, we identify the synthesis conditions (OsCl3
concentration/temperature/reaction time) that yield a progressive improvement in Os
deposition rate and mass loading, thereby decreasing the HER overpotential. At the same
time, the Os particles deposited by this procedure remain mainly sub-nanometric and
entirely cover the inner tube walls. An optimally balanced Os@TNT composite prepared at
3 mM/55 °C/30 min exhibits a record low overpotential (η) of 61 mV at a current density
of 100 mA cm−2, a high mass activity of 20.8 A mgOs
−1 at 80 mV, and a stable performance in an acidic medium. Density functional
theory calculations indicate the existence of strong interactions between the hydrogenated TiO2 surface and small Os clusters, which
may weaken the Os−H* binding strength and thus boost the intrinsic HER activity of Os centers. The results presented in this study
offer new directions for the fabrication of cost-effective PGM-based catalysts and a better understanding of the synergistic electronic
interactions at the PGM|TiO2 interface.",
publisher = "American Chemical Society",
journal = "ACS Applied Materials and Interfaces",
title = "Activation of Osmium by the Surface Effects of Hydrogenated TiO2 Nanotube Arrays for Enhanced Hydrogen Evolution Reaction Performance",
pages = "31469-31459",
number = "26",
volume = "15",
doi = "10.1021/acsami.3c04498"
}

Krstajić Pajić, M. N., Dobrota, A. S., Mazare, A., Đurđić, S., Hwang, I., Skorodumova, N. V., Manojlović, D., Vasilic, R., Pašti, I. A., Schmuki, P.,& Lačnjevac, U.. (2023). Activation of Osmium by the Surface Effects of Hydrogenated TiO2 Nanotube Arrays for Enhanced Hydrogen Evolution Reaction Performance. in ACS Applied Materials and Interfaces
American Chemical Society., 15(26), 31459-31469.
https://doi.org/10.1021/acsami.3c04498

Krstajić Pajić MN, Dobrota AS, Mazare A, Đurđić S, Hwang I, Skorodumova NV, Manojlović D, Vasilic R, Pašti IA, Schmuki P, Lačnjevac U. Activation of Osmium by the Surface Effects of Hydrogenated TiO2 Nanotube Arrays for Enhanced Hydrogen Evolution Reaction Performance. in ACS Applied Materials and Interfaces. 2023;15(26):31459-31469.
doi:10.1021/acsami.3c04498 .

Krstajić Pajić, Mila N., Dobrota, Ana S., Mazare, Anca, Đurđić, Slađana, Hwang, Imgon, Skorodumova, Natalia V., Manojlović, Dragan, Vasilic, Rastko, Pašti, Igor A., Schmuki, Patrik, Lačnjevac, Uroš, "Activation of Osmium by the Surface Effects of Hydrogenated TiO2 Nanotube Arrays for Enhanced Hydrogen Evolution Reaction Performance" in ACS Applied Materials and Interfaces, 15, no. 26 (2023):31459-31469,
https://doi.org/10.1021/acsami.3c04498 . .

TY  - CONF
AU  - Banđur, Dunja
AU  - Milenković, Milica
AU  - Bartolić, Dragana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2310
AB  - Interactions of antibiotics with biometals can affect their antimicrobial activity by changing their bioavailability, redox properties, and stability toward hydrolysis. [1,2] The pH of biological fluids can significantly affect interactions between biometals and ionizable drugs. In this study, we investigated the interactions of Fe3+ with levofloxacin in water at different pH using fluorescence spectroscopy. The coordination of Fe3+ with levofloxacin is pH dependent due to the ionization of levofloxacin and solubility of Fe3+(aq) ions. The formation of levofloxacin-Fe3+ complex was detected in the pH range 4–5 as a decrease in fluorescence intensity of levofloxacin. The Stern-Volmer diagrams of levofloxacin in the presence of different concentrations of Fe3+ ion at pH 4 and pH 5 correspond to the static fluorescence quenching confirming the coordination of levofloxacin with high-spin Fe3+. At pH < 4 and pH > 5 the addition of FeCl3 as a source of Fe3+ ions results in an increase in fluorescence intensity which can be attributed to the change of the ionic strength of solution and electrostatic interactions between different ionic species instead of coordination of Fe3+ ions with levofloxacin. The coordination of Fe3+ with levofloxacin at pH < 4 is prevented by protonation of its carboxyl and piperazinyl groups (pKa1 = 6.02, pKa2 = 8.15). At pH > 5, despite the presence of zwitterionic and anionic forms of levofloxacin available for coordination, there is no complex formation due to the precipitation of insoluble ferric hydroxide.
References
1. Božić B, Korać J, Stanković DM, Stanić M, Romanović M, Pristov JB, Spasić S, Popović-Bijelić A, Spasojević I, Bajčetić M. Free Radical Biol. Med. 2018, 129, 279.
2. Chen J, Wang Y, Qian Y, Huang T. J. Hazard. Mater. 2017, 335, 117.
PB  - Serbian Chemical Society and Serbian Young Chemists’ Club,Karnegijeva 4/Ⅲ, 11000 Belgrade, Serbia
C3  - 9th Conference of Young Chemists of Serbia, Book of Abstracts, 2023,45, CB PP 14
T1  - Investigation of pH dependent Fe3+ - levofloxacin interactions in water by fluorescence spectroscopy
EP  - 45
SP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2310
ER  - 

@conference{
author = "Banđur, Dunja and Milenković, Milica and Bartolić, Dragana",
year = "2023",
abstract = "Interactions of antibiotics with biometals can affect their antimicrobial activity by changing their bioavailability, redox properties, and stability toward hydrolysis. [1,2] The pH of biological fluids can significantly affect interactions between biometals and ionizable drugs. In this study, we investigated the interactions of Fe3+ with levofloxacin in water at different pH using fluorescence spectroscopy. The coordination of Fe3+ with levofloxacin is pH dependent due to the ionization of levofloxacin and solubility of Fe3+(aq) ions. The formation of levofloxacin-Fe3+ complex was detected in the pH range 4–5 as a decrease in fluorescence intensity of levofloxacin. The Stern-Volmer diagrams of levofloxacin in the presence of different concentrations of Fe3+ ion at pH 4 and pH 5 correspond to the static fluorescence quenching confirming the coordination of levofloxacin with high-spin Fe3+. At pH < 4 and pH > 5 the addition of FeCl3 as a source of Fe3+ ions results in an increase in fluorescence intensity which can be attributed to the change of the ionic strength of solution and electrostatic interactions between different ionic species instead of coordination of Fe3+ ions with levofloxacin. The coordination of Fe3+ with levofloxacin at pH < 4 is prevented by protonation of its carboxyl and piperazinyl groups (pKa1 = 6.02, pKa2 = 8.15). At pH > 5, despite the presence of zwitterionic and anionic forms of levofloxacin available for coordination, there is no complex formation due to the precipitation of insoluble ferric hydroxide.
References
1. Božić B, Korać J, Stanković DM, Stanić M, Romanović M, Pristov JB, Spasić S, Popović-Bijelić A, Spasojević I, Bajčetić M. Free Radical Biol. Med. 2018, 129, 279.
2. Chen J, Wang Y, Qian Y, Huang T. J. Hazard. Mater. 2017, 335, 117.",
publisher = "Serbian Chemical Society and Serbian Young Chemists’ Club,Karnegijeva 4/Ⅲ, 11000 Belgrade, Serbia",
journal = "9th Conference of Young Chemists of Serbia, Book of Abstracts, 2023,45, CB PP 14",
title = "Investigation of pH dependent Fe3+ - levofloxacin interactions in water by fluorescence spectroscopy",
pages = "45-45",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2310"
}

Banđur, D., Milenković, M.,& Bartolić, D.. (2023). Investigation of pH dependent Fe3+ - levofloxacin interactions in water by fluorescence spectroscopy. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 2023,45, CB PP 14
Serbian Chemical Society and Serbian Young Chemists’ Club,Karnegijeva 4/Ⅲ, 11000 Belgrade, Serbia., 45-45.
https://hdl.handle.net/21.15107/rcub_rimsi_2310

Banđur D, Milenković M, Bartolić D. Investigation of pH dependent Fe3+ - levofloxacin interactions in water by fluorescence spectroscopy. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 2023,45, CB PP 14. 2023;:45-45.
https://hdl.handle.net/21.15107/rcub_rimsi_2310 .

Banđur, Dunja, Milenković, Milica, Bartolić, Dragana, "Investigation of pH dependent Fe3+ - levofloxacin interactions in water by fluorescence spectroscopy" in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 2023,45, CB PP 14 (2023):45-45,
https://hdl.handle.net/21.15107/rcub_rimsi_2310 .

TY  - CONF
AU  - Kalicanin, Nevena
AU  - Balaz, Ana Marija
AU  - Prodanović, Olivera
AU  - Prodanović, Radivoje
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3048
AB  - Opine dehydrogenases are a family of NAD(P)H dependent oxidoreductases, which
catalyze the reductive condensation of an α amino group from an amino acid with an αketo acid during anaerobic glycolysis by regenerating NAD. They are widespread in
cephalopods and mollusks. Opines are associated with crown gall tumor pathogenesis
caused by A. tumefaciens providing nutrients to the pathogen, and novel opine compounds
acting as metallophores have been identified. Besides, opine-type secondary amine
dicarboxylic acids are chiral intermediates of angiotensin-converting enzyme inhibitors. A
novel enzyme originating from an extremophile bacterium, with assumed opine
dehydrogenase function was successfully expressed in Escherichia coli STAR cells and
purified by affinity chromatography. Molecular mass determined by SDS-PAGE was
approximately 40 kDa. The activity was measured by using pyruvate and alanine as
substrates, by which proved that it has opine dehydrogenase activity.
PB  - Faculty of Chemistry, Serbian Biochemical Society
C3  - Proceedings Eleventh Conference, Scientific meeting of an international character "Amazing Biochemistry"
T1  - Production of a novel opine dehydrogenase
SP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3048
ER  - 

@conference{
author = "Kalicanin, Nevena and Balaz, Ana Marija and Prodanović, Olivera and Prodanović, Radivoje",
year = "2022",
abstract = "Opine dehydrogenases are a family of NAD(P)H dependent oxidoreductases, which
catalyze the reductive condensation of an α amino group from an amino acid with an αketo acid during anaerobic glycolysis by regenerating NAD. They are widespread in
cephalopods and mollusks. Opines are associated with crown gall tumor pathogenesis
caused by A. tumefaciens providing nutrients to the pathogen, and novel opine compounds
acting as metallophores have been identified. Besides, opine-type secondary amine
dicarboxylic acids are chiral intermediates of angiotensin-converting enzyme inhibitors. A
novel enzyme originating from an extremophile bacterium, with assumed opine
dehydrogenase function was successfully expressed in Escherichia coli STAR cells and
purified by affinity chromatography. Molecular mass determined by SDS-PAGE was
approximately 40 kDa. The activity was measured by using pyruvate and alanine as
substrates, by which proved that it has opine dehydrogenase activity.",
publisher = "Faculty of Chemistry, Serbian Biochemical Society",
journal = "Proceedings Eleventh Conference, Scientific meeting of an international character "Amazing Biochemistry"",
title = "Production of a novel opine dehydrogenase",
pages = "79",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3048"
}

Kalicanin, N., Balaz, A. M., Prodanović, O.,& Prodanović, R.. (2022). Production of a novel opine dehydrogenase. in Proceedings Eleventh Conference, Scientific meeting of an international character "Amazing Biochemistry"
Faculty of Chemistry, Serbian Biochemical Society., 79.
https://hdl.handle.net/21.15107/rcub_rimsi_3048

Kalicanin N, Balaz AM, Prodanović O, Prodanović R. Production of a novel opine dehydrogenase. in Proceedings Eleventh Conference, Scientific meeting of an international character "Amazing Biochemistry". 2022;:79.
https://hdl.handle.net/21.15107/rcub_rimsi_3048 .

Kalicanin, Nevena, Balaz, Ana Marija, Prodanović, Olivera, Prodanović, Radivoje, "Production of a novel opine dehydrogenase" in Proceedings Eleventh Conference, Scientific meeting of an international character "Amazing Biochemistry" (2022):79,
https://hdl.handle.net/21.15107/rcub_rimsi_3048 .

TY  - JOUR
AU  - Kaličanin, Nevena
AU  - Kovačević, Gordana
AU  - Spasojević, Milica
AU  - Prodanović, Olivera
AU  - Jovanović-Šanta, Suzana
AU  - Škorić, Dušan
AU  - Opsenica, Dejan
AU  - Prodanović, Radivoje
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1768
AB  - The aim of this research was to improve the operational stability and enable the reusability of ω-transaminase for synthesis of new enantiopure chiral amines of steroids. Dihydrotestosterone was used to optimize the synthetic procedure of corresponding amino-steroid on a larger scale. The obtained product 3α-amino-5α-androstan-17β-ol was isolated and characterized. The enzyme was immobilized on a methacrylate-based carrier, giving the specific activity of 1.84 U/g of dry polymer. Higher residual activity of the immobilized enzyme in comparison to the soluble form (100 % versus 35%) after 24 h incubation in 35 % dimethylformamide (DMF) was obtained. The soluble enzyme retained 19 % of the initial activity after 2 h incubation in 35 % DMF at 70 °C, while the activity of the immobilized enzyme decreased only to 75 %. Immobilized retained 85 % of initial activity after ten consecutive cycles of 3α-amino-5α-androstan-17β-ol synthesis. We have tested the specificity of the ArRMut11 variant, further increased its stability by immobilization, and used it in several cycles for the synthesis of 3α-amino-5α-androstan-17β-ol. We showed that the enzyme previously evolved for higher stability as the immobilized variant showed more increased stability and high reusability that can more effectively be applied for the biosynthesis of amino steroids.
PB  - Elsevier
T2  - Process Biochemistry
T1  - Immobilization of ArRMut11 omega-transaminase for increased operational stability and reusability in the synthesis of 3α-amino-5α-androstan-17β-ol
EP  - 680
SP  - 674
VL  - 121
DO  - 10.1016/j.procbio.2022.08.016
ER  - 

@article{
author = "Kaličanin, Nevena and Kovačević, Gordana and Spasojević, Milica and Prodanović, Olivera and Jovanović-Šanta, Suzana and Škorić, Dušan and Opsenica, Dejan and Prodanović, Radivoje",
year = "2022",
abstract = "The aim of this research was to improve the operational stability and enable the reusability of ω-transaminase for synthesis of new enantiopure chiral amines of steroids. Dihydrotestosterone was used to optimize the synthetic procedure of corresponding amino-steroid on a larger scale. The obtained product 3α-amino-5α-androstan-17β-ol was isolated and characterized. The enzyme was immobilized on a methacrylate-based carrier, giving the specific activity of 1.84 U/g of dry polymer. Higher residual activity of the immobilized enzyme in comparison to the soluble form (100 % versus 35%) after 24 h incubation in 35 % dimethylformamide (DMF) was obtained. The soluble enzyme retained 19 % of the initial activity after 2 h incubation in 35 % DMF at 70 °C, while the activity of the immobilized enzyme decreased only to 75 %. Immobilized retained 85 % of initial activity after ten consecutive cycles of 3α-amino-5α-androstan-17β-ol synthesis. We have tested the specificity of the ArRMut11 variant, further increased its stability by immobilization, and used it in several cycles for the synthesis of 3α-amino-5α-androstan-17β-ol. We showed that the enzyme previously evolved for higher stability as the immobilized variant showed more increased stability and high reusability that can more effectively be applied for the biosynthesis of amino steroids.",
publisher = "Elsevier",
journal = "Process Biochemistry",
title = "Immobilization of ArRMut11 omega-transaminase for increased operational stability and reusability in the synthesis of 3α-amino-5α-androstan-17β-ol",
pages = "680-674",
volume = "121",
doi = "10.1016/j.procbio.2022.08.016"
}

Kaličanin, N., Kovačević, G., Spasojević, M., Prodanović, O., Jovanović-Šanta, S., Škorić, D., Opsenica, D.,& Prodanović, R.. (2022). Immobilization of ArRMut11 omega-transaminase for increased operational stability and reusability in the synthesis of 3α-amino-5α-androstan-17β-ol. in Process Biochemistry
Elsevier., 121, 674-680.
https://doi.org/10.1016/j.procbio.2022.08.016

Kaličanin N, Kovačević G, Spasojević M, Prodanović O, Jovanović-Šanta S, Škorić D, Opsenica D, Prodanović R. Immobilization of ArRMut11 omega-transaminase for increased operational stability and reusability in the synthesis of 3α-amino-5α-androstan-17β-ol. in Process Biochemistry. 2022;121:674-680.
doi:10.1016/j.procbio.2022.08.016 .

Kaličanin, Nevena, Kovačević, Gordana, Spasojević, Milica, Prodanović, Olivera, Jovanović-Šanta, Suzana, Škorić, Dušan, Opsenica, Dejan, Prodanović, Radivoje, "Immobilization of ArRMut11 omega-transaminase for increased operational stability and reusability in the synthesis of 3α-amino-5α-androstan-17β-ol" in Process Biochemistry, 121 (2022):674-680,
https://doi.org/10.1016/j.procbio.2022.08.016 . .

TY  - JOUR
AU  - Pantić, Nevena
AU  - Spasojević, Milica
AU  - Stojanović, Zeljko P
AU  - Veljović, Đorđe
AU  - Krstic, Jugoslav
AU  - Balaž, Ana Marija
AU  - Prodanović, Radivoje
AU  - Prodanović, Olivera
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1530
AB  - Novel macroporous copolymers of glycidyl methacrylate and ethylene glycol dimethacrylate with mean pore size diameters ranging from 150 to 310 nm were synthesized by dispersion polymerization and modified with ethylenediamine. The glutaraldehyde and periodate method were employed to immobilize horseradish peroxidase (HRP) onto these carriers. The activity of the immobilized enzyme was greatly affected by the pore size of the carrier. The highest specific activities of 9.65 and 8.94 U/g of dry weight were obtained for HRP immobilized by the periodate-route onto poly(GMA-co-EGDMA) carriers with pore size diameters of 234 and 297 nm, respectively. Stability studies showed an improved operational stability of immobilized peroxidase at 65 degrees C and in an organic solvent. HRP immobilized on a copolymer with a pore size of 234 nm, showing the highest specific activity and good stability, had higher activities at almost all pH values than the native enzyme and the increased K-m value for pyrogallol oxidation. Immobilized HRP retained 80% of its original activity after five consecutive cycles of the pyrogallol oxidation and 98% of its initial activity in a storage stability study. Enzyme immobilized onto the macroporous copolymer with the pore size diameter of 234 nm showed a substantial degree of phenol removal achieved by immobilized peroxidase.
PB  - Springer, New York
T2  - Journal of Polymers and the Environment
T1  - Immobilization of Horseradish Peroxidase on Macroporous Glycidyl-Based Copolymers with Different Surface Characteristics for the Removal of Phenol
DO  - 10.1007/s10924-021-02364-3
ER  - 

@article{
author = "Pantić, Nevena and Spasojević, Milica and Stojanović, Zeljko P and Veljović, Đorđe and Krstic, Jugoslav and Balaž, Ana Marija and Prodanović, Radivoje and Prodanović, Olivera",
year = "2022",
abstract = "Novel macroporous copolymers of glycidyl methacrylate and ethylene glycol dimethacrylate with mean pore size diameters ranging from 150 to 310 nm were synthesized by dispersion polymerization and modified with ethylenediamine. The glutaraldehyde and periodate method were employed to immobilize horseradish peroxidase (HRP) onto these carriers. The activity of the immobilized enzyme was greatly affected by the pore size of the carrier. The highest specific activities of 9.65 and 8.94 U/g of dry weight were obtained for HRP immobilized by the periodate-route onto poly(GMA-co-EGDMA) carriers with pore size diameters of 234 and 297 nm, respectively. Stability studies showed an improved operational stability of immobilized peroxidase at 65 degrees C and in an organic solvent. HRP immobilized on a copolymer with a pore size of 234 nm, showing the highest specific activity and good stability, had higher activities at almost all pH values than the native enzyme and the increased K-m value for pyrogallol oxidation. Immobilized HRP retained 80% of its original activity after five consecutive cycles of the pyrogallol oxidation and 98% of its initial activity in a storage stability study. Enzyme immobilized onto the macroporous copolymer with the pore size diameter of 234 nm showed a substantial degree of phenol removal achieved by immobilized peroxidase.",
publisher = "Springer, New York",
journal = "Journal of Polymers and the Environment",
title = "Immobilization of Horseradish Peroxidase on Macroporous Glycidyl-Based Copolymers with Different Surface Characteristics for the Removal of Phenol",
doi = "10.1007/s10924-021-02364-3"
}

Pantić, N., Spasojević, M., Stojanović, Z. P., Veljović, Đ., Krstic, J., Balaž, A. M., Prodanović, R.,& Prodanović, O.. (2022). Immobilization of Horseradish Peroxidase on Macroporous Glycidyl-Based Copolymers with Different Surface Characteristics for the Removal of Phenol. in Journal of Polymers and the Environment
Springer, New York..
https://doi.org/10.1007/s10924-021-02364-3

Pantić N, Spasojević M, Stojanović ZP, Veljović Đ, Krstic J, Balaž AM, Prodanović R, Prodanović O. Immobilization of Horseradish Peroxidase on Macroporous Glycidyl-Based Copolymers with Different Surface Characteristics for the Removal of Phenol. in Journal of Polymers and the Environment. 2022;.
doi:10.1007/s10924-021-02364-3 .

Pantić, Nevena, Spasojević, Milica, Stojanović, Zeljko P, Veljović, Đorđe, Krstic, Jugoslav, Balaž, Ana Marija, Prodanović, Radivoje, Prodanović, Olivera, "Immobilization of Horseradish Peroxidase on Macroporous Glycidyl-Based Copolymers with Different Surface Characteristics for the Removal of Phenol" in Journal of Polymers and the Environment (2022),
https://doi.org/10.1007/s10924-021-02364-3 . .

TY  - JOUR
AU  - Bartolić, Dragana
AU  - Mojović, Miloš
AU  - Prokopijević, Miloš
AU  - Đikanović, Daniela
AU  - Kalauzi, Aleksandar
AU  - Mutavdžić, Dragosav
AU  - Baosic, Rada
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1544
AB  - BACKGROUND Aflatoxin B-1 (AFB(1)) is the most dangerous of the mycotoxins that contaminate cereal seeds naturally. A stress lignin formation is linked with the accumulation of reactive oxygen species causing a change in the redox status and formation of stable organic radicals, constituting the first layer of defense. The relationship between AFB(1) and changes in lignin organic free radicals in seeds is not known, nor is the part of the seed that is more targeted. Using optical and electron paramagnetic resonance spectroscopy, we investigated AFB(1)-induced changes in lignin and organic free radicals in seeds, and whether the inner and outer seed fractions differ in response to increasing AFB(1). RESULTS Different changes in the content of lignin and free radicals with increasing AFB(1) concentrations were observed in the two seed fractions. There was a significant positive linear correlation (R = 0.9923, P = 0.00005) between lignin content and AFB(1) concentration in the outer fraction, and no correlation between the lignin content and the AFB(1) concentration in the inner fraction. We found a positive correlation between the area of the green spectral emission component (C4) and the AFB(1) concentration in the outer fraction. CONCLUSIONS To the best of our knowledge, the results showed, for the first time, that maize seed fractions respond differently to aflatoxin with regard to their lignin and organic free radical content. Lignin content and (C4) area may be reliable indicators for the screening of lignin changes against AFB(1) content in the seeds, and thus for seed protection capacity.
PB  - Wiley, Hoboken
T2  - Journal of the Science of Food and Agriculture
T1  - Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study
EP  - 2505
IS  - 6
SP  - 2500
VL  - 102
DO  - 10.1002/jsfa.11591
ER  - 

@article{
author = "Bartolić, Dragana and Mojović, Miloš and Prokopijević, Miloš and Đikanović, Daniela and Kalauzi, Aleksandar and Mutavdžić, Dragosav and Baosic, Rada and Radotić, Ksenija",
year = "2022",
abstract = "BACKGROUND Aflatoxin B-1 (AFB(1)) is the most dangerous of the mycotoxins that contaminate cereal seeds naturally. A stress lignin formation is linked with the accumulation of reactive oxygen species causing a change in the redox status and formation of stable organic radicals, constituting the first layer of defense. The relationship between AFB(1) and changes in lignin organic free radicals in seeds is not known, nor is the part of the seed that is more targeted. Using optical and electron paramagnetic resonance spectroscopy, we investigated AFB(1)-induced changes in lignin and organic free radicals in seeds, and whether the inner and outer seed fractions differ in response to increasing AFB(1). RESULTS Different changes in the content of lignin and free radicals with increasing AFB(1) concentrations were observed in the two seed fractions. There was a significant positive linear correlation (R = 0.9923, P = 0.00005) between lignin content and AFB(1) concentration in the outer fraction, and no correlation between the lignin content and the AFB(1) concentration in the inner fraction. We found a positive correlation between the area of the green spectral emission component (C4) and the AFB(1) concentration in the outer fraction. CONCLUSIONS To the best of our knowledge, the results showed, for the first time, that maize seed fractions respond differently to aflatoxin with regard to their lignin and organic free radical content. Lignin content and (C4) area may be reliable indicators for the screening of lignin changes against AFB(1) content in the seeds, and thus for seed protection capacity.",
publisher = "Wiley, Hoboken",
journal = "Journal of the Science of Food and Agriculture",
title = "Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study",
pages = "2505-2500",
number = "6",
volume = "102",
doi = "10.1002/jsfa.11591"
}

Bartolić, D., Mojović, M., Prokopijević, M., Đikanović, D., Kalauzi, A., Mutavdžić, D., Baosic, R.,& Radotić, K.. (2022). Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study. in Journal of the Science of Food and Agriculture
Wiley, Hoboken., 102(6), 2500-2505.
https://doi.org/10.1002/jsfa.11591

Bartolić D, Mojović M, Prokopijević M, Đikanović D, Kalauzi A, Mutavdžić D, Baosic R, Radotić K. Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study. in Journal of the Science of Food and Agriculture. 2022;102(6):2500-2505.
doi:10.1002/jsfa.11591 .

Bartolić, Dragana, Mojović, Miloš, Prokopijević, Miloš, Đikanović, Daniela, Kalauzi, Aleksandar, Mutavdžić, Dragosav, Baosic, Rada, Radotić, Ksenija, "Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study" in Journal of the Science of Food and Agriculture, 102, no. 6 (2022):2500-2505,
https://doi.org/10.1002/jsfa.11591 . .

TY  - JOUR
AU  - Đorđević Aleksić, Jelena
AU  - Kolarević, Stoimir
AU  - Jovanović Marić, Jovana
AU  - Oalđe Pavlović, Mariana
AU  - Sladić, Dušan
AU  - Novaković, Irena
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2018
AB  - Biological activity of 2-tert-butyl-1,4-benzoquinone (TBQ) and its derivatives, 2-tert-butyl-5-(2-propylthio)-1,4-benzoquinone, 2-tert-butyl-5- -(propylthio)-1,4-benzoquinone, 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone, 2-tert-butyl-5-(phenylthio)-1,4-benzoquinone and 2-tert-butyl-6-(phenylthio)- 1,4-benzoquinone, were tested for their antioxidant, antibacterial, toxic, cytotoxic and genotoxic potential. Using the DPPH test, all derivatives showed good antioxidant activity, better than ascorbic acid, and the 2-tert- -butyl-5-(propylthio)-1,4-benzoquinone derivative showed the strongest effect. Better antibacterial potential was observed against Gram-positive bacteria in the broth microdilution method in which the 2-tert-butyl-5-(phenylthio)-1,4- -benzoquinone derivative showed the strongest activity (MIC = 15.6 μM). The results of toxicity tests, using the Brine shrimp test, indicated that the derivatives lose their toxic potential compared to TBQ, except for 2-tert-butyl-6- -(phenylthio)-1,4-benzoquinone, which showed a 3 times stronger effect. Cytotoxicity was assessed by the MTT assay in 24 and 72 h treatments in MRC-5, HS 294T and A549 cell lines in threefold decreasing gradient (11, 33 and 100 μM). Modifications potentiate the cytotoxic effect, and the strongest effect was observed with the 2-tert-butyl-5,6-(ethylendithio)-1,4-benzoquinone derivative. In addition, the genotoxic potential was examined in the MRC-5 cell line using the comet assay. All tested derivatives of TBQ showed a genotoxic effect at all applied subtoxic concentrations. In general, the chemical modifications of TBQ enhanced its biological activity.
PB  - National Library of Serbia
T2  - Journal of the Serbian Chemical Society
T1  - Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone
EP  - 1258
IS  - 11
SP  - 1245
VL  - 87
DO  - 10.2298/JSC220304044D
ER  - 

@article{
author = "Đorđević Aleksić, Jelena and Kolarević, Stoimir and Jovanović Marić, Jovana and Oalđe Pavlović, Mariana and Sladić, Dušan and Novaković, Irena and Vuković-Gačić, Branka",
year = "2022",
abstract = "Biological activity of 2-tert-butyl-1,4-benzoquinone (TBQ) and its derivatives, 2-tert-butyl-5-(2-propylthio)-1,4-benzoquinone, 2-tert-butyl-5- -(propylthio)-1,4-benzoquinone, 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone, 2-tert-butyl-5-(phenylthio)-1,4-benzoquinone and 2-tert-butyl-6-(phenylthio)- 1,4-benzoquinone, were tested for their antioxidant, antibacterial, toxic, cytotoxic and genotoxic potential. Using the DPPH test, all derivatives showed good antioxidant activity, better than ascorbic acid, and the 2-tert- -butyl-5-(propylthio)-1,4-benzoquinone derivative showed the strongest effect. Better antibacterial potential was observed against Gram-positive bacteria in the broth microdilution method in which the 2-tert-butyl-5-(phenylthio)-1,4- -benzoquinone derivative showed the strongest activity (MIC = 15.6 μM). The results of toxicity tests, using the Brine shrimp test, indicated that the derivatives lose their toxic potential compared to TBQ, except for 2-tert-butyl-6- -(phenylthio)-1,4-benzoquinone, which showed a 3 times stronger effect. Cytotoxicity was assessed by the MTT assay in 24 and 72 h treatments in MRC-5, HS 294T and A549 cell lines in threefold decreasing gradient (11, 33 and 100 μM). Modifications potentiate the cytotoxic effect, and the strongest effect was observed with the 2-tert-butyl-5,6-(ethylendithio)-1,4-benzoquinone derivative. In addition, the genotoxic potential was examined in the MRC-5 cell line using the comet assay. All tested derivatives of TBQ showed a genotoxic effect at all applied subtoxic concentrations. In general, the chemical modifications of TBQ enhanced its biological activity.",
publisher = "National Library of Serbia",
journal = "Journal of the Serbian Chemical Society",
title = "Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone",
pages = "1258-1245",
number = "11",
volume = "87",
doi = "10.2298/JSC220304044D"
}

Đorđević Aleksić, J., Kolarević, S., Jovanović Marić, J., Oalđe Pavlović, M., Sladić, D., Novaković, I.,& Vuković-Gačić, B.. (2022). Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone. in Journal of the Serbian Chemical Society
National Library of Serbia., 87(11), 1245-1258.
https://doi.org/10.2298/JSC220304044D

Đorđević Aleksić J, Kolarević S, Jovanović Marić J, Oalđe Pavlović M, Sladić D, Novaković I, Vuković-Gačić B. Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone. in Journal of the Serbian Chemical Society. 2022;87(11):1245-1258.
doi:10.2298/JSC220304044D .

Đorđević Aleksić, Jelena, Kolarević, Stoimir, Jovanović Marić, Jovana, Oalđe Pavlović, Mariana, Sladić, Dušan, Novaković, Irena, Vuković-Gačić, Branka, "Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone" in Journal of the Serbian Chemical Society, 87, no. 11 (2022):1245-1258,
https://doi.org/10.2298/JSC220304044D . .

TY  - CONF
AU  - Tomić, Katarina
AU  - Šokrda Slavić, Marinela
AU  - Kojić, Milan
AU  - Stanisavljević, Nemanja
AU  - Nikolić, Stefan
AU  - Vujčić, Zoran
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1771
AB  - One of the most abundant natural polymers with multidimensional and multifaceted application is starch. Due to energy fuel sustainability concern, the world is focusing on renewable energy including energy from renewable biological materials like starch1. The importance of the enzymatic hydrolysis of granular starch below the temperature of gelatinization has been well recognized, mainly due to energy savings and the effective utilization of biomass, which reduces the overall cost of starch processing2. A new α-amylase gene (Amy35) was cloned from newly isolated thermophilic Anoxybacillus sp. ST4 and expressed in Escherichia coli. The purified recombinant α-amylase had an wide pH optimum range from 4.5 to 8.5 and optimum temperature of 75°C. The enzyme retained 95% of its activity after 3h of incubation at 50 and 60°C. Hydrolysis rates of potato, horseradish and corn starches, at 1% concentration were 20, 70 and 65%, respectively, in a period of 16 h. Analysis of the enzyme properties proved its high efficacy for the digestion of diverse raw starches below gelatinization temperature and, therefore, its potential commercial value for use as an industrial enzyme.
PB  - Faculty of Chemistry, Serbian Biochemical Society
C3  - Serbian Biochemical Society Eleventh Conference
T1  - Cloning and characterization of new raw starch digestion α-amylase from thermophilic Anoxybacillus sp.
SP  - 147
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1771
ER  - 

@conference{
author = "Tomić, Katarina and Šokrda Slavić, Marinela and Kojić, Milan and Stanisavljević, Nemanja and Nikolić, Stefan and Vujčić, Zoran",
year = "2022",
abstract = "One of the most abundant natural polymers with multidimensional and multifaceted application is starch. Due to energy fuel sustainability concern, the world is focusing on renewable energy including energy from renewable biological materials like starch1. The importance of the enzymatic hydrolysis of granular starch below the temperature of gelatinization has been well recognized, mainly due to energy savings and the effective utilization of biomass, which reduces the overall cost of starch processing2. A new α-amylase gene (Amy35) was cloned from newly isolated thermophilic Anoxybacillus sp. ST4 and expressed in Escherichia coli. The purified recombinant α-amylase had an wide pH optimum range from 4.5 to 8.5 and optimum temperature of 75°C. The enzyme retained 95% of its activity after 3h of incubation at 50 and 60°C. Hydrolysis rates of potato, horseradish and corn starches, at 1% concentration were 20, 70 and 65%, respectively, in a period of 16 h. Analysis of the enzyme properties proved its high efficacy for the digestion of diverse raw starches below gelatinization temperature and, therefore, its potential commercial value for use as an industrial enzyme.",
publisher = "Faculty of Chemistry, Serbian Biochemical Society",
journal = "Serbian Biochemical Society Eleventh Conference",
title = "Cloning and characterization of new raw starch digestion α-amylase from thermophilic Anoxybacillus sp.",
pages = "147",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1771"
}

Tomić, K., Šokrda Slavić, M., Kojić, M., Stanisavljević, N., Nikolić, S.,& Vujčić, Z.. (2022). Cloning and characterization of new raw starch digestion α-amylase from thermophilic Anoxybacillus sp.. in Serbian Biochemical Society Eleventh Conference
Faculty of Chemistry, Serbian Biochemical Society., 147.
https://hdl.handle.net/21.15107/rcub_rimsi_1771

Tomić K, Šokrda Slavić M, Kojić M, Stanisavljević N, Nikolić S, Vujčić Z. Cloning and characterization of new raw starch digestion α-amylase from thermophilic Anoxybacillus sp.. in Serbian Biochemical Society Eleventh Conference. 2022;:147.
https://hdl.handle.net/21.15107/rcub_rimsi_1771 .

Tomić, Katarina, Šokrda Slavić, Marinela, Kojić, Milan, Stanisavljević, Nemanja, Nikolić, Stefan, Vujčić, Zoran, "Cloning and characterization of new raw starch digestion α-amylase from thermophilic Anoxybacillus sp." in Serbian Biochemical Society Eleventh Conference (2022):147,
https://hdl.handle.net/21.15107/rcub_rimsi_1771 .

TY  - JOUR
AU  - Popović, Nikolina
AU  - Przulj, Dunja
AU  - Mladenović, Maja
AU  - Prodanović, Olivera
AU  - Ece, Selin
AU  - Ilic-Durdic, Karla
AU  - Ostafe, Raluca
AU  - Fischer, Rainer
AU  - Prodanović, Radivoje
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1490
AB  - High amounts of toxic textile dyes are released into the environment due to coloring and wastewaters treatment processes' inefficiency. To remove dyes from the environment and wastewaters, researchers focused on applying immobilized enzymes due to mild reaction conditions and enzyme nontoxicity. Laccases are oxidases with wide substrate specificity, capable of degradation of many different dye types. Laccase from Streptomyces cyaneus was expressed on the surface of Saccharomyces cerevisiae EBY100 cells. The specific activity of surface-displayed laccase was increased by toluene-induced lysis to 3.1 U/g of cell walls. For cell wall laccase immobilization within hydrogel beads, alginate was modified by dopamine using periodate oxidation and reductive amination and characterized by UV-Vis, FTIR, and NMR spectroscopy. Cell wall laccase was immobilized within alginate and dopamine-alginate beads additionally cross-linked by oxygen and laccase. The immobilized enzyme's specific activity was two times higher using dopamine-alginate compared to native alginate beads, and immobilization yield increased 16 times. Cell wall laccase immobilized within dopamine-alginate beads decolorized Amido Black 10B, Reactive Black 5, Evans Blue, and Remazol Brilliant Blue with 100% efficiency and after ten rounds of multiple-use retained decolorization efficiency of 90% with Evans Blue and 61% with Amido Black.
PB  - Elsevier, Amsterdam
T2  - International Journal of Biological Macromolecules
T1  - Immobilization of yeast cell walls with surface displayed laccase from Streptomyces cyaneus within dopamine-alginate beads for dye decolorization
EP  - 1080
SP  - 1072
VL  - 181
DO  - 10.1016/j.ijbiomac.2021.04.115
ER  - 

@article{
author = "Popović, Nikolina and Przulj, Dunja and Mladenović, Maja and Prodanović, Olivera and Ece, Selin and Ilic-Durdic, Karla and Ostafe, Raluca and Fischer, Rainer and Prodanović, Radivoje",
year = "2021",
abstract = "High amounts of toxic textile dyes are released into the environment due to coloring and wastewaters treatment processes' inefficiency. To remove dyes from the environment and wastewaters, researchers focused on applying immobilized enzymes due to mild reaction conditions and enzyme nontoxicity. Laccases are oxidases with wide substrate specificity, capable of degradation of many different dye types. Laccase from Streptomyces cyaneus was expressed on the surface of Saccharomyces cerevisiae EBY100 cells. The specific activity of surface-displayed laccase was increased by toluene-induced lysis to 3.1 U/g of cell walls. For cell wall laccase immobilization within hydrogel beads, alginate was modified by dopamine using periodate oxidation and reductive amination and characterized by UV-Vis, FTIR, and NMR spectroscopy. Cell wall laccase was immobilized within alginate and dopamine-alginate beads additionally cross-linked by oxygen and laccase. The immobilized enzyme's specific activity was two times higher using dopamine-alginate compared to native alginate beads, and immobilization yield increased 16 times. Cell wall laccase immobilized within dopamine-alginate beads decolorized Amido Black 10B, Reactive Black 5, Evans Blue, and Remazol Brilliant Blue with 100% efficiency and after ten rounds of multiple-use retained decolorization efficiency of 90% with Evans Blue and 61% with Amido Black.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Immobilization of yeast cell walls with surface displayed laccase from Streptomyces cyaneus within dopamine-alginate beads for dye decolorization",
pages = "1080-1072",
volume = "181",
doi = "10.1016/j.ijbiomac.2021.04.115"
}

Popović, N., Przulj, D., Mladenović, M., Prodanović, O., Ece, S., Ilic-Durdic, K., Ostafe, R., Fischer, R.,& Prodanović, R.. (2021). Immobilization of yeast cell walls with surface displayed laccase from Streptomyces cyaneus within dopamine-alginate beads for dye decolorization. in International Journal of Biological Macromolecules
Elsevier, Amsterdam., 181, 1072-1080.
https://doi.org/10.1016/j.ijbiomac.2021.04.115

Popović N, Przulj D, Mladenović M, Prodanović O, Ece S, Ilic-Durdic K, Ostafe R, Fischer R, Prodanović R. Immobilization of yeast cell walls with surface displayed laccase from Streptomyces cyaneus within dopamine-alginate beads for dye decolorization. in International Journal of Biological Macromolecules. 2021;181:1072-1080.
doi:10.1016/j.ijbiomac.2021.04.115 .

Popović, Nikolina, Przulj, Dunja, Mladenović, Maja, Prodanović, Olivera, Ece, Selin, Ilic-Durdic, Karla, Ostafe, Raluca, Fischer, Rainer, Prodanović, Radivoje, "Immobilization of yeast cell walls with surface displayed laccase from Streptomyces cyaneus within dopamine-alginate beads for dye decolorization" in International Journal of Biological Macromolecules, 181 (2021):1072-1080,
https://doi.org/10.1016/j.ijbiomac.2021.04.115 . .

TY  - JOUR
AU  - Popović, Nikolina
AU  - Stanisic, Marija
AU  - Ilic-Durdic, Karla
AU  - Prodanović, Olivera
AU  - Polović, Natalija
AU  - Prodanović, Radivoje
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1484
AB  - Pectins are a group of heterologous polysaccharides capable of forming hydrogels and applicable in many industrial processes. A new type of modified pectin was synthesized by periodate oxidation and reductive amination with dopamine and sodium cyanoborohydride. The success of modification was confirmed by UV-Vis, FTIR, and H-1 NMR spectroscopy. The obtained dopamine-pectin could form hydrogels by ionic crosslinking of carboxyl groups with calcium or by crosslinking phenol groups with laccase. For enzymatic crosslinking with laccase from Streptomyces cyaneus expressed in E. coli, isolation and purification of the enzyme was done. Using emulsion-based enzymatic crosslinking polymerization, dopamine-pectin microbeads with immobilized laccase were made. The immobilized laccase showed improved thermal and pH stability in comparison to the free enzyme. The immobilized biocatalyst effectively decolorized various dyes: Amido Black 10B, Reactive Black 5, and Evans Blue. After ten cycles of repeated use, the microbead immobilized laccase could still decolorize 60% and 36% of Amido Black 10B and Reactive Black 5, respectively.
PB  - Elsevier, Amsterdam
T2  - Environmental Technology & Innovation
T1  - Dopamine-modified pectin for a Streptomyces cyaneus laccase induced microbeads formation, immobilization, and textile dyes decolorization
VL  - 22
DO  - 10.1016/j.eti.2021.101399
ER  - 

@article{
author = "Popović, Nikolina and Stanisic, Marija and Ilic-Durdic, Karla and Prodanović, Olivera and Polović, Natalija and Prodanović, Radivoje",
year = "2021",
abstract = "Pectins are a group of heterologous polysaccharides capable of forming hydrogels and applicable in many industrial processes. A new type of modified pectin was synthesized by periodate oxidation and reductive amination with dopamine and sodium cyanoborohydride. The success of modification was confirmed by UV-Vis, FTIR, and H-1 NMR spectroscopy. The obtained dopamine-pectin could form hydrogels by ionic crosslinking of carboxyl groups with calcium or by crosslinking phenol groups with laccase. For enzymatic crosslinking with laccase from Streptomyces cyaneus expressed in E. coli, isolation and purification of the enzyme was done. Using emulsion-based enzymatic crosslinking polymerization, dopamine-pectin microbeads with immobilized laccase were made. The immobilized laccase showed improved thermal and pH stability in comparison to the free enzyme. The immobilized biocatalyst effectively decolorized various dyes: Amido Black 10B, Reactive Black 5, and Evans Blue. After ten cycles of repeated use, the microbead immobilized laccase could still decolorize 60% and 36% of Amido Black 10B and Reactive Black 5, respectively.",
publisher = "Elsevier, Amsterdam",
journal = "Environmental Technology & Innovation",
title = "Dopamine-modified pectin for a Streptomyces cyaneus laccase induced microbeads formation, immobilization, and textile dyes decolorization",
volume = "22",
doi = "10.1016/j.eti.2021.101399"
}

Popović, N., Stanisic, M., Ilic-Durdic, K., Prodanović, O., Polović, N.,& Prodanović, R.. (2021). Dopamine-modified pectin for a Streptomyces cyaneus laccase induced microbeads formation, immobilization, and textile dyes decolorization. in Environmental Technology & Innovation
Elsevier, Amsterdam., 22.
https://doi.org/10.1016/j.eti.2021.101399

Popović N, Stanisic M, Ilic-Durdic K, Prodanović O, Polović N, Prodanović R. Dopamine-modified pectin for a Streptomyces cyaneus laccase induced microbeads formation, immobilization, and textile dyes decolorization. in Environmental Technology & Innovation. 2021;22.
doi:10.1016/j.eti.2021.101399 .

Popović, Nikolina, Stanisic, Marija, Ilic-Durdic, Karla, Prodanović, Olivera, Polović, Natalija, Prodanović, Radivoje, "Dopamine-modified pectin for a Streptomyces cyaneus laccase induced microbeads formation, immobilization, and textile dyes decolorization" in Environmental Technology & Innovation, 22 (2021),
https://doi.org/10.1016/j.eti.2021.101399 . .

TY  - JOUR
AU  - Pantić, Nevena
AU  - Prodanović, Radivoje
AU  - Ilic-Durdic, Karla
AU  - Polović, Natalija
AU  - Spasojević, Milica
AU  - Prodanović, Olivera
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1476
AB  - Removal of phenolic compounds from water is of major interest over the years, since they are one of the most common pollutants in aqueous systems. Horseradish peroxidase (HRP) is the most investigated biocatalyst for this purpose. Inactivation of the enzyme is a major issue which can be successfully overcome by the enzyme immobilization on different polymers. In this study, tyramine-alginate micro-beads were used as carriers for the immobilization of horseradish peroxidase. The effect of the oxidation degree of tyramine-alginates on a specific activity of the enzyme was tested. An increase in the concentration of oxidized alginate from 2.5 to 20% resulted in a gradual increase in the specific activity from 0.05 to 0.67 U/mL. HRP immobilized within these microbeads was tested for the phenol removal in a batch reactor. Reaction conditions were optimized to achieve a high removal efficiency and substantial reusability of the system. In this study, for the first time, an internal generation of hydrogen peroxide from glucose and glucose oxidase was employed in the phenol removal process with HRP immobilized on tyramine-alginate. Within 6 h of repeated use 96% of phenol was removed when the system for internal delivery of H2O2, composed of 0.187 U/mL of glucose oxidase and 4 mmol/L of glucose was employed. A common straightforward addition of hydrogen peroxide provided the removal efficiency of only 42%, under the same reaction conditions. The highest efficiency of the phenol removal (96%) was obtained with HRP immobilized within 20 mol% oxidized tyramine-alginate microbeads. Fifteen mol% oxidized tyramine-alginate showed lower removal efficiency in the first cycle of use (73%) but more promising reusability, since the immobilized enzyme retained 61% of its initial activity even after four consecutive cycles of use.
PB  - Elsevier, Amsterdam
T2  - Environmental Technology & Innovation
T1  - Optimization of phenol removal with horseradish peroxidase encapsulated within tyramine-alginate micro-beads
VL  - 21
DO  - 10.1016/j.eti.2020.101211
ER  - 

@article{
author = "Pantić, Nevena and Prodanović, Radivoje and Ilic-Durdic, Karla and Polović, Natalija and Spasojević, Milica and Prodanović, Olivera",
year = "2021",
abstract = "Removal of phenolic compounds from water is of major interest over the years, since they are one of the most common pollutants in aqueous systems. Horseradish peroxidase (HRP) is the most investigated biocatalyst for this purpose. Inactivation of the enzyme is a major issue which can be successfully overcome by the enzyme immobilization on different polymers. In this study, tyramine-alginate micro-beads were used as carriers for the immobilization of horseradish peroxidase. The effect of the oxidation degree of tyramine-alginates on a specific activity of the enzyme was tested. An increase in the concentration of oxidized alginate from 2.5 to 20% resulted in a gradual increase in the specific activity from 0.05 to 0.67 U/mL. HRP immobilized within these microbeads was tested for the phenol removal in a batch reactor. Reaction conditions were optimized to achieve a high removal efficiency and substantial reusability of the system. In this study, for the first time, an internal generation of hydrogen peroxide from glucose and glucose oxidase was employed in the phenol removal process with HRP immobilized on tyramine-alginate. Within 6 h of repeated use 96% of phenol was removed when the system for internal delivery of H2O2, composed of 0.187 U/mL of glucose oxidase and 4 mmol/L of glucose was employed. A common straightforward addition of hydrogen peroxide provided the removal efficiency of only 42%, under the same reaction conditions. The highest efficiency of the phenol removal (96%) was obtained with HRP immobilized within 20 mol% oxidized tyramine-alginate microbeads. Fifteen mol% oxidized tyramine-alginate showed lower removal efficiency in the first cycle of use (73%) but more promising reusability, since the immobilized enzyme retained 61% of its initial activity even after four consecutive cycles of use.",
publisher = "Elsevier, Amsterdam",
journal = "Environmental Technology & Innovation",
title = "Optimization of phenol removal with horseradish peroxidase encapsulated within tyramine-alginate micro-beads",
volume = "21",
doi = "10.1016/j.eti.2020.101211"
}

Pantić, N., Prodanović, R., Ilic-Durdic, K., Polović, N., Spasojević, M.,& Prodanović, O.. (2021). Optimization of phenol removal with horseradish peroxidase encapsulated within tyramine-alginate micro-beads. in Environmental Technology & Innovation
Elsevier, Amsterdam., 21.
https://doi.org/10.1016/j.eti.2020.101211

Pantić N, Prodanović R, Ilic-Durdic K, Polović N, Spasojević M, Prodanović O. Optimization of phenol removal with horseradish peroxidase encapsulated within tyramine-alginate micro-beads. in Environmental Technology & Innovation. 2021;21.
doi:10.1016/j.eti.2020.101211 .

Pantić, Nevena, Prodanović, Radivoje, Ilic-Durdic, Karla, Polović, Natalija, Spasojević, Milica, Prodanović, Olivera, "Optimization of phenol removal with horseradish peroxidase encapsulated within tyramine-alginate micro-beads" in Environmental Technology & Innovation, 21 (2021),
https://doi.org/10.1016/j.eti.2020.101211 . .

TY  - JOUR
AU  - Jovanović, Jelena
AU  - Ćirković, Jovana
AU  - Radojković, Aleksandar
AU  - Mutavdžić, Dragosav
AU  - Tanasijević, Gordana
AU  - Joksimović, Kristina
AU  - Bakic, Gordana
AU  - Branković, Goran
AU  - Branković, Zorica
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1422
AB  - This investigation was aimed to develop films and coatings based on natural biopolymers and active components, with physicochemical and functional properties for application in antimicrobial packaging. Biopolymer films were obtained from emulsions and dispersions by casting method. Coatings were formed by the application of emulsions on existing packaging. Synthesis of emulsions and dispersions were performed by combining biopolymers (chitosan-gelatin, pectin-gelatin) with lemongrass essential oil (LEO), Zn(CH3COO)2.2H2O, or ZnO, as active components. Fourier transform infrared (FTIR) spectra of the films showed that the addition of glycerol contributed to the forming of strong hydrogen bonds between glycerol and chitosan-gelatin or pectin-gelatin composites, facilitating the process of polymer branching, which is substantial for encapsulation. Thermal analyses revealed two characteristic processes occurring at 110 degrees C (water evaporation) and within 215-235 degrees C (degradation of polysaccharide and glycerol). Major weight losses were observed at 290 degrees C (chitosan-gelatin films) and 215 degrees C (pectin-gelatin films). The chitosan-gelatin films exhibited a lower degree of solubility (31-21%), and better mechanical properties comparing with the pectin-gelatin films. The chitosan-gelatin emulsions and dispersions exhibited a higher antibacterial effect in vitro against E. coli, B. subtilis, and S. aureus. In the case of the pectin-gelatin emulsions, the impact of LEO on the antibacterial activity was evident. The effects of biopolymer coatings on the development of microorganisms on fresh raspberries (Rubus idaeus L.) were performed in vivo during eight days of raspberry storage at refrigerator temperature. The tested coatings extended the shelf life of stored raspberries from four to eight days. The synergistic effect between LEO and ZnO or Zn-Ac was observed both in vivo and in vitro.
PB  - Elsevier Science Sa, Lausanne
T2  - Progress in Organic Coatings
T1  - Chitosan and pectin-based films and coatings with active components for application in antimicrobial food packaging
VL  - 158
DO  - 10.1016/j.porgcoat.2021.106349
ER  - 

@article{
author = "Jovanović, Jelena and Ćirković, Jovana and Radojković, Aleksandar and Mutavdžić, Dragosav and Tanasijević, Gordana and Joksimović, Kristina and Bakic, Gordana and Branković, Goran and Branković, Zorica",
year = "2021",
abstract = "This investigation was aimed to develop films and coatings based on natural biopolymers and active components, with physicochemical and functional properties for application in antimicrobial packaging. Biopolymer films were obtained from emulsions and dispersions by casting method. Coatings were formed by the application of emulsions on existing packaging. Synthesis of emulsions and dispersions were performed by combining biopolymers (chitosan-gelatin, pectin-gelatin) with lemongrass essential oil (LEO), Zn(CH3COO)2.2H2O, or ZnO, as active components. Fourier transform infrared (FTIR) spectra of the films showed that the addition of glycerol contributed to the forming of strong hydrogen bonds between glycerol and chitosan-gelatin or pectin-gelatin composites, facilitating the process of polymer branching, which is substantial for encapsulation. Thermal analyses revealed two characteristic processes occurring at 110 degrees C (water evaporation) and within 215-235 degrees C (degradation of polysaccharide and glycerol). Major weight losses were observed at 290 degrees C (chitosan-gelatin films) and 215 degrees C (pectin-gelatin films). The chitosan-gelatin films exhibited a lower degree of solubility (31-21%), and better mechanical properties comparing with the pectin-gelatin films. The chitosan-gelatin emulsions and dispersions exhibited a higher antibacterial effect in vitro against E. coli, B. subtilis, and S. aureus. In the case of the pectin-gelatin emulsions, the impact of LEO on the antibacterial activity was evident. The effects of biopolymer coatings on the development of microorganisms on fresh raspberries (Rubus idaeus L.) were performed in vivo during eight days of raspberry storage at refrigerator temperature. The tested coatings extended the shelf life of stored raspberries from four to eight days. The synergistic effect between LEO and ZnO or Zn-Ac was observed both in vivo and in vitro.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Progress in Organic Coatings",
title = "Chitosan and pectin-based films and coatings with active components for application in antimicrobial food packaging",
volume = "158",
doi = "10.1016/j.porgcoat.2021.106349"
}

Jovanović, J., Ćirković, J., Radojković, A., Mutavdžić, D., Tanasijević, G., Joksimović, K., Bakic, G., Branković, G.,& Branković, Z.. (2021). Chitosan and pectin-based films and coatings with active components for application in antimicrobial food packaging. in Progress in Organic Coatings
Elsevier Science Sa, Lausanne., 158.
https://doi.org/10.1016/j.porgcoat.2021.106349

Jovanović J, Ćirković J, Radojković A, Mutavdžić D, Tanasijević G, Joksimović K, Bakic G, Branković G, Branković Z. Chitosan and pectin-based films and coatings with active components for application in antimicrobial food packaging. in Progress in Organic Coatings. 2021;158.
doi:10.1016/j.porgcoat.2021.106349 .

Jovanović, Jelena, Ćirković, Jovana, Radojković, Aleksandar, Mutavdžić, Dragosav, Tanasijević, Gordana, Joksimović, Kristina, Bakic, Gordana, Branković, Goran, Branković, Zorica, "Chitosan and pectin-based films and coatings with active components for application in antimicrobial food packaging" in Progress in Organic Coatings, 158 (2021),
https://doi.org/10.1016/j.porgcoat.2021.106349 . .

TY  - JOUR
AU  - Milenković, Ivana
AU  - Radotić, Ksenija
AU  - Trifković, Jelena
AU  - Vujisic, Ljubodrag V
AU  - Beskoski, Vladimir P.
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1456
AB  - Literature data about semi-volatile organic compounds in plants and the effect of cerium oxide nanoparticles on them are scarce. Surface modification of nanoparticles may change nanoparticle-environment interaction, and therefore affects compounds in plants. In this research, uncoated and glucose-, levan-, and pullulan-coated cerium oxide nanoparticles were used for wheat and pea treatment during the growth. The aim was the screening of semi-volatile organic compounds from plants' shoots using comprehensive two-dimensional gas chromatography-mass spectrometry, a powerful separation technique allowing to reach unique separation resolution, and investigation of qualitative changes after the treatmentwith coated cerium oxide nanoparticles. The resultswere analyzed by the identification of individual peaks and fingerprint analysis by image processing. Wheat samples contained a higher number of semi-volatile organic compounds (108) compared to pea (77) but were less affected by the treatments with coated nanoparticles. The highest number of compounds was detected in wheat after the treatment with levan- and pullulan-coated nanoparticles, and in pea after treatment with levan-coated nanoparticles. This article reports a successful application of a semi-volatile organic compounds profile presented only as categorical variables and unique fingerprint images for the inter-cultivar recognition. This method may be useful in screening nanoparticles' effects on different plants.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Journal of Separation Science
T1  - Screening of semi-volatile compounds in plants treated with coated cerium oxide nanoparticles by comprehensive two-dimensional gas chromatography
EP  - 2268
IS  - 11
SP  - 2260
VL  - 44
DO  - 10.1002/jssc.202100145
ER  - 

@article{
author = "Milenković, Ivana and Radotić, Ksenija and Trifković, Jelena and Vujisic, Ljubodrag V and Beskoski, Vladimir P.",
year = "2021",
abstract = "Literature data about semi-volatile organic compounds in plants and the effect of cerium oxide nanoparticles on them are scarce. Surface modification of nanoparticles may change nanoparticle-environment interaction, and therefore affects compounds in plants. In this research, uncoated and glucose-, levan-, and pullulan-coated cerium oxide nanoparticles were used for wheat and pea treatment during the growth. The aim was the screening of semi-volatile organic compounds from plants' shoots using comprehensive two-dimensional gas chromatography-mass spectrometry, a powerful separation technique allowing to reach unique separation resolution, and investigation of qualitative changes after the treatmentwith coated cerium oxide nanoparticles. The resultswere analyzed by the identification of individual peaks and fingerprint analysis by image processing. Wheat samples contained a higher number of semi-volatile organic compounds (108) compared to pea (77) but were less affected by the treatments with coated nanoparticles. The highest number of compounds was detected in wheat after the treatment with levan- and pullulan-coated nanoparticles, and in pea after treatment with levan-coated nanoparticles. This article reports a successful application of a semi-volatile organic compounds profile presented only as categorical variables and unique fingerprint images for the inter-cultivar recognition. This method may be useful in screening nanoparticles' effects on different plants.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Journal of Separation Science",
title = "Screening of semi-volatile compounds in plants treated with coated cerium oxide nanoparticles by comprehensive two-dimensional gas chromatography",
pages = "2268-2260",
number = "11",
volume = "44",
doi = "10.1002/jssc.202100145"
}

Milenković, I., Radotić, K., Trifković, J., Vujisic, L. V.,& Beskoski, V. P.. (2021). Screening of semi-volatile compounds in plants treated with coated cerium oxide nanoparticles by comprehensive two-dimensional gas chromatography. in Journal of Separation Science
Wiley-V C H Verlag Gmbh, Weinheim., 44(11), 2260-2268.
https://doi.org/10.1002/jssc.202100145

Milenković I, Radotić K, Trifković J, Vujisic LV, Beskoski VP. Screening of semi-volatile compounds in plants treated with coated cerium oxide nanoparticles by comprehensive two-dimensional gas chromatography. in Journal of Separation Science. 2021;44(11):2260-2268.
doi:10.1002/jssc.202100145 .

Milenković, Ivana, Radotić, Ksenija, Trifković, Jelena, Vujisic, Ljubodrag V, Beskoski, Vladimir P., "Screening of semi-volatile compounds in plants treated with coated cerium oxide nanoparticles by comprehensive two-dimensional gas chromatography" in Journal of Separation Science, 44, no. 11 (2021):2260-2268,
https://doi.org/10.1002/jssc.202100145 . .

TY  - JOUR
AU  - Milenković, Ivana
AU  - Radotić, Ksenija
AU  - Despotović, Jovana
AU  - Loncarević, Branka
AU  - Ljesević, Marija
AU  - Spasić, Slađana
AU  - Nikolić, Aleksandra
AU  - Beskoski, Vladimir P.
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1423
AB  - Cerium oxide nanoparticles (nCeO(2)) have widespread applications, but they can be hazardous to the environment. Some reports indicate the toxic effect of nCeO(2) on tested animals, but literature data are mainly contradictory. Coating of nCeO(2) can improve their suspension stability and change their interaction with the environment, which can consequently decrease their toxic effects. Herein, the exopolysaccharides levan and pullulan, due to their high water solubility, biocompatibility, and ability to form film, were used to coat nCeO(2). Additionally, the monosaccharide glucose was used, since it is a common material for nanoparticle coating. This is the first study investigating the impact of carbohydrate-coated nCeO(2) in comparison to uncoated nCeO(2) using different model organisms. The aim of this study was to test the acute toxicity of carbohydrate-coated nCeO(2) on the bacterium Vibrio fischeri NRRL B-11177, the crustacean Daphnia magna, and zebrafish Danio rerio. The second aim was to investigate the effects of nCeO(2) on respiration in Daphnia magna which was performed for the first time. Finally, it was important to see the relation between Ce bioaccumulation in Daphnia magna and Danio rerio and other investigated parameters. Our results revealed that the coating decreased the toxicity of nCeO(2) on Vibrio fischeri. The coating of nCeO(2) did not affect the nanoparticles' accumulation/adsorption or mortality in Daphnia magna or Danio rerio. Monitoring of respiration in Daphnia magna revealed changes in CO2 production after exposure to coated nCeO(2), while the crustacean's O-2 consumption was not affected by any of the coated nCeO(2). In summary, this study revealed that, at 200 mg L-1 uncoated and carbohydrate-coated nCeO(2) are not toxic for the tested organisms, however, the CO2 production in Daphnia magna is different when they are treated with coated and uncoated nCeO(2). The highest production was in glucose and levan-coated nCeO(2) according to their highest suspension stability. Daphnia magna (D. magna), Danio rerio (D. rerio), Vibrio fischeri (V. fischeri)
PB  - Elsevier, Amsterdam
T2  - Aquatic Toxicology
T1  - Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio
VL  - 236
DO  - 10.1016/j.aquatox.2021.105867
ER  - 

@article{
author = "Milenković, Ivana and Radotić, Ksenija and Despotović, Jovana and Loncarević, Branka and Ljesević, Marija and Spasić, Slađana and Nikolić, Aleksandra and Beskoski, Vladimir P.",
year = "2021",
abstract = "Cerium oxide nanoparticles (nCeO(2)) have widespread applications, but they can be hazardous to the environment. Some reports indicate the toxic effect of nCeO(2) on tested animals, but literature data are mainly contradictory. Coating of nCeO(2) can improve their suspension stability and change their interaction with the environment, which can consequently decrease their toxic effects. Herein, the exopolysaccharides levan and pullulan, due to their high water solubility, biocompatibility, and ability to form film, were used to coat nCeO(2). Additionally, the monosaccharide glucose was used, since it is a common material for nanoparticle coating. This is the first study investigating the impact of carbohydrate-coated nCeO(2) in comparison to uncoated nCeO(2) using different model organisms. The aim of this study was to test the acute toxicity of carbohydrate-coated nCeO(2) on the bacterium Vibrio fischeri NRRL B-11177, the crustacean Daphnia magna, and zebrafish Danio rerio. The second aim was to investigate the effects of nCeO(2) on respiration in Daphnia magna which was performed for the first time. Finally, it was important to see the relation between Ce bioaccumulation in Daphnia magna and Danio rerio and other investigated parameters. Our results revealed that the coating decreased the toxicity of nCeO(2) on Vibrio fischeri. The coating of nCeO(2) did not affect the nanoparticles' accumulation/adsorption or mortality in Daphnia magna or Danio rerio. Monitoring of respiration in Daphnia magna revealed changes in CO2 production after exposure to coated nCeO(2), while the crustacean's O-2 consumption was not affected by any of the coated nCeO(2). In summary, this study revealed that, at 200 mg L-1 uncoated and carbohydrate-coated nCeO(2) are not toxic for the tested organisms, however, the CO2 production in Daphnia magna is different when they are treated with coated and uncoated nCeO(2). The highest production was in glucose and levan-coated nCeO(2) according to their highest suspension stability. Daphnia magna (D. magna), Danio rerio (D. rerio), Vibrio fischeri (V. fischeri)",
publisher = "Elsevier, Amsterdam",
journal = "Aquatic Toxicology",
title = "Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio",
volume = "236",
doi = "10.1016/j.aquatox.2021.105867"
}

Milenković, I., Radotić, K., Despotović, J., Loncarević, B., Ljesević, M., Spasić, S., Nikolić, A.,& Beskoski, V. P.. (2021). Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio. in Aquatic Toxicology
Elsevier, Amsterdam., 236.
https://doi.org/10.1016/j.aquatox.2021.105867

Milenković I, Radotić K, Despotović J, Loncarević B, Ljesević M, Spasić S, Nikolić A, Beskoski VP. Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio. in Aquatic Toxicology. 2021;236.
doi:10.1016/j.aquatox.2021.105867 .

Milenković, Ivana, Radotić, Ksenija, Despotović, Jovana, Loncarević, Branka, Ljesević, Marija, Spasić, Slađana, Nikolić, Aleksandra, Beskoski, Vladimir P., "Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio" in Aquatic Toxicology, 236 (2021),
https://doi.org/10.1016/j.aquatox.2021.105867 . .

TY  - JOUR
AU  - Ristic, Predrag
AU  - Filipović, Nenad
AU  - Blagojević, Vladimir
AU  - Ćirković, Jovana
AU  - Hollo, Berta Barta
AU  - Dokic, Veljko R.
AU  - Donnard, Morgan
AU  - Gulea, Mihaela
AU  - Marjanović, Ivana
AU  - Klisuric, Olivera R.
AU  - Todorović, Tamara R.
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1461
AB  - Four silver-based coordination polymers, {[Ag(L1)(2)]NO3}(infinity) (1), {[Ag(L1)(2)]ClO4}(infinity) (2), {[Ag(L2)(2)]NO3 center dot H2O}(infinity) (3) and {[Ag(L2)(2)]ClO4}(infinity) (4), were synthesized using the thiomorpholine-4-carbonitrile (L1) and piperazine-1,4-dicarbonitrile (L2) ligands. Compounds 1 and 2 are two-dimensional, while 3 and 4 are three-dimensional. L1 and L2 are 1,4-bis-monodentate ligands in all compounds, while Ag(i) ions are four-coordinated in a slightly distorted tetrahedral geometry. Topological analysis in standard representations suggests that underlying nets in 1 and 2 have an sql topology, while 3 and 4 exhibit a dia topology. Thermal analysis shows that 3 loses crystalline water at room temperature, while other compounds show good thermal stability. All compounds show good photocatalytic activity for photocatalytic degradation of the mordant blue 9 dye, with reaction rates in the range 0.029 to 0.061 min(-1). The best result was obtained for compound 4, which can be correlated to its largest lattice volume.
PB  - Royal Soc Chemistry, Cambridge
T2  - Crystengcomm
T1  - 2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior
EP  - 4815
IS  - 27
SP  - 4799
VL  - 23
DO  - 10.1039/d1ce00394a
ER  - 

@article{
author = "Ristic, Predrag and Filipović, Nenad and Blagojević, Vladimir and Ćirković, Jovana and Hollo, Berta Barta and Dokic, Veljko R. and Donnard, Morgan and Gulea, Mihaela and Marjanović, Ivana and Klisuric, Olivera R. and Todorović, Tamara R.",
year = "2021",
abstract = "Four silver-based coordination polymers, {[Ag(L1)(2)]NO3}(infinity) (1), {[Ag(L1)(2)]ClO4}(infinity) (2), {[Ag(L2)(2)]NO3 center dot H2O}(infinity) (3) and {[Ag(L2)(2)]ClO4}(infinity) (4), were synthesized using the thiomorpholine-4-carbonitrile (L1) and piperazine-1,4-dicarbonitrile (L2) ligands. Compounds 1 and 2 are two-dimensional, while 3 and 4 are three-dimensional. L1 and L2 are 1,4-bis-monodentate ligands in all compounds, while Ag(i) ions are four-coordinated in a slightly distorted tetrahedral geometry. Topological analysis in standard representations suggests that underlying nets in 1 and 2 have an sql topology, while 3 and 4 exhibit a dia topology. Thermal analysis shows that 3 loses crystalline water at room temperature, while other compounds show good thermal stability. All compounds show good photocatalytic activity for photocatalytic degradation of the mordant blue 9 dye, with reaction rates in the range 0.029 to 0.061 min(-1). The best result was obtained for compound 4, which can be correlated to its largest lattice volume.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Crystengcomm",
title = "2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior",
pages = "4815-4799",
number = "27",
volume = "23",
doi = "10.1039/d1ce00394a"
}

Ristic, P., Filipović, N., Blagojević, V., Ćirković, J., Hollo, B. B., Dokic, V. R., Donnard, M., Gulea, M., Marjanović, I., Klisuric, O. R.,& Todorović, T. R.. (2021). 2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior. in Crystengcomm
Royal Soc Chemistry, Cambridge., 23(27), 4799-4815.
https://doi.org/10.1039/d1ce00394a

Ristic P, Filipović N, Blagojević V, Ćirković J, Hollo BB, Dokic VR, Donnard M, Gulea M, Marjanović I, Klisuric OR, Todorović TR. 2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior. in Crystengcomm. 2021;23(27):4799-4815.
doi:10.1039/d1ce00394a .

Ristic, Predrag, Filipović, Nenad, Blagojević, Vladimir, Ćirković, Jovana, Hollo, Berta Barta, Dokic, Veljko R., Donnard, Morgan, Gulea, Mihaela, Marjanović, Ivana, Klisuric, Olivera R., Todorović, Tamara R., "2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior" in Crystengcomm, 23, no. 27 (2021):4799-4815,
https://doi.org/10.1039/d1ce00394a . .

TY  - JOUR
AU  - Milosavljević, Sasa
AU  - Jadranin, Milka
AU  - Mladenović, Mica
AU  - Tešević, Vele
AU  - Menković, Nebojša
AU  - Mutavdžić, Dragosav
AU  - Krstic, Gordana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1478
AB  - The aim of this research was to verify the authenticity of monofloral honeys from the territory of the Republic of Serbia on the basis of physicochemical parameters routinely measured in honey quality control using multivariate analysis. Seventeen samples of monofloral honey (11 samples of acacia honey and 6 samples of sunflower honey) from the territory of the Republic of Serbia were analyzed. Physicochemical analysis of the samples included the examination of basic quality parameters and qualitative and quantitative analyses of phenolic compounds. In the samples tested, a total of 93 phenolic compounds were tentatively identified, and 19 of them were quantified. The obtained physicochemical analysis of the data served as input for the multivariate analysis. The h..t map, which is useful for visualizing numerical data, was used for this purpose. The obtained results showed that the applied data can serve to clearly separate acacia and sunflower honeys.
PB  - Soc Chemists Technologists Madeconia, Skopje
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - Physicochemical parameters as indicators of the authenticity of monofloral honey from the territory of the republic of Serbia
EP  - 67
IS  - 1
SP  - 57
VL  - 40
DO  - 10.20450/mjcce.2021.2186
ER  - 

@article{
author = "Milosavljević, Sasa and Jadranin, Milka and Mladenović, Mica and Tešević, Vele and Menković, Nebojša and Mutavdžić, Dragosav and Krstic, Gordana",
year = "2021",
abstract = "The aim of this research was to verify the authenticity of monofloral honeys from the territory of the Republic of Serbia on the basis of physicochemical parameters routinely measured in honey quality control using multivariate analysis. Seventeen samples of monofloral honey (11 samples of acacia honey and 6 samples of sunflower honey) from the territory of the Republic of Serbia were analyzed. Physicochemical analysis of the samples included the examination of basic quality parameters and qualitative and quantitative analyses of phenolic compounds. In the samples tested, a total of 93 phenolic compounds were tentatively identified, and 19 of them were quantified. The obtained physicochemical analysis of the data served as input for the multivariate analysis. The h..t map, which is useful for visualizing numerical data, was used for this purpose. The obtained results showed that the applied data can serve to clearly separate acacia and sunflower honeys.",
publisher = "Soc Chemists Technologists Madeconia, Skopje",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "Physicochemical parameters as indicators of the authenticity of monofloral honey from the territory of the republic of Serbia",
pages = "67-57",
number = "1",
volume = "40",
doi = "10.20450/mjcce.2021.2186"
}

Milosavljević, S., Jadranin, M., Mladenović, M., Tešević, V., Menković, N., Mutavdžić, D.,& Krstic, G.. (2021). Physicochemical parameters as indicators of the authenticity of monofloral honey from the territory of the republic of Serbia. in Macedonian Journal of Chemistry and Chemical Engineering
Soc Chemists Technologists Madeconia, Skopje., 40(1), 57-67.
https://doi.org/10.20450/mjcce.2021.2186

Milosavljević S, Jadranin M, Mladenović M, Tešević V, Menković N, Mutavdžić D, Krstic G. Physicochemical parameters as indicators of the authenticity of monofloral honey from the territory of the republic of Serbia. in Macedonian Journal of Chemistry and Chemical Engineering. 2021;40(1):57-67.
doi:10.20450/mjcce.2021.2186 .

Milosavljević, Sasa, Jadranin, Milka, Mladenović, Mica, Tešević, Vele, Menković, Nebojša, Mutavdžić, Dragosav, Krstic, Gordana, "Physicochemical parameters as indicators of the authenticity of monofloral honey from the territory of the republic of Serbia" in Macedonian Journal of Chemistry and Chemical Engineering, 40, no. 1 (2021):57-67,
https://doi.org/10.20450/mjcce.2021.2186 . .