TY  - JOUR
AU  - Živanović, Branka  D.
AU  - Ullrich, Kristian
AU  - Spasić, Slađana
AU  - Galland, Paul
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2305
AB  - Guttation, the formation of exudation water, is widespread among plants and fungi, yet the 
underlying mechanisms remain largely unknown. We describe the conditions for inducing guttation in sporangiophores of the mucoracean fungus, Phycomyces blakesleeanus. Cultivation on 
peptone-enriched potato dextrose agar elicits vigorous guttation mainly below the apical growing 
zone, while sporangiophores raised on a glucose-mineral medium manifest only moderate guttation. 
Mycelia do not guttate irrespective of the employed media. The topology of guttation droplets 
allows identifying the non-growing part of the sporangiophore as a guttation zone, which responds 
to humidity and medium composition in ways that become relevant for turgor homeostasis and thus the sensor physiology of the growing zone. Apparently, the entire sporangiophore, rather than 
exclusively the growing zone, participates in signal reception and integration to generate a common growth output. Exogenous auxin applied to the growing zones elicits two correlated responses: (i) formation of guttation droplets in the growing and transition zones below the sporangium and (ii) a diminution of the growth rate. In sporangiophore populations, guttation-induction by exogenous control buffer occurs at low frequencies; the bias for guttation increases with increasing auxin concentration. Synthetic auxins and the transport inhibitor NPA suppress guttation completely, but leave growth rates largely unaffected. Mutants C2 carA and C148 carA madC display higher sensitivities for auxin-induced guttation compared to wild type. A working model for guttation includes aquaporins and mechanosensitive ion channels that we identified in Phycomyces by sequence domain searches.
PB  - Springer-Verlag GmbH Austria, part of Springer Nature 2023
T2  - Protoplasma
T1  - Auxin‑ and pH‑induced guttation in Phycomyces sporangiophores: relation between guttation and diminished elongation growth
EP  - 1133
SP  - 1109
VL  - 260
DO  - 10.1007/s00709-022-01833-3
ER  - 

@article{
author = "Živanović, Branka  D. and Ullrich, Kristian and Spasić, Slađana and Galland, Paul",
year = "2023",
abstract = "Guttation, the formation of exudation water, is widespread among plants and fungi, yet the 
underlying mechanisms remain largely unknown. We describe the conditions for inducing guttation in sporangiophores of the mucoracean fungus, Phycomyces blakesleeanus. Cultivation on 
peptone-enriched potato dextrose agar elicits vigorous guttation mainly below the apical growing 
zone, while sporangiophores raised on a glucose-mineral medium manifest only moderate guttation. 
Mycelia do not guttate irrespective of the employed media. The topology of guttation droplets 
allows identifying the non-growing part of the sporangiophore as a guttation zone, which responds 
to humidity and medium composition in ways that become relevant for turgor homeostasis and thus the sensor physiology of the growing zone. Apparently, the entire sporangiophore, rather than 
exclusively the growing zone, participates in signal reception and integration to generate a common growth output. Exogenous auxin applied to the growing zones elicits two correlated responses: (i) formation of guttation droplets in the growing and transition zones below the sporangium and (ii) a diminution of the growth rate. In sporangiophore populations, guttation-induction by exogenous control buffer occurs at low frequencies; the bias for guttation increases with increasing auxin concentration. Synthetic auxins and the transport inhibitor NPA suppress guttation completely, but leave growth rates largely unaffected. Mutants C2 carA and C148 carA madC display higher sensitivities for auxin-induced guttation compared to wild type. A working model for guttation includes aquaporins and mechanosensitive ion channels that we identified in Phycomyces by sequence domain searches.",
publisher = "Springer-Verlag GmbH Austria, part of Springer Nature 2023",
journal = "Protoplasma",
title = "Auxin‑ and pH‑induced guttation in Phycomyces sporangiophores: relation between guttation and diminished elongation growth",
pages = "1133-1109",
volume = "260",
doi = "10.1007/s00709-022-01833-3"
}

Živanović, Branka  D., Ullrich, K., Spasić, S.,& Galland, P.. (2023). Auxin‑ and pH‑induced guttation in Phycomyces sporangiophores: relation between guttation and diminished elongation growth. in Protoplasma
Springer-Verlag GmbH Austria, part of Springer Nature 2023., 260, 1109-1133.
https://doi.org/10.1007/s00709-022-01833-3

Živanović, Branka  D., Ullrich K, Spasić S, Galland P. Auxin‑ and pH‑induced guttation in Phycomyces sporangiophores: relation between guttation and diminished elongation growth. in Protoplasma. 2023;260:1109-1133.
doi:10.1007/s00709-022-01833-3 .

Živanović, Branka  D., Ullrich, Kristian, Spasić, Slađana, Galland, Paul, "Auxin‑ and pH‑induced guttation in Phycomyces sporangiophores: relation between guttation and diminished elongation growth" in Protoplasma, 260 (2023):1109-1133,
https://doi.org/10.1007/s00709-022-01833-3 . .