TY  - JOUR
AU  - Kukavica, Biljana
AU  - Veljović-Jovanović, Sonja
AU  - Menckhoff, Ljiljana
AU  - Luethje, Sabine
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/579
AB  - Cell wall isolated from pea roots was used to separate and characterize two fractions possessing class III peroxidase activity: (i) ionically bound proteins and (ii) covalently bound proteins. Modified SDS-PAGE separated peroxidase isoforms by their apparent molecular weights: four bands of 56, 46, 44, and 41 kDa were found in the ionically bound fraction (iPOD) and one band (70 kDa) was resolved after treatment of the cell wall with cellulase and pectinase (cPOD). Isoelectric focusing (IEF) patterns for iPODs and cPODs were significantly different: five iPODs with highly cationic pI (9.5-9.2) were detected, whereas the nine cPODs were anionic with pI values between pH 3.7 and 5. iPODs and cPODs showed rather specific substrate affinity and different sensitivity to inhibitors, heat, and deglycosylation treatments. Peroxidase and oxidase activities and their IEF patterns for both fractions were determined in different zones along the root and in roots of different ages. New iPODs with pI 9.34 and 9.5 were induced with root growth, while the activity of cPODs was more related to the formation of the cell wall in non-elongating tissue. Treatment with auxin that inhibits root growth led to suppression of iPOD and induction of cPOD. A similar effect was obtained with the widely used elicitor, chitosan, which also induced cPODs with pI 5.3 and 5.7, which may be specifically related to pathogen defence. The differences reported here between biochemical properties of cPOD and iPOD and their differential induction during development and under specific treatments implicate that they are involved in specific and different physiological processes.
PB  - Oxford Univ Press, Oxford
T2  - Journal of Experimental Botany
T1  - Cell wall-bound cationic and anionic class III isoperoxidases of pea root: biochemical characterization and function in root growth
EP  - 4645
IS  - 12
SP  - 4631
VL  - 63
DO  - 10.1093/jxb/ers139
ER  - 

@article{
author = "Kukavica, Biljana and Veljović-Jovanović, Sonja and Menckhoff, Ljiljana and Luethje, Sabine",
year = "2012",
abstract = "Cell wall isolated from pea roots was used to separate and characterize two fractions possessing class III peroxidase activity: (i) ionically bound proteins and (ii) covalently bound proteins. Modified SDS-PAGE separated peroxidase isoforms by their apparent molecular weights: four bands of 56, 46, 44, and 41 kDa were found in the ionically bound fraction (iPOD) and one band (70 kDa) was resolved after treatment of the cell wall with cellulase and pectinase (cPOD). Isoelectric focusing (IEF) patterns for iPODs and cPODs were significantly different: five iPODs with highly cationic pI (9.5-9.2) were detected, whereas the nine cPODs were anionic with pI values between pH 3.7 and 5. iPODs and cPODs showed rather specific substrate affinity and different sensitivity to inhibitors, heat, and deglycosylation treatments. Peroxidase and oxidase activities and their IEF patterns for both fractions were determined in different zones along the root and in roots of different ages. New iPODs with pI 9.34 and 9.5 were induced with root growth, while the activity of cPODs was more related to the formation of the cell wall in non-elongating tissue. Treatment with auxin that inhibits root growth led to suppression of iPOD and induction of cPOD. A similar effect was obtained with the widely used elicitor, chitosan, which also induced cPODs with pI 5.3 and 5.7, which may be specifically related to pathogen defence. The differences reported here between biochemical properties of cPOD and iPOD and their differential induction during development and under specific treatments implicate that they are involved in specific and different physiological processes.",
publisher = "Oxford Univ Press, Oxford",
journal = "Journal of Experimental Botany",
title = "Cell wall-bound cationic and anionic class III isoperoxidases of pea root: biochemical characterization and function in root growth",
pages = "4645-4631",
number = "12",
volume = "63",
doi = "10.1093/jxb/ers139"
}

Kukavica, B., Veljović-Jovanović, S., Menckhoff, L.,& Luethje, S.. (2012). Cell wall-bound cationic and anionic class III isoperoxidases of pea root: biochemical characterization and function in root growth. in Journal of Experimental Botany
Oxford Univ Press, Oxford., 63(12), 4631-4645.
https://doi.org/10.1093/jxb/ers139

Kukavica B, Veljović-Jovanović S, Menckhoff L, Luethje S. Cell wall-bound cationic and anionic class III isoperoxidases of pea root: biochemical characterization and function in root growth. in Journal of Experimental Botany. 2012;63(12):4631-4645.
doi:10.1093/jxb/ers139 .

Kukavica, Biljana, Veljović-Jovanović, Sonja, Menckhoff, Ljiljana, Luethje, Sabine, "Cell wall-bound cationic and anionic class III isoperoxidases of pea root: biochemical characterization and function in root growth" in Journal of Experimental Botany, 63, no. 12 (2012):4631-4645,
https://doi.org/10.1093/jxb/ers139 . .