TY  - JOUR
AU  - Radotić, Ksenija
AU  - Roduit, Charles
AU  - Simonović Radosavljević, Jasna
AU  - Hornitschek, Patricia
AU  - Fankhauser, Christian
AU  - Mutavdžić, Dragosav
AU  - Steinbach, Gabor
AU  - Dietler, Giovanni
AU  - Kasas, Sandor
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/594
AB  - Cell-wall mechanical properties play a key role in the growth and the protection of plants. However, little is known about genuine wall mechanical properties and their growth-related dynamics at subcellular resolution and in living cells. Here, we used atomic force microscopy (AFM) stiffness tomography to explore stiffness distribution in the cell wall of suspension-cultured Arabidopsis thaliana as a model of primary, growing cell wall. For the first time that we know of, this new imaging technique was performed on living single cells of a higher plant, permitting monitoring of the stiffness distribution in cell-wall layers as a function of the depth and its evolution during the different growth phases. The mechanical measurements were correlated with changes in the composition of the cell wall, which were revealed by Fourier-transform infrared (FTIR) spectroscopy. In the beginning and end of cell growth, the average stiffness of the cell wall was low and the wall was mechanically homogenous, whereas in the exponential growth phase, the average wall stiffness increased, with increasing heterogeneity. In this phase, the difference between the superficial and deep wall stiffness was highest. FTIR spectra revealed a relative increase in the polysaccharide/lignin content.
PB  - Cell Press, Cambridge
T2  - Biophysical Journal
T1  - Atomic Force Microscopy Stiffness Tomography on Living Arabidopsis thaliana Cells Reveals the Mechanical Properties of Surface and Deep Cell-Wall Layers during Growth
EP  - 394
IS  - 3
SP  - 386
VL  - 103
DO  - 10.1016/j.bpj.2012.06.046
ER  - 

@article{
author = "Radotić, Ksenija and Roduit, Charles and Simonović Radosavljević, Jasna and Hornitschek, Patricia and Fankhauser, Christian and Mutavdžić, Dragosav and Steinbach, Gabor and Dietler, Giovanni and Kasas, Sandor",
year = "2012",
abstract = "Cell-wall mechanical properties play a key role in the growth and the protection of plants. However, little is known about genuine wall mechanical properties and their growth-related dynamics at subcellular resolution and in living cells. Here, we used atomic force microscopy (AFM) stiffness tomography to explore stiffness distribution in the cell wall of suspension-cultured Arabidopsis thaliana as a model of primary, growing cell wall. For the first time that we know of, this new imaging technique was performed on living single cells of a higher plant, permitting monitoring of the stiffness distribution in cell-wall layers as a function of the depth and its evolution during the different growth phases. The mechanical measurements were correlated with changes in the composition of the cell wall, which were revealed by Fourier-transform infrared (FTIR) spectroscopy. In the beginning and end of cell growth, the average stiffness of the cell wall was low and the wall was mechanically homogenous, whereas in the exponential growth phase, the average wall stiffness increased, with increasing heterogeneity. In this phase, the difference between the superficial and deep wall stiffness was highest. FTIR spectra revealed a relative increase in the polysaccharide/lignin content.",
publisher = "Cell Press, Cambridge",
journal = "Biophysical Journal",
title = "Atomic Force Microscopy Stiffness Tomography on Living Arabidopsis thaliana Cells Reveals the Mechanical Properties of Surface and Deep Cell-Wall Layers during Growth",
pages = "394-386",
number = "3",
volume = "103",
doi = "10.1016/j.bpj.2012.06.046"
}

Radotić, K., Roduit, C., Simonović Radosavljević, J., Hornitschek, P., Fankhauser, C., Mutavdžić, D., Steinbach, G., Dietler, G.,& Kasas, S.. (2012). Atomic Force Microscopy Stiffness Tomography on Living Arabidopsis thaliana Cells Reveals the Mechanical Properties of Surface and Deep Cell-Wall Layers during Growth. in Biophysical Journal
Cell Press, Cambridge., 103(3), 386-394.
https://doi.org/10.1016/j.bpj.2012.06.046

Radotić K, Roduit C, Simonović Radosavljević J, Hornitschek P, Fankhauser C, Mutavdžić D, Steinbach G, Dietler G, Kasas S. Atomic Force Microscopy Stiffness Tomography on Living Arabidopsis thaliana Cells Reveals the Mechanical Properties of Surface and Deep Cell-Wall Layers during Growth. in Biophysical Journal. 2012;103(3):386-394.
doi:10.1016/j.bpj.2012.06.046 .

Radotić, Ksenija, Roduit, Charles, Simonović Radosavljević, Jasna, Hornitschek, Patricia, Fankhauser, Christian, Mutavdžić, Dragosav, Steinbach, Gabor, Dietler, Giovanni, Kasas, Sandor, "Atomic Force Microscopy Stiffness Tomography on Living Arabidopsis thaliana Cells Reveals the Mechanical Properties of Surface and Deep Cell-Wall Layers during Growth" in Biophysical Journal, 103, no. 3 (2012):386-394,
https://doi.org/10.1016/j.bpj.2012.06.046 . .