TY  - CONF
AU  - Popović, Tatjana
AU  - Marković, Sanja
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1930
AB  - Transmission of plant pathogens and pests across country borders has raised an alert in the 
EU. Some of them having a severe economic impact, leading for taking precautionary 
measures such as quarantine of plant materials transported across borders. Clavibacter 
michiganensis subsp. sepedonicus (Cms) is listed as a quarantine organism in EU. This 
bacterium causes potato ring rot disease and presents a worldwide threat due to crop losses 
during vegetation and in storage. Potato tubers from import in Serbia were tested for its 
presence in accordance with the official EU Council Directive. From the stolon end of 200 
tubers (consist one sample), the small core of tissue containing vascular tissue was removed 
and the heel ends were taken and crashed in sterile phosphate buffer, then centrifuged. Re suspended pellet was used for immunofluorescence (IF, Loewe Biochemica GmbH), isolation 
of bacteria and DNA extraction for Polymerase Chain Reaction (PCR) performed with a 
pathogen-specific primer set PSA-1/PSA-R. As a positive control Cms reference strain CFBP 
3561 was used. Two samples of ware potato originated from Belarus and Russian Federation 
in 2019 gave positive results for the presence of Cms. Visible internal symptom was observed 
on several tubers, in form of the vascular ring of tuber when they cut transversely. Bacterial 
ooze stream when tubers squeezed. The isolation of bacteria was performed from the ooze on 
Nutrient dextrose agar. Creamy-white, smooth colonies were formed after 3-5 days of 
incubation at 22 ºC. Restriction fragment length polymorphism (RFLP) of PCR products 
performed with PSA-1/PSA-R primer pair (502 bp) with enzyme BglII (fragments 282 and 
220 bp in size) confirmed that the isolates belong to Cms. Pathogenicity was confirmed on 
aubergine seedlings, showing typical wilting obtained within 15-20 days after inoculation.
Serbia is still free area from Cms pathogen due to rejection of contaminated potatoes from 
import.
PB  - INTERNATIONAL Scientific Agriculture Symposium "AGROSYM"
C3  - Proceedings of the XI International Scientific Agricultural Symposium “Agrosym 2020”
T1  - RISK OF INTRODUCTION OF QUARANTINE ORGANISMS: CASE  CLAVIBACTER MICHIGANENSIS SUBSP. SEPEDONICUS
EP  - 546
SP  - 541
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1930
ER  - 

@conference{
author = "Popović, Tatjana and Marković, Sanja",
year = "2020",
abstract = "Transmission of plant pathogens and pests across country borders has raised an alert in the 
EU. Some of them having a severe economic impact, leading for taking precautionary 
measures such as quarantine of plant materials transported across borders. Clavibacter 
michiganensis subsp. sepedonicus (Cms) is listed as a quarantine organism in EU. This 
bacterium causes potato ring rot disease and presents a worldwide threat due to crop losses 
during vegetation and in storage. Potato tubers from import in Serbia were tested for its 
presence in accordance with the official EU Council Directive. From the stolon end of 200 
tubers (consist one sample), the small core of tissue containing vascular tissue was removed 
and the heel ends were taken and crashed in sterile phosphate buffer, then centrifuged. Re suspended pellet was used for immunofluorescence (IF, Loewe Biochemica GmbH), isolation 
of bacteria and DNA extraction for Polymerase Chain Reaction (PCR) performed with a 
pathogen-specific primer set PSA-1/PSA-R. As a positive control Cms reference strain CFBP 
3561 was used. Two samples of ware potato originated from Belarus and Russian Federation 
in 2019 gave positive results for the presence of Cms. Visible internal symptom was observed 
on several tubers, in form of the vascular ring of tuber when they cut transversely. Bacterial 
ooze stream when tubers squeezed. The isolation of bacteria was performed from the ooze on 
Nutrient dextrose agar. Creamy-white, smooth colonies were formed after 3-5 days of 
incubation at 22 ºC. Restriction fragment length polymorphism (RFLP) of PCR products 
performed with PSA-1/PSA-R primer pair (502 bp) with enzyme BglII (fragments 282 and 
220 bp in size) confirmed that the isolates belong to Cms. Pathogenicity was confirmed on 
aubergine seedlings, showing typical wilting obtained within 15-20 days after inoculation.
Serbia is still free area from Cms pathogen due to rejection of contaminated potatoes from 
import.",
publisher = "INTERNATIONAL Scientific Agriculture Symposium "AGROSYM"",
journal = "Proceedings of the XI International Scientific Agricultural Symposium “Agrosym 2020”",
title = "RISK OF INTRODUCTION OF QUARANTINE ORGANISMS: CASE  CLAVIBACTER MICHIGANENSIS SUBSP. SEPEDONICUS",
pages = "546-541",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1930"
}

Popović, T.,& Marković, S.. (2020). RISK OF INTRODUCTION OF QUARANTINE ORGANISMS: CASE  CLAVIBACTER MICHIGANENSIS SUBSP. SEPEDONICUS. in Proceedings of the XI International Scientific Agricultural Symposium “Agrosym 2020”
INTERNATIONAL Scientific Agriculture Symposium "AGROSYM"., 541-546.
https://hdl.handle.net/21.15107/rcub_rimsi_1930

Popović T, Marković S. RISK OF INTRODUCTION OF QUARANTINE ORGANISMS: CASE  CLAVIBACTER MICHIGANENSIS SUBSP. SEPEDONICUS. in Proceedings of the XI International Scientific Agricultural Symposium “Agrosym 2020”. 2020;:541-546.
https://hdl.handle.net/21.15107/rcub_rimsi_1930 .

Popović, Tatjana, Marković, Sanja, "RISK OF INTRODUCTION OF QUARANTINE ORGANISMS: CASE  CLAVIBACTER MICHIGANENSIS SUBSP. SEPEDONICUS" in Proceedings of the XI International Scientific Agricultural Symposium “Agrosym 2020” (2020):541-546,
https://hdl.handle.net/21.15107/rcub_rimsi_1930 .