TY  - JOUR
AU  - Živanović, Bojana
AU  - Milić Komić, Sonja
AU  - Nikolić, Nenad
AU  - Mutavdžić, Dragosav
AU  - Srećković, Tatjana
AU  - Veljović-Jovanović, Sonja
AU  - Prokic, Ljiljana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1509
AB  - Two tomato genotypes with constitutively different ABA level, flacca mutant and wild type of Ailsa Craig cv. (WT), were subjected to three repeated drought cycles, with the aim to reveal the role of the abscisic acid (ABA) threshold in developing drought tolerance. Differential responses to drought of two genotypes were obtained: more pronounced stomatal closure, ABA biosynthesis and proline accumulation in WT compared to the mutant were compensated by dry weight accumulation accompanied by transient redox disbalance in flacca. Fourier-transform infrared (FTIR) spectra analysis of isolated cell wall material and morphological parameter measurements on tomato leaves indicated changes in dry weight accumulation and carbon re-allocation to cell wall constituents in flacca, but not in WT. A higher proportion of cellulose, pectin and lignin in isolated cell walls from flacca leaves further increased with repeated drought cycles. Different ABA-dependent stomatal closure between drought cycles implies that acquisition of stomatal sensitivity may be a part of stress memory mechanism developed under given conditions. The regulatory role of ABA in the cell wall restructuring and growth regulation under low leaf potential was discussed with emphasis on the beneficial effects of drought priming in developing differential defense strategies against drought.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Differential Response of Two Tomato Genotypes, Wild Type cv. Ailsa Craig and Its ABA-Deficient Mutant flacca to Short-Termed Drought Cycles
IS  - 11
VL  - 10
DO  - 10.3390/plants10112308
ER  - 

@article{
author = "Živanović, Bojana and Milić Komić, Sonja and Nikolić, Nenad and Mutavdžić, Dragosav and Srećković, Tatjana and Veljović-Jovanović, Sonja and Prokic, Ljiljana",
year = "2021",
abstract = "Two tomato genotypes with constitutively different ABA level, flacca mutant and wild type of Ailsa Craig cv. (WT), were subjected to three repeated drought cycles, with the aim to reveal the role of the abscisic acid (ABA) threshold in developing drought tolerance. Differential responses to drought of two genotypes were obtained: more pronounced stomatal closure, ABA biosynthesis and proline accumulation in WT compared to the mutant were compensated by dry weight accumulation accompanied by transient redox disbalance in flacca. Fourier-transform infrared (FTIR) spectra analysis of isolated cell wall material and morphological parameter measurements on tomato leaves indicated changes in dry weight accumulation and carbon re-allocation to cell wall constituents in flacca, but not in WT. A higher proportion of cellulose, pectin and lignin in isolated cell walls from flacca leaves further increased with repeated drought cycles. Different ABA-dependent stomatal closure between drought cycles implies that acquisition of stomatal sensitivity may be a part of stress memory mechanism developed under given conditions. The regulatory role of ABA in the cell wall restructuring and growth regulation under low leaf potential was discussed with emphasis on the beneficial effects of drought priming in developing differential defense strategies against drought.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Differential Response of Two Tomato Genotypes, Wild Type cv. Ailsa Craig and Its ABA-Deficient Mutant flacca to Short-Termed Drought Cycles",
number = "11",
volume = "10",
doi = "10.3390/plants10112308"
}

Živanović, B., Milić Komić, S., Nikolić, N., Mutavdžić, D., Srećković, T., Veljović-Jovanović, S.,& Prokic, L.. (2021). Differential Response of Two Tomato Genotypes, Wild Type cv. Ailsa Craig and Its ABA-Deficient Mutant flacca to Short-Termed Drought Cycles. in Plants-Basel
MDPI, Basel., 10(11).
https://doi.org/10.3390/plants10112308

Živanović B, Milić Komić S, Nikolić N, Mutavdžić D, Srećković T, Veljović-Jovanović S, Prokic L. Differential Response of Two Tomato Genotypes, Wild Type cv. Ailsa Craig and Its ABA-Deficient Mutant flacca to Short-Termed Drought Cycles. in Plants-Basel. 2021;10(11).
doi:10.3390/plants10112308 .

Živanović, Bojana, Milić Komić, Sonja, Nikolić, Nenad, Mutavdžić, Dragosav, Srećković, Tatjana, Veljović-Jovanović, Sonja, Prokic, Ljiljana, "Differential Response of Two Tomato Genotypes, Wild Type cv. Ailsa Craig and Its ABA-Deficient Mutant flacca to Short-Termed Drought Cycles" in Plants-Basel, 10, no. 11 (2021),
https://doi.org/10.3390/plants10112308 . .

TY  - JOUR
AU  - Vidović, Marija
AU  - Cuković, Katarina
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1339
AB  - Resurrection plant Ramonda serbica is a suitable model to investigate mechanisms of desiccation tolerance, while variegated Pelargonium zonale has been proven to serve as an excellent model for the metabolite allocation between sink tissue and source tissue within the same organ. However, the genomes of these plants are still not sequenced, limiting their application in molecular studies. To investigate the transcript abundance by next-generation sequencing, high-quality RNA input is required. Leaves of both P. zonale and R. serbica are rich in polyphenols that interfere with high-quality RNA extraction by common protocols. Moreover, low water content and high amount of sugars and other osmoprotectants in desiccated R. serbica leaves present the additional challenge in total RNA extraction. Here, we evaluated and compared several already established TRIzol- and CTAB-based protocols aiming to develop the efficient, simple and low-cost methods for the extraction of the satisfactory yield RNA of great purity and integrity, required for the construction of high-quality cDNA libraries. Our results show that the CTAB-based protocol (i.e. CTAB 1b) enabled the extraction of high-quality RNA from photosynthetically active and non-photosynthetically active leaf sectors of P. zonale, with high RIN values. On the other hand, TRIzol-based protocol provided a high RNA yield with low contamination and high RNA integrity even in desiccated leaves of R. serbica. We envisage that the proposed protocol would be suitable for the RNA extractions from other desiccated organs (e.g. seeds, grains, pollen grains).
PB  - Springer Heidelberg, Heidelberg
T2  - 3 Biotech
T1  - Isolation of high-quality RNA from recalcitrant leaves of variegated and resurrection plants
IS  - 6
VL  - 10
DO  - 10.1007/s13205-020-02279-1
ER  - 

@article{
author = "Vidović, Marija and Cuković, Katarina",
year = "2020",
abstract = "Resurrection plant Ramonda serbica is a suitable model to investigate mechanisms of desiccation tolerance, while variegated Pelargonium zonale has been proven to serve as an excellent model for the metabolite allocation between sink tissue and source tissue within the same organ. However, the genomes of these plants are still not sequenced, limiting their application in molecular studies. To investigate the transcript abundance by next-generation sequencing, high-quality RNA input is required. Leaves of both P. zonale and R. serbica are rich in polyphenols that interfere with high-quality RNA extraction by common protocols. Moreover, low water content and high amount of sugars and other osmoprotectants in desiccated R. serbica leaves present the additional challenge in total RNA extraction. Here, we evaluated and compared several already established TRIzol- and CTAB-based protocols aiming to develop the efficient, simple and low-cost methods for the extraction of the satisfactory yield RNA of great purity and integrity, required for the construction of high-quality cDNA libraries. Our results show that the CTAB-based protocol (i.e. CTAB 1b) enabled the extraction of high-quality RNA from photosynthetically active and non-photosynthetically active leaf sectors of P. zonale, with high RIN values. On the other hand, TRIzol-based protocol provided a high RNA yield with low contamination and high RNA integrity even in desiccated leaves of R. serbica. We envisage that the proposed protocol would be suitable for the RNA extractions from other desiccated organs (e.g. seeds, grains, pollen grains).",
publisher = "Springer Heidelberg, Heidelberg",
journal = "3 Biotech",
title = "Isolation of high-quality RNA from recalcitrant leaves of variegated and resurrection plants",
number = "6",
volume = "10",
doi = "10.1007/s13205-020-02279-1"
}

Vidović, M.,& Cuković, K.. (2020). Isolation of high-quality RNA from recalcitrant leaves of variegated and resurrection plants. in 3 Biotech
Springer Heidelberg, Heidelberg., 10(6).
https://doi.org/10.1007/s13205-020-02279-1

Vidović M, Cuković K. Isolation of high-quality RNA from recalcitrant leaves of variegated and resurrection plants. in 3 Biotech. 2020;10(6).
doi:10.1007/s13205-020-02279-1 .

Vidović, Marija, Cuković, Katarina, "Isolation of high-quality RNA from recalcitrant leaves of variegated and resurrection plants" in 3 Biotech, 10, no. 6 (2020),
https://doi.org/10.1007/s13205-020-02279-1 . .