TY  - JOUR
AU  - Ajdzanović, Vladimir Z
AU  - Spasojević, Ivan
AU  - Sosic-Jurjević, Branka T
AU  - Filipović, Branko R
AU  - Trifunović, Svetlana L
AU  - Sekulic, Milka I
AU  - Milošević, Verica Lj.
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/482
AB  - A decrease of erythrocyte membrane fluidity can contribute to the pathophysiology of hypertension. Soy products, which are used as alternative therapeutics in some cardiovascular conditions, contain various isoflavones (genistein, daidzein, and their glucosides, genistin and daidzin), which can incorporate cellular membrane and change its fluidity. The aim of this study was to examine the effects of soy extract (which generally corresponds to the soy products of isoflavone composition) on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance spectroscopy and fatty acid spin probes (5-DS and 12-DS), the spectra of which are dependent on membrane fluidity. After being treated with soy extract, erythrocytes showed a significant (P = 0.016) decrease of membrane fluidity near the hydrophilic surface, while there were no significant changes of fluidity in deeper hydrophobic membrane regions. These results suggest that soy products containing high levels of genistein and isoflavone glucosides may not be suitable for use in hypertension because they decrease erythrocyte membrane fluidity.
PB  - Springer, New York
T2  - Journal of Membrane Biology
T1  - The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study
EP  - 135
IS  - 3
SP  - 131
VL  - 239
DO  - 10.1007/s00232-010-9332-8
ER  - 

@article{
author = "Ajdzanović, Vladimir Z and Spasojević, Ivan and Sosic-Jurjević, Branka T and Filipović, Branko R and Trifunović, Svetlana L and Sekulic, Milka I and Milošević, Verica Lj.",
year = "2011",
abstract = "A decrease of erythrocyte membrane fluidity can contribute to the pathophysiology of hypertension. Soy products, which are used as alternative therapeutics in some cardiovascular conditions, contain various isoflavones (genistein, daidzein, and their glucosides, genistin and daidzin), which can incorporate cellular membrane and change its fluidity. The aim of this study was to examine the effects of soy extract (which generally corresponds to the soy products of isoflavone composition) on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance spectroscopy and fatty acid spin probes (5-DS and 12-DS), the spectra of which are dependent on membrane fluidity. After being treated with soy extract, erythrocytes showed a significant (P = 0.016) decrease of membrane fluidity near the hydrophilic surface, while there were no significant changes of fluidity in deeper hydrophobic membrane regions. These results suggest that soy products containing high levels of genistein and isoflavone glucosides may not be suitable for use in hypertension because they decrease erythrocyte membrane fluidity.",
publisher = "Springer, New York",
journal = "Journal of Membrane Biology",
title = "The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study",
pages = "135-131",
number = "3",
volume = "239",
doi = "10.1007/s00232-010-9332-8"
}

Ajdzanović, V. Z., Spasojević, I., Sosic-Jurjević, B. T., Filipović, B. R., Trifunović, S. L., Sekulic, M. I.,& Milošević, V. Lj.. (2011). The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study. in Journal of Membrane Biology
Springer, New York., 239(3), 131-135.
https://doi.org/10.1007/s00232-010-9332-8

Ajdzanović VZ, Spasojević I, Sosic-Jurjević BT, Filipović BR, Trifunović SL, Sekulic MI, Milošević VL. The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study. in Journal of Membrane Biology. 2011;239(3):131-135.
doi:10.1007/s00232-010-9332-8 .

Ajdzanović, Vladimir Z, Spasojević, Ivan, Sosic-Jurjević, Branka T, Filipović, Branko R, Trifunović, Svetlana L, Sekulic, Milka I, Milošević, Verica Lj., "The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study" in Journal of Membrane Biology, 239, no. 3 (2011):131-135,
https://doi.org/10.1007/s00232-010-9332-8 . .

TY  - JOUR
AU  - Ajdzanović, Vladimir Z
AU  - Spasojević, Ivan
AU  - Filipović, Branko R
AU  - Sosic-Jurjević, Branka T
AU  - Sekulic, Milka I
AU  - Milošević, Verica Lj.
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/414
AB  - The maintenance of erythrocyte membrane fluidity at the physiological level is an important factor affecting the ability of erythrocytes to pass through blood vessels of small luminal diameter. Genistein and daidzein, which are used as alternative therapeutics in cardiovascular conditions, can be incorporated into the cell membrane and change its fluidity. The aim of this study was to examine the effects of genistein and daidzein on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance (EPR) spectroscopy and fatty acid spin probes (5-DS and 12-DS) where EPR spectra were dependent on fluidity. The results showed that genistein significantly (p  lt  0.05) decreased erythrocyte membrane fluidity near the hydrophilic surface, while daidzein significantly (p  lt  0.05) increased the same parameter in deeper regions of the membrane. These data suggest that the deep fluidizing effects of daidzein on erythrocyte membranes make it a better therapeutic choice than genistein in some cardiovascular conditions.
PB  - Natl Research Council Canada-N R C Research Press, Ottawa
T2  - Canadian Journal of Physiology and Pharmacology
T1  - Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study
EP  - 500
IS  - 4
SP  - 497
VL  - 88
DO  - 10.1139/Y10-020
ER  - 

@article{
author = "Ajdzanović, Vladimir Z and Spasojević, Ivan and Filipović, Branko R and Sosic-Jurjević, Branka T and Sekulic, Milka I and Milošević, Verica Lj.",
year = "2010",
abstract = "The maintenance of erythrocyte membrane fluidity at the physiological level is an important factor affecting the ability of erythrocytes to pass through blood vessels of small luminal diameter. Genistein and daidzein, which are used as alternative therapeutics in cardiovascular conditions, can be incorporated into the cell membrane and change its fluidity. The aim of this study was to examine the effects of genistein and daidzein on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance (EPR) spectroscopy and fatty acid spin probes (5-DS and 12-DS) where EPR spectra were dependent on fluidity. The results showed that genistein significantly (p  lt  0.05) decreased erythrocyte membrane fluidity near the hydrophilic surface, while daidzein significantly (p  lt  0.05) increased the same parameter in deeper regions of the membrane. These data suggest that the deep fluidizing effects of daidzein on erythrocyte membranes make it a better therapeutic choice than genistein in some cardiovascular conditions.",
publisher = "Natl Research Council Canada-N R C Research Press, Ottawa",
journal = "Canadian Journal of Physiology and Pharmacology",
title = "Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study",
pages = "500-497",
number = "4",
volume = "88",
doi = "10.1139/Y10-020"
}

Ajdzanović, V. Z., Spasojević, I., Filipović, B. R., Sosic-Jurjević, B. T., Sekulic, M. I.,& Milošević, V. Lj.. (2010). Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study. in Canadian Journal of Physiology and Pharmacology
Natl Research Council Canada-N R C Research Press, Ottawa., 88(4), 497-500.
https://doi.org/10.1139/Y10-020

Ajdzanović VZ, Spasojević I, Filipović BR, Sosic-Jurjević BT, Sekulic MI, Milošević VL. Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study. in Canadian Journal of Physiology and Pharmacology. 2010;88(4):497-500.
doi:10.1139/Y10-020 .

Ajdzanović, Vladimir Z, Spasojević, Ivan, Filipović, Branko R, Sosic-Jurjević, Branka T, Sekulic, Milka I, Milošević, Verica Lj., "Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study" in Canadian Journal of Physiology and Pharmacology, 88, no. 4 (2010):497-500,
https://doi.org/10.1139/Y10-020 . .