TY  - JOUR
AU  - Vojvodić, Snežana
AU  - Stanić, Marina
AU  - Zechmann, Bernd
AU  - Ducic, Tanja
AU  - Žižić, Milan
AU  - Dimitrijević, Milena
AU  - Danilović Luković, Jelena
AU  - Milenković, Milica R.
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1354
AB  - Microalgae have evolved mechanisms to respond to changes in copper ion availability, which are very important for normal cellular function, to tolerate metal pollution of aquatic ecosystems, and for modulation of copper bioavailability and toxicity to other organisms. Knowledge and application of these mechanisms will benefit the use of microalgae in wastewater processing and biomass production, and the use of copper compounds in the suppression of harmful algal blooms. Here, using electron microscopy, synchrotron radiation-based Fourier transform infrared spectroscopy, electron paramagnetic resonance spectroscopy, and X-ray absorption fine structure spectroscopy, we show that the microalga Chlorella sorokiniana responds promptly to Cu2+ at high non-toxic concentration, by mucilage release, alterations in the architecture of the outer cell wall layer and lipid structures, and polyphosphate accumulation within mucilage matrix. The main route of copper detoxification is by Cu2+ coordination to polyphosphates in penta-coordinated geometry. The sequestrated Cu2+ was accessible and could be released by extracellular chelating agents. Finally, the reduction in Cu2+ to Cu1+ appears also to take place. These findings reveal the biochemical basis of the capacity of microalgae to adapt to high external copper concentrations and to serve as both, sinks and pools of environmental copper.
PB  - Portland Press Ltd, London
T2  - Biochemical Journal
T1  - Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana
EP  - 3741
IS  - 19
SP  - 3729
VL  - 477
DO  - 10.1042/BCJ20200600
ER  - 

@article{
author = "Vojvodić, Snežana and Stanić, Marina and Zechmann, Bernd and Ducic, Tanja and Žižić, Milan and Dimitrijević, Milena and Danilović Luković, Jelena and Milenković, Milica R. and Pittman, Jon K. and Spasojević, Ivan",
year = "2020",
abstract = "Microalgae have evolved mechanisms to respond to changes in copper ion availability, which are very important for normal cellular function, to tolerate metal pollution of aquatic ecosystems, and for modulation of copper bioavailability and toxicity to other organisms. Knowledge and application of these mechanisms will benefit the use of microalgae in wastewater processing and biomass production, and the use of copper compounds in the suppression of harmful algal blooms. Here, using electron microscopy, synchrotron radiation-based Fourier transform infrared spectroscopy, electron paramagnetic resonance spectroscopy, and X-ray absorption fine structure spectroscopy, we show that the microalga Chlorella sorokiniana responds promptly to Cu2+ at high non-toxic concentration, by mucilage release, alterations in the architecture of the outer cell wall layer and lipid structures, and polyphosphate accumulation within mucilage matrix. The main route of copper detoxification is by Cu2+ coordination to polyphosphates in penta-coordinated geometry. The sequestrated Cu2+ was accessible and could be released by extracellular chelating agents. Finally, the reduction in Cu2+ to Cu1+ appears also to take place. These findings reveal the biochemical basis of the capacity of microalgae to adapt to high external copper concentrations and to serve as both, sinks and pools of environmental copper.",
publisher = "Portland Press Ltd, London",
journal = "Biochemical Journal",
title = "Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana",
pages = "3741-3729",
number = "19",
volume = "477",
doi = "10.1042/BCJ20200600"
}

Vojvodić, S., Stanić, M., Zechmann, B., Ducic, T., Žižić, M., Dimitrijević, M., Danilović Luković, J., Milenković, M. R., Pittman, J. K.,& Spasojević, I.. (2020). Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana. in Biochemical Journal
Portland Press Ltd, London., 477(19), 3729-3741.
https://doi.org/10.1042/BCJ20200600

Vojvodić S, Stanić M, Zechmann B, Ducic T, Žižić M, Dimitrijević M, Danilović Luković J, Milenković MR, Pittman JK, Spasojević I. Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana. in Biochemical Journal. 2020;477(19):3729-3741.
doi:10.1042/BCJ20200600 .

Vojvodić, Snežana, Stanić, Marina, Zechmann, Bernd, Ducic, Tanja, Žižić, Milan, Dimitrijević, Milena, Danilović Luković, Jelena, Milenković, Milica R., Pittman, Jon K., Spasojević, Ivan, "Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana" in Biochemical Journal, 477, no. 19 (2020):3729-3741,
https://doi.org/10.1042/BCJ20200600 . .

TY  - JOUR
AU  - Vojvodić, Snežana
AU  - Danilović Luković, Jelena
AU  - Zechmann, Bernd
AU  - Jevtović, Mima
AU  - Bogdanović Pristov, Jelena
AU  - Stanić, Marina
AU  - Lizzul, Alessandro Marco
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1371
AB  - The impact of ionizing radiation on microorganisms such as microalgae is a topic of increasing importance for understanding the dynamics of aquatic ecosystems in response to environmental radiation, and for the development of efficient approaches for bioremediation of mining and nuclear power plants wastewaters. Currently, nothing is known about the effects of ionizing radiation on the microalgal cell wall, which represents the first line of defence against chemical and physical environmental stresses. Using various microscopy, spectroscopy and biochemical techniques we show that the unicellular alga Chlorella sorokiniana elicits a fast response to ionizing radiation. Within one day after irradiation with doses of 1-5 Gy, the fibrilar layer of the cell wall became thicker, the fraction of uronic acids was higher, and the capacity to remove the main reactive product of water radiolysis increased. In addition, the isolated cell wall fraction showed significant binding capacity for Cu2+, Mn2+, and Cr3+. The irradiation further increased the binding capacity for Cu2+, which appears to be mainly bound to glucosamine moieties within a chitosan-like polymer in the outer rigid layer of the wall. These results imply that the cell wall represents a dynamic structure that is involved in the protective response of microalgae to ionizing radiation. It appears that microalgae may exhibit a significant control of metal mobility in aquatic ecosystems via biosorption by the cell wall matrix.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Chemosphere
T1  - The effects of ionizing radiation on the structure and antioxidative and metal-binding capacity of the cell wall of microalga Chlorella sorokiniana
VL  - 260
DO  - 10.1016/j.chemosphere.2020.127553
ER  - 

@article{
author = "Vojvodić, Snežana and Danilović Luković, Jelena and Zechmann, Bernd and Jevtović, Mima and Bogdanović Pristov, Jelena and Stanić, Marina and Lizzul, Alessandro Marco and Pittman, Jon K. and Spasojević, Ivan",
year = "2020",
abstract = "The impact of ionizing radiation on microorganisms such as microalgae is a topic of increasing importance for understanding the dynamics of aquatic ecosystems in response to environmental radiation, and for the development of efficient approaches for bioremediation of mining and nuclear power plants wastewaters. Currently, nothing is known about the effects of ionizing radiation on the microalgal cell wall, which represents the first line of defence against chemical and physical environmental stresses. Using various microscopy, spectroscopy and biochemical techniques we show that the unicellular alga Chlorella sorokiniana elicits a fast response to ionizing radiation. Within one day after irradiation with doses of 1-5 Gy, the fibrilar layer of the cell wall became thicker, the fraction of uronic acids was higher, and the capacity to remove the main reactive product of water radiolysis increased. In addition, the isolated cell wall fraction showed significant binding capacity for Cu2+, Mn2+, and Cr3+. The irradiation further increased the binding capacity for Cu2+, which appears to be mainly bound to glucosamine moieties within a chitosan-like polymer in the outer rigid layer of the wall. These results imply that the cell wall represents a dynamic structure that is involved in the protective response of microalgae to ionizing radiation. It appears that microalgae may exhibit a significant control of metal mobility in aquatic ecosystems via biosorption by the cell wall matrix.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Chemosphere",
title = "The effects of ionizing radiation on the structure and antioxidative and metal-binding capacity of the cell wall of microalga Chlorella sorokiniana",
volume = "260",
doi = "10.1016/j.chemosphere.2020.127553"
}

Vojvodić, S., Danilović Luković, J., Zechmann, B., Jevtović, M., Bogdanović Pristov, J., Stanić, M., Lizzul, A. M., Pittman, J. K.,& Spasojević, I.. (2020). The effects of ionizing radiation on the structure and antioxidative and metal-binding capacity of the cell wall of microalga Chlorella sorokiniana. in Chemosphere
Pergamon-Elsevier Science Ltd, Oxford., 260.
https://doi.org/10.1016/j.chemosphere.2020.127553

Vojvodić S, Danilović Luković J, Zechmann B, Jevtović M, Bogdanović Pristov J, Stanić M, Lizzul AM, Pittman JK, Spasojević I. The effects of ionizing radiation on the structure and antioxidative and metal-binding capacity of the cell wall of microalga Chlorella sorokiniana. in Chemosphere. 2020;260.
doi:10.1016/j.chemosphere.2020.127553 .

Vojvodić, Snežana, Danilović Luković, Jelena, Zechmann, Bernd, Jevtović, Mima, Bogdanović Pristov, Jelena, Stanić, Marina, Lizzul, Alessandro Marco, Pittman, Jon K., Spasojević, Ivan, "The effects of ionizing radiation on the structure and antioxidative and metal-binding capacity of the cell wall of microalga Chlorella sorokiniana" in Chemosphere, 260 (2020),
https://doi.org/10.1016/j.chemosphere.2020.127553 . .