TY  - JOUR
AU  - Donaldson, Lloyd
AU  - Radotić, Ksenija
AU  - Kalauzi, Aleksandar
AU  - Đikanović, Daniela
AU  - Jeremic, Milorad G
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/418
AB  - Confocal fluorescence microscopy was used to examine the spectral characteristics of lignin autofluorescence in secondary cell walls of normal and compression wood from Pinus radiata. Using UV excitation, fluorescence spectra of normal and compression wood sections showed significant differences, especially in the outer secondary cell wall of tracheids, with a shift in maxima from violet to blue wavelengths between normal and compression wood. A comparison of normal wood, mild and severe compression wood, showed that the wavelength shift was intermediate in the mild compression wood compared to the severe compression wood, thus offering the possibility of quantifying the severity by measuring ratios of fluorescence at violet and blue wavelengths. Fluorescence induced by blue light, rather than UV, was less well differentiated amongst wood types. Spectral deconvolution indicated the presence of a minimum of five discrete lignin fluorophores in the cell walls of both normal and compression wood tracheids. Comparison with lignin model compounds suggest that the wavelength shift may correspond in part to increased levels of p-hydroxy type lignin in the compression wood samples. The combination of confocal fluorescence imaging and related spectral deconvolution therefore offers a novel technique for characterising cell wall lignin in situ.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Journal of Structural Biology
T1  - Quantification of compression wood severity in tracheids of Pinus radiata D. Don using confocal fluorescence imaging and spectral deconvolution
EP  - 115
IS  - 1
SP  - 106
VL  - 169
DO  - 10.1016/j.jsb.2009.09.006
ER  - 

@article{
author = "Donaldson, Lloyd and Radotić, Ksenija and Kalauzi, Aleksandar and Đikanović, Daniela and Jeremic, Milorad G",
year = "2010",
abstract = "Confocal fluorescence microscopy was used to examine the spectral characteristics of lignin autofluorescence in secondary cell walls of normal and compression wood from Pinus radiata. Using UV excitation, fluorescence spectra of normal and compression wood sections showed significant differences, especially in the outer secondary cell wall of tracheids, with a shift in maxima from violet to blue wavelengths between normal and compression wood. A comparison of normal wood, mild and severe compression wood, showed that the wavelength shift was intermediate in the mild compression wood compared to the severe compression wood, thus offering the possibility of quantifying the severity by measuring ratios of fluorescence at violet and blue wavelengths. Fluorescence induced by blue light, rather than UV, was less well differentiated amongst wood types. Spectral deconvolution indicated the presence of a minimum of five discrete lignin fluorophores in the cell walls of both normal and compression wood tracheids. Comparison with lignin model compounds suggest that the wavelength shift may correspond in part to increased levels of p-hydroxy type lignin in the compression wood samples. The combination of confocal fluorescence imaging and related spectral deconvolution therefore offers a novel technique for characterising cell wall lignin in situ.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Journal of Structural Biology",
title = "Quantification of compression wood severity in tracheids of Pinus radiata D. Don using confocal fluorescence imaging and spectral deconvolution",
pages = "115-106",
number = "1",
volume = "169",
doi = "10.1016/j.jsb.2009.09.006"
}

Donaldson, L., Radotić, K., Kalauzi, A., Đikanović, D.,& Jeremic, M. G.. (2010). Quantification of compression wood severity in tracheids of Pinus radiata D. Don using confocal fluorescence imaging and spectral deconvolution. in Journal of Structural Biology
Academic Press Inc Elsevier Science, San Diego., 169(1), 106-115.
https://doi.org/10.1016/j.jsb.2009.09.006

Donaldson L, Radotić K, Kalauzi A, Đikanović D, Jeremic MG. Quantification of compression wood severity in tracheids of Pinus radiata D. Don using confocal fluorescence imaging and spectral deconvolution. in Journal of Structural Biology. 2010;169(1):106-115.
doi:10.1016/j.jsb.2009.09.006 .

Donaldson, Lloyd, Radotić, Ksenija, Kalauzi, Aleksandar, Đikanović, Daniela, Jeremic, Milorad G, "Quantification of compression wood severity in tracheids of Pinus radiata D. Don using confocal fluorescence imaging and spectral deconvolution" in Journal of Structural Biology, 169, no. 1 (2010):106-115,
https://doi.org/10.1016/j.jsb.2009.09.006 . .