TY  - JOUR
AU  - Divović, Danka
AU  - Bogdanović Pristov, Jelena
AU  - Đikanović, Daniela
AU  - Ristic, Ivan
AU  - Radotić, Ksenija
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/916
AB  - Due to the challenges of cell walls (biomass) and its applications in various new technologies, there is a need of rapid and reliable screening of fine variations in lignin structure. The in vitro synthesized lignin model polymers are good experimental system to relate lignin structure/properties with its applications. We used iso-electric focusing electrophoresis (IEF) and fluorescence spectroscopy for screening fine structural variations in lignin model polymers, synthesized from the three lignin monomers, coniferyl alcohol, ferulic acid and p-coumaric acid, mixed in various ratios. The results were related with the thermal behavior of the polymers, revealed by differential scanning calorimetry. Each polymer had characteristic IEF pattern that can be used as its fingerprint. On the basis of the number and intensity of particular bands, it is possible to detect fine differences between polymer patterns, associated with the charge distribution on the polymer fractions. The blue shift of the main fluorescence maximum position of the polymers increased in the same order as temperature of glass transition, i. e. (polymer from coniferyl alcohol)>[polymer from coniferyl alcohol and ferulic acid 9: 1 (w/w)>[(polymer from coniferyl alcohol, ferulic acid and p-coumaric acid 8: 1: 1)>(polymer from coniferyl alcohol and p-coumaric acid 9: 1). The results show that the proposed combination of the fluorescence method and IEF may be used to gain complementary information on fine structural differences among the polymers, and influence of the types and ratios of the monomers building the polymer structure.
PB  - Springer, New York
T2  - Journal of Polymers and the Environment
T1  - Combining Electrophoretic and Fluorescence Method for Screening Fine Structural Variations Among Lignin Model Polymers Differing in Monomer Composition
EP  - 241
IS  - 2
SP  - 235
VL  - 23
DO  - 10.1007/s10924-014-0695-2
ER  - 

@article{
author = "Divović, Danka and Bogdanović Pristov, Jelena and Đikanović, Daniela and Ristic, Ivan and Radotić, Ksenija",
year = "2015",
abstract = "Due to the challenges of cell walls (biomass) and its applications in various new technologies, there is a need of rapid and reliable screening of fine variations in lignin structure. The in vitro synthesized lignin model polymers are good experimental system to relate lignin structure/properties with its applications. We used iso-electric focusing electrophoresis (IEF) and fluorescence spectroscopy for screening fine structural variations in lignin model polymers, synthesized from the three lignin monomers, coniferyl alcohol, ferulic acid and p-coumaric acid, mixed in various ratios. The results were related with the thermal behavior of the polymers, revealed by differential scanning calorimetry. Each polymer had characteristic IEF pattern that can be used as its fingerprint. On the basis of the number and intensity of particular bands, it is possible to detect fine differences between polymer patterns, associated with the charge distribution on the polymer fractions. The blue shift of the main fluorescence maximum position of the polymers increased in the same order as temperature of glass transition, i. e. (polymer from coniferyl alcohol)>[polymer from coniferyl alcohol and ferulic acid 9: 1 (w/w)>[(polymer from coniferyl alcohol, ferulic acid and p-coumaric acid 8: 1: 1)>(polymer from coniferyl alcohol and p-coumaric acid 9: 1). The results show that the proposed combination of the fluorescence method and IEF may be used to gain complementary information on fine structural differences among the polymers, and influence of the types and ratios of the monomers building the polymer structure.",
publisher = "Springer, New York",
journal = "Journal of Polymers and the Environment",
title = "Combining Electrophoretic and Fluorescence Method for Screening Fine Structural Variations Among Lignin Model Polymers Differing in Monomer Composition",
pages = "241-235",
number = "2",
volume = "23",
doi = "10.1007/s10924-014-0695-2"
}

Divović, D., Bogdanović Pristov, J., Đikanović, D., Ristic, I.,& Radotić, K.. (2015). Combining Electrophoretic and Fluorescence Method for Screening Fine Structural Variations Among Lignin Model Polymers Differing in Monomer Composition. in Journal of Polymers and the Environment
Springer, New York., 23(2), 235-241.
https://doi.org/10.1007/s10924-014-0695-2

Divović D, Bogdanović Pristov J, Đikanović D, Ristic I, Radotić K. Combining Electrophoretic and Fluorescence Method for Screening Fine Structural Variations Among Lignin Model Polymers Differing in Monomer Composition. in Journal of Polymers and the Environment. 2015;23(2):235-241.
doi:10.1007/s10924-014-0695-2 .

Divović, Danka, Bogdanović Pristov, Jelena, Đikanović, Daniela, Ristic, Ivan, Radotić, Ksenija, "Combining Electrophoretic and Fluorescence Method for Screening Fine Structural Variations Among Lignin Model Polymers Differing in Monomer Composition" in Journal of Polymers and the Environment, 23, no. 2 (2015):235-241,
https://doi.org/10.1007/s10924-014-0695-2 . .

TY  - JOUR
AU  - Đikanović, Daniela
AU  - Simonović Radosavljević, Jasna
AU  - Savić, Aleksandar G
AU  - Ristic, Ivan S
AU  - Bajuk-Bogdanović, Danica
AU  - Kalauzi, Aleksandar
AU  - Cakic, Suzana M
AU  - Budinski-Simendic, Jaroslava K
AU  - Jeremic, Milorad G
AU  - Radotić, Ksenija
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/536
AB  - In a plant cell wall, lignin is synthesized from several monomeric precursors, combined in various ratios. The variation in monomer type and quantity enables multifunctional role of lignin in plants. Thus, it is important to know how different combinations of lignin monomers impact variability of bond types and local structural changes in the polymer. Lignin model polymers are a good model system for studies of relation between variations of the starting monomers and structural variations within the polymer. We synthesized lignin model polymers from three monomers, CF-based on coniferyl alcohol and ferulic acid in monomer proportions 5:1 and 10:1 (w/w), CP-based on coniferyl alcohol and -coumaric acid in proportion 10:1 (w/w) and CA-based on pure coniferyl alcohol. We studied structural modifications in the obtained polymers, by combining fluorescence microscopy and spectroscopy, FT-IR and Raman spectroscopy, in parallel with determination of polymers' molecular mass distribution. The differences in the low (w) region of the distribution curves of the 10:1 polymers in comparison with the CA polymer may be connected with the increased content of C=C bonds and decreased content of condensed structures, as observed in FT-IR spectra and indicated by the analysis of fluorescence spectra. The 5:1 CF polymer contains a different type of structure in comparison with the 10:1 CF polymers, reflected in its simpler (w) distribution, higher homogeneity of the fluorescence emitting structures and in the appearance of a new high-wavelength emission component. We propose that this component may originate from -conjugated chains, which are longer in this polymer. The results are a contribution to the understanding of the involvement of structural variations of lignin polymers in the cell wall structural plasticity.
PB  - Springer, New York
T2  - Journal of Polymers and the Environment
T1  - Structural Differences Between Lignin Model Polymers Synthesized from Various Monomers
EP  - 617
IS  - 2
SP  - 607
VL  - 20
DO  - 10.1007/s10924-012-0422-9
ER  - 

@article{
author = "Đikanović, Daniela and Simonović Radosavljević, Jasna and Savić, Aleksandar G and Ristic, Ivan S and Bajuk-Bogdanović, Danica and Kalauzi, Aleksandar and Cakic, Suzana M and Budinski-Simendic, Jaroslava K and Jeremic, Milorad G and Radotić, Ksenija",
year = "2012",
abstract = "In a plant cell wall, lignin is synthesized from several monomeric precursors, combined in various ratios. The variation in monomer type and quantity enables multifunctional role of lignin in plants. Thus, it is important to know how different combinations of lignin monomers impact variability of bond types and local structural changes in the polymer. Lignin model polymers are a good model system for studies of relation between variations of the starting monomers and structural variations within the polymer. We synthesized lignin model polymers from three monomers, CF-based on coniferyl alcohol and ferulic acid in monomer proportions 5:1 and 10:1 (w/w), CP-based on coniferyl alcohol and -coumaric acid in proportion 10:1 (w/w) and CA-based on pure coniferyl alcohol. We studied structural modifications in the obtained polymers, by combining fluorescence microscopy and spectroscopy, FT-IR and Raman spectroscopy, in parallel with determination of polymers' molecular mass distribution. The differences in the low (w) region of the distribution curves of the 10:1 polymers in comparison with the CA polymer may be connected with the increased content of C=C bonds and decreased content of condensed structures, as observed in FT-IR spectra and indicated by the analysis of fluorescence spectra. The 5:1 CF polymer contains a different type of structure in comparison with the 10:1 CF polymers, reflected in its simpler (w) distribution, higher homogeneity of the fluorescence emitting structures and in the appearance of a new high-wavelength emission component. We propose that this component may originate from -conjugated chains, which are longer in this polymer. The results are a contribution to the understanding of the involvement of structural variations of lignin polymers in the cell wall structural plasticity.",
publisher = "Springer, New York",
journal = "Journal of Polymers and the Environment",
title = "Structural Differences Between Lignin Model Polymers Synthesized from Various Monomers",
pages = "617-607",
number = "2",
volume = "20",
doi = "10.1007/s10924-012-0422-9"
}

Đikanović, D., Simonović Radosavljević, J., Savić, A. G., Ristic, I. S., Bajuk-Bogdanović, D., Kalauzi, A., Cakic, S. M., Budinski-Simendic, J. K., Jeremic, M. G.,& Radotić, K.. (2012). Structural Differences Between Lignin Model Polymers Synthesized from Various Monomers. in Journal of Polymers and the Environment
Springer, New York., 20(2), 607-617.
https://doi.org/10.1007/s10924-012-0422-9

Đikanović D, Simonović Radosavljević J, Savić AG, Ristic IS, Bajuk-Bogdanović D, Kalauzi A, Cakic SM, Budinski-Simendic JK, Jeremic MG, Radotić K. Structural Differences Between Lignin Model Polymers Synthesized from Various Monomers. in Journal of Polymers and the Environment. 2012;20(2):607-617.
doi:10.1007/s10924-012-0422-9 .

Đikanović, Daniela, Simonović Radosavljević, Jasna, Savić, Aleksandar G, Ristic, Ivan S, Bajuk-Bogdanović, Danica, Kalauzi, Aleksandar, Cakic, Suzana M, Budinski-Simendic, Jaroslava K, Jeremic, Milorad G, Radotić, Ksenija, "Structural Differences Between Lignin Model Polymers Synthesized from Various Monomers" in Journal of Polymers and the Environment, 20, no. 2 (2012):607-617,
https://doi.org/10.1007/s10924-012-0422-9 . .