TY  - JOUR
AU  - Dmitrović, Slavica
AU  - Dragicević, Milan
AU  - Savić, Jelena
AU  - Milutinović, Milica
AU  - Živković, Suzana
AU  - Maksimović, Vuk
AU  - Matekalo, Dragana
AU  - Perisic, Mirjana
AU  - Misic, Danijela
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1398
AB  - Phosphinothricin (PPT) is one of the most widely used herbicides. PTT targets glutamine synthetase (GS) activity in plants, and its phytotoxicity is ascribed to ammonium accumulation and reactive oxygen species bursts, which drives rapid lipid peroxidation of cell membranes. In agricultural fields, PPT is extensively sprayed on plant foliage; however, a portion of the herbicide reaches the soil. According to the present study, PPT absorbed via roots can be phytotoxic to Arabidopsis, inducing more adverse effects in roots than in shoots. Alterations in plant physiology caused by 10 days exposure to herbicide via roots are reflected through growth suppression, reduced chlorophyll content, perturbations in the sugar and organic acid metabolism, modifications in the activities and abundances of GS, catalase, peroxidase, and superoxide dismutase. Antagonistic interaction of Nepeta rtanjensis essential oil (NrEO) and PPT, emphasizes the existence of complex control mechanisms at the transcriptional and posttranslational level, which result in the mitigation of PPT-induced ammonium toxicity and in providing more efficient antioxidant defense of plants. Simultaneous application of the two agents in the field cannot be recommended; however, NrEO might be considered as the PPT post-treatment for reducing harmful effects of herbicide residues in the soil on non-target plants.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Antagonistic Interaction between Phosphinothricin and Nepeta rtanjensis Essential Oil Affected Ammonium Metabolism and Antioxidant Defense of Arabidopsis Grown In Vitro
IS  - 1
VL  - 10
DO  - 10.3390/plants10010142
ER  - 

@article{
author = "Dmitrović, Slavica and Dragicević, Milan and Savić, Jelena and Milutinović, Milica and Živković, Suzana and Maksimović, Vuk and Matekalo, Dragana and Perisic, Mirjana and Misic, Danijela",
year = "2021",
abstract = "Phosphinothricin (PPT) is one of the most widely used herbicides. PTT targets glutamine synthetase (GS) activity in plants, and its phytotoxicity is ascribed to ammonium accumulation and reactive oxygen species bursts, which drives rapid lipid peroxidation of cell membranes. In agricultural fields, PPT is extensively sprayed on plant foliage; however, a portion of the herbicide reaches the soil. According to the present study, PPT absorbed via roots can be phytotoxic to Arabidopsis, inducing more adverse effects in roots than in shoots. Alterations in plant physiology caused by 10 days exposure to herbicide via roots are reflected through growth suppression, reduced chlorophyll content, perturbations in the sugar and organic acid metabolism, modifications in the activities and abundances of GS, catalase, peroxidase, and superoxide dismutase. Antagonistic interaction of Nepeta rtanjensis essential oil (NrEO) and PPT, emphasizes the existence of complex control mechanisms at the transcriptional and posttranslational level, which result in the mitigation of PPT-induced ammonium toxicity and in providing more efficient antioxidant defense of plants. Simultaneous application of the two agents in the field cannot be recommended; however, NrEO might be considered as the PPT post-treatment for reducing harmful effects of herbicide residues in the soil on non-target plants.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Antagonistic Interaction between Phosphinothricin and Nepeta rtanjensis Essential Oil Affected Ammonium Metabolism and Antioxidant Defense of Arabidopsis Grown In Vitro",
number = "1",
volume = "10",
doi = "10.3390/plants10010142"
}

Dmitrović, S., Dragicević, M., Savić, J., Milutinović, M., Živković, S., Maksimović, V., Matekalo, D., Perisic, M.,& Misic, D.. (2021). Antagonistic Interaction between Phosphinothricin and Nepeta rtanjensis Essential Oil Affected Ammonium Metabolism and Antioxidant Defense of Arabidopsis Grown In Vitro. in Plants-Basel
MDPI, Basel., 10(1).
https://doi.org/10.3390/plants10010142

Dmitrović S, Dragicević M, Savić J, Milutinović M, Živković S, Maksimović V, Matekalo D, Perisic M, Misic D. Antagonistic Interaction between Phosphinothricin and Nepeta rtanjensis Essential Oil Affected Ammonium Metabolism and Antioxidant Defense of Arabidopsis Grown In Vitro. in Plants-Basel. 2021;10(1).
doi:10.3390/plants10010142 .

Dmitrović, Slavica, Dragicević, Milan, Savić, Jelena, Milutinović, Milica, Živković, Suzana, Maksimović, Vuk, Matekalo, Dragana, Perisic, Mirjana, Misic, Danijela, "Antagonistic Interaction between Phosphinothricin and Nepeta rtanjensis Essential Oil Affected Ammonium Metabolism and Antioxidant Defense of Arabidopsis Grown In Vitro" in Plants-Basel, 10, no. 1 (2021),
https://doi.org/10.3390/plants10010142 . .

TY  - JOUR
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Maksimović, Vuk
AU  - Ciric, Ana
AU  - Ivanov, Marija
AU  - Bozunović, Jelena
AU  - Banjanac, Tijana
AU  - Misic, Danijela
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1368
AB  - Extracts of the aerial (fronds) and underground parts (rhizome and roots) of Asplenium adiantum-nigrum L. and Asplenium ruta-muraria L. were analyzed for their phenolic composition and antioxidant properties (scavenging capacities against DPPH center dot and ABTS(center dot+)), and were comparatively assayed for antimicrobial and antibiofilm activity. Total of the 17 phenolic compounds, belonging to the classes of phenolic acids (hydroxybenzoic and hydroxycinnamic acids), flavonoids (flavan-3-ols, flavonols), and xanthones were identified and quantified using UHPLC/DAD/(-)HESI-MS/MS analysis. The present study shows that two analyzed ferns are significantly diverse in terms of their phenolic profile. Flavonoids considerably contribute to the total phenolic content of A. ruta-muraria, ranging from 47% in roots to around 75% in rhizome and fronds, while A. adiantum-nigrum preferably accumulates xanthones. Antioxidant activity of fern methanol extracts was ascribed to their phenolics content. Extracts of rhizomes of both Asplenium ferns exhibited the highest antimicrobial activity, while frond extracts were more efficient in inhibition of Candida albicans biofilm formation. The present study provides experimental evidence that methanol extracts from two analyzed Asplenium fern species exhibit strong antibacterial effect against Gram-negative bacteria which candidate them as a potentially useful natural antimicrobial agents.
PB  - Elsevier, Amsterdam
T2  - South African Journal of Botany
T1  - Antioxidant and antimicrobial activity of two Asplenium species
EP  - 187
SP  - 180
VL  - 132
DO  - 10.1016/j.sajb.2020.03.034
ER  - 

@article{
author = "Živković, Suzana and Milutinović, Milica and Maksimović, Vuk and Ciric, Ana and Ivanov, Marija and Bozunović, Jelena and Banjanac, Tijana and Misic, Danijela",
year = "2020",
abstract = "Extracts of the aerial (fronds) and underground parts (rhizome and roots) of Asplenium adiantum-nigrum L. and Asplenium ruta-muraria L. were analyzed for their phenolic composition and antioxidant properties (scavenging capacities against DPPH center dot and ABTS(center dot+)), and were comparatively assayed for antimicrobial and antibiofilm activity. Total of the 17 phenolic compounds, belonging to the classes of phenolic acids (hydroxybenzoic and hydroxycinnamic acids), flavonoids (flavan-3-ols, flavonols), and xanthones were identified and quantified using UHPLC/DAD/(-)HESI-MS/MS analysis. The present study shows that two analyzed ferns are significantly diverse in terms of their phenolic profile. Flavonoids considerably contribute to the total phenolic content of A. ruta-muraria, ranging from 47% in roots to around 75% in rhizome and fronds, while A. adiantum-nigrum preferably accumulates xanthones. Antioxidant activity of fern methanol extracts was ascribed to their phenolics content. Extracts of rhizomes of both Asplenium ferns exhibited the highest antimicrobial activity, while frond extracts were more efficient in inhibition of Candida albicans biofilm formation. The present study provides experimental evidence that methanol extracts from two analyzed Asplenium fern species exhibit strong antibacterial effect against Gram-negative bacteria which candidate them as a potentially useful natural antimicrobial agents.",
publisher = "Elsevier, Amsterdam",
journal = "South African Journal of Botany",
title = "Antioxidant and antimicrobial activity of two Asplenium species",
pages = "187-180",
volume = "132",
doi = "10.1016/j.sajb.2020.03.034"
}

Živković, S., Milutinović, M., Maksimović, V., Ciric, A., Ivanov, M., Bozunović, J., Banjanac, T.,& Misic, D.. (2020). Antioxidant and antimicrobial activity of two Asplenium species. in South African Journal of Botany
Elsevier, Amsterdam., 132, 180-187.
https://doi.org/10.1016/j.sajb.2020.03.034

Živković S, Milutinović M, Maksimović V, Ciric A, Ivanov M, Bozunović J, Banjanac T, Misic D. Antioxidant and antimicrobial activity of two Asplenium species. in South African Journal of Botany. 2020;132:180-187.
doi:10.1016/j.sajb.2020.03.034 .

Živković, Suzana, Milutinović, Milica, Maksimović, Vuk, Ciric, Ana, Ivanov, Marija, Bozunović, Jelena, Banjanac, Tijana, Misic, Danijela, "Antioxidant and antimicrobial activity of two Asplenium species" in South African Journal of Botany, 132 (2020):180-187,
https://doi.org/10.1016/j.sajb.2020.03.034 . .

TY  - JOUR
AU  - Dmitrović, Slavica
AU  - Dragicević, Milan
AU  - Savić, Jelena
AU  - Milutinović, Milica
AU  - Živković, Suzana
AU  - Maksimović, Vuk
AU  - Matekalo, Dragana
AU  - Misic, Danijela
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1244
AB  - Active ingredient of the commercial herbicide BASTA (B), phosphinothricin, acts as an inhibitor of glutamine synthetase (GS), a key enzyme in ammonium assimilation. The treatment with BASTA leads to an elevation of ammonium levels in plants and further to various physiological alterations, ammonium toxicity and lethality. Results of the present study emphasize the complexity underlying control mechanisms that determine BASTA interaction with essential oil (EO) from Nepeta rtanjensis (NrEO), bioherbicide inducing oxidative stress in target plants. Simultaneous application of NrEO and BASTA, two agents showing differential mode of action, suspends BASTA-induced ammonium toxicity in Arabidopsis thaliana plants. This is achieved through maintaining GS activity, which sustains a sub-toxic and/or sub-lethal ammonium concentration in tissues. As revealed by the present study, regulation of GS activity, as influenced by BASTA and NrEO, occurs at transcriptional, posttranscriptional, and/or posttranslational levels. Two genes encoding cytosolic GS, GLN1;1 and GLN1;3, are highlighted as the main isozymes in Arabidopsis shoots contributing to NrEO-induced overcoming of BASTA-generated ammonium toxicity. The effects of NrEO might be ascribed to its major component nepetalactone, but the contribution of minor EO components should not be neglected. Although of fundamental significance, the results of the present study suggest possible low efficiency of BASTA in plantations of medicinal/aromatic plants such as Nepeta species. Furthermore, these results highlight the possibility of using NrEO as a bioherbicide in BASTA-treated crop fields to mitigate the effect of BASTA residues in contaminated soils.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Plant Physiology
T1  - Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.
EP  - 94
SP  - 87
VL  - 237
DO  - 10.1016/j.jplph.2019.04.006
ER  - 

@article{
author = "Dmitrović, Slavica and Dragicević, Milan and Savić, Jelena and Milutinović, Milica and Živković, Suzana and Maksimović, Vuk and Matekalo, Dragana and Misic, Danijela",
year = "2019",
abstract = "Active ingredient of the commercial herbicide BASTA (B), phosphinothricin, acts as an inhibitor of glutamine synthetase (GS), a key enzyme in ammonium assimilation. The treatment with BASTA leads to an elevation of ammonium levels in plants and further to various physiological alterations, ammonium toxicity and lethality. Results of the present study emphasize the complexity underlying control mechanisms that determine BASTA interaction with essential oil (EO) from Nepeta rtanjensis (NrEO), bioherbicide inducing oxidative stress in target plants. Simultaneous application of NrEO and BASTA, two agents showing differential mode of action, suspends BASTA-induced ammonium toxicity in Arabidopsis thaliana plants. This is achieved through maintaining GS activity, which sustains a sub-toxic and/or sub-lethal ammonium concentration in tissues. As revealed by the present study, regulation of GS activity, as influenced by BASTA and NrEO, occurs at transcriptional, posttranscriptional, and/or posttranslational levels. Two genes encoding cytosolic GS, GLN1;1 and GLN1;3, are highlighted as the main isozymes in Arabidopsis shoots contributing to NrEO-induced overcoming of BASTA-generated ammonium toxicity. The effects of NrEO might be ascribed to its major component nepetalactone, but the contribution of minor EO components should not be neglected. Although of fundamental significance, the results of the present study suggest possible low efficiency of BASTA in plantations of medicinal/aromatic plants such as Nepeta species. Furthermore, these results highlight the possibility of using NrEO as a bioherbicide in BASTA-treated crop fields to mitigate the effect of BASTA residues in contaminated soils.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Plant Physiology",
title = "Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.",
pages = "94-87",
volume = "237",
doi = "10.1016/j.jplph.2019.04.006"
}

Dmitrović, S., Dragicević, M., Savić, J., Milutinović, M., Živković, S., Maksimović, V., Matekalo, D.,& Misic, D.. (2019). Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.. in Journal of Plant Physiology
Elsevier Gmbh, Munich., 237, 87-94.
https://doi.org/10.1016/j.jplph.2019.04.006

Dmitrović S, Dragicević M, Savić J, Milutinović M, Živković S, Maksimović V, Matekalo D, Misic D. Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.. in Journal of Plant Physiology. 2019;237:87-94.
doi:10.1016/j.jplph.2019.04.006 .

Dmitrović, Slavica, Dragicević, Milan, Savić, Jelena, Milutinović, Milica, Živković, Suzana, Maksimović, Vuk, Matekalo, Dragana, Misic, Danijela, "Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh." in Journal of Plant Physiology, 237 (2019):87-94,
https://doi.org/10.1016/j.jplph.2019.04.006 . .

TY  - JOUR
AU  - Mitic, Nevena V
AU  - Dmitrović, Slavica
AU  - Đorđević, Mirka
AU  - Zdravkovic-Korac, Snežana R
AU  - Nikolic, Radomirka R
AU  - Raspor, Martin
AU  - Đorđević, Tatjana
AU  - Maksimović, Vuk
AU  - Živković, Suzana
AU  - Krstić-Milošević, Dijana B.
AU  - Stanisic, Mariana
AU  - Ninković, Slavica
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/599
AB  - We investigated Chenopodium murale transgenic hairy root in vitro culture system as a new tool for allelopathic assays. Transgenic hairy roots were induced by Agrobacterium rhizogenes A4M70GUS from roots, cotyledons, leaves, and internodes of C. murale seedlings. Roots were found to be the best target explants, providing transformation efficiency of up to 11.1%. Established hairy root clones differed in their morphology and growth potential. Molecular characterization of these clones was carried out by PCR, RT-PCR and histochemical GUS analyses. No differences in rol gene expression were observed. Liquid culture system of characterized hairy root clones was maintained for over 2 years. Six hairy root clones were selected for assaying the allelopathic effect of their growth medium against germination and seedling elongation of wheat and lettuce test plants. The inhibitory potential varied depending on the hairy root clone. Some transgenic clones showed significantly higher inhibition compared to wild-type roots. These results revealed that hairy roots as an independent system synthesize some bioactive substances with allelopathic activity and exude them into the growth medium. Concentrations of caffeic, ferulic and p-coumaric acids (0.07-2.85 mu mol/L) identified by HPLC analysis in the growth media were at least 1000 times lower than the inhibitory active concentration (5 mmol/L) of pure grade phenolic acids, suggesting that they have a limited role in the allelopathic phenomena of C murale. The presented hairy root system appears to be a suitable tool for further investigation of the potential and nature of root-mediated allelopathic interference of C. murale.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Plant Physiology
T1  - Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays
EP  - 1211
IS  - 12
SP  - 1203
VL  - 169
DO  - 10.1016/j.jplph.2012.04.009
ER  - 

@article{
author = "Mitic, Nevena V and Dmitrović, Slavica and Đorđević, Mirka and Zdravkovic-Korac, Snežana R and Nikolic, Radomirka R and Raspor, Martin and Đorđević, Tatjana and Maksimović, Vuk and Živković, Suzana and Krstić-Milošević, Dijana B. and Stanisic, Mariana and Ninković, Slavica",
year = "2012",
abstract = "We investigated Chenopodium murale transgenic hairy root in vitro culture system as a new tool for allelopathic assays. Transgenic hairy roots were induced by Agrobacterium rhizogenes A4M70GUS from roots, cotyledons, leaves, and internodes of C. murale seedlings. Roots were found to be the best target explants, providing transformation efficiency of up to 11.1%. Established hairy root clones differed in their morphology and growth potential. Molecular characterization of these clones was carried out by PCR, RT-PCR and histochemical GUS analyses. No differences in rol gene expression were observed. Liquid culture system of characterized hairy root clones was maintained for over 2 years. Six hairy root clones were selected for assaying the allelopathic effect of their growth medium against germination and seedling elongation of wheat and lettuce test plants. The inhibitory potential varied depending on the hairy root clone. Some transgenic clones showed significantly higher inhibition compared to wild-type roots. These results revealed that hairy roots as an independent system synthesize some bioactive substances with allelopathic activity and exude them into the growth medium. Concentrations of caffeic, ferulic and p-coumaric acids (0.07-2.85 mu mol/L) identified by HPLC analysis in the growth media were at least 1000 times lower than the inhibitory active concentration (5 mmol/L) of pure grade phenolic acids, suggesting that they have a limited role in the allelopathic phenomena of C murale. The presented hairy root system appears to be a suitable tool for further investigation of the potential and nature of root-mediated allelopathic interference of C. murale.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Plant Physiology",
title = "Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays",
pages = "1211-1203",
number = "12",
volume = "169",
doi = "10.1016/j.jplph.2012.04.009"
}

Mitic, N. V., Dmitrović, S., Đorđević, M., Zdravkovic-Korac, S. R., Nikolic, R. R., Raspor, M., Đorđević, T., Maksimović, V., Živković, S., Krstić-Milošević, D. B., Stanisic, M.,& Ninković, S.. (2012). Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays. in Journal of Plant Physiology
Elsevier Gmbh, Munich., 169(12), 1203-1211.
https://doi.org/10.1016/j.jplph.2012.04.009

Mitic NV, Dmitrović S, Đorđević M, Zdravkovic-Korac SR, Nikolic RR, Raspor M, Đorđević T, Maksimović V, Živković S, Krstić-Milošević DB, Stanisic M, Ninković S. Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays. in Journal of Plant Physiology. 2012;169(12):1203-1211.
doi:10.1016/j.jplph.2012.04.009 .

Mitic, Nevena V, Dmitrović, Slavica, Đorđević, Mirka, Zdravkovic-Korac, Snežana R, Nikolic, Radomirka R, Raspor, Martin, Đorđević, Tatjana, Maksimović, Vuk, Živković, Suzana, Krstić-Milošević, Dijana B., Stanisic, Mariana, Ninković, Slavica, "Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays" in Journal of Plant Physiology, 169, no. 12 (2012):1203-1211,
https://doi.org/10.1016/j.jplph.2012.04.009 . .

TY  - JOUR
AU  - Misic, Danijela
AU  - Siler, Branislav
AU  - Nestorović-Živković, Jasmina M
AU  - Simonović, Ana D
AU  - Maksimović, Vuk
AU  - Budimir, Snežana M
AU  - Janosević, Dusica A
AU  - Djuricković, Milutin S
AU  - Nikolic, Miroslav
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/506
AB  - The effect of reduced availability of sugars on growth and essential metabolic processes in roots, resulting from decreased photosynthesis under salinity, was excluded by establishing a non-photosynthetic model-system in this study: root cultures of Centaurium maritimum (L.) Fritch and Centaurium spicatum (L.) Fritch. The contribution of inorganic cations and organic compounds (e. g. carbohydrates and amino acids) to the osmotic adjustment (OA) in roots during short-term exposure to various salt concentrations (0, 50, 100 or 200 mM NaCl) was emphasized. Observed morphological and histological changes in roots were species specific, and were dependent on salinity level. Although C. spicatum appears to be more tolerant to salt stress, both species employed similar strategies in response to elevated salinity to different extents, and displayed effective OA mechanisms. Under low and moderate salinity, inorganic cations were the major contributors to OA in roots of both species, followed by soluble sugars, while the relative contribution of proline (Pro) and free amino acids was insignificant. Osmotic adjustment under severe stress appears to be mediated by increased accumulation of organic compounds. The analysis of the intraspecies variability in salt response of C. spicatum and C. maritimum roots enabled the identification of some organic compounds which could be used as potential biochemical markers in screening for salt tolerance, including Pro in C. spicatum, and trehalose and polyols in C. maritimum.
PB  - Springer, Dordrecht
T2  - Plant Cell Tissue and Organ Culture
T1  - Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress
EP  - 400
IS  - 3
SP  - 389
VL  - 108
DO  - 10.1007/s11240-011-0050-4
ER  - 

@article{
author = "Misic, Danijela and Siler, Branislav and Nestorović-Živković, Jasmina M and Simonović, Ana D and Maksimović, Vuk and Budimir, Snežana M and Janosević, Dusica A and Djuricković, Milutin S and Nikolic, Miroslav",
year = "2012",
abstract = "The effect of reduced availability of sugars on growth and essential metabolic processes in roots, resulting from decreased photosynthesis under salinity, was excluded by establishing a non-photosynthetic model-system in this study: root cultures of Centaurium maritimum (L.) Fritch and Centaurium spicatum (L.) Fritch. The contribution of inorganic cations and organic compounds (e. g. carbohydrates and amino acids) to the osmotic adjustment (OA) in roots during short-term exposure to various salt concentrations (0, 50, 100 or 200 mM NaCl) was emphasized. Observed morphological and histological changes in roots were species specific, and were dependent on salinity level. Although C. spicatum appears to be more tolerant to salt stress, both species employed similar strategies in response to elevated salinity to different extents, and displayed effective OA mechanisms. Under low and moderate salinity, inorganic cations were the major contributors to OA in roots of both species, followed by soluble sugars, while the relative contribution of proline (Pro) and free amino acids was insignificant. Osmotic adjustment under severe stress appears to be mediated by increased accumulation of organic compounds. The analysis of the intraspecies variability in salt response of C. spicatum and C. maritimum roots enabled the identification of some organic compounds which could be used as potential biochemical markers in screening for salt tolerance, including Pro in C. spicatum, and trehalose and polyols in C. maritimum.",
publisher = "Springer, Dordrecht",
journal = "Plant Cell Tissue and Organ Culture",
title = "Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress",
pages = "400-389",
number = "3",
volume = "108",
doi = "10.1007/s11240-011-0050-4"
}

Misic, D., Siler, B., Nestorović-Živković, J. M., Simonović, A. D., Maksimović, V., Budimir, S. M., Janosević, D. A., Djuricković, M. S.,& Nikolic, M.. (2012). Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress. in Plant Cell Tissue and Organ Culture
Springer, Dordrecht., 108(3), 389-400.
https://doi.org/10.1007/s11240-011-0050-4

Misic D, Siler B, Nestorović-Živković JM, Simonović AD, Maksimović V, Budimir SM, Janosević DA, Djuricković MS, Nikolic M. Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress. in Plant Cell Tissue and Organ Culture. 2012;108(3):389-400.
doi:10.1007/s11240-011-0050-4 .

Misic, Danijela, Siler, Branislav, Nestorović-Živković, Jasmina M, Simonović, Ana D, Maksimović, Vuk, Budimir, Snežana M, Janosević, Dusica A, Djuricković, Milutin S, Nikolic, Miroslav, "Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress" in Plant Cell Tissue and Organ Culture, 108, no. 3 (2012):389-400,
https://doi.org/10.1007/s11240-011-0050-4 . .

TY  - JOUR
AU  - Misic, Danijela
AU  - Dragicević, Milan
AU  - Siler, Branislav
AU  - Nestorović-Živković, Jasmina M
AU  - Maksimović, Vuk
AU  - Momcilović, Ivana
AU  - Nikolic, Miroslav
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/542
AB  - A heterotrophic model system was established in our studies in order to differentiate the effect of high salt concentrations in external medium on growth and sugar metabolism in roots from the effect of reduced sugar availability resulting from decreased photosynthesis under salinity. Soluble sugar content and the activity of acid invertase in root cultures of salt-tolerant (ST) and salt-sensitive (SS) Schenkia spicata (L.) Mansion genotypes were investigated during exposure to different NaCI concentrations (0-200 mM). Their response to severe salinity was characterized by a metabolic adjustment that led to the accumulation of sucrose (Suc) in root tissues. There was clear evidence that cell wall invertase (CW-Inv) is, the major contributor to the Suc/hexose ratio in roots during exposure to elevated salinity. The results of CW-Inv activity and immunodetection assays in our study suggest that the regulation of CW-Inv expression is most likely achieved in a salt stress dependent manner. Also, NaCI modulated soluble acid invertase (SA-Inv) expression differentially in SS and ST genotypes of S. spicata. Regardless of the salt treatment, genotype, or the amount of enzyme, SA-Inv activity was generally low, indicating regulation at the posttranslational level. The results suggest no direct role of SA-Inv in the regulation of the root tissue carbohydrate pool and therefore in the control of the availability of glucose and fructose for the primary metabolism and/or osmotic adjustment in the present heterotrophic model system, 2012 Elsevier GmbH. All rights reserved.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Journal of Plant Physiology
T1  - Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress
EP  - 1289
IS  - 13
SP  - 1281
VL  - 169
DO  - 10.1016/j.jplph.2012.04.018
ER  - 

@article{
author = "Misic, Danijela and Dragicević, Milan and Siler, Branislav and Nestorović-Živković, Jasmina M and Maksimović, Vuk and Momcilović, Ivana and Nikolic, Miroslav",
year = "2012",
abstract = "A heterotrophic model system was established in our studies in order to differentiate the effect of high salt concentrations in external medium on growth and sugar metabolism in roots from the effect of reduced sugar availability resulting from decreased photosynthesis under salinity. Soluble sugar content and the activity of acid invertase in root cultures of salt-tolerant (ST) and salt-sensitive (SS) Schenkia spicata (L.) Mansion genotypes were investigated during exposure to different NaCI concentrations (0-200 mM). Their response to severe salinity was characterized by a metabolic adjustment that led to the accumulation of sucrose (Suc) in root tissues. There was clear evidence that cell wall invertase (CW-Inv) is, the major contributor to the Suc/hexose ratio in roots during exposure to elevated salinity. The results of CW-Inv activity and immunodetection assays in our study suggest that the regulation of CW-Inv expression is most likely achieved in a salt stress dependent manner. Also, NaCI modulated soluble acid invertase (SA-Inv) expression differentially in SS and ST genotypes of S. spicata. Regardless of the salt treatment, genotype, or the amount of enzyme, SA-Inv activity was generally low, indicating regulation at the posttranslational level. The results suggest no direct role of SA-Inv in the regulation of the root tissue carbohydrate pool and therefore in the control of the availability of glucose and fructose for the primary metabolism and/or osmotic adjustment in the present heterotrophic model system, 2012 Elsevier GmbH. All rights reserved.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Journal of Plant Physiology",
title = "Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress",
pages = "1289-1281",
number = "13",
volume = "169",
doi = "10.1016/j.jplph.2012.04.018"
}

Misic, D., Dragicević, M., Siler, B., Nestorović-Živković, J. M., Maksimović, V., Momcilović, I.,& Nikolic, M.. (2012). Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress. in Journal of Plant Physiology
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 169(13), 1281-1289.
https://doi.org/10.1016/j.jplph.2012.04.018

Misic D, Dragicević M, Siler B, Nestorović-Živković JM, Maksimović V, Momcilović I, Nikolic M. Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress. in Journal of Plant Physiology. 2012;169(13):1281-1289.
doi:10.1016/j.jplph.2012.04.018 .

Misic, Danijela, Dragicević, Milan, Siler, Branislav, Nestorović-Živković, Jasmina M, Maksimović, Vuk, Momcilović, Ivana, Nikolic, Miroslav, "Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress" in Journal of Plant Physiology, 169, no. 13 (2012):1281-1289,
https://doi.org/10.1016/j.jplph.2012.04.018 . .