TY  - JOUR
AU  - Ducic, Tanja
AU  - Borchert, Manuela
AU  - Savić, Aleksandar G
AU  - Kalauzi, Aleksandar
AU  - Mitrović, Aleksandra Lj.
AU  - Radotić, Ksenija
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/696
AB  - Synchrotron-based X-ray microfluorescence (mu SXRF) is an analytical method suitable for in situ investigation of the distribution of micronutrient and macronutrient elements in several-micrometres-thick unstained biological samples, e. g. single cells and tissues. Elements are mapped and quantified at sub-p. p. m. concentrations. In this study the quantity, distribution and grouping/co-localization of various elements have been identified in straight and twisted internodes of the stems of the monocotyledonous climber D. balcanica Kosanin. Three different statistical methods were employed to analyse the macronutrient and micronutrient distributions and co-localization. Macronutrient elements (K, P, Ca, Cl) are distributed homogeneously in both straight and twisted internodes. Micronutrient elements are mostly grouped in the vasculature and in the sclerenchyma cell layer. In addition, co-localization of micronutrient elements is much more prominent in twisted than in straight internodes. These image analyses and statistical methods provided very similar outcomes and could be applied to various types of biological samples imaged by mSXRF.
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of Synchrotron Radiation
T1  - Enhancement in statistical and image analysis for in situ mu SXRF studies of elemental distribution and co-localization, using Dioscorea balcanica
EP  - 346
SP  - 339
VL  - 20
DO  - 10.1107/S0909049512050170
ER  - 

@article{
author = "Ducic, Tanja and Borchert, Manuela and Savić, Aleksandar G and Kalauzi, Aleksandar and Mitrović, Aleksandra Lj. and Radotić, Ksenija",
year = "2013",
abstract = "Synchrotron-based X-ray microfluorescence (mu SXRF) is an analytical method suitable for in situ investigation of the distribution of micronutrient and macronutrient elements in several-micrometres-thick unstained biological samples, e. g. single cells and tissues. Elements are mapped and quantified at sub-p. p. m. concentrations. In this study the quantity, distribution and grouping/co-localization of various elements have been identified in straight and twisted internodes of the stems of the monocotyledonous climber D. balcanica Kosanin. Three different statistical methods were employed to analyse the macronutrient and micronutrient distributions and co-localization. Macronutrient elements (K, P, Ca, Cl) are distributed homogeneously in both straight and twisted internodes. Micronutrient elements are mostly grouped in the vasculature and in the sclerenchyma cell layer. In addition, co-localization of micronutrient elements is much more prominent in twisted than in straight internodes. These image analyses and statistical methods provided very similar outcomes and could be applied to various types of biological samples imaged by mSXRF.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of Synchrotron Radiation",
title = "Enhancement in statistical and image analysis for in situ mu SXRF studies of elemental distribution and co-localization, using Dioscorea balcanica",
pages = "346-339",
volume = "20",
doi = "10.1107/S0909049512050170"
}

Ducic, T., Borchert, M., Savić, A. G., Kalauzi, A., Mitrović, A. Lj.,& Radotić, K.. (2013). Enhancement in statistical and image analysis for in situ mu SXRF studies of elemental distribution and co-localization, using Dioscorea balcanica. in Journal of Synchrotron Radiation
Wiley-Blackwell, Hoboken., 20, 339-346.
https://doi.org/10.1107/S0909049512050170

Ducic T, Borchert M, Savić AG, Kalauzi A, Mitrović AL, Radotić K. Enhancement in statistical and image analysis for in situ mu SXRF studies of elemental distribution and co-localization, using Dioscorea balcanica. in Journal of Synchrotron Radiation. 2013;20:339-346.
doi:10.1107/S0909049512050170 .

Ducic, Tanja, Borchert, Manuela, Savić, Aleksandar G, Kalauzi, Aleksandar, Mitrović, Aleksandra Lj., Radotić, Ksenija, "Enhancement in statistical and image analysis for in situ mu SXRF studies of elemental distribution and co-localization, using Dioscorea balcanica" in Journal of Synchrotron Radiation, 20 (2013):339-346,
https://doi.org/10.1107/S0909049512050170 . .