TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Aborgiba, Mustafa
AU  - Gacic, Zoran
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1927
AB  - Due to the presence of a large number of different pollutants, monitoring of the surface water quality based solely on the analysis of a limited number of xenobiotics, cannot be considered as reliable. Beside toxic, these agents can exert genotoxic effects, inducing damage in the DNA molecule, which, if not repaired, could lead to mutations and alterations in cells, tissues, organism, whole population and the ecosystem. The surface waters are under the pressure of both anthropogenic and natural sources of pollution. Additionally, extreme hydrological events, such as water scarcity and flooding, may further impair the state of freshwater bodies. Fish may be exposed to harmful substances through water, sediment and food. In ecogenotoxicity studies, gills are used as they represent the first organ in direct contact with water and waterborne pollutants, while the liver, as a key organ that controls many life functions is used as a major organ for metabolic breakdown of xenobiotics. Common bream (Abramis brama), white bream (Blicca bjoerkna) and white-eye bream (Ballerus sapa) are three closely related, benthivorous cyprinids, native for the Sava River. The sampling site Duboko (23 rkm), on the Sava River, is chosen as it is exposed to the untreated wastewater from the town of Obrenovac (more than 70,000 inhabitants), intensive agricultural activity and close proximity to the largest thermal power plant in Serbia (TENTA) and belonging ash field. This study was conducted to assess the impact of multiple stressors during different seasons on different levels of biological organization, subcellular (genotoxic effect) and cellular/tissue level (histopathological effects), in the liver and gills of three bream species. As a biomarker of exposure DNA damage was measured by applying the alkaline comet assay, while histopathological alterations were monitored as a biomarker of effect. In parallel, concentration of metals and metalloids were assessed in gills, liver and muscle.
Basic physical (pH, temperature, oxygen concentration, electrical conductivity) and chemical (NO2, NO3-, NH4+, PO43-) parameters were measured on site. Microbiological indicators of faecal pollution, total coliforms (TC), Escherichia coli (EC) and Enterococci (EF) were assessed by using a most probable number approach (MPN). Presumptive Clostridium perfringens (CP) numbers were determined by using membrane filtration and incubation on TSC (Tryptose Sulphite Cycloserine) media. Sampling of fish tissue for comet assay and histopathological analyses was performed in 2014, during winter (January and February), spring (March and early June), and summer (late June, July, and August), once per month, for a total of 52 specimens. Analysis of metals and metalloids was performed only on fish sampled in February, early June and in August, in total 15 specimens. In the mid May extreme hydrological events led to extensive flooding in the studied area. Comet assay was conducted on the liver and gill cells according to Kostić et al. Tail intensity, % of DNA in the comet tail (TI) was used to express the DNA damage level. Histopathological analyses included the use of conventional histological methods, staining of tissues sections using hematoxylin eosine differential staining, and examined under the microscope. The type and the extent of histological alterations were described by using a method developed by Bernet et al. Analysis of metals and metalloids was performed by inductively-coupled plasma optical emission spectrometry (ICP-OES), and included assessment of concentrations of 16 elements (Al, As, B, Ba, Cd, Co, Cr, Cu, Fe, Li, Mn, Mo, Ni, Pb, Sr and Zn). To compare the total metal content in different tissues and through different seasons metal pollution index (MPI) was calculated according to equation MPI = (cf1 x cf2 x cf3 x…cfn) 1/n, where cfn = concentration of the metal n in the sample.
Statistical analysis of data from the individual months showed the highest DNA damage in gill cells during early June (spring). Gill histopathological index (IG) did not show significant seasonal variations, however it was the lowest during winter, the highest in spring, and slightly decreased in summer. A possible cause of this incidence could be a withdrawal of water which took place in June, after the flooding event that occurred in the middle of May. In liver the highest DNA damage was observed during August. Histopathological index of liver showed significantly higher values in summer in comparison to spring. This could be prescribed to a higher metabolic rate of fish liver during warm seasons and also could be a consequence of processing a large quantities of xenobiotics introduced into the water column due to withdrawal of water after floods and sediment disturbance. According to the MPI, gills were under the highest pressure of metal pollution during spring and summer. Liver was under the highest pressure of metal pollution during winter, while the muscle was the least affected tissue during all three seasons.
Overall, gills as the first organ in direct contact with water showed a higher response in terms of DNA damage (molecular level), while the liver as the major organ for processing of xenobiotics both from water and food showed a higher degree of histopathological alterations in comparison to gills (tissue/organ level). Increased response of both biomarkers during spring and summer indicates a joint effect of the flooding event and seasonal changes of climate and hydrological parameters.
PB  - Institute for Water Quality, Resource and Waste Management TU Wien
C3  - International Conference River Basins 2017, Vienna, Austria
T1  - The impact of the Sava river pollution on biomarkers response in the liver and gills of three cyprinid species
SP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1927
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Aborgiba, Mustafa and Gacic, Zoran and Raskovic, Bozidar and Poleksic, Vesna and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2017",
abstract = "Due to the presence of a large number of different pollutants, monitoring of the surface water quality based solely on the analysis of a limited number of xenobiotics, cannot be considered as reliable. Beside toxic, these agents can exert genotoxic effects, inducing damage in the DNA molecule, which, if not repaired, could lead to mutations and alterations in cells, tissues, organism, whole population and the ecosystem. The surface waters are under the pressure of both anthropogenic and natural sources of pollution. Additionally, extreme hydrological events, such as water scarcity and flooding, may further impair the state of freshwater bodies. Fish may be exposed to harmful substances through water, sediment and food. In ecogenotoxicity studies, gills are used as they represent the first organ in direct contact with water and waterborne pollutants, while the liver, as a key organ that controls many life functions is used as a major organ for metabolic breakdown of xenobiotics. Common bream (Abramis brama), white bream (Blicca bjoerkna) and white-eye bream (Ballerus sapa) are three closely related, benthivorous cyprinids, native for the Sava River. The sampling site Duboko (23 rkm), on the Sava River, is chosen as it is exposed to the untreated wastewater from the town of Obrenovac (more than 70,000 inhabitants), intensive agricultural activity and close proximity to the largest thermal power plant in Serbia (TENTA) and belonging ash field. This study was conducted to assess the impact of multiple stressors during different seasons on different levels of biological organization, subcellular (genotoxic effect) and cellular/tissue level (histopathological effects), in the liver and gills of three bream species. As a biomarker of exposure DNA damage was measured by applying the alkaline comet assay, while histopathological alterations were monitored as a biomarker of effect. In parallel, concentration of metals and metalloids were assessed in gills, liver and muscle.
Basic physical (pH, temperature, oxygen concentration, electrical conductivity) and chemical (NO2, NO3-, NH4+, PO43-) parameters were measured on site. Microbiological indicators of faecal pollution, total coliforms (TC), Escherichia coli (EC) and Enterococci (EF) were assessed by using a most probable number approach (MPN). Presumptive Clostridium perfringens (CP) numbers were determined by using membrane filtration and incubation on TSC (Tryptose Sulphite Cycloserine) media. Sampling of fish tissue for comet assay and histopathological analyses was performed in 2014, during winter (January and February), spring (March and early June), and summer (late June, July, and August), once per month, for a total of 52 specimens. Analysis of metals and metalloids was performed only on fish sampled in February, early June and in August, in total 15 specimens. In the mid May extreme hydrological events led to extensive flooding in the studied area. Comet assay was conducted on the liver and gill cells according to Kostić et al. Tail intensity, % of DNA in the comet tail (TI) was used to express the DNA damage level. Histopathological analyses included the use of conventional histological methods, staining of tissues sections using hematoxylin eosine differential staining, and examined under the microscope. The type and the extent of histological alterations were described by using a method developed by Bernet et al. Analysis of metals and metalloids was performed by inductively-coupled plasma optical emission spectrometry (ICP-OES), and included assessment of concentrations of 16 elements (Al, As, B, Ba, Cd, Co, Cr, Cu, Fe, Li, Mn, Mo, Ni, Pb, Sr and Zn). To compare the total metal content in different tissues and through different seasons metal pollution index (MPI) was calculated according to equation MPI = (cf1 x cf2 x cf3 x…cfn) 1/n, where cfn = concentration of the metal n in the sample.
Statistical analysis of data from the individual months showed the highest DNA damage in gill cells during early June (spring). Gill histopathological index (IG) did not show significant seasonal variations, however it was the lowest during winter, the highest in spring, and slightly decreased in summer. A possible cause of this incidence could be a withdrawal of water which took place in June, after the flooding event that occurred in the middle of May. In liver the highest DNA damage was observed during August. Histopathological index of liver showed significantly higher values in summer in comparison to spring. This could be prescribed to a higher metabolic rate of fish liver during warm seasons and also could be a consequence of processing a large quantities of xenobiotics introduced into the water column due to withdrawal of water after floods and sediment disturbance. According to the MPI, gills were under the highest pressure of metal pollution during spring and summer. Liver was under the highest pressure of metal pollution during winter, while the muscle was the least affected tissue during all three seasons.
Overall, gills as the first organ in direct contact with water showed a higher response in terms of DNA damage (molecular level), while the liver as the major organ for processing of xenobiotics both from water and food showed a higher degree of histopathological alterations in comparison to gills (tissue/organ level). Increased response of both biomarkers during spring and summer indicates a joint effect of the flooding event and seasonal changes of climate and hydrological parameters.",
publisher = "Institute for Water Quality, Resource and Waste Management TU Wien",
journal = "International Conference River Basins 2017, Vienna, Austria",
title = "The impact of the Sava river pollution on biomarkers response in the liver and gills of three cyprinid species",
pages = "8",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1927"
}

Kostić-Vuković, J., Kolarević, S., Kračun-Kolarević, M., Aborgiba, M., Gacic, Z., Raskovic, B., Poleksic, V., Lenhardt, M.,& Vuković-Gačić, B.. (2017). The impact of the Sava river pollution on biomarkers response in the liver and gills of three cyprinid species. in International Conference River Basins 2017, Vienna, Austria
Institute for Water Quality, Resource and Waste Management TU Wien., 8.
https://hdl.handle.net/21.15107/rcub_rimsi_1927

Kostić-Vuković J, Kolarević S, Kračun-Kolarević M, Aborgiba M, Gacic Z, Raskovic B, Poleksic V, Lenhardt M, Vuković-Gačić B. The impact of the Sava river pollution on biomarkers response in the liver and gills of three cyprinid species. in International Conference River Basins 2017, Vienna, Austria. 2017;:8.
https://hdl.handle.net/21.15107/rcub_rimsi_1927 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Aborgiba, Mustafa, Gacic, Zoran, Raskovic, Bozidar, Poleksic, Vesna, Lenhardt, Mirjana, Vuković-Gačić, Branka, "The impact of the Sava river pollution on biomarkers response in the liver and gills of three cyprinid species" in International Conference River Basins 2017, Vienna, Austria (2017):8,
https://hdl.handle.net/21.15107/rcub_rimsi_1927 .

TY  - CONF
AU  - Kostić, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarevic, Margareta
AU  - Gacic, Zoran
AU  - Aborgiba, Mustafa
AU  - Farnleitner, Andreas
AU  - Reischer, Georg
AU  - Linke, Rita
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2654
AB  - Introduction. The contamination of water bodies by sewage or manure is generally determined by fecal indicator bacteria. Specific differentiation between sources of contamination is of particular importance, since the health risk to humans is usually considered higher from human, than from animal fecal contamination. Quantitative PCR (qPCR)-based assays for analysis of human- or animal-associated genetic Bacteroidetes fecal markers have gained increased popularity in the field of microbial source tracking (MST).
Aim. The aim of the present study was to assess the level and source of microbial pollution in the Sava River Basin (SRB).
Materials and methods. The sampling at the SRB was performed in September 2015, at 15 locations. Additional samples were collected from 4 wastewater outlets. Quantification of total coliforms, E. coli and enterococci was performed with Colilert Quanti-Tray 2000 and MPN approach. The human-associated BacHum and HF183II, the ruminant-associated BacR and the pig-associated Pig2Bac fecal markers were enumerated by quantitative PCR (qPCR).
Results. According to the concentrations of fecal indicator bacteria, the river samples were scattered within the classes I and II (slight to moderate pollution). According to all monitored indicators, samples collected at 3 wastewater outlets were excessively contaminated. The results of MST revealed the presence of human-associated fecal markers BacHum and HF183II at 13 locations. The ruminant-associated BacR and the pig-associated Pig-2-Bac markers were not detected. High correlation was observed between the standard fecal indicators and human associated fecal markers.
Conclusions. The results reveal human origin of fecal pollution in the SRB and indicates the urgent need for effective wastewater treatment plants in water management.
Acknowledgements. EU Seventh Framework Programme – GLOBAQUA (no. 603629-ENV-2013-6.2.1). Ministry of Education, Science and Technological Development of the Republic of Serbia projects no. 173045 and 173025, and the bilateral project Serbia and Austria: SER Ev. No. 451-03-01039/2015-09/33.
PB  - Balkan Society for Microbiology
C3  - 10th Balkan Congress of Microbiology, Microbiologia Balkanica’2017, Sofia , Bulgaria
T1  - Determination of the level and source of microbial pollution in the Sava River Basin
SP  - 379
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2654
ER  - 

@conference{
author = "Kostić, Jovana and Kolarević, Stoimir and Kracun-Kolarevic, Margareta and Gacic, Zoran and Aborgiba, Mustafa and Farnleitner, Andreas and Reischer, Georg and Linke, Rita and Paunović, Momir and Vuković-Gačić, Branka",
year = "2017",
abstract = "Introduction. The contamination of water bodies by sewage or manure is generally determined by fecal indicator bacteria. Specific differentiation between sources of contamination is of particular importance, since the health risk to humans is usually considered higher from human, than from animal fecal contamination. Quantitative PCR (qPCR)-based assays for analysis of human- or animal-associated genetic Bacteroidetes fecal markers have gained increased popularity in the field of microbial source tracking (MST).
Aim. The aim of the present study was to assess the level and source of microbial pollution in the Sava River Basin (SRB).
Materials and methods. The sampling at the SRB was performed in September 2015, at 15 locations. Additional samples were collected from 4 wastewater outlets. Quantification of total coliforms, E. coli and enterococci was performed with Colilert Quanti-Tray 2000 and MPN approach. The human-associated BacHum and HF183II, the ruminant-associated BacR and the pig-associated Pig2Bac fecal markers were enumerated by quantitative PCR (qPCR).
Results. According to the concentrations of fecal indicator bacteria, the river samples were scattered within the classes I and II (slight to moderate pollution). According to all monitored indicators, samples collected at 3 wastewater outlets were excessively contaminated. The results of MST revealed the presence of human-associated fecal markers BacHum and HF183II at 13 locations. The ruminant-associated BacR and the pig-associated Pig-2-Bac markers were not detected. High correlation was observed between the standard fecal indicators and human associated fecal markers.
Conclusions. The results reveal human origin of fecal pollution in the SRB and indicates the urgent need for effective wastewater treatment plants in water management.
Acknowledgements. EU Seventh Framework Programme – GLOBAQUA (no. 603629-ENV-2013-6.2.1). Ministry of Education, Science and Technological Development of the Republic of Serbia projects no. 173045 and 173025, and the bilateral project Serbia and Austria: SER Ev. No. 451-03-01039/2015-09/33.",
publisher = "Balkan Society for Microbiology",
journal = "10th Balkan Congress of Microbiology, Microbiologia Balkanica’2017, Sofia , Bulgaria",
title = "Determination of the level and source of microbial pollution in the Sava River Basin",
pages = "379",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2654"
}

Kostić, J., Kolarević, S., Kracun-Kolarevic, M., Gacic, Z., Aborgiba, M., Farnleitner, A., Reischer, G., Linke, R., Paunović, M.,& Vuković-Gačić, B.. (2017). Determination of the level and source of microbial pollution in the Sava River Basin. in 10th Balkan Congress of Microbiology, Microbiologia Balkanica’2017, Sofia , Bulgaria
Balkan Society for Microbiology., 379.
https://hdl.handle.net/21.15107/rcub_rimsi_2654

Kostić J, Kolarević S, Kracun-Kolarevic M, Gacic Z, Aborgiba M, Farnleitner A, Reischer G, Linke R, Paunović M, Vuković-Gačić B. Determination of the level and source of microbial pollution in the Sava River Basin. in 10th Balkan Congress of Microbiology, Microbiologia Balkanica’2017, Sofia , Bulgaria. 2017;:379.
https://hdl.handle.net/21.15107/rcub_rimsi_2654 .

Kostić, Jovana, Kolarević, Stoimir, Kracun-Kolarevic, Margareta, Gacic, Zoran, Aborgiba, Mustafa, Farnleitner, Andreas, Reischer, Georg, Linke, Rita, Paunović, Momir, Vuković-Gačić, Branka, "Determination of the level and source of microbial pollution in the Sava River Basin" in 10th Balkan Congress of Microbiology, Microbiologia Balkanica’2017, Sofia , Bulgaria (2017):379,
https://hdl.handle.net/21.15107/rcub_rimsi_2654 .

TY  - JOUR
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Aborgiba, Mustafa
AU  - Gačić, Zoran
AU  - Paunović, Momir
AU  - Višnjić Jeftić, Željka
AU  - Rasković, Božidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1060
AB  - Biomarkers attract increasing attention in environmental studies, as a tool for detection of exposure and effects of pollution, from both natural and anthropogenic sources. This study aims to assess the impact of multiple stressors during distinctive seasons, covering also extreme hydrological events (extensive flooding in the mid May 2014), on different levels of biological organization in the liver and gills of three closely related freshwater breams. Our previous study on DNA damage in blood cells of these specimens showed increased DNA damage in June 2014, one month after the flooding event. As a continuation of that research, the present study was conducted. As a biomarker of exposure DNA damage was measured by applying the alkaline comet assay, while histopathological alterations were monitored as a biomarker of effect. Additionally, concentrations of metals and metalloids in gills, liver and muscle were assessed. Sampling of fish tissues was performed in 2014, during winter (January and February), spring (March and early June) and summer (late June, July and August). Significant seasonal difference in DNA damage was observed for both tissues. During spring and summer the level of DNA damage in gills was significantly higher when compared to the liver. Histopathological analyses showed higher frequency of alterations in gills during spring, and in liver during summer, but without a significant seasonal difference. Gills had the highest concentration of metals and metalloids during the spring and summer, and liver duringwinter. Muscle was the least affected tissue during all three seasons. This study highlighted the importance of the multiple biomarker approach and the use of different fish tissues in assessment of surface water pollution.
PB  - Elsevier Science Bv, Amsterdam
T2  - Science of the Total Environment
T1  - The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons
EP  - 1681
SP  - 1670
VL  - 601
DO  - 10.1016/j.scitotenv.2017.05.273
ER  - 

@article{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Aborgiba, Mustafa and Gačić, Zoran and Paunović, Momir and Višnjić Jeftić, Željka and Rasković, Božidar and Poleksic, Vesna and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2017",
abstract = "Biomarkers attract increasing attention in environmental studies, as a tool for detection of exposure and effects of pollution, from both natural and anthropogenic sources. This study aims to assess the impact of multiple stressors during distinctive seasons, covering also extreme hydrological events (extensive flooding in the mid May 2014), on different levels of biological organization in the liver and gills of three closely related freshwater breams. Our previous study on DNA damage in blood cells of these specimens showed increased DNA damage in June 2014, one month after the flooding event. As a continuation of that research, the present study was conducted. As a biomarker of exposure DNA damage was measured by applying the alkaline comet assay, while histopathological alterations were monitored as a biomarker of effect. Additionally, concentrations of metals and metalloids in gills, liver and muscle were assessed. Sampling of fish tissues was performed in 2014, during winter (January and February), spring (March and early June) and summer (late June, July and August). Significant seasonal difference in DNA damage was observed for both tissues. During spring and summer the level of DNA damage in gills was significantly higher when compared to the liver. Histopathological analyses showed higher frequency of alterations in gills during spring, and in liver during summer, but without a significant seasonal difference. Gills had the highest concentration of metals and metalloids during the spring and summer, and liver duringwinter. Muscle was the least affected tissue during all three seasons. This study highlighted the importance of the multiple biomarker approach and the use of different fish tissues in assessment of surface water pollution.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Science of the Total Environment",
title = "The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons",
pages = "1681-1670",
volume = "601",
doi = "10.1016/j.scitotenv.2017.05.273"
}

Kostić-Vuković, J., Kolarević, S., Kracun-Kolarević, M., Aborgiba, M., Gačić, Z., Paunović, M., Višnjić Jeftić, Ž., Rasković, B., Poleksic, V., Lenhardt, M.,& Vukovic-Gacic, B.. (2017). The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons. in Science of the Total Environment
Elsevier Science Bv, Amsterdam., 601, 1670-1681.
https://doi.org/10.1016/j.scitotenv.2017.05.273

Kostić-Vuković J, Kolarević S, Kracun-Kolarević M, Aborgiba M, Gačić Z, Paunović M, Višnjić Jeftić Ž, Rasković B, Poleksic V, Lenhardt M, Vukovic-Gacic B. The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons. in Science of the Total Environment. 2017;601:1670-1681.
doi:10.1016/j.scitotenv.2017.05.273 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Aborgiba, Mustafa, Gačić, Zoran, Paunović, Momir, Višnjić Jeftić, Željka, Rasković, Božidar, Poleksic, Vesna, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons" in Science of the Total Environment, 601 (2017):1670-1681,
https://doi.org/10.1016/j.scitotenv.2017.05.273 . .

TY  - CONF
AU  - Kostić, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarevic, Margareta
AU  - Aborgiba, Mustafa
AU  - Gacic, Zoran
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2760
AB  - Aquatic ecosystems constant deterioration requires regular monitoring of the genotoxic potential since unrepaired changes in DNA molecule of aquatic organisms may cause consequences on various levels of biological organisation. Fish are one of the most frequently used groups of bioindicator organisms in ecotoxicological field studies. The seasonal changes in genotoxic potential of the Danube River were assessed by comet assay on three tissues of freshwater bream Abramis brama, which is a wide spread, autochthonous species in this river, often used in human nutrition. For assessment of DNA damage we used Comet assay (single cell gel electrophoresis) SCGE. The study was carried out on the site Visnjica, situated downstream Belgrade (Serbian capitol) which is identified as one of the major hotspots of faecal pollution along the Danube River. Basic physico-chemical and microbiological parameters of water quality were monitored along with the sampling of fish blood, liver and gills in February, April, August and November 2014. Comets were randomly scored and analyzed using Comet IV computer software (Perceptive Instruments, UK). DNA damage level is expressed using Tail Intensity (TI). All three tissues had highest level of DNA damage in August, which was month with the highest water temperature. Blood had the lowest DNA damage level in November, gills in April and liver in February. Similar response in all tissues was noticed with slight variations which is expected considering the differences in the level of exposure and metabolism in the selected tissues. Freshwater bream is shown to be a potentially good indicator organism in field studies of genotoxic potential.
PB  - US National Institute of Environmental Health Science, US Environmental Protection Agency
C3  - The Central & Eastern Europe Conference on Health and the Environment (CEECHE), Prague, Czech Republic
T1  - Seasonal changes in genotoxic potential of the Danube river assessed by comet assay on tissues of freshwater bream
SP  - 151
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2760
ER  - 

@conference{
author = "Kostić, Jovana and Kolarević, Stoimir and Kracun-Kolarevic, Margareta and Aborgiba, Mustafa and Gacic, Zoran and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2016",
abstract = "Aquatic ecosystems constant deterioration requires regular monitoring of the genotoxic potential since unrepaired changes in DNA molecule of aquatic organisms may cause consequences on various levels of biological organisation. Fish are one of the most frequently used groups of bioindicator organisms in ecotoxicological field studies. The seasonal changes in genotoxic potential of the Danube River were assessed by comet assay on three tissues of freshwater bream Abramis brama, which is a wide spread, autochthonous species in this river, often used in human nutrition. For assessment of DNA damage we used Comet assay (single cell gel electrophoresis) SCGE. The study was carried out on the site Visnjica, situated downstream Belgrade (Serbian capitol) which is identified as one of the major hotspots of faecal pollution along the Danube River. Basic physico-chemical and microbiological parameters of water quality were monitored along with the sampling of fish blood, liver and gills in February, April, August and November 2014. Comets were randomly scored and analyzed using Comet IV computer software (Perceptive Instruments, UK). DNA damage level is expressed using Tail Intensity (TI). All three tissues had highest level of DNA damage in August, which was month with the highest water temperature. Blood had the lowest DNA damage level in November, gills in April and liver in February. Similar response in all tissues was noticed with slight variations which is expected considering the differences in the level of exposure and metabolism in the selected tissues. Freshwater bream is shown to be a potentially good indicator organism in field studies of genotoxic potential.",
publisher = "US National Institute of Environmental Health Science, US Environmental Protection Agency",
journal = "The Central & Eastern Europe Conference on Health and the Environment (CEECHE), Prague, Czech Republic",
title = "Seasonal changes in genotoxic potential of the Danube river assessed by comet assay on tissues of freshwater bream",
pages = "151",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2760"
}

Kostić, J., Kolarević, S., Kracun-Kolarevic, M., Aborgiba, M., Gacic, Z., Lenhardt, M.,& Vuković-Gačić, B.. (2016). Seasonal changes in genotoxic potential of the Danube river assessed by comet assay on tissues of freshwater bream. in The Central & Eastern Europe Conference on Health and the Environment (CEECHE), Prague, Czech Republic
US National Institute of Environmental Health Science, US Environmental Protection Agency., 151.
https://hdl.handle.net/21.15107/rcub_rimsi_2760

Kostić J, Kolarević S, Kracun-Kolarevic M, Aborgiba M, Gacic Z, Lenhardt M, Vuković-Gačić B. Seasonal changes in genotoxic potential of the Danube river assessed by comet assay on tissues of freshwater bream. in The Central & Eastern Europe Conference on Health and the Environment (CEECHE), Prague, Czech Republic. 2016;:151.
https://hdl.handle.net/21.15107/rcub_rimsi_2760 .

Kostić, Jovana, Kolarević, Stoimir, Kracun-Kolarevic, Margareta, Aborgiba, Mustafa, Gacic, Zoran, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Seasonal changes in genotoxic potential of the Danube river assessed by comet assay on tissues of freshwater bream" in The Central & Eastern Europe Conference on Health and the Environment (CEECHE), Prague, Czech Republic (2016):151,
https://hdl.handle.net/21.15107/rcub_rimsi_2760 .

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarevic, Margareta
AU  - Kostić, Jovana
AU  - Aborgiba, Mustafa
AU  - Paunović, Momir
AU  - Simonovic, Predrag
AU  - Simić, Vladica
AU  - Milošković, Aleksandra
AU  - Gacic, Zoran
AU  - Vuković-Gačić, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2764
AB  - In this study we have performed a comprehensive genotoxicological survey along the 900 km of the Sava River - the major drainage basin of the Southeastern Europe and the greatest tributary to the Danube River with 945 km long and 97,713 km large catchment's area extending over Slovenia, Croatia, Bosnia and Herzegovina and Serbia. In total, 12 sites were chosen in compliance with routine monitoring program of the basin member states. The genotoxic potential was assessed by the complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak Alburnus alburnus /Albumoides bipunctatus). We wanted to investigate whether the variation in genotoxic potential along the river can be linked to hotspots of faecal and industrial pollution. Hotspots of faecal pollution were identified by bacterial indicators (Escherichia coli numbers) while hotspots related to industry were identified by assessment of concentration of metals in tissue of bleak. The results indicated presence of genotoxic potential along the river resulted from recent or prolonged exposure to genotoxic pressure which can be traced to deterioration of quality of Sava River by urban/industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).
PB  - US National Institute of Environmental Health Science, US Environmental Protection Agency
C3  - The Central & Eastern Europe Conference on Health and the Environment (CEECHE), Prague, Czech Republic
T1  - Evaluation of genotoxic pressure along the Sava River
SP  - 150
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2764
ER  - 

@conference{
author = "Kolarević, Stoimir and Kracun-Kolarevic, Margareta and Kostić, Jovana and Aborgiba, Mustafa and Paunović, Momir and Simonovic, Predrag and Simić, Vladica and Milošković, Aleksandra and Gacic, Zoran and Vuković-Gačić, Branka",
year = "2016",
abstract = "In this study we have performed a comprehensive genotoxicological survey along the 900 km of the Sava River - the major drainage basin of the Southeastern Europe and the greatest tributary to the Danube River with 945 km long and 97,713 km large catchment's area extending over Slovenia, Croatia, Bosnia and Herzegovina and Serbia. In total, 12 sites were chosen in compliance with routine monitoring program of the basin member states. The genotoxic potential was assessed by the complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak Alburnus alburnus /Albumoides bipunctatus). We wanted to investigate whether the variation in genotoxic potential along the river can be linked to hotspots of faecal and industrial pollution. Hotspots of faecal pollution were identified by bacterial indicators (Escherichia coli numbers) while hotspots related to industry were identified by assessment of concentration of metals in tissue of bleak. The results indicated presence of genotoxic potential along the river resulted from recent or prolonged exposure to genotoxic pressure which can be traced to deterioration of quality of Sava River by urban/industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).",
publisher = "US National Institute of Environmental Health Science, US Environmental Protection Agency",
journal = "The Central & Eastern Europe Conference on Health and the Environment (CEECHE), Prague, Czech Republic",
title = "Evaluation of genotoxic pressure along the Sava River",
pages = "150",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2764"
}

Kolarević, S., Kracun-Kolarevic, M., Kostić, J., Aborgiba, M., Paunović, M., Simonovic, P., Simić, V., Milošković, A., Gacic, Z.,& Vuković-Gačić, B.. (2016). Evaluation of genotoxic pressure along the Sava River. in The Central & Eastern Europe Conference on Health and the Environment (CEECHE), Prague, Czech Republic
US National Institute of Environmental Health Science, US Environmental Protection Agency., 150.
https://hdl.handle.net/21.15107/rcub_rimsi_2764

Kolarević S, Kracun-Kolarevic M, Kostić J, Aborgiba M, Paunović M, Simonovic P, Simić V, Milošković A, Gacic Z, Vuković-Gačić B. Evaluation of genotoxic pressure along the Sava River. in The Central & Eastern Europe Conference on Health and the Environment (CEECHE), Prague, Czech Republic. 2016;:150.
https://hdl.handle.net/21.15107/rcub_rimsi_2764 .

Kolarević, Stoimir, Kracun-Kolarevic, Margareta, Kostić, Jovana, Aborgiba, Mustafa, Paunović, Momir, Simonovic, Predrag, Simić, Vladica, Milošković, Aleksandra, Gacic, Zoran, Vuković-Gačić, Branka, "Evaluation of genotoxic pressure along the Sava River" in The Central & Eastern Europe Conference on Health and the Environment (CEECHE), Prague, Czech Republic (2016):150,
https://hdl.handle.net/21.15107/rcub_rimsi_2764 .

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarevic, Margareta
AU  - Kostić, Jovana
AU  - Gacic, Zoran
AU  - Aborgiba, Mustafa
AU  - Farnleitner, Andreas
AU  - Reischer, Georg
AU  - Linke, Rita
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2648
AB  - The contamination of water by faecal pollution leads to exposure to pathogens via drinking water
production, recreation or irrigation. However, monitoring of microbiological quality of surface waters
is quite neglected despite its importance for human health. In the case of Sava River Basin, many
of the settlements situated on the river banks discharge high quantities of untreated or improperly
treated wastewaters directly into surface waters. Due to usage of water for irrigation, the evaluation of microbiological quality of the Sava River becomes essential for further river management.
Water samples were collected during September 2014 on 17 sites and during September 2015 on
15 sites situated along the Sava River. In 2015, additional samples were collected from 4 wastewater outlets detected onsite. Microbiological analyses comprised monitoring the standard indicators of faecal pollution within the surveys and long term monitoring data (obtained within 5 years of routine monitoring at 4 stations). For detection of total coliforms, Escherichia coli and enterococci, Defined Substrate Technology (DST) was used with quantification performed by Colilert Quanti-Tray 2000 system, which provides a Most Probable Number result. Detection of presumptive Clostridium perfringens was performed by membrane filtration method according to ISO 14189:2013. To identify the origin of pollution, microbial source tracking (MST) analyses were employed based on the human-associated BacHum and HF183II, the ruminant-associated BacR and the pigassociated Pig2Bac genetic Bacteroidetes faecal markers. Microbiological indicators showed the existence of hotsposts of faecal pollution in the Sava River. MST confirmed that the pollution is human associated. Long term data at selected sites indicated persistent faecal contamination which leads to conclusion that the sites are under the impact of continuous discharge of wastewaters.
PB  - Macedonian Ecological Society
C3  - V Congress of Ecologists of the Republic of Macedonia with International Participation, Ohrid, Macedonia
T1  - Assessment of the faecal contamination along the Sava River and identification of pollution sources
SP  - 179
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2648
ER  - 

@conference{
author = "Kolarević, Stoimir and Kracun-Kolarevic, Margareta and Kostić, Jovana and Gacic, Zoran and Aborgiba, Mustafa and Farnleitner, Andreas and Reischer, Georg and Linke, Rita and Paunović, Momir and Vuković-Gačić, Branka",
year = "2016",
abstract = "The contamination of water by faecal pollution leads to exposure to pathogens via drinking water
production, recreation or irrigation. However, monitoring of microbiological quality of surface waters
is quite neglected despite its importance for human health. In the case of Sava River Basin, many
of the settlements situated on the river banks discharge high quantities of untreated or improperly
treated wastewaters directly into surface waters. Due to usage of water for irrigation, the evaluation of microbiological quality of the Sava River becomes essential for further river management.
Water samples were collected during September 2014 on 17 sites and during September 2015 on
15 sites situated along the Sava River. In 2015, additional samples were collected from 4 wastewater outlets detected onsite. Microbiological analyses comprised monitoring the standard indicators of faecal pollution within the surveys and long term monitoring data (obtained within 5 years of routine monitoring at 4 stations). For detection of total coliforms, Escherichia coli and enterococci, Defined Substrate Technology (DST) was used with quantification performed by Colilert Quanti-Tray 2000 system, which provides a Most Probable Number result. Detection of presumptive Clostridium perfringens was performed by membrane filtration method according to ISO 14189:2013. To identify the origin of pollution, microbial source tracking (MST) analyses were employed based on the human-associated BacHum and HF183II, the ruminant-associated BacR and the pigassociated Pig2Bac genetic Bacteroidetes faecal markers. Microbiological indicators showed the existence of hotsposts of faecal pollution in the Sava River. MST confirmed that the pollution is human associated. Long term data at selected sites indicated persistent faecal contamination which leads to conclusion that the sites are under the impact of continuous discharge of wastewaters.",
publisher = "Macedonian Ecological Society",
journal = "V Congress of Ecologists of the Republic of Macedonia with International Participation, Ohrid, Macedonia",
title = "Assessment of the faecal contamination along the Sava River and identification of pollution sources",
pages = "179",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2648"
}

Kolarević, S., Kracun-Kolarevic, M., Kostić, J., Gacic, Z., Aborgiba, M., Farnleitner, A., Reischer, G., Linke, R., Paunović, M.,& Vuković-Gačić, B.. (2016). Assessment of the faecal contamination along the Sava River and identification of pollution sources. in V Congress of Ecologists of the Republic of Macedonia with International Participation, Ohrid, Macedonia
Macedonian Ecological Society., 179.
https://hdl.handle.net/21.15107/rcub_rimsi_2648

Kolarević S, Kracun-Kolarevic M, Kostić J, Gacic Z, Aborgiba M, Farnleitner A, Reischer G, Linke R, Paunović M, Vuković-Gačić B. Assessment of the faecal contamination along the Sava River and identification of pollution sources. in V Congress of Ecologists of the Republic of Macedonia with International Participation, Ohrid, Macedonia. 2016;:179.
https://hdl.handle.net/21.15107/rcub_rimsi_2648 .

Kolarević, Stoimir, Kracun-Kolarevic, Margareta, Kostić, Jovana, Gacic, Zoran, Aborgiba, Mustafa, Farnleitner, Andreas, Reischer, Georg, Linke, Rita, Paunović, Momir, Vuković-Gačić, Branka, "Assessment of the faecal contamination along the Sava River and identification of pollution sources" in V Congress of Ecologists of the Republic of Macedonia with International Participation, Ohrid, Macedonia (2016):179,
https://hdl.handle.net/21.15107/rcub_rimsi_2648 .

TY  - CONF
AU  - Kostić, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarevic, Margareta
AU  - Aborgiba, Mustafa
AU  - Gacic, Zoran
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2646
AB  - Fish species living in freshwater ecosystems are exposed to a various range of xenobiotics.
Two of the most important pathways of xenobiotics intake by fish are either via the digestive or
respiratory routes or by absorption of waterborne chemicals through the gills. As a biomarker of
exposure to genotoxic agents, the single cell gel electrophoresis (SCGE), the comet assay, is widely used for detecting DNA damage in different tissues of aquatic organisms. As biomarkers of effect, histopathological analyses are considered efficient and sensitive for monitoring the fish health and pollution of aquatic ecosystems. Examination of multiple biomarkers in exposed organisms may give important information on organisms’ exposure to xenobiotics and related stress response. The main objective of this study was to evaluate the level of the Sava River pollution in the Belgrade region by measuring genotoxic and histopathological biomarker responses in liver and gills of cyprinid fish. Specimens of Abramis brama, Blicca bjoerkna and Ballerus sapa, were collected in winter, spring, summer and autumn of 2014 from the site Duboko. This site is exposed to untreated urban wastewaters, agricultural and industrial activity. DNA damage in liver and gills is expressed by Tail Intensity (TI), while histological changes were expressed as organ index which represents the extent of organ injuries. The highest level of DNA damage in liver and gills was detected in summer. The lowest level of DNA damage in liver was observed during spring and in gills during autumn. HP index of liver (IL) and gills (IG) showed no significant difference between seasons, but IL showed gradual increase from winter to autumn and IG showed a gradual increase from winter to summer. In conclusion, summer is marked as a season in which fish are under higher pressure of pollution according to both DNA damage and histopathological changes in tissues.
PB  - Macedonian Ecological Society
C3  - V Congress of Ecologists of the Republic of Macedonia with International Participation, Ohrid, Macedonia
T1  - DNA damage and histological changes in gills and liver of cyprinid fish as biomarkers of the Sava River pollution
SP  - 176
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2646
ER  - 

@conference{
author = "Kostić, Jovana and Kolarević, Stoimir and Kracun-Kolarevic, Margareta and Aborgiba, Mustafa and Gacic, Zoran and Raskovic, Bozidar and Poleksic, Vesna and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2016",
abstract = "Fish species living in freshwater ecosystems are exposed to a various range of xenobiotics.
Two of the most important pathways of xenobiotics intake by fish are either via the digestive or
respiratory routes or by absorption of waterborne chemicals through the gills. As a biomarker of
exposure to genotoxic agents, the single cell gel electrophoresis (SCGE), the comet assay, is widely used for detecting DNA damage in different tissues of aquatic organisms. As biomarkers of effect, histopathological analyses are considered efficient and sensitive for monitoring the fish health and pollution of aquatic ecosystems. Examination of multiple biomarkers in exposed organisms may give important information on organisms’ exposure to xenobiotics and related stress response. The main objective of this study was to evaluate the level of the Sava River pollution in the Belgrade region by measuring genotoxic and histopathological biomarker responses in liver and gills of cyprinid fish. Specimens of Abramis brama, Blicca bjoerkna and Ballerus sapa, were collected in winter, spring, summer and autumn of 2014 from the site Duboko. This site is exposed to untreated urban wastewaters, agricultural and industrial activity. DNA damage in liver and gills is expressed by Tail Intensity (TI), while histological changes were expressed as organ index which represents the extent of organ injuries. The highest level of DNA damage in liver and gills was detected in summer. The lowest level of DNA damage in liver was observed during spring and in gills during autumn. HP index of liver (IL) and gills (IG) showed no significant difference between seasons, but IL showed gradual increase from winter to autumn and IG showed a gradual increase from winter to summer. In conclusion, summer is marked as a season in which fish are under higher pressure of pollution according to both DNA damage and histopathological changes in tissues.",
publisher = "Macedonian Ecological Society",
journal = "V Congress of Ecologists of the Republic of Macedonia with International Participation, Ohrid, Macedonia",
title = "DNA damage and histological changes in gills and liver of cyprinid fish as biomarkers of the Sava River pollution",
pages = "176",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2646"
}

Kostić, J., Kolarević, S., Kracun-Kolarevic, M., Aborgiba, M., Gacic, Z., Raskovic, B., Poleksic, V., Lenhardt, M.,& Vuković-Gačić, B.. (2016). DNA damage and histological changes in gills and liver of cyprinid fish as biomarkers of the Sava River pollution. in V Congress of Ecologists of the Republic of Macedonia with International Participation, Ohrid, Macedonia
Macedonian Ecological Society., 176.
https://hdl.handle.net/21.15107/rcub_rimsi_2646

Kostić J, Kolarević S, Kracun-Kolarevic M, Aborgiba M, Gacic Z, Raskovic B, Poleksic V, Lenhardt M, Vuković-Gačić B. DNA damage and histological changes in gills and liver of cyprinid fish as biomarkers of the Sava River pollution. in V Congress of Ecologists of the Republic of Macedonia with International Participation, Ohrid, Macedonia. 2016;:176.
https://hdl.handle.net/21.15107/rcub_rimsi_2646 .

Kostić, Jovana, Kolarević, Stoimir, Kracun-Kolarevic, Margareta, Aborgiba, Mustafa, Gacic, Zoran, Raskovic, Bozidar, Poleksic, Vesna, Lenhardt, Mirjana, Vuković-Gačić, Branka, "DNA damage and histological changes in gills and liver of cyprinid fish as biomarkers of the Sava River pollution" in V Congress of Ecologists of the Republic of Macedonia with International Participation, Ohrid, Macedonia (2016):176,
https://hdl.handle.net/21.15107/rcub_rimsi_2646 .

TY  - CONF
AU  - Kostić, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarevic, Margareta
AU  - Aborgiba, Mustafa
AU  - Gacic, Zoran
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2651
AB  - Freshwater ecosystems are constantly deteriorated due to the multitude of human activities, intensification of the industrial processes, wastewater discharge and agricultural chemicals. Besides, climate change provokes extreme weather and hydrological conditions leading to prolonged periods of droughts and floods. Floods can cause movements of polutants already present in the environment and introduce pathogenic bacteria in the flooded area. Many of the substances that reach environment could exhibit genotoxic potential on the genetic material of aquatic organisms, either alone or in complex mixtures of chemicals. Unfavorable hydrological conditions during 2014 resulted in extensive flooding in May in the Sava River basin. Obrenovac city was one of the most affected settlements on the river with esstimation that nearly 90% of town area was flooded. This led to evacuation of majority of inhabitants from the affected area. Our research was conducted from January to December 2014 on the Sava River, site Duboko, situated
downstream of Obrenovac city. This is the area of intensive agricultural activity. Few km upstream the sampling site is the mouth of Kolubara river which is receiving untreated municipal wastewater of the town Obrenovac (50.000 inhabitants) and thermal power plant Nikola Tesla A with belonging ash fild. On monthly basis we have performed the measurements of basic phisico-chemical parameters, sampling water for microbiology analysis and fish tissue for the comet assay. Single Cell Gel Electrophoresis (SGCE) or Comet assay is widley accepted tool in ecogenotoxicology studies, in which DNA damage is measured on the level of a single eukaryotic cell. For the detection and quantification of E. coli and Enterococci we have used enzymathic methods and MPN approach. For comet assay analysis blood, liver and gill tissues were sampled from two cyprinid fishes. The highest water level was observed in May 2014, when flooding occured. Evacuation of inhabitants had led to exclusion of urban wastewater discharge which was refelcted through the lowest concentration of faecal pollution indicator bacteria, E. coli and Enterococci. With return of inhabitants in June their concentration in water started to increase gradually. In contrast, with withdrawl of water from the flooded agricultural land and ash disposal field high amounts of potentialy genotoxic substances were introduced into the Sava river, which was observed in sudden increase of DNA damage level in all three fish tissues during June. This study showed that flood occurence had major impact on the microbiological quality and genotoxic potential of the Sava River. Faecal pollution is mainly under the impact of urban wastewater discharge. On the other hand, genotoxic substances are most likely of industrial and agricultural origin.
Acknowledgements
This research was funded by project OI173045 „Fishes as water quality indicators in open waters of Serbia” (Ministry of Education, Science and Technological Development of the Republic of Serbia). This research was conducted in the Laboratory at the Chair of Microbiology, Center for genotoxicology and ecogenotoxicology, Faculty of Biology, University of Belgrade, and Institute for Multidisciplinary research, University of Belgrade.
PB  - European Communities 7th Framework Programme under Grant Agreement no 603629-ENV-2013-6.2.1-GLOBAQUA
C3  - 1st GLOBAQUA International Conference Managing The Effects Of Multiple Stressors On Aquatic Ecosystems Under Water Scarcity, Freising , Germany
T1  - Effect of floods on DNA damage of two cyprinid fish in the Sava River
SP  - 111
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2651
ER  - 

@conference{
author = "Kostić, Jovana and Kolarević, Stoimir and Kracun-Kolarevic, Margareta and Aborgiba, Mustafa and Gacic, Zoran and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2016",
abstract = "Freshwater ecosystems are constantly deteriorated due to the multitude of human activities, intensification of the industrial processes, wastewater discharge and agricultural chemicals. Besides, climate change provokes extreme weather and hydrological conditions leading to prolonged periods of droughts and floods. Floods can cause movements of polutants already present in the environment and introduce pathogenic bacteria in the flooded area. Many of the substances that reach environment could exhibit genotoxic potential on the genetic material of aquatic organisms, either alone or in complex mixtures of chemicals. Unfavorable hydrological conditions during 2014 resulted in extensive flooding in May in the Sava River basin. Obrenovac city was one of the most affected settlements on the river with esstimation that nearly 90% of town area was flooded. This led to evacuation of majority of inhabitants from the affected area. Our research was conducted from January to December 2014 on the Sava River, site Duboko, situated
downstream of Obrenovac city. This is the area of intensive agricultural activity. Few km upstream the sampling site is the mouth of Kolubara river which is receiving untreated municipal wastewater of the town Obrenovac (50.000 inhabitants) and thermal power plant Nikola Tesla A with belonging ash fild. On monthly basis we have performed the measurements of basic phisico-chemical parameters, sampling water for microbiology analysis and fish tissue for the comet assay. Single Cell Gel Electrophoresis (SGCE) or Comet assay is widley accepted tool in ecogenotoxicology studies, in which DNA damage is measured on the level of a single eukaryotic cell. For the detection and quantification of E. coli and Enterococci we have used enzymathic methods and MPN approach. For comet assay analysis blood, liver and gill tissues were sampled from two cyprinid fishes. The highest water level was observed in May 2014, when flooding occured. Evacuation of inhabitants had led to exclusion of urban wastewater discharge which was refelcted through the lowest concentration of faecal pollution indicator bacteria, E. coli and Enterococci. With return of inhabitants in June their concentration in water started to increase gradually. In contrast, with withdrawl of water from the flooded agricultural land and ash disposal field high amounts of potentialy genotoxic substances were introduced into the Sava river, which was observed in sudden increase of DNA damage level in all three fish tissues during June. This study showed that flood occurence had major impact on the microbiological quality and genotoxic potential of the Sava River. Faecal pollution is mainly under the impact of urban wastewater discharge. On the other hand, genotoxic substances are most likely of industrial and agricultural origin.
Acknowledgements
This research was funded by project OI173045 „Fishes as water quality indicators in open waters of Serbia” (Ministry of Education, Science and Technological Development of the Republic of Serbia). This research was conducted in the Laboratory at the Chair of Microbiology, Center for genotoxicology and ecogenotoxicology, Faculty of Biology, University of Belgrade, and Institute for Multidisciplinary research, University of Belgrade.",
publisher = "European Communities 7th Framework Programme under Grant Agreement no 603629-ENV-2013-6.2.1-GLOBAQUA",
journal = "1st GLOBAQUA International Conference Managing The Effects Of Multiple Stressors On Aquatic Ecosystems Under Water Scarcity, Freising , Germany",
title = "Effect of floods on DNA damage of two cyprinid fish in the Sava River",
pages = "111",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2651"
}

Kostić, J., Kolarević, S., Kracun-Kolarevic, M., Aborgiba, M., Gacic, Z., Lenhardt, M.,& Vuković-Gačić, B.. (2016). Effect of floods on DNA damage of two cyprinid fish in the Sava River. in 1st GLOBAQUA International Conference Managing The Effects Of Multiple Stressors On Aquatic Ecosystems Under Water Scarcity, Freising , Germany
European Communities 7th Framework Programme under Grant Agreement no 603629-ENV-2013-6.2.1-GLOBAQUA., 111.
https://hdl.handle.net/21.15107/rcub_rimsi_2651

Kostić J, Kolarević S, Kracun-Kolarevic M, Aborgiba M, Gacic Z, Lenhardt M, Vuković-Gačić B. Effect of floods on DNA damage of two cyprinid fish in the Sava River. in 1st GLOBAQUA International Conference Managing The Effects Of Multiple Stressors On Aquatic Ecosystems Under Water Scarcity, Freising , Germany. 2016;:111.
https://hdl.handle.net/21.15107/rcub_rimsi_2651 .

Kostić, Jovana, Kolarević, Stoimir, Kracun-Kolarevic, Margareta, Aborgiba, Mustafa, Gacic, Zoran, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Effect of floods on DNA damage of two cyprinid fish in the Sava River" in 1st GLOBAQUA International Conference Managing The Effects Of Multiple Stressors On Aquatic Ecosystems Under Water Scarcity, Freising , Germany (2016):111,
https://hdl.handle.net/21.15107/rcub_rimsi_2651 .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Aborgiba, Mustafa
AU  - Kracun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Simonović, Predrag
AU  - Simic, Vladica
AU  - Milosković, Aleksandra
AU  - Reischer, Georg
AU  - Farnleitner, Andreas
AU  - Gačić, Zoran
AU  - Milacic, Radmila
AU  - Zuliani, Tea
AU  - Vidmar, Janja
AU  - Pergal, Marija
AU  - Piria, Marina
AU  - Paunović, Momir
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1007
AB  - In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Evaluation of Genotoxic Pressure along the Sava River
IS  - 9
VL  - 11
DO  - 10.1371/journal.pone.0162450
ER  - 

@article{
author = "Kolarević, Stoimir and Aborgiba, Mustafa and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Simonović, Predrag and Simic, Vladica and Milosković, Aleksandra and Reischer, Georg and Farnleitner, Andreas and Gačić, Zoran and Milacic, Radmila and Zuliani, Tea and Vidmar, Janja and Pergal, Marija and Piria, Marina and Paunović, Momir and Vukovic-Gacic, Branka",
year = "2016",
abstract = "In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Evaluation of Genotoxic Pressure along the Sava River",
number = "9",
volume = "11",
doi = "10.1371/journal.pone.0162450"
}

Kolarević, S., Aborgiba, M., Kracun-Kolarević, M., Kostić-Vuković, J., Simonović, P., Simic, V., Milosković, A., Reischer, G., Farnleitner, A., Gačić, Z., Milacic, R., Zuliani, T., Vidmar, J., Pergal, M., Piria, M., Paunović, M.,& Vukovic-Gacic, B.. (2016). Evaluation of Genotoxic Pressure along the Sava River. in PLoS One
Public Library Science, San Francisco., 11(9).
https://doi.org/10.1371/journal.pone.0162450

Kolarević S, Aborgiba M, Kracun-Kolarević M, Kostić-Vuković J, Simonović P, Simic V, Milosković A, Reischer G, Farnleitner A, Gačić Z, Milacic R, Zuliani T, Vidmar J, Pergal M, Piria M, Paunović M, Vukovic-Gacic B. Evaluation of Genotoxic Pressure along the Sava River. in PLoS One. 2016;11(9).
doi:10.1371/journal.pone.0162450 .

Kolarević, Stoimir, Aborgiba, Mustafa, Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Simonović, Predrag, Simic, Vladica, Milosković, Aleksandra, Reischer, Georg, Farnleitner, Andreas, Gačić, Zoran, Milacic, Radmila, Zuliani, Tea, Vidmar, Janja, Pergal, Marija, Piria, Marina, Paunović, Momir, Vukovic-Gacic, Branka, "Evaluation of Genotoxic Pressure along the Sava River" in PLoS One, 11, no. 9 (2016),
https://doi.org/10.1371/journal.pone.0162450 . .

TY  - JOUR
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Aborgiba, Mustafa
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1023
AB  - This study examines the use of freshwater bream (Abramis brama) as a sentinel organism for genotoxicity assessment of the Danube River using the comet assay. Sampling of bream was performed during February, April, August, and November in 2014 to assess seasonal variation of DNA damage level as a response to genotoxicity in annual cycle. Additionally, concentrations of fecal coliforms and enterococci were analyzed and they indicated a critical to strong level of fecal pollution on investigated locality during annual cycle. Comet assay was performed on blood, liver, and gill cells of bream. DNA damage level was expressed using tail intensity (TI %), Olive tail moment (OTM), and tail length (TL pix). According to TI and OTM, all three tissues had the highest level of DNA damage in August. The lowest level of DNA damage in liver was measured during February, in blood during November, and in gills during April. According to TL, gills had the highest level of DNA damage in February, and liver cells had the lowest level of damage during April. Multiple correspondence analysis (MCA) showed that DNA damage in blood cells is under the strong influence of variations in NO2, NO3 (-), NH4 (+) levels and also the variation in temperature and oxygen levels. DNA damage in liver cells is highly associated with the variations of Mn, Fe, Cu, Zn, and PO4 (3-) levels. DNA damage in gill cells is strongly affected by the variations of As, Cd, Pb, Cr, and COD (Mn) levels. Freshwater bream is shown to be a potentially good indicator organism in genotoxic potential field studies.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Genotoxicity assessment of the Danube River using tissues of freshwater bream (Abramis brama)
EP  - 20795
IS  - 20
SP  - 20783
VL  - 23
DO  - 10.1007/s11356-016-7213-0
ER  - 

@article{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Aborgiba, Mustafa and Gačić, Zoran and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2016",
abstract = "This study examines the use of freshwater bream (Abramis brama) as a sentinel organism for genotoxicity assessment of the Danube River using the comet assay. Sampling of bream was performed during February, April, August, and November in 2014 to assess seasonal variation of DNA damage level as a response to genotoxicity in annual cycle. Additionally, concentrations of fecal coliforms and enterococci were analyzed and they indicated a critical to strong level of fecal pollution on investigated locality during annual cycle. Comet assay was performed on blood, liver, and gill cells of bream. DNA damage level was expressed using tail intensity (TI %), Olive tail moment (OTM), and tail length (TL pix). According to TI and OTM, all three tissues had the highest level of DNA damage in August. The lowest level of DNA damage in liver was measured during February, in blood during November, and in gills during April. According to TL, gills had the highest level of DNA damage in February, and liver cells had the lowest level of damage during April. Multiple correspondence analysis (MCA) showed that DNA damage in blood cells is under the strong influence of variations in NO2, NO3 (-), NH4 (+) levels and also the variation in temperature and oxygen levels. DNA damage in liver cells is highly associated with the variations of Mn, Fe, Cu, Zn, and PO4 (3-) levels. DNA damage in gill cells is strongly affected by the variations of As, Cd, Pb, Cr, and COD (Mn) levels. Freshwater bream is shown to be a potentially good indicator organism in genotoxic potential field studies.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Genotoxicity assessment of the Danube River using tissues of freshwater bream (Abramis brama)",
pages = "20795-20783",
number = "20",
volume = "23",
doi = "10.1007/s11356-016-7213-0"
}

Kostić-Vuković, J., Kolarević, S., Kracun-Kolarević, M., Aborgiba, M., Gačić, Z., Lenhardt, M.,& Vukovic-Gacic, B.. (2016). Genotoxicity assessment of the Danube River using tissues of freshwater bream (Abramis brama). in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 23(20), 20783-20795.
https://doi.org/10.1007/s11356-016-7213-0

Kostić-Vuković J, Kolarević S, Kracun-Kolarević M, Aborgiba M, Gačić Z, Lenhardt M, Vukovic-Gacic B. Genotoxicity assessment of the Danube River using tissues of freshwater bream (Abramis brama). in Environmental Science and Pollution Research. 2016;23(20):20783-20795.
doi:10.1007/s11356-016-7213-0 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Aborgiba, Mustafa, Gačić, Zoran, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "Genotoxicity assessment of the Danube River using tissues of freshwater bream (Abramis brama)" in Environmental Science and Pollution Research, 23, no. 20 (2016):20783-20795,
https://doi.org/10.1007/s11356-016-7213-0 . .

TY  - JOUR
AU  - Vrzel, Janja
AU  - Vukovic-Gacic, Branka
AU  - Kolarević, Stoimir
AU  - Gačić, Zoran
AU  - Kracun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Aborgiba, Mustafa
AU  - Farnleitner, Andreas
AU  - Reischer, Georg
AU  - Linke, Rita
AU  - Paunović, Momir
AU  - Ogrinc, Nives
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1016
AB  - Coupled measurements of nitrate (NO3-), nitrogen (N), and oxygen (O) isotopic composition (delta N-15(NO3) and delta O-18(NO3)) were used to investigate the sources and processes of N cycling, while the microbial source tracking (MST) method was used to identify microbiological pollution in the surface water of the Sava River Basin (SRB) in autumn in 2014 and 2015 during high and low water discharge. Atmospheric nitrate deposition or nitrate-containing fertilizers were found not to be significant sources of riverine nitrate in the SRB. The ranges of isotope values suggest that NO3- in the SRB derives from soil nitrification, sewage, and/or manure, which were further supported by MST analysis. Microbiological indicators show the existence of hotspots of fecal pollution in the SRB, which are human associated. Long-term observations indicate persistent fecal contamination at selected locations caused by continuous discharge of untreated wastewaters into the SRB.
PB  - Elsevier, Amsterdam
T2  - Science of the Total Environment
T1  - Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin
EP  - 1471
SP  - 1460
VL  - 573
DO  - 10.1016/j.scitotenv.2016.07.213
ER  - 

@article{
author = "Vrzel, Janja and Vukovic-Gacic, Branka and Kolarević, Stoimir and Gačić, Zoran and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Aborgiba, Mustafa and Farnleitner, Andreas and Reischer, Georg and Linke, Rita and Paunović, Momir and Ogrinc, Nives",
year = "2016",
abstract = "Coupled measurements of nitrate (NO3-), nitrogen (N), and oxygen (O) isotopic composition (delta N-15(NO3) and delta O-18(NO3)) were used to investigate the sources and processes of N cycling, while the microbial source tracking (MST) method was used to identify microbiological pollution in the surface water of the Sava River Basin (SRB) in autumn in 2014 and 2015 during high and low water discharge. Atmospheric nitrate deposition or nitrate-containing fertilizers were found not to be significant sources of riverine nitrate in the SRB. The ranges of isotope values suggest that NO3- in the SRB derives from soil nitrification, sewage, and/or manure, which were further supported by MST analysis. Microbiological indicators show the existence of hotspots of fecal pollution in the SRB, which are human associated. Long-term observations indicate persistent fecal contamination at selected locations caused by continuous discharge of untreated wastewaters into the SRB.",
publisher = "Elsevier, Amsterdam",
journal = "Science of the Total Environment",
title = "Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin",
pages = "1471-1460",
volume = "573",
doi = "10.1016/j.scitotenv.2016.07.213"
}

Vrzel, J., Vukovic-Gacic, B., Kolarević, S., Gačić, Z., Kracun-Kolarević, M., Kostić-Vuković, J., Aborgiba, M., Farnleitner, A., Reischer, G., Linke, R., Paunović, M.,& Ogrinc, N.. (2016). Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin. in Science of the Total Environment
Elsevier, Amsterdam., 573, 1460-1471.
https://doi.org/10.1016/j.scitotenv.2016.07.213

Vrzel J, Vukovic-Gacic B, Kolarević S, Gačić Z, Kracun-Kolarević M, Kostić-Vuković J, Aborgiba M, Farnleitner A, Reischer G, Linke R, Paunović M, Ogrinc N. Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin. in Science of the Total Environment. 2016;573:1460-1471.
doi:10.1016/j.scitotenv.2016.07.213 .

Vrzel, Janja, Vukovic-Gacic, Branka, Kolarević, Stoimir, Gačić, Zoran, Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Aborgiba, Mustafa, Farnleitner, Andreas, Reischer, Georg, Linke, Rita, Paunović, Momir, Ogrinc, Nives, "Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin" in Science of the Total Environment, 573 (2016):1460-1471,
https://doi.org/10.1016/j.scitotenv.2016.07.213 . .

TY  - JOUR
AU  - Aborgiba, Mustafa
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Elbahi, Samia
AU  - Knezevic-Vukcević, Jelena
AU  - Lenhardt, Mirjana
AU  - Paunović, Momir
AU  - Gačić, Zoran
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/936
AB  - Extreme hydrological events, such as water scarcity and flooding, can modify the effect of other stressors present in aquatic environment, which could result in the significant changes in the ecosystem functioning. Presence and interaction of various stressors (genotoxic pollutants) in the environment can influence the integrity of DNA molecules in aquatic organisms which can be negatively reflected on the individual, population and community levels. Therefore, in this study we have investigated the impact of flooding, in terms of genotoxicity, on organisms belonging to different trophic levels. The study was carried out on the site situated in the lower stretch of the Sava River which faced devastating effects of severe flooding in May 2014. The flooding occurred during our field experiment and this event provided a unique opportunity to assess its influence to the environment. The in situ effects of this specific situation were monitored by measuring physical, chemical and microbiological parameters of water, and by comparing the level of DNA damage in coelomocytes and haemocytes of freshwater worms Branchiura sowerbyi, haemocytes of freshwater mussels Unto tumidus and blood cells of freshwater fish Abramis bjoerkna/Abramis sapa, by means of the comet assay. Our study indicated that the flooding had a significant impact on water quality by decreasing the amount and discharge rate of urban wastewaters but simultaneously introducing contaminants from the nearby fly ash disposal field into river by runoff, which had diverse effects on the level of DNA damage in the studied organisms. This indicates that the assessment of genotoxic pollution in situ is strongly affected by the choice of the bioindicator organism.
PB  - Elsevier, Amsterdam
T2  - Science of the Total Environment
T1  - Flooding modifies the genotoxic effects of pollution on a worm, a mussel and two fish species from the Sava River
EP  - 367
SP  - 358
VL  - 540
DO  - 10.1016/j.scitotenv.2015.03.120
ER  - 

@article{
author = "Aborgiba, Mustafa and Kostić-Vuković, Jovana and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Elbahi, Samia and Knezevic-Vukcević, Jelena and Lenhardt, Mirjana and Paunović, Momir and Gačić, Zoran and Vukovic-Gacic, Branka",
year = "2016",
abstract = "Extreme hydrological events, such as water scarcity and flooding, can modify the effect of other stressors present in aquatic environment, which could result in the significant changes in the ecosystem functioning. Presence and interaction of various stressors (genotoxic pollutants) in the environment can influence the integrity of DNA molecules in aquatic organisms which can be negatively reflected on the individual, population and community levels. Therefore, in this study we have investigated the impact of flooding, in terms of genotoxicity, on organisms belonging to different trophic levels. The study was carried out on the site situated in the lower stretch of the Sava River which faced devastating effects of severe flooding in May 2014. The flooding occurred during our field experiment and this event provided a unique opportunity to assess its influence to the environment. The in situ effects of this specific situation were monitored by measuring physical, chemical and microbiological parameters of water, and by comparing the level of DNA damage in coelomocytes and haemocytes of freshwater worms Branchiura sowerbyi, haemocytes of freshwater mussels Unto tumidus and blood cells of freshwater fish Abramis bjoerkna/Abramis sapa, by means of the comet assay. Our study indicated that the flooding had a significant impact on water quality by decreasing the amount and discharge rate of urban wastewaters but simultaneously introducing contaminants from the nearby fly ash disposal field into river by runoff, which had diverse effects on the level of DNA damage in the studied organisms. This indicates that the assessment of genotoxic pollution in situ is strongly affected by the choice of the bioindicator organism.",
publisher = "Elsevier, Amsterdam",
journal = "Science of the Total Environment",
title = "Flooding modifies the genotoxic effects of pollution on a worm, a mussel and two fish species from the Sava River",
pages = "367-358",
volume = "540",
doi = "10.1016/j.scitotenv.2015.03.120"
}

Aborgiba, M., Kostić-Vuković, J., Kolarević, S., Kracun-Kolarević, M., Elbahi, S., Knezevic-Vukcević, J., Lenhardt, M., Paunović, M., Gačić, Z.,& Vukovic-Gacic, B.. (2016). Flooding modifies the genotoxic effects of pollution on a worm, a mussel and two fish species from the Sava River. in Science of the Total Environment
Elsevier, Amsterdam., 540, 358-367.
https://doi.org/10.1016/j.scitotenv.2015.03.120

Aborgiba M, Kostić-Vuković J, Kolarević S, Kracun-Kolarević M, Elbahi S, Knezevic-Vukcević J, Lenhardt M, Paunović M, Gačić Z, Vukovic-Gacic B. Flooding modifies the genotoxic effects of pollution on a worm, a mussel and two fish species from the Sava River. in Science of the Total Environment. 2016;540:358-367.
doi:10.1016/j.scitotenv.2015.03.120 .

Aborgiba, Mustafa, Kostić-Vuković, Jovana, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Elbahi, Samia, Knezevic-Vukcević, Jelena, Lenhardt, Mirjana, Paunović, Momir, Gačić, Zoran, Vukovic-Gacic, Branka, "Flooding modifies the genotoxic effects of pollution on a worm, a mussel and two fish species from the Sava River" in Science of the Total Environment, 540 (2016):358-367,
https://doi.org/10.1016/j.scitotenv.2015.03.120 . .

TY  - CONF
AU  - Kracun-Kolarevic, Margareta
AU  - Paunović, Momir
AU  - Kolarević, Stoimir
AU  - Aborgiba, Mustafa
AU  - Kostić, Jovana
AU  - Gacic, Zoran
AU  - Vuković-Gačić, Branka
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2876
PB  - National Institute of Biology and Jožef Stefan Institute, Slovenia
C3  - 22nd International Symposium on Environmental Biogeochemistry: Dynamics of Biogeochemical Systems: Processes and Modeling, Piran, Slovenia
T1  - Freshwater mussels and worms as bioindicators in the ecogenotoxicological studies
SP  - 62
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2876
ER  - 

@conference{
author = "Kracun-Kolarevic, Margareta and Paunović, Momir and Kolarević, Stoimir and Aborgiba, Mustafa and Kostić, Jovana and Gacic, Zoran and Vuković-Gačić, Branka",
year = "2015",
publisher = "National Institute of Biology and Jožef Stefan Institute, Slovenia",
journal = "22nd International Symposium on Environmental Biogeochemistry: Dynamics of Biogeochemical Systems: Processes and Modeling, Piran, Slovenia",
title = "Freshwater mussels and worms as bioindicators in the ecogenotoxicological studies",
pages = "62",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2876"
}

Kracun-Kolarevic, M., Paunović, M., Kolarević, S., Aborgiba, M., Kostić, J., Gacic, Z.,& Vuković-Gačić, B.. (2015). Freshwater mussels and worms as bioindicators in the ecogenotoxicological studies. in 22nd International Symposium on Environmental Biogeochemistry: Dynamics of Biogeochemical Systems: Processes and Modeling, Piran, Slovenia
National Institute of Biology and Jožef Stefan Institute, Slovenia., 62.
https://hdl.handle.net/21.15107/rcub_rimsi_2876

Kracun-Kolarevic M, Paunović M, Kolarević S, Aborgiba M, Kostić J, Gacic Z, Vuković-Gačić B. Freshwater mussels and worms as bioindicators in the ecogenotoxicological studies. in 22nd International Symposium on Environmental Biogeochemistry: Dynamics of Biogeochemical Systems: Processes and Modeling, Piran, Slovenia. 2015;:62.
https://hdl.handle.net/21.15107/rcub_rimsi_2876 .

Kracun-Kolarevic, Margareta, Paunović, Momir, Kolarević, Stoimir, Aborgiba, Mustafa, Kostić, Jovana, Gacic, Zoran, Vuković-Gačić, Branka, "Freshwater mussels and worms as bioindicators in the ecogenotoxicological studies" in 22nd International Symposium on Environmental Biogeochemistry: Dynamics of Biogeochemical Systems: Processes and Modeling, Piran, Slovenia (2015):62,
https://hdl.handle.net/21.15107/rcub_rimsi_2876 .

TY  - CONF
AU  - Kostić, Jovana
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarevic, Margareta
AU  - Aborgiba, Mustafa
AU  - Knežević-Vukčević, Jelena
AU  - Vuković-Gačić, Branka
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2630
AB  - UVOD: Mikrobiološki indikatori sanitarnog i organskog zagađenja čine neophodan segment svake procene kvaliteta vode. Međutim, kako se velike količine neprerađenih ili nepropisno prerađenih otpadnih voda ispuštaju u vodotokove teško je razdvojiti sanitarno od organskog zagađenja. Vizuelni prikaz rezultata IBR metodom pokazao se veoma korisnim u analizi međusobnih odnosa različitih biomarkera i ima široku primenu u biomonitoringu. Osim primene na biomarkerima, metoda može da pokaže i uzajamne odnose različitih hemijskih parametara. Metoda IBR do sada nije korišćena za analizu uzajamnih odnosa mikrobioloških parametara.
CILJEVI: Primena IBR metode u proceni kvaliteta vode, pri čemu su mikrobiološki parametri fekalnog i organskog zagađenja korišćeni kao “biomarkeri“.
METODE: Za potrebe ove studije odabrana su dva seta podataka. Prvi set je dobijen tokom višemesečnog monitoringa na reci Savi tokom 2014. godine, uključujući i period sa poplavom, tj. maj mesec. Od indikatora fekalnog zagađenja praćeni su totalni koliformi, Escherichia coli, Enterococcus faecalis i Clostridium perfringens. Nivo organskog zagađenja određen je na osnovu odnosa heterotrofnih i oligotrofnih bakterija, i indeksa fosfatazne aktivnosti. Drugi set podataka dobijen je tokom višemesečnog monitoringa sezonskog variranja mikrobioloških indikatora na reci Peštan, tokom 2012. godine. Od indikatora fekalnog zagađenja praćeni su totalni koliformi, E. coli i E. faecalis. Nivo organskog zagađenja određen je na osnovu odnosa heterotrofnih i oligotrofnih bakterija.
REZULTATI: Vizuelni prikaz rezultata IBR metodom dao je jasan uvid u minimalne i maksimalne vrednosti svakog od indikatora fekalnog i organskog zagađenja tokom različitih meseci. Za prvi set podataka, na osnovu grafika i na osnovu dobijenih IBR vrednosti, jasno se izdvaja mesec u kome je došlo do poplava. Za drugi set podataka očigledno je variranje mikrobioloških parametara tokom različitih sezona.
ZAKLJUČAK: Metoda IBR je pogodna za prikaz i analizu kompleksnog seta mikrobioloških podataka.
PB  - Udruženje mikrobiologa Srbije
C3  - X Konges Mikrobiologa Srbije "Mikromed 2015", Beograd, Srbija
T1  - Primena IBR metode (Integrated Biomarker Response) u mikrobiološkoj analizi vodenih ekosistema
EP  - 229
SP  - 228
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2630
ER  - 

@conference{
author = "Kostić, Jovana and Sunjog, Karolina and Kolarević, Stoimir and Kracun-Kolarevic, Margareta and Aborgiba, Mustafa and Knežević-Vukčević, Jelena and Vuković-Gačić, Branka",
year = "2015",
abstract = "UVOD: Mikrobiološki indikatori sanitarnog i organskog zagađenja čine neophodan segment svake procene kvaliteta vode. Međutim, kako se velike količine neprerađenih ili nepropisno prerađenih otpadnih voda ispuštaju u vodotokove teško je razdvojiti sanitarno od organskog zagađenja. Vizuelni prikaz rezultata IBR metodom pokazao se veoma korisnim u analizi međusobnih odnosa različitih biomarkera i ima široku primenu u biomonitoringu. Osim primene na biomarkerima, metoda može da pokaže i uzajamne odnose različitih hemijskih parametara. Metoda IBR do sada nije korišćena za analizu uzajamnih odnosa mikrobioloških parametara.
CILJEVI: Primena IBR metode u proceni kvaliteta vode, pri čemu su mikrobiološki parametri fekalnog i organskog zagađenja korišćeni kao “biomarkeri“.
METODE: Za potrebe ove studije odabrana su dva seta podataka. Prvi set je dobijen tokom višemesečnog monitoringa na reci Savi tokom 2014. godine, uključujući i period sa poplavom, tj. maj mesec. Od indikatora fekalnog zagađenja praćeni su totalni koliformi, Escherichia coli, Enterococcus faecalis i Clostridium perfringens. Nivo organskog zagađenja određen je na osnovu odnosa heterotrofnih i oligotrofnih bakterija, i indeksa fosfatazne aktivnosti. Drugi set podataka dobijen je tokom višemesečnog monitoringa sezonskog variranja mikrobioloških indikatora na reci Peštan, tokom 2012. godine. Od indikatora fekalnog zagađenja praćeni su totalni koliformi, E. coli i E. faecalis. Nivo organskog zagađenja određen je na osnovu odnosa heterotrofnih i oligotrofnih bakterija.
REZULTATI: Vizuelni prikaz rezultata IBR metodom dao je jasan uvid u minimalne i maksimalne vrednosti svakog od indikatora fekalnog i organskog zagađenja tokom različitih meseci. Za prvi set podataka, na osnovu grafika i na osnovu dobijenih IBR vrednosti, jasno se izdvaja mesec u kome je došlo do poplava. Za drugi set podataka očigledno je variranje mikrobioloških parametara tokom različitih sezona.
ZAKLJUČAK: Metoda IBR je pogodna za prikaz i analizu kompleksnog seta mikrobioloških podataka.",
publisher = "Udruženje mikrobiologa Srbije",
journal = "X Konges Mikrobiologa Srbije "Mikromed 2015", Beograd, Srbija",
title = "Primena IBR metode (Integrated Biomarker Response) u mikrobiološkoj analizi vodenih ekosistema",
pages = "229-228",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2630"
}

Kostić, J., Sunjog, K., Kolarević, S., Kracun-Kolarevic, M., Aborgiba, M., Knežević-Vukčević, J.,& Vuković-Gačić, B.. (2015). Primena IBR metode (Integrated Biomarker Response) u mikrobiološkoj analizi vodenih ekosistema. in X Konges Mikrobiologa Srbije "Mikromed 2015", Beograd, Srbija
Udruženje mikrobiologa Srbije., 228-229.
https://hdl.handle.net/21.15107/rcub_rimsi_2630

Kostić J, Sunjog K, Kolarević S, Kracun-Kolarevic M, Aborgiba M, Knežević-Vukčević J, Vuković-Gačić B. Primena IBR metode (Integrated Biomarker Response) u mikrobiološkoj analizi vodenih ekosistema. in X Konges Mikrobiologa Srbije "Mikromed 2015", Beograd, Srbija. 2015;:228-229.
https://hdl.handle.net/21.15107/rcub_rimsi_2630 .

Kostić, Jovana, Sunjog, Karolina, Kolarević, Stoimir, Kracun-Kolarevic, Margareta, Aborgiba, Mustafa, Knežević-Vukčević, Jelena, Vuković-Gačić, Branka, "Primena IBR metode (Integrated Biomarker Response) u mikrobiološkoj analizi vodenih ekosistema" in X Konges Mikrobiologa Srbije "Mikromed 2015", Beograd, Srbija (2015):228-229,
https://hdl.handle.net/21.15107/rcub_rimsi_2630 .

TY  - CONF
AU  - Kostić, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarevic, Margareta
AU  - Aborgiba, Mustafa
AU  - Knežević-Vukčević, Jelena
AU  - Lenhardt, Mirjana
AU  - Gacic, Zoran
AU  - Vuković-Gačić, Branka
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2628
AB  - U ovom istraživanju praćen je mikrobiološki kvalitet vode reke Save, na lokalitetu nizvodno od grada Obrenovca, pre i nakon velikih poplava koje su se dogodile u maju 2014. godine. Praćeni su indikatori fekalnog zagađenja (ukupni koliformi, Escherichia coli, Enterococcus faecalis) kao i pokazatelji organskog opterećenja (heterotrofi). Dobijeni rezultati pokazuju da je tokom meseca sa najvišim vodostajem (maj) došlo do velikog pada u koncentraciji glavnih pokazatelja fekalnog zagađenja, u prvom redu E. coli. Sa porastom vodostaja tokom perioda poplava uočen je i pad koncentracije heterotrofnih bakterija. S druge strane, detektovan je porast koncentracije ukupnih koliforma tokom poplava. U ovom ispitivanju jasno je pokazano da poplave dovode do promena u trendu variranja koncentracija mikrobioloških parametara kvaliteta vode.
PB  - Srpsko društvo za zaštitu voda
C3  - 44. Konferencija o aktuelnim problemima korišćenja i zaštite voda ’’Voda 2015“, Kopaonik, Srbija
T1  - Uticaj poplava na mikrobiološki kvalitet vode reke Save na teritoriji grada Obrenovca
EP  - 40
SP  - 35
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2628
ER  - 

@conference{
author = "Kostić, Jovana and Kolarević, Stoimir and Kracun-Kolarevic, Margareta and Aborgiba, Mustafa and Knežević-Vukčević, Jelena and Lenhardt, Mirjana and Gacic, Zoran and Vuković-Gačić, Branka",
year = "2015",
abstract = "U ovom istraživanju praćen je mikrobiološki kvalitet vode reke Save, na lokalitetu nizvodno od grada Obrenovca, pre i nakon velikih poplava koje su se dogodile u maju 2014. godine. Praćeni su indikatori fekalnog zagađenja (ukupni koliformi, Escherichia coli, Enterococcus faecalis) kao i pokazatelji organskog opterećenja (heterotrofi). Dobijeni rezultati pokazuju da je tokom meseca sa najvišim vodostajem (maj) došlo do velikog pada u koncentraciji glavnih pokazatelja fekalnog zagađenja, u prvom redu E. coli. Sa porastom vodostaja tokom perioda poplava uočen je i pad koncentracije heterotrofnih bakterija. S druge strane, detektovan je porast koncentracije ukupnih koliforma tokom poplava. U ovom ispitivanju jasno je pokazano da poplave dovode do promena u trendu variranja koncentracija mikrobioloških parametara kvaliteta vode.",
publisher = "Srpsko društvo za zaštitu voda",
journal = "44. Konferencija o aktuelnim problemima korišćenja i zaštite voda ’’Voda 2015“, Kopaonik, Srbija",
title = "Uticaj poplava na mikrobiološki kvalitet vode reke Save na teritoriji grada Obrenovca",
pages = "40-35",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2628"
}

Kostić, J., Kolarević, S., Kracun-Kolarevic, M., Aborgiba, M., Knežević-Vukčević, J., Lenhardt, M., Gacic, Z.,& Vuković-Gačić, B.. (2015). Uticaj poplava na mikrobiološki kvalitet vode reke Save na teritoriji grada Obrenovca. in 44. Konferencija o aktuelnim problemima korišćenja i zaštite voda ’’Voda 2015“, Kopaonik, Srbija
Srpsko društvo za zaštitu voda., 35-40.
https://hdl.handle.net/21.15107/rcub_rimsi_2628

Kostić J, Kolarević S, Kracun-Kolarevic M, Aborgiba M, Knežević-Vukčević J, Lenhardt M, Gacic Z, Vuković-Gačić B. Uticaj poplava na mikrobiološki kvalitet vode reke Save na teritoriji grada Obrenovca. in 44. Konferencija o aktuelnim problemima korišćenja i zaštite voda ’’Voda 2015“, Kopaonik, Srbija. 2015;:35-40.
https://hdl.handle.net/21.15107/rcub_rimsi_2628 .

Kostić, Jovana, Kolarević, Stoimir, Kracun-Kolarevic, Margareta, Aborgiba, Mustafa, Knežević-Vukčević, Jelena, Lenhardt, Mirjana, Gacic, Zoran, Vuković-Gačić, Branka, "Uticaj poplava na mikrobiološki kvalitet vode reke Save na teritoriji grada Obrenovca" in 44. Konferencija o aktuelnim problemima korišćenja i zaštite voda ’’Voda 2015“, Kopaonik, Srbija (2015):35-40,
https://hdl.handle.net/21.15107/rcub_rimsi_2628 .

TY  - CONF
AU  - Kostić, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarevic, Margareta
AU  - Aborgiba, Mustafa
AU  - Gacic, Zoran
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2652
AB  - A number of biological consequences of DNA damage can be initiated at the cellular, organ, whole animal, population and community levels. The comet assay, single cell gel electrophoresis (SCGE), is a sensitive and rapid technique for detection of DNA damage in individual cells based on the migration of denatured DNA during electrophoresis, in which damaged nuclei form comet-like shapes. Since, the large rivers are constantly exposed to variety of chemicals we tested genotoxic potential of rivers Sava and Danube in the Belgrade region, on cyprinid fish. DNA damage was monitored in blood, liver and gill cells of Blicca bjoerkna, Ballerus sapa and Abramis brama by comet assay. The specimens of B. bjoerkna and B. sapa were sampled at the site Duboko (Sava river), which is exposed to untreated municipal wastewater, effluents of the Thermal Power Plant and intensive agricultural activity. The specimens of A. brama were sampled at the site Višnjica (Danube river), which is exposed to untreated municipal wastewater from surrounding Belgrade municipalities. Sampling was performed during February and August 2014. Comet IV Computer Software (Perceptive Instruments, UK) was used for the quantification of DNA damage. The increased level of DNA damage obtained in all tissues of B. bjoerkna and B. sapa (Sava river), and A. brama (Danube river) is in correlation with high chemical pollution detected at Duboko and Višnjica sites. Moreover, this study showed the influence of seasonal variations on the level of DNA damage.
PB  - Природно-математички факултет, Бања Лука
C3  - III Simpozijum biologa i ekologa Republike Srpske
T1  - Evaluation of the Sava and the Danube river genotoxicity by Comet assay on different cyprinid fish
SP  - 203
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2652
ER  - 

@conference{
author = "Kostić, Jovana and Kolarević, Stoimir and Kracun-Kolarevic, Margareta and Aborgiba, Mustafa and Gacic, Zoran and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2015",
abstract = "A number of biological consequences of DNA damage can be initiated at the cellular, organ, whole animal, population and community levels. The comet assay, single cell gel electrophoresis (SCGE), is a sensitive and rapid technique for detection of DNA damage in individual cells based on the migration of denatured DNA during electrophoresis, in which damaged nuclei form comet-like shapes. Since, the large rivers are constantly exposed to variety of chemicals we tested genotoxic potential of rivers Sava and Danube in the Belgrade region, on cyprinid fish. DNA damage was monitored in blood, liver and gill cells of Blicca bjoerkna, Ballerus sapa and Abramis brama by comet assay. The specimens of B. bjoerkna and B. sapa were sampled at the site Duboko (Sava river), which is exposed to untreated municipal wastewater, effluents of the Thermal Power Plant and intensive agricultural activity. The specimens of A. brama were sampled at the site Višnjica (Danube river), which is exposed to untreated municipal wastewater from surrounding Belgrade municipalities. Sampling was performed during February and August 2014. Comet IV Computer Software (Perceptive Instruments, UK) was used for the quantification of DNA damage. The increased level of DNA damage obtained in all tissues of B. bjoerkna and B. sapa (Sava river), and A. brama (Danube river) is in correlation with high chemical pollution detected at Duboko and Višnjica sites. Moreover, this study showed the influence of seasonal variations on the level of DNA damage.",
publisher = "Природно-математички факултет, Бања Лука",
journal = "III Simpozijum biologa i ekologa Republike Srpske",
title = "Evaluation of the Sava and the Danube river genotoxicity by Comet assay on different cyprinid fish",
pages = "203",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2652"
}

Kostić, J., Kolarević, S., Kracun-Kolarevic, M., Aborgiba, M., Gacic, Z., Lenhardt, M.,& Vuković-Gačić, B.. (2015). Evaluation of the Sava and the Danube river genotoxicity by Comet assay on different cyprinid fish. in III Simpozijum biologa i ekologa Republike Srpske
Природно-математички факултет, Бања Лука., 203.
https://hdl.handle.net/21.15107/rcub_rimsi_2652

Kostić J, Kolarević S, Kracun-Kolarevic M, Aborgiba M, Gacic Z, Lenhardt M, Vuković-Gačić B. Evaluation of the Sava and the Danube river genotoxicity by Comet assay on different cyprinid fish. in III Simpozijum biologa i ekologa Republike Srpske. 2015;:203.
https://hdl.handle.net/21.15107/rcub_rimsi_2652 .

Kostić, Jovana, Kolarević, Stoimir, Kracun-Kolarevic, Margareta, Aborgiba, Mustafa, Gacic, Zoran, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Evaluation of the Sava and the Danube river genotoxicity by Comet assay on different cyprinid fish" in III Simpozijum biologa i ekologa Republike Srpske (2015):203,
https://hdl.handle.net/21.15107/rcub_rimsi_2652 .

TY  - CONF
AU  - Kostić, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarevic, Margareta
AU  - Aborgiba, Mustafa
AU  - Knežević-Vukčević, Jelena
AU  - Gacic, Zoran
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2580
AB  - Object of the research: During catastrophic floods in May 2014, in the Serbian part of the Sava River, Obrenovac city was the most affected with estimation that about 90% of populated area was flooded. This had led to a complete evacuation of inhabitants, giving us an opportunity to examine effects of municipal wastewater exclusion on the microbiological quality of the Sava River. To assess the genotoxic potential of the Sava River comet assay was applied on blood cells of two cyprinid fishes.

Materials and methods: Sampling was performed from January to December 2014, on the Sava River, site Duboko. Sanitary pollution was assessed by detection of total coliforms, E. coli and faecal enterococci using enzymatic methods. Organic pollution was estimated by isolation of heterotrophic bacteria on the YEA. Comet assay was performed on blood cells of white bream (Blicca bjoerkna) and white-eye bream (Ballerus sapa).

Results: The lowest levels of sanitary and organic pollution were recorded during May and June when inhabitants were evacuated, indicating the role of the municipal wastewater in the pollution. The level of DNA damage in blood cells highly correlated with heavy metals in water which originate from the ash disposal field.

Conclusions: Flooding have the potential to modify water quality by remobilization of pollutants already present in the environment which is reflected on the microbiological water quality and genotoxic potential measured by DNA damage.
PB  - Hellenic Microbiology Society
C3  - 9th Balkan Congress of Microbiology, Thessaloniki, Greece
T1  - The effects of flooding on microbiological quality and genotoxic potential of the Sava River
SP  - 166
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2580
ER  - 

@conference{
author = "Kostić, Jovana and Kolarević, Stoimir and Kracun-Kolarevic, Margareta and Aborgiba, Mustafa and Knežević-Vukčević, Jelena and Gacic, Zoran and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2015",
abstract = "Object of the research: During catastrophic floods in May 2014, in the Serbian part of the Sava River, Obrenovac city was the most affected with estimation that about 90% of populated area was flooded. This had led to a complete evacuation of inhabitants, giving us an opportunity to examine effects of municipal wastewater exclusion on the microbiological quality of the Sava River. To assess the genotoxic potential of the Sava River comet assay was applied on blood cells of two cyprinid fishes.

Materials and methods: Sampling was performed from January to December 2014, on the Sava River, site Duboko. Sanitary pollution was assessed by detection of total coliforms, E. coli and faecal enterococci using enzymatic methods. Organic pollution was estimated by isolation of heterotrophic bacteria on the YEA. Comet assay was performed on blood cells of white bream (Blicca bjoerkna) and white-eye bream (Ballerus sapa).

Results: The lowest levels of sanitary and organic pollution were recorded during May and June when inhabitants were evacuated, indicating the role of the municipal wastewater in the pollution. The level of DNA damage in blood cells highly correlated with heavy metals in water which originate from the ash disposal field.

Conclusions: Flooding have the potential to modify water quality by remobilization of pollutants already present in the environment which is reflected on the microbiological water quality and genotoxic potential measured by DNA damage.",
publisher = "Hellenic Microbiology Society",
journal = "9th Balkan Congress of Microbiology, Thessaloniki, Greece",
title = "The effects of flooding on microbiological quality and genotoxic potential of the Sava River",
pages = "166",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2580"
}

Kostić, J., Kolarević, S., Kracun-Kolarevic, M., Aborgiba, M., Knežević-Vukčević, J., Gacic, Z., Lenhardt, M.,& Vuković-Gačić, B.. (2015). The effects of flooding on microbiological quality and genotoxic potential of the Sava River. in 9th Balkan Congress of Microbiology, Thessaloniki, Greece
Hellenic Microbiology Society., 166.
https://hdl.handle.net/21.15107/rcub_rimsi_2580

Kostić J, Kolarević S, Kracun-Kolarevic M, Aborgiba M, Knežević-Vukčević J, Gacic Z, Lenhardt M, Vuković-Gačić B. The effects of flooding on microbiological quality and genotoxic potential of the Sava River. in 9th Balkan Congress of Microbiology, Thessaloniki, Greece. 2015;:166.
https://hdl.handle.net/21.15107/rcub_rimsi_2580 .

Kostić, Jovana, Kolarević, Stoimir, Kracun-Kolarevic, Margareta, Aborgiba, Mustafa, Knežević-Vukčević, Jelena, Gacic, Zoran, Lenhardt, Mirjana, Vuković-Gačić, Branka, "The effects of flooding on microbiological quality and genotoxic potential of the Sava River" in 9th Balkan Congress of Microbiology, Thessaloniki, Greece (2015):166,
https://hdl.handle.net/21.15107/rcub_rimsi_2580 .

TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Aborgiba, Mustafa
AU  - Knežević-Vukčević, Jelena
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1926
AB  - U ovom radu ispitivan je potencijal dve vrste roda Abramis (Abramis bjoerkna i Abramis
sapa) kao bioindikatora genotoksičnog potencijala vode reke Save primenom komet
testa. Izabrani lokalitet pripada oblasti intezivne poljoprivredne aktivnosti, izložen je otpadnim
vodama grada Obrenovca i nalazi se u blizini najveće termoelektrane u Srbiji, “Nikola
Tesla”. Uzorkovanje je vršeno od januara do avgusta 2014. godine, obuhvatajući mesec
maj kada je došlo do intezivnih poplava u slivu reke Save. Dobijeni rezultati ukazuju da se
ispitivane vrste mogu koristiti za procenu genotoksičnog potencijala.
PB  - University of Belgrade, Faculty of Agriculture
C3  - VII International Conference Water & Fish, Belgrade, Serbia
T1  - How flooding modifies genotoxic response in freshwater fish?
SP  - 333
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1926
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Aborgiba, Mustafa and Knežević-Vukčević, Jelena and Gačić, Zoran and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2015",
abstract = "U ovom radu ispitivan je potencijal dve vrste roda Abramis (Abramis bjoerkna i Abramis
sapa) kao bioindikatora genotoksičnog potencijala vode reke Save primenom komet
testa. Izabrani lokalitet pripada oblasti intezivne poljoprivredne aktivnosti, izložen je otpadnim
vodama grada Obrenovca i nalazi se u blizini najveće termoelektrane u Srbiji, “Nikola
Tesla”. Uzorkovanje je vršeno od januara do avgusta 2014. godine, obuhvatajući mesec
maj kada je došlo do intezivnih poplava u slivu reke Save. Dobijeni rezultati ukazuju da se
ispitivane vrste mogu koristiti za procenu genotoksičnog potencijala.",
publisher = "University of Belgrade, Faculty of Agriculture",
journal = "VII International Conference Water & Fish, Belgrade, Serbia",
title = "How flooding modifies genotoxic response in freshwater fish?",
pages = "333",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1926"
}

Kostić-Vuković, J., Kolarević, S., Kračun-Kolarević, M., Aborgiba, M., Knežević-Vukčević, J., Gačić, Z., Lenhardt, M.,& Vuković-Gačić, B.. (2015). How flooding modifies genotoxic response in freshwater fish?. in VII International Conference Water & Fish, Belgrade, Serbia
University of Belgrade, Faculty of Agriculture., 333.
https://hdl.handle.net/21.15107/rcub_rimsi_1926

Kostić-Vuković J, Kolarević S, Kračun-Kolarević M, Aborgiba M, Knežević-Vukčević J, Gačić Z, Lenhardt M, Vuković-Gačić B. How flooding modifies genotoxic response in freshwater fish?. in VII International Conference Water & Fish, Belgrade, Serbia. 2015;:333.
https://hdl.handle.net/21.15107/rcub_rimsi_1926 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Aborgiba, Mustafa, Knežević-Vukčević, Jelena, Gačić, Zoran, Lenhardt, Mirjana, Vuković-Gačić, Branka, "How flooding modifies genotoxic response in freshwater fish?" in VII International Conference Water & Fish, Belgrade, Serbia (2015):333,
https://hdl.handle.net/21.15107/rcub_rimsi_1926 .

TY  - CONF
AU  - Kostić, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarevic, Margareta
AU  - Aborgiba, Mustafa
AU  - Elbahi, Samia
AU  - Knežević-Vukčević, Jelena
AU  - Lenhardt, Mirjana
AU  - Gacic, Zoran
AU  - Vuković-Gačić, Branka
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2322
AB  - Cilj ovog istraživanja je procena kvaliteta vode reke Save u regionu Beograda, analizom nivoa oštećenja DNK  u ćelijama jetre, škrga i krvi kod krupatice. Uzorci su prikupljeni na lokalitetu Duboko, tokom januara, februara i marta 2014. godine. Za procenu genotoksičnog potencijala primenjen je komet test, a Olive tail moment (OTM) izabran je kao parametar za procenu oštećenja DNK. Za krv i jetru najviši nivo oštećenja zabeležen je u januaru, a za škrge u februaru. Smanjen nivo oštećenja za sva tri tkiva zabeležen je u martu. Preliminarni rezultati ukazuju da je krupatica pogodan test organizam za praćenje genotoksičnog potencijala.
PB  - Srpsko društvo za zaštitu voda
C3  - 43. Konferencija o aktuelnim problemima korišćenja i zaštite voda ’’Voda 2014“
T1  - Procena genotoksičnosti reke Save primenom komet testa na ribi krupatica (Abramis bjoerkna L. 1758)
SP  - 129
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2322
ER  - 

@conference{
author = "Kostić, Jovana and Kolarević, Stoimir and Kracun-Kolarevic, Margareta and Aborgiba, Mustafa and Elbahi, Samia and Knežević-Vukčević, Jelena and Lenhardt, Mirjana and Gacic, Zoran and Vuković-Gačić, Branka",
year = "2014",
abstract = "Cilj ovog istraživanja je procena kvaliteta vode reke Save u regionu Beograda, analizom nivoa oštećenja DNK  u ćelijama jetre, škrga i krvi kod krupatice. Uzorci su prikupljeni na lokalitetu Duboko, tokom januara, februara i marta 2014. godine. Za procenu genotoksičnog potencijala primenjen je komet test, a Olive tail moment (OTM) izabran je kao parametar za procenu oštećenja DNK. Za krv i jetru najviši nivo oštećenja zabeležen je u januaru, a za škrge u februaru. Smanjen nivo oštećenja za sva tri tkiva zabeležen je u martu. Preliminarni rezultati ukazuju da je krupatica pogodan test organizam za praćenje genotoksičnog potencijala.",
publisher = "Srpsko društvo za zaštitu voda",
journal = "43. Konferencija o aktuelnim problemima korišćenja i zaštite voda ’’Voda 2014“",
title = "Procena genotoksičnosti reke Save primenom komet testa na ribi krupatica (Abramis bjoerkna L. 1758)",
pages = "129",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2322"
}

Kostić, J., Kolarević, S., Kracun-Kolarevic, M., Aborgiba, M., Elbahi, S., Knežević-Vukčević, J., Lenhardt, M., Gacic, Z.,& Vuković-Gačić, B.. (2014). Procena genotoksičnosti reke Save primenom komet testa na ribi krupatica (Abramis bjoerkna L. 1758). in 43. Konferencija o aktuelnim problemima korišćenja i zaštite voda ’’Voda 2014“
Srpsko društvo za zaštitu voda., 129.
https://hdl.handle.net/21.15107/rcub_rimsi_2322

Kostić J, Kolarević S, Kracun-Kolarevic M, Aborgiba M, Elbahi S, Knežević-Vukčević J, Lenhardt M, Gacic Z, Vuković-Gačić B. Procena genotoksičnosti reke Save primenom komet testa na ribi krupatica (Abramis bjoerkna L. 1758). in 43. Konferencija o aktuelnim problemima korišćenja i zaštite voda ’’Voda 2014“. 2014;:129.
https://hdl.handle.net/21.15107/rcub_rimsi_2322 .

Kostić, Jovana, Kolarević, Stoimir, Kracun-Kolarevic, Margareta, Aborgiba, Mustafa, Elbahi, Samia, Knežević-Vukčević, Jelena, Lenhardt, Mirjana, Gacic, Zoran, Vuković-Gačić, Branka, "Procena genotoksičnosti reke Save primenom komet testa na ribi krupatica (Abramis bjoerkna L. 1758)" in 43. Konferencija o aktuelnim problemima korišćenja i zaštite voda ’’Voda 2014“ (2014):129,
https://hdl.handle.net/21.15107/rcub_rimsi_2322 .

TY  - CONF
AU  - Aborgiba, Mustafa
AU  - Elbahi, Samia
AU  - Kolarević, Stoimir
AU  - Kostić, Jovana
AU  - Kracun-Kolarevic, Margareta
AU  - Knežević-Vukčević, Jelena
AU  - Lenhardt, Mirjana
AU  - Gacic, Zoran
AU  - Vuković-Gačić, Branka
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2493
AB  - The aim of this study was to assess the genotoxic potential of Danube River at the site Višnjica (downstream Belgrade) on monthly basis over year 2014 in tissues of common bream (A. brama). The level of DNA damage in the three tissues, blood, gills and liver was measured by comet assay as one of the major tools for assessing pollution related genotoxicity in aquatic organisms. Images of scored nucleoids were analyzed using software (Comet Assay IV). Significant differences in the level of DNA damage were observed in different tissues. For all three tissues, levels of DNA damage were higher comparing to data obtained in our previous research on Cyprinidae family from unpolluted site Special Nature Reserve “Uvac”. These results indicate the presence of genotoxic pollution at the site Višnjica. The samples of tissues were also collected for histopatology and heavy metals analyses which will provide further insight in the origin and fate of the detected genotoxic influence. Physical and chemical parameters and microbiological quality of water assessed each month are in correlation with comet assay results.
PB  - Serbian Genetic Society, Belgrade, Serbia
C3  - V Congress of the Serbian Genetic Society
T1  - Assesment of genotoxic pollution of the Danube River with comet assay in different tissues of common bream (Abramis brama L. 1758)
SP  - 131
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2493
ER  - 

@conference{
author = "Aborgiba, Mustafa and Elbahi, Samia and Kolarević, Stoimir and Kostić, Jovana and Kracun-Kolarevic, Margareta and Knežević-Vukčević, Jelena and Lenhardt, Mirjana and Gacic, Zoran and Vuković-Gačić, Branka",
year = "2014",
abstract = "The aim of this study was to assess the genotoxic potential of Danube River at the site Višnjica (downstream Belgrade) on monthly basis over year 2014 in tissues of common bream (A. brama). The level of DNA damage in the three tissues, blood, gills and liver was measured by comet assay as one of the major tools for assessing pollution related genotoxicity in aquatic organisms. Images of scored nucleoids were analyzed using software (Comet Assay IV). Significant differences in the level of DNA damage were observed in different tissues. For all three tissues, levels of DNA damage were higher comparing to data obtained in our previous research on Cyprinidae family from unpolluted site Special Nature Reserve “Uvac”. These results indicate the presence of genotoxic pollution at the site Višnjica. The samples of tissues were also collected for histopatology and heavy metals analyses which will provide further insight in the origin and fate of the detected genotoxic influence. Physical and chemical parameters and microbiological quality of water assessed each month are in correlation with comet assay results.",
publisher = "Serbian Genetic Society, Belgrade, Serbia",
journal = "V Congress of the Serbian Genetic Society",
title = "Assesment of genotoxic pollution of the Danube River with comet assay in different tissues of common bream (Abramis brama L. 1758)",
pages = "131",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2493"
}

Aborgiba, M., Elbahi, S., Kolarević, S., Kostić, J., Kracun-Kolarevic, M., Knežević-Vukčević, J., Lenhardt, M., Gacic, Z.,& Vuković-Gačić, B.. (2014). Assesment of genotoxic pollution of the Danube River with comet assay in different tissues of common bream (Abramis brama L. 1758). in V Congress of the Serbian Genetic Society
Serbian Genetic Society, Belgrade, Serbia., 131.
https://hdl.handle.net/21.15107/rcub_rimsi_2493

Aborgiba M, Elbahi S, Kolarević S, Kostić J, Kracun-Kolarevic M, Knežević-Vukčević J, Lenhardt M, Gacic Z, Vuković-Gačić B. Assesment of genotoxic pollution of the Danube River with comet assay in different tissues of common bream (Abramis brama L. 1758). in V Congress of the Serbian Genetic Society. 2014;:131.
https://hdl.handle.net/21.15107/rcub_rimsi_2493 .

Aborgiba, Mustafa, Elbahi, Samia, Kolarević, Stoimir, Kostić, Jovana, Kracun-Kolarevic, Margareta, Knežević-Vukčević, Jelena, Lenhardt, Mirjana, Gacic, Zoran, Vuković-Gačić, Branka, "Assesment of genotoxic pollution of the Danube River with comet assay in different tissues of common bream (Abramis brama L. 1758)" in V Congress of the Serbian Genetic Society (2014):131,
https://hdl.handle.net/21.15107/rcub_rimsi_2493 .

TY  - CONF
AU  - Kostić, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarevic, Margareta
AU  - Aborgiba, Mustafa
AU  - Elbahi, Samia
AU  - Knežević-Vukčević, Jelena
AU  - Lenhardt, Mirjana
AU  - Gacic, Zoran
AU  - Vuković-Gačić, Branka
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2482
AB  - The aim of this study was to evaluate genotoxic potential of the Sava River during 2014, at site Duboko in the Belgrade region, which is under the impact of agricultural, urban and industrial activities. DNA damage was determined by alkaline comet assay in different tissues of silver bream (A.bjoerkna L. 1758). To obtain basic level of DNA damage, some specimens were subjected to acclimation for 20 days. The level of DNA damage in tissues of silver bream after acclimation correlated with the damage detected in populations of species from Cyprinidae family from unpolluted control site in Special Nature Reserve “Uvac”. Higher level of DNA damage in tissues of silver bream from the site Duboko comparing to data obtained after acclimation indicated the presence of genotoxic pollution. Physical and chemical parameters and microbiological quality of water were in correlation with comet assay. Tissue samples were also collected for histopathology and heavy metals analyses which will provide further insight in the origin and fate of detected genotoxic influence.
PB  - Serbian Genetic Society, Belgrade, Serbia
C3  - V Congress of the Serbian Genetic Society
T1  - Genotoxicity evaluation of the Sava River using comet assay on silver bream (Abramis bjoerkna L. 1758)
SP  - 142
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2482
ER  - 

@conference{
author = "Kostić, Jovana and Kolarević, Stoimir and Kracun-Kolarevic, Margareta and Aborgiba, Mustafa and Elbahi, Samia and Knežević-Vukčević, Jelena and Lenhardt, Mirjana and Gacic, Zoran and Vuković-Gačić, Branka",
year = "2014",
abstract = "The aim of this study was to evaluate genotoxic potential of the Sava River during 2014, at site Duboko in the Belgrade region, which is under the impact of agricultural, urban and industrial activities. DNA damage was determined by alkaline comet assay in different tissues of silver bream (A.bjoerkna L. 1758). To obtain basic level of DNA damage, some specimens were subjected to acclimation for 20 days. The level of DNA damage in tissues of silver bream after acclimation correlated with the damage detected in populations of species from Cyprinidae family from unpolluted control site in Special Nature Reserve “Uvac”. Higher level of DNA damage in tissues of silver bream from the site Duboko comparing to data obtained after acclimation indicated the presence of genotoxic pollution. Physical and chemical parameters and microbiological quality of water were in correlation with comet assay. Tissue samples were also collected for histopathology and heavy metals analyses which will provide further insight in the origin and fate of detected genotoxic influence.",
publisher = "Serbian Genetic Society, Belgrade, Serbia",
journal = "V Congress of the Serbian Genetic Society",
title = "Genotoxicity evaluation of the Sava River using comet assay on silver bream (Abramis bjoerkna L. 1758)",
pages = "142",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2482"
}

Kostić, J., Kolarević, S., Kracun-Kolarevic, M., Aborgiba, M., Elbahi, S., Knežević-Vukčević, J., Lenhardt, M., Gacic, Z.,& Vuković-Gačić, B.. (2014). Genotoxicity evaluation of the Sava River using comet assay on silver bream (Abramis bjoerkna L. 1758). in V Congress of the Serbian Genetic Society
Serbian Genetic Society, Belgrade, Serbia., 142.
https://hdl.handle.net/21.15107/rcub_rimsi_2482

Kostić J, Kolarević S, Kracun-Kolarevic M, Aborgiba M, Elbahi S, Knežević-Vukčević J, Lenhardt M, Gacic Z, Vuković-Gačić B. Genotoxicity evaluation of the Sava River using comet assay on silver bream (Abramis bjoerkna L. 1758). in V Congress of the Serbian Genetic Society. 2014;:142.
https://hdl.handle.net/21.15107/rcub_rimsi_2482 .

Kostić, Jovana, Kolarević, Stoimir, Kracun-Kolarevic, Margareta, Aborgiba, Mustafa, Elbahi, Samia, Knežević-Vukčević, Jelena, Lenhardt, Mirjana, Gacic, Zoran, Vuković-Gačić, Branka, "Genotoxicity evaluation of the Sava River using comet assay on silver bream (Abramis bjoerkna L. 1758)" in V Congress of the Serbian Genetic Society (2014):142,
https://hdl.handle.net/21.15107/rcub_rimsi_2482 .

TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Aborgiba, Mustafa
AU  - Elbahi, Samia
AU  - Knežević-Vukčević, Jelena
AU  - Lenhardt, Mirjana
AU  - Gacic, Zoran
AU  - Vuković-Gačić, Branka
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1732
AB  - Uvod
Akvatični ekosistemi obično su izloženi mešavini toksičnih supstanci. Farmaceutski proizvodi su predominantno sintetička jedinjenja i zahtevaju hemijsku stabilnost, kako bi delovali u neizmenjenoj formi. Usled visoke stabilnosti i otpornosti na degradaciju, većina ovih jedinjenja prolazi gotovo neizmenjena kroz tretman otpadnih voda i dugo se zadržava u prirodi. Akutna i hronična toksičnost takvih jedinjenja i njihov uticaj na genetički materijal vodenih organizama mogu dati podatke neophodne za naučno zasnovanu procenu rizika. Genotoksikologija proučava efekte zagađenja na jedinkama i populacijama, a niz bioloških posledica oštećenja na molekulu DNK može se manifestovati na nivou ćelija, organa i čitavog organizma, a i na nivou zajednica i populacija. Ribe predstavljaju najviši trofički stupanj u vodnim ekosistemima, a takođe se koriste u ljudskoj ishrani. U ispitivanjima genotoksičnosti različitih vodenih ekosistema ovi organizmi se široko koriste jer efikasno metabolišu i akumuliraju različite agense (potencijalne zagađivače) što se može ispoljiti na fiziološkom, histološkom i molekularnom nivou.
Materijal i metode
Komet test, test elektroforeze pojedinačnih ćelija (SCGE), je osetljiva i brza metoda za detekciju oštećenja DNK u pojedinačnim ćelijama. Zasnovana je na migraciji denaturisane DNK, tokom
elektroforeze, pri čemu oštećena jedra izgledaju kao komete. Izvođenje komet testa zasniva se na koracima lize, denaturacije, elektroforeze i neutralizacije. Rastvor za liziranje omogućava razaranje ćelijskih membrana i oslobađanje sadržaja; denaturacija omogućava rasplitanje dvolančane DNK, a u toku elektroforeze negativno naelektrisani DNK fragmenti kreću se ka pozitivnoj elektrodi, a ćelije se pod mikroskopom uočavaju kao komete - jedro (glava komete) i DNK fragmenti (rep komete). Posmatranje kometa vrši se pomoću fluorescentnog mikroskopa, a merenje kometa paketom Comet IV softver (Perceptive Instruments, UK). Procenat DNK u repu komete direktno korelira sa nivoom oštećenja, odnosno količinom jednolančanih i dvolančanih prekida u DNK molekulu. Kod riba se oštećenje molekula DNK može pratiti na ćelijama različitih tkiva, uključujući ćelije krvi, jetre i škrga. Ćelije ova tri tkiva imaju različite uloge u organizmu, pa se može očekivati različit odgovor na prisustvo genotoksičnih materija u životnoj sredini. Paralelno sa praćenjem nivoa oštećenja molekula DNK, neophodna je i procena kvaliteta voda na osnovu fizičko-hemijskih
parametara i mikrobiološkog kvaliteta (sanitarni i ekološki aspekt).
Rezultati
U našim prethodnim istraživanjima na školjkama iz familije Unionidae i ribama iz familija Cyprinidae i Salmonidae, za više rečnih tokova u Srbiji, korišćenjem komet testa, pokazan je genotoksični potencijal (Kolarević i sar., 2013; Vuković-Gačić i sar., 2013; Sunjog i
sar., 2014). Takođe, pokazana je direktna veza između zagađenja vodene sredine i nivoa oštećenja DNK molekula.
Zaključak
Na osnovu naših rezultata može se zaključiti da su školjke i ribe pogodni model organizmi za biomonitoring vodenih sredina i da se komet testom može detektovati genotoksični potencijal različitih polutanata.
PB  - Fakultet veterinarske medicine Univerziteta u Beogradu
C3  - Šesnaesto regionalno savetovanje iz kliničke patologije i terapije životinja, Clinica Veterinaria 2014
T1  - Procena genotoksičnog potencijala rečne vode primenom komet testa na različitim vrstama riba
EP  - 166
SP  - 164
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1732
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Aborgiba, Mustafa and Elbahi, Samia and Knežević-Vukčević, Jelena and Lenhardt, Mirjana and Gacic, Zoran and Vuković-Gačić, Branka",
year = "2014",
abstract = "Uvod
Akvatični ekosistemi obično su izloženi mešavini toksičnih supstanci. Farmaceutski proizvodi su predominantno sintetička jedinjenja i zahtevaju hemijsku stabilnost, kako bi delovali u neizmenjenoj formi. Usled visoke stabilnosti i otpornosti na degradaciju, većina ovih jedinjenja prolazi gotovo neizmenjena kroz tretman otpadnih voda i dugo se zadržava u prirodi. Akutna i hronična toksičnost takvih jedinjenja i njihov uticaj na genetički materijal vodenih organizama mogu dati podatke neophodne za naučno zasnovanu procenu rizika. Genotoksikologija proučava efekte zagađenja na jedinkama i populacijama, a niz bioloških posledica oštećenja na molekulu DNK može se manifestovati na nivou ćelija, organa i čitavog organizma, a i na nivou zajednica i populacija. Ribe predstavljaju najviši trofički stupanj u vodnim ekosistemima, a takođe se koriste u ljudskoj ishrani. U ispitivanjima genotoksičnosti različitih vodenih ekosistema ovi organizmi se široko koriste jer efikasno metabolišu i akumuliraju različite agense (potencijalne zagađivače) što se može ispoljiti na fiziološkom, histološkom i molekularnom nivou.
Materijal i metode
Komet test, test elektroforeze pojedinačnih ćelija (SCGE), je osetljiva i brza metoda za detekciju oštećenja DNK u pojedinačnim ćelijama. Zasnovana je na migraciji denaturisane DNK, tokom
elektroforeze, pri čemu oštećena jedra izgledaju kao komete. Izvođenje komet testa zasniva se na koracima lize, denaturacije, elektroforeze i neutralizacije. Rastvor za liziranje omogućava razaranje ćelijskih membrana i oslobađanje sadržaja; denaturacija omogućava rasplitanje dvolančane DNK, a u toku elektroforeze negativno naelektrisani DNK fragmenti kreću se ka pozitivnoj elektrodi, a ćelije se pod mikroskopom uočavaju kao komete - jedro (glava komete) i DNK fragmenti (rep komete). Posmatranje kometa vrši se pomoću fluorescentnog mikroskopa, a merenje kometa paketom Comet IV softver (Perceptive Instruments, UK). Procenat DNK u repu komete direktno korelira sa nivoom oštećenja, odnosno količinom jednolančanih i dvolančanih prekida u DNK molekulu. Kod riba se oštećenje molekula DNK može pratiti na ćelijama različitih tkiva, uključujući ćelije krvi, jetre i škrga. Ćelije ova tri tkiva imaju različite uloge u organizmu, pa se može očekivati različit odgovor na prisustvo genotoksičnih materija u životnoj sredini. Paralelno sa praćenjem nivoa oštećenja molekula DNK, neophodna je i procena kvaliteta voda na osnovu fizičko-hemijskih
parametara i mikrobiološkog kvaliteta (sanitarni i ekološki aspekt).
Rezultati
U našim prethodnim istraživanjima na školjkama iz familije Unionidae i ribama iz familija Cyprinidae i Salmonidae, za više rečnih tokova u Srbiji, korišćenjem komet testa, pokazan je genotoksični potencijal (Kolarević i sar., 2013; Vuković-Gačić i sar., 2013; Sunjog i
sar., 2014). Takođe, pokazana je direktna veza između zagađenja vodene sredine i nivoa oštećenja DNK molekula.
Zaključak
Na osnovu naših rezultata može se zaključiti da su školjke i ribe pogodni model organizmi za biomonitoring vodenih sredina i da se komet testom može detektovati genotoksični potencijal različitih polutanata.",
publisher = "Fakultet veterinarske medicine Univerziteta u Beogradu",
journal = "Šesnaesto regionalno savetovanje iz kliničke patologije i terapije životinja, Clinica Veterinaria 2014",
title = "Procena genotoksičnog potencijala rečne vode primenom komet testa na različitim vrstama riba",
pages = "166-164",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1732"
}

Kostić-Vuković, J., Kolarević, S., Kračun-Kolarević, M., Aborgiba, M., Elbahi, S., Knežević-Vukčević, J., Lenhardt, M., Gacic, Z.,& Vuković-Gačić, B.. (2014). Procena genotoksičnog potencijala rečne vode primenom komet testa na različitim vrstama riba. in Šesnaesto regionalno savetovanje iz kliničke patologije i terapije životinja, Clinica Veterinaria 2014
Fakultet veterinarske medicine Univerziteta u Beogradu., 164-166.
https://hdl.handle.net/21.15107/rcub_rimsi_1732

Kostić-Vuković J, Kolarević S, Kračun-Kolarević M, Aborgiba M, Elbahi S, Knežević-Vukčević J, Lenhardt M, Gacic Z, Vuković-Gačić B. Procena genotoksičnog potencijala rečne vode primenom komet testa na različitim vrstama riba. in Šesnaesto regionalno savetovanje iz kliničke patologije i terapije životinja, Clinica Veterinaria 2014. 2014;:164-166.
https://hdl.handle.net/21.15107/rcub_rimsi_1732 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Aborgiba, Mustafa, Elbahi, Samia, Knežević-Vukčević, Jelena, Lenhardt, Mirjana, Gacic, Zoran, Vuković-Gačić, Branka, "Procena genotoksičnog potencijala rečne vode primenom komet testa na različitim vrstama riba" in Šesnaesto regionalno savetovanje iz kliničke patologije i terapije životinja, Clinica Veterinaria 2014 (2014):164-166,
https://hdl.handle.net/21.15107/rcub_rimsi_1732 .