TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Iličić, Renata
AU  - Milovanović, Predrag
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1919
AB  - The aim of this work was to identify and characterize the pectolytic bacteria responsible
for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo
F1) grown in the Futog locality (Baˇcka, Vojvodina), known for the five-century-long tradition of
cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were
observed during August 2021, while the inner tissues were macerated, featuring cream to black
discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence
ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP)
medium were characterized for their phenotypic and genotypic features. The pathogenicity was
confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase
chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as
well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus
sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were
identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as
Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating
the presence of diverse Pectobacterium species even in combined infection in the same field. Among
the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and
P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus
sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA,
dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the
phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was
found to be the most virulent species for cabbage, followed by P. versatile, while all three species
had comparable virulence with respect to potato. The results obtained in this work provide a better
understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.
PB  - MDPI
T2  - Microorganisms
T1  - Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia
IS  - 2
SP  - 335
VL  - 11
DO  - 10.3390/microorganisms11020335
ER  - 

@article{
author = "Jelušić, Aleksandra and Mitrović, Petar and Marković, Sanja and Iličić, Renata and Milovanović, Predrag and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this work was to identify and characterize the pectolytic bacteria responsible
for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo
F1) grown in the Futog locality (Baˇcka, Vojvodina), known for the five-century-long tradition of
cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were
observed during August 2021, while the inner tissues were macerated, featuring cream to black
discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence
ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP)
medium were characterized for their phenotypic and genotypic features. The pathogenicity was
confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase
chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as
well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus
sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were
identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as
Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating
the presence of diverse Pectobacterium species even in combined infection in the same field. Among
the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and
P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus
sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA,
dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the
phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was
found to be the most virulent species for cabbage, followed by P. versatile, while all three species
had comparable virulence with respect to potato. The results obtained in this work provide a better
understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia",
number = "2",
pages = "335",
volume = "11",
doi = "10.3390/microorganisms11020335"
}

Jelušić, A., Mitrović, P., Marković, S., Iličić, R., Milovanović, P., Stanković, S.,& Popović Milovanović, T.. (2023). Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia. in Microorganisms
MDPI., 11(2), 335.
https://doi.org/10.3390/microorganisms11020335

Jelušić A, Mitrović P, Marković S, Iličić R, Milovanović P, Stanković S, Popović Milovanović T. Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia. in Microorganisms. 2023;11(2):335.
doi:10.3390/microorganisms11020335 .

Jelušić, Aleksandra, Mitrović, Petar, Marković, Sanja, Iličić, Renata, Milovanović, Predrag, Stanković, Slaviša, Popović Milovanović, Tatjana, "Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia" in Microorganisms, 11, no. 2 (2023):335,
https://doi.org/10.3390/microorganisms11020335 . .

TY  - CONF
AU  - Marković, Sanja
AU  - Popović, Tatjana
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1959
AB  - In August 2021, symptoms of soft rot appeared on cabbage 
in Futog (Bačka, Vojvodina) locality known for traditional 
cabbage cultivation in Serbia. Symptoms appeared in the 
form of sunken and soft lesions on outer head leaves, while 
interior tissue was macerated, with cream to black 
discoloration. A strong, specific odor followed the 
breakdown of the affected tissue. From the 1 ha field, a 
total of 5 heads were collected for the isolation of causing 
pathogen. Margins of healthy and dead tissues were 
macerated and plated on Crystal Violet Pectate medium. 
Five creamy-white colonies forming pits in the medium 
were purified. These bacterial isolates were positive for 
pectinolytic activity on cabbage heads and potato slices. 
They were all facultative anaerobic, Gram-negative, 
catalase-positive, and oxidase-negative; unable to produce 
diffusible fluorescent pigments. Multilocus sequence 
typing of cabbage isolates was performed by sequencing 
of four housekeeping genes (proA, dnaX, icdA, mdh). 
Based on the nucleotide BLAST (National Center for 
Biotechnology Information, NCBI) analysis of the 
sequences of genes icdA and mdh, Serbian cabbage isolates 
were 100% identical with Pectobacterium carotovorum
subsp. odoriferum strain BC S7. Based on the dnaX gene, 
identity with the same strain was 99.79% and 99.57% 
based on the proA gene. Serbian cabbage isolates also 
showed 100% identity with other NCBI deposited P. 
odoriferum strains CFBP1878 and ATCC 25272 based on 
the icdA gene, as well as ICMP 11533 based on mdh gene. 
Therefore, this first report of P. odoriferum indicates a 
wider range of Pectobacterium spp. recently described in 
Serbia.
PB  - Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria  Progetto grafico - editoriale esecutivo, Diletta Pini
C3  - 14th International Conference   on Plant Pathogenic Bacteria
T1  - First report of Pectobacterium odoriferum causing  bacterial soft rot of cabbage in Serbia
SP  - 123
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1959
ER  - 

@conference{
author = "Marković, Sanja and Popović, Tatjana and Mitrović, Petar and Iličić, Renata and Jelušić, Aleksandra",
year = "2022",
abstract = "In August 2021, symptoms of soft rot appeared on cabbage 
in Futog (Bačka, Vojvodina) locality known for traditional 
cabbage cultivation in Serbia. Symptoms appeared in the 
form of sunken and soft lesions on outer head leaves, while 
interior tissue was macerated, with cream to black 
discoloration. A strong, specific odor followed the 
breakdown of the affected tissue. From the 1 ha field, a 
total of 5 heads were collected for the isolation of causing 
pathogen. Margins of healthy and dead tissues were 
macerated and plated on Crystal Violet Pectate medium. 
Five creamy-white colonies forming pits in the medium 
were purified. These bacterial isolates were positive for 
pectinolytic activity on cabbage heads and potato slices. 
They were all facultative anaerobic, Gram-negative, 
catalase-positive, and oxidase-negative; unable to produce 
diffusible fluorescent pigments. Multilocus sequence 
typing of cabbage isolates was performed by sequencing 
of four housekeeping genes (proA, dnaX, icdA, mdh). 
Based on the nucleotide BLAST (National Center for 
Biotechnology Information, NCBI) analysis of the 
sequences of genes icdA and mdh, Serbian cabbage isolates 
were 100% identical with Pectobacterium carotovorum
subsp. odoriferum strain BC S7. Based on the dnaX gene, 
identity with the same strain was 99.79% and 99.57% 
based on the proA gene. Serbian cabbage isolates also 
showed 100% identity with other NCBI deposited P. 
odoriferum strains CFBP1878 and ATCC 25272 based on 
the icdA gene, as well as ICMP 11533 based on mdh gene. 
Therefore, this first report of P. odoriferum indicates a 
wider range of Pectobacterium spp. recently described in 
Serbia.",
publisher = "Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria  Progetto grafico - editoriale esecutivo, Diletta Pini",
journal = "14th International Conference   on Plant Pathogenic Bacteria",
title = "First report of Pectobacterium odoriferum causing  bacterial soft rot of cabbage in Serbia",
pages = "123",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1959"
}

Marković, S., Popović, T., Mitrović, P., Iličić, R.,& Jelušić, A.. (2022). First report of Pectobacterium odoriferum causing  bacterial soft rot of cabbage in Serbia. in 14th International Conference   on Plant Pathogenic Bacteria
Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria  Progetto grafico - editoriale esecutivo, Diletta Pini., 123.
https://hdl.handle.net/21.15107/rcub_rimsi_1959

Marković S, Popović T, Mitrović P, Iličić R, Jelušić A. First report of Pectobacterium odoriferum causing  bacterial soft rot of cabbage in Serbia. in 14th International Conference   on Plant Pathogenic Bacteria. 2022;:123.
https://hdl.handle.net/21.15107/rcub_rimsi_1959 .

Marković, Sanja, Popović, Tatjana, Mitrović, Petar, Iličić, Renata, Jelušić, Aleksandra, "First report of Pectobacterium odoriferum causing  bacterial soft rot of cabbage in Serbia" in 14th International Conference   on Plant Pathogenic Bacteria (2022):123,
https://hdl.handle.net/21.15107/rcub_rimsi_1959 .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Berić, Tanja
AU  - Dimkić, Ivica
AU  - Mitrović, Petar
AU  - Stanković, Slaviša
AU  - Marković, Sanja
AU  - Popović, Tatjana
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1950
AB  - Background:
Yield and quality of oilseed rape and other Brassicacceae vegetables are affected with plant pathogenic bacterium 
Xanthomonas campestris pv. campestris (Xcc), both worldwide and in Serbia. Considering the losses that can arise as
a consequence of the infection with this pathogen, there is an increasing need for finding the effective and environ mentally safe method for its suppression.
Objectives:
With the aim to find a promising biological control agent for managing Xcc infection on winter oilseed rape in Serbia, 
this research was based on evaluation of the in vitro antagonistic potential of the Bacillus- and Pseudomonas-like
isolates obtained from the phyllosphere and rhizosphere of the diseased and healthy winter oilseed rape plants.
Methods:
Well diffusion technique was used to evaluate the potential inhibitory activity of the whole cultures and cell-free 
supernatants of the 36 Bacillus- and Pseudomonas-like isolates, against the collection of Serbian winter oilseed rape 
Xcc isolates. DNA of the isolates which were able to inhibit Xcc growth was amplified with the primers based on the 
sequences of tuf and gyrB genes for Bacillus- and Pseudomonas-like isolates, respectively and sequenced.
Results:
Nine Bacillus spp. isolates whose whole cultures showed antagonistic potential were identified as B. thuringiensis/B. 
cereus (5); B. valezensis (1); B. mobilis (1); B. megaterium (1) and B. halotolerans (1), while six Pseudomonas spp. iso lates were identified as P. orientalis (4) and P. fluorescens (2). Only five isolates within three species (B. valezensis, B. 
megaterium and P. orientalis) had both, the activity of the whole culture and supernatant.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Online Conference on Microbiology
T1  - IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA
SP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1950
ER  - 

@conference{
author = "Jelušić, Aleksandra and Berić, Tanja and Dimkić, Ivica and Mitrović, Petar and Stanković, Slaviša and Marković, Sanja and Popović, Tatjana",
year = "2020",
abstract = "Background:
Yield and quality of oilseed rape and other Brassicacceae vegetables are affected with plant pathogenic bacterium 
Xanthomonas campestris pv. campestris (Xcc), both worldwide and in Serbia. Considering the losses that can arise as
a consequence of the infection with this pathogen, there is an increasing need for finding the effective and environ mentally safe method for its suppression.
Objectives:
With the aim to find a promising biological control agent for managing Xcc infection on winter oilseed rape in Serbia, 
this research was based on evaluation of the in vitro antagonistic potential of the Bacillus- and Pseudomonas-like
isolates obtained from the phyllosphere and rhizosphere of the diseased and healthy winter oilseed rape plants.
Methods:
Well diffusion technique was used to evaluate the potential inhibitory activity of the whole cultures and cell-free 
supernatants of the 36 Bacillus- and Pseudomonas-like isolates, against the collection of Serbian winter oilseed rape 
Xcc isolates. DNA of the isolates which were able to inhibit Xcc growth was amplified with the primers based on the 
sequences of tuf and gyrB genes for Bacillus- and Pseudomonas-like isolates, respectively and sequenced.
Results:
Nine Bacillus spp. isolates whose whole cultures showed antagonistic potential were identified as B. thuringiensis/B. 
cereus (5); B. valezensis (1); B. mobilis (1); B. megaterium (1) and B. halotolerans (1), while six Pseudomonas spp. iso lates were identified as P. orientalis (4) and P. fluorescens (2). Only five isolates within three species (B. valezensis, B. 
megaterium and P. orientalis) had both, the activity of the whole culture and supernatant.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Online Conference on Microbiology",
title = "IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA",
pages = "133",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1950"
}

Jelušić, A., Berić, T., Dimkić, I., Mitrović, P., Stanković, S., Marković, S.,& Popović, T.. (2020). IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA. in FEMS Online Conference on Microbiology
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology., 133.
https://hdl.handle.net/21.15107/rcub_rimsi_1950

Jelušić A, Berić T, Dimkić I, Mitrović P, Stanković S, Marković S, Popović T. IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA. in FEMS Online Conference on Microbiology. 2020;:133.
https://hdl.handle.net/21.15107/rcub_rimsi_1950 .

Jelušić, Aleksandra, Berić, Tanja, Dimkić, Ivica, Mitrović, Petar, Stanković, Slaviša, Marković, Sanja, Popović, Tatjana, "IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA" in FEMS Online Conference on Microbiology (2020):133,
https://hdl.handle.net/21.15107/rcub_rimsi_1950 .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
AU  - Dimkić, Ivica
AU  - Mitrović, Petar
AU  - Stanković, Slaviša
AU  - Marković, Sanja
AU  - Berić, Tanja
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1954
AB  - Species belonging to the genus Bacillus are known for their ability to suppress various plant pathogenic fungi and 
bacteria, due to synthesis of secondary metabolites like antibiotics and lipopeptides. The main lipopeptide families 
responsible for the antimicrobial activity of Bacillus species are fengycins, iturins, kurstakins and surfactins. 
Objectives:
The aim of this study was to identify and test two Bacillus-like isolates which showed in vitro antagonistic potential 
against phytopathogenic bacterium Xanthomonas campestris pv. campestris for the presence of lipopeptide genes. 
Methods:
For identification, DNA of tested isolates was amplified with primers based on 16S rRNA and tuf genes and sequenced. 
The presence of lipopeptide genes was detected using primer pairs ITUP1-F/ITUP1-R, AKS-F/TKS-R, FEND1-F/FEND1-R, 
BACC1-F/BACC1 and P17/P18 for detection of the genes for iturin, kurstakin, fengycin and bacillomycin D synthetases 
and surfactin, respectively. The obtained PCR products were checked for correct size and sequenced. The liquid chro matography-mass spectrometry (LC-MS) was used to confirm presence of the searched lipopeptides.
Results:
Based on the obtained sequences for 16S rRNA and tuf gene, isolates were identified as Bacillus valezensis and Bacillus 
megaterium. The results of PCR screening showed that both strains have genes for biosynthesis of kurstakines and 
surfactines, based on the obtained band of correct size (1125–1175 bp and 675 bp, respectively). B. valezensis strain 
showed presence of the gene for bacillomycin D synthetase, producing the band on the position of 800 bp. 
Presence of these molecules was also confirmed with the LC-MS.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Online Conference on Microbiology
T1  - PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS
SP  - 134
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1954
ER  - 

@conference{
author = "Jelušić, Aleksandra and Popović, Tatjana and Dimkić, Ivica and Mitrović, Petar and Stanković, Slaviša and Marković, Sanja and Berić, Tanja",
year = "2020",
abstract = "Species belonging to the genus Bacillus are known for their ability to suppress various plant pathogenic fungi and 
bacteria, due to synthesis of secondary metabolites like antibiotics and lipopeptides. The main lipopeptide families 
responsible for the antimicrobial activity of Bacillus species are fengycins, iturins, kurstakins and surfactins. 
Objectives:
The aim of this study was to identify and test two Bacillus-like isolates which showed in vitro antagonistic potential 
against phytopathogenic bacterium Xanthomonas campestris pv. campestris for the presence of lipopeptide genes. 
Methods:
For identification, DNA of tested isolates was amplified with primers based on 16S rRNA and tuf genes and sequenced. 
The presence of lipopeptide genes was detected using primer pairs ITUP1-F/ITUP1-R, AKS-F/TKS-R, FEND1-F/FEND1-R, 
BACC1-F/BACC1 and P17/P18 for detection of the genes for iturin, kurstakin, fengycin and bacillomycin D synthetases 
and surfactin, respectively. The obtained PCR products were checked for correct size and sequenced. The liquid chro matography-mass spectrometry (LC-MS) was used to confirm presence of the searched lipopeptides.
Results:
Based on the obtained sequences for 16S rRNA and tuf gene, isolates were identified as Bacillus valezensis and Bacillus 
megaterium. The results of PCR screening showed that both strains have genes for biosynthesis of kurstakines and 
surfactines, based on the obtained band of correct size (1125–1175 bp and 675 bp, respectively). B. valezensis strain 
showed presence of the gene for bacillomycin D synthetase, producing the band on the position of 800 bp. 
Presence of these molecules was also confirmed with the LC-MS.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Online Conference on Microbiology",
title = "PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS",
pages = "134",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1954"
}

Jelušić, A., Popović, T., Dimkić, I., Mitrović, P., Stanković, S., Marković, S.,& Berić, T.. (2020). PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS. in FEMS Online Conference on Microbiology
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology., 134.
https://hdl.handle.net/21.15107/rcub_rimsi_1954

Jelušić A, Popović T, Dimkić I, Mitrović P, Stanković S, Marković S, Berić T. PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS. in FEMS Online Conference on Microbiology. 2020;:134.
https://hdl.handle.net/21.15107/rcub_rimsi_1954 .

Jelušić, Aleksandra, Popović, Tatjana, Dimkić, Ivica, Mitrović, Petar, Stanković, Slaviša, Marković, Sanja, Berić, Tanja, "PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS" in FEMS Online Conference on Microbiology (2020):134,
https://hdl.handle.net/21.15107/rcub_rimsi_1954 .

TY  - CONF
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Marković, Sanja
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1966
AB  - Cabbage (Brassica oleracea var. capitata L.) is one of the most important vegetable crop in Ser bia, mainly significant for its richness in beneficial nutrients, both minerals and vitamins. Its yield can 
nowadays be reduced due to presence of many phytopathogens. Xanthomonas campestris pv. camp estris (Xcc), a causal agent of black rot disease, is among the most significant bacteria which affects 
proper cabbage growth, leading to loss in head weight and quality, therefore drastically reducing 
its economic value. This pathogen is genetically diverse, which can be seen through the worldwide 
presence of eleven races and more than thirty combinations of allelic profiles of just four housekeep ing genes - dnaK, fyuA, gyrB, rpoD. The main goal of this work was to subject Xcc isolates originated 
from cabbage to sequence analysis and to determine their affiliation to certain allelic group. 
Forty-four Xcc isolates obtained from cabbage in 2014, from five different localities in Serbia 
(Futog, Temerin, Šabac, Vrnjačka Banja, Čačak), were taken for analysis. DNA of all isolates was first 
amplified using BOXA1R rep-PCR primer to check if differences in profiles exist. Representative iso lates were then sequenced on the basis of four housekeeping genes (dnaK, fyuA, gyrB, rpoD). Non-re dundatant database (NRDB) from pubMLST (https://pubmlst.org/analysis/nrdb.shtml) was used to 
determine the allelic profiles for each of the sequenced genes, and to compare tested isolates with 
already described and deposited strains from NCBI database, whose allelic profiles are known. 
The results of rep-PCR fingerprinting revealed the same banding patterns for all tested isolates. 
The allelic profile was 1, 3, 1, 1 for dnaK, fyuA, gyrB and rpoD gene, respectively. This profile coincides 
with Portuguese B. oleracea Xcc strains CPBF 213, CPBF 1126 (race 6) and CPBF 331 (race 10). The ob tained allelic profile is assigned as sequence type 2 (ST2). In conclusion, since different races can have 
same allelic profile combinations and vice versa, Xcc race affiliation cannot be determined based on 
their genetic differences.
PB  - Plant Protection Society of Serbia
C3  - VIII CONGRESS ON PLANT  PROTECTION:  Integrated Plant Protection for Sustainable Crop Production and Forestry
T1  - DETERMINATION OF ALLELIC PROFILE OF Xanthomonas campestris pv.  campestris ISOLATES ORIGINATED FROM CABBAGE
SP  - 186
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1966
ER  - 

@conference{
author = "Popović, Tatjana and Jelušić, Aleksandra and Mitrović, Petar and Iličić, Renata and Marković, Sanja",
year = "2019",
abstract = "Cabbage (Brassica oleracea var. capitata L.) is one of the most important vegetable crop in Ser bia, mainly significant for its richness in beneficial nutrients, both minerals and vitamins. Its yield can 
nowadays be reduced due to presence of many phytopathogens. Xanthomonas campestris pv. camp estris (Xcc), a causal agent of black rot disease, is among the most significant bacteria which affects 
proper cabbage growth, leading to loss in head weight and quality, therefore drastically reducing 
its economic value. This pathogen is genetically diverse, which can be seen through the worldwide 
presence of eleven races and more than thirty combinations of allelic profiles of just four housekeep ing genes - dnaK, fyuA, gyrB, rpoD. The main goal of this work was to subject Xcc isolates originated 
from cabbage to sequence analysis and to determine their affiliation to certain allelic group. 
Forty-four Xcc isolates obtained from cabbage in 2014, from five different localities in Serbia 
(Futog, Temerin, Šabac, Vrnjačka Banja, Čačak), were taken for analysis. DNA of all isolates was first 
amplified using BOXA1R rep-PCR primer to check if differences in profiles exist. Representative iso lates were then sequenced on the basis of four housekeeping genes (dnaK, fyuA, gyrB, rpoD). Non-re dundatant database (NRDB) from pubMLST (https://pubmlst.org/analysis/nrdb.shtml) was used to 
determine the allelic profiles for each of the sequenced genes, and to compare tested isolates with 
already described and deposited strains from NCBI database, whose allelic profiles are known. 
The results of rep-PCR fingerprinting revealed the same banding patterns for all tested isolates. 
The allelic profile was 1, 3, 1, 1 for dnaK, fyuA, gyrB and rpoD gene, respectively. This profile coincides 
with Portuguese B. oleracea Xcc strains CPBF 213, CPBF 1126 (race 6) and CPBF 331 (race 10). The ob tained allelic profile is assigned as sequence type 2 (ST2). In conclusion, since different races can have 
same allelic profile combinations and vice versa, Xcc race affiliation cannot be determined based on 
their genetic differences.",
publisher = "Plant Protection Society of Serbia",
journal = "VIII CONGRESS ON PLANT  PROTECTION:  Integrated Plant Protection for Sustainable Crop Production and Forestry",
title = "DETERMINATION OF ALLELIC PROFILE OF Xanthomonas campestris pv.  campestris ISOLATES ORIGINATED FROM CABBAGE",
pages = "186",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1966"
}

Popović, T., Jelušić, A., Mitrović, P., Iličić, R.,& Marković, S.. (2019). DETERMINATION OF ALLELIC PROFILE OF Xanthomonas campestris pv.  campestris ISOLATES ORIGINATED FROM CABBAGE. in VIII CONGRESS ON PLANT  PROTECTION:  Integrated Plant Protection for Sustainable Crop Production and Forestry
Plant Protection Society of Serbia., 186.
https://hdl.handle.net/21.15107/rcub_rimsi_1966

Popović T, Jelušić A, Mitrović P, Iličić R, Marković S. DETERMINATION OF ALLELIC PROFILE OF Xanthomonas campestris pv.  campestris ISOLATES ORIGINATED FROM CABBAGE. in VIII CONGRESS ON PLANT  PROTECTION:  Integrated Plant Protection for Sustainable Crop Production and Forestry. 2019;:186.
https://hdl.handle.net/21.15107/rcub_rimsi_1966 .

Popović, Tatjana, Jelušić, Aleksandra, Mitrović, Petar, Iličić, Renata, Marković, Sanja, "DETERMINATION OF ALLELIC PROFILE OF Xanthomonas campestris pv.  campestris ISOLATES ORIGINATED FROM CABBAGE" in VIII CONGRESS ON PLANT  PROTECTION:  Integrated Plant Protection for Sustainable Crop Production and Forestry (2019):186,
https://hdl.handle.net/21.15107/rcub_rimsi_1966 .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
AU  - Mitrović, Petar
AU  - Dimkić, Ivica
AU  - Stanković, Slaviša
AU  - Marković, Sanja
AU  - Berić, Tanja
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1933
AB  - Background: Oilseed rape (Brassica napus) is an oil-producing crop, significant for vegetable oil and 
biodiesel production. The phytopathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) 
causing bacterial leaf blight was recorded for a first time in 2010 on self-growing winter oilseed rape 
in Serbia. Ever since, it continued appearing on cultivated crops too. 
Objectives: The aim of this research was to reveal genetic heterogeneity among 25 Xcc isolates, 
originated from different cultivars of winter oilseed rape obtained in nine-year period (2010-2018) 
from different localities in Vojvodina region (Serbia) using repetitive sequence-based PCR (rep-PCR) 
fingerprinting. 
Methods: Genomic DNA was extracted using CTAB method. Specific primer pair XCF/XCR, amplifying 
a DNA fragment of hrpF gene from X. campestris was used for rapid preliminary identification of all 
tested isolates. Rep-PCR using BOX, ERIC and REP-PCR primers was used to detect presence of 
genetic heterogeneity among Xcc isolates.
Results: All 25 tested isolates were preliminary identified as Xcc based on a positive PCR reaction 
after amplification of 525 bp region using XCF/XCR primers. BOX and ERIC-PCR generated seventeen 
different DNA fingerprinting profiles, unlike REP-PCR where only nine different profiles were 
detected. These results show presence of genetic polymorphism among Xcc isolates from winter 
oilseed rape, which could indicate either their affiliation to some of the identified Xcc races, or 
formation of a new Xcc ecotype.
PB  - Federation of European Microbiological Societies
C3  - 8th Congress of  European Microbiologists
T1  - Genetic heterogeneity among Xanthomonas campestris pv. campestris isolates originated  from oilseed rape determined with different rep-PCR techniques
SP  - 991
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1933
ER  - 

@conference{
author = "Jelušić, Aleksandra and Popović, Tatjana and Mitrović, Petar and Dimkić, Ivica and Stanković, Slaviša and Marković, Sanja and Berić, Tanja",
year = "2019",
abstract = "Background: Oilseed rape (Brassica napus) is an oil-producing crop, significant for vegetable oil and 
biodiesel production. The phytopathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) 
causing bacterial leaf blight was recorded for a first time in 2010 on self-growing winter oilseed rape 
in Serbia. Ever since, it continued appearing on cultivated crops too. 
Objectives: The aim of this research was to reveal genetic heterogeneity among 25 Xcc isolates, 
originated from different cultivars of winter oilseed rape obtained in nine-year period (2010-2018) 
from different localities in Vojvodina region (Serbia) using repetitive sequence-based PCR (rep-PCR) 
fingerprinting. 
Methods: Genomic DNA was extracted using CTAB method. Specific primer pair XCF/XCR, amplifying 
a DNA fragment of hrpF gene from X. campestris was used for rapid preliminary identification of all 
tested isolates. Rep-PCR using BOX, ERIC and REP-PCR primers was used to detect presence of 
genetic heterogeneity among Xcc isolates.
Results: All 25 tested isolates were preliminary identified as Xcc based on a positive PCR reaction 
after amplification of 525 bp region using XCF/XCR primers. BOX and ERIC-PCR generated seventeen 
different DNA fingerprinting profiles, unlike REP-PCR where only nine different profiles were 
detected. These results show presence of genetic polymorphism among Xcc isolates from winter 
oilseed rape, which could indicate either their affiliation to some of the identified Xcc races, or 
formation of a new Xcc ecotype.",
publisher = "Federation of European Microbiological Societies",
journal = "8th Congress of  European Microbiologists",
title = "Genetic heterogeneity among Xanthomonas campestris pv. campestris isolates originated  from oilseed rape determined with different rep-PCR techniques",
pages = "991",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1933"
}

Jelušić, A., Popović, T., Mitrović, P., Dimkić, I., Stanković, S., Marković, S.,& Berić, T.. (2019). Genetic heterogeneity among Xanthomonas campestris pv. campestris isolates originated  from oilseed rape determined with different rep-PCR techniques. in 8th Congress of  European Microbiologists
Federation of European Microbiological Societies., 991.
https://hdl.handle.net/21.15107/rcub_rimsi_1933

Jelušić A, Popović T, Mitrović P, Dimkić I, Stanković S, Marković S, Berić T. Genetic heterogeneity among Xanthomonas campestris pv. campestris isolates originated  from oilseed rape determined with different rep-PCR techniques. in 8th Congress of  European Microbiologists. 2019;:991.
https://hdl.handle.net/21.15107/rcub_rimsi_1933 .

Jelušić, Aleksandra, Popović, Tatjana, Mitrović, Petar, Dimkić, Ivica, Stanković, Slaviša, Marković, Sanja, Berić, Tanja, "Genetic heterogeneity among Xanthomonas campestris pv. campestris isolates originated  from oilseed rape determined with different rep-PCR techniques" in 8th Congress of  European Microbiologists (2019):991,
https://hdl.handle.net/21.15107/rcub_rimsi_1933 .