TY  - JOUR
AU  - Simonović, Mladen
AU  - Kojić, Vesna
AU  - Jakimov, Dimitar
AU  - Glumac, Miodrag
AU  - Pejin, Boris
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1513
AB  - This study was focused on in vitro cytotoxicity screening of the raspberry seeds methanol extract towards a number of cancer cell lines of human origin. The tested extract at the preferred concentrations (IC50  lt 30 mu g/mL) inhibited only the growth of the lung cancer A-549 cells (IC50 = 14.07 +/- 0.96 mu g/mL). At the same time, it was practically inactive (IC50 >300 mu g/mL) and non-mutagenic towards normal MRC-5 lung cells. Finally, the extract potently scavenged both OH center dot (IC50 = 20.11 +/- 1.77 mu g/mL) and O2-center dot (IC50 = 47.23 +/- 3.82 mu g/mL), the free radicals of proved relevance for cancer pathophysiology. Though seeds were enriched with phenolic compounds (TPC = 5.21 +/- 0.07 mg GAE/g), anthocyanins were present in traces only (TAC = 0.07 +/- 0.003 mg cyn-3-glu/g), while flavonoids were not detected at all. This is the first report on anti-lung cancer potential of the seeds of any soft fruit.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - Raspberry seeds extract selectively inhibits the growth of human lung cancer cells in vitro
EP  - 2256
IS  - 13
SP  - 2253
VL  - 35
DO  - 10.1080/14786419.2019.1666391
ER  - 

@article{
author = "Simonović, Mladen and Kojić, Vesna and Jakimov, Dimitar and Glumac, Miodrag and Pejin, Boris",
year = "2021",
abstract = "This study was focused on in vitro cytotoxicity screening of the raspberry seeds methanol extract towards a number of cancer cell lines of human origin. The tested extract at the preferred concentrations (IC50  lt 30 mu g/mL) inhibited only the growth of the lung cancer A-549 cells (IC50 = 14.07 +/- 0.96 mu g/mL). At the same time, it was practically inactive (IC50 >300 mu g/mL) and non-mutagenic towards normal MRC-5 lung cells. Finally, the extract potently scavenged both OH center dot (IC50 = 20.11 +/- 1.77 mu g/mL) and O2-center dot (IC50 = 47.23 +/- 3.82 mu g/mL), the free radicals of proved relevance for cancer pathophysiology. Though seeds were enriched with phenolic compounds (TPC = 5.21 +/- 0.07 mg GAE/g), anthocyanins were present in traces only (TAC = 0.07 +/- 0.003 mg cyn-3-glu/g), while flavonoids were not detected at all. This is the first report on anti-lung cancer potential of the seeds of any soft fruit.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "Raspberry seeds extract selectively inhibits the growth of human lung cancer cells in vitro",
pages = "2256-2253",
number = "13",
volume = "35",
doi = "10.1080/14786419.2019.1666391"
}

Simonović, M., Kojić, V., Jakimov, D., Glumac, M.,& Pejin, B.. (2021). Raspberry seeds extract selectively inhibits the growth of human lung cancer cells in vitro. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 35(13), 2253-2256.
https://doi.org/10.1080/14786419.2019.1666391

Simonović M, Kojić V, Jakimov D, Glumac M, Pejin B. Raspberry seeds extract selectively inhibits the growth of human lung cancer cells in vitro. in Natural Product Research. 2021;35(13):2253-2256.
doi:10.1080/14786419.2019.1666391 .

Simonović, Mladen, Kojić, Vesna, Jakimov, Dimitar, Glumac, Miodrag, Pejin, Boris, "Raspberry seeds extract selectively inhibits the growth of human lung cancer cells in vitro" in Natural Product Research, 35, no. 13 (2021):2253-2256,
https://doi.org/10.1080/14786419.2019.1666391 . .

TY  - JOUR
AU  - Pejin, Boris
AU  - Simonović, Mladen
AU  - Talevska, Aleksandra
AU  - Glumac, Miodrag
AU  - Jakimov, Dimitar
AU  - Kojić, Vesna
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1511
AB  - The cytotoxicity of the methanol extract of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937) (Malawispongiidae) was evaluated by MTT assay at in vitro conditions against three brain tumour cell lines (Neuro-2A, U-251 MG and U-87 MG). The extract was actually found to be most effective against the malignant glioma U-251 MG cells reaching a promising IC50 value of 1.87 +/- 0.09 mu g/mL at 96 h. However, it exhibited only a bit of cytotoxicity (IC50 321.14 +/- 11.29 mu g/mL, 96 h) towards the normal cells. Also, this sponge extract was 5-fold more selective for U-251 MG versus U-87 MG cells. Finally, monitoring genotoxicity at chromosomal level using the micronucleus test practically revealed lack of any significant toxicity of O. rotunda extract, compared to doxorubicin. [GRAPHICS] .
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - A neglected natural source for targeting glioblastoma
EP  - 1860
IS  - 11
SP  - 1856
VL  - 35
DO  - 10.1080/14786419.2019.1638386
ER  - 

@article{
author = "Pejin, Boris and Simonović, Mladen and Talevska, Aleksandra and Glumac, Miodrag and Jakimov, Dimitar and Kojić, Vesna",
year = "2021",
abstract = "The cytotoxicity of the methanol extract of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937) (Malawispongiidae) was evaluated by MTT assay at in vitro conditions against three brain tumour cell lines (Neuro-2A, U-251 MG and U-87 MG). The extract was actually found to be most effective against the malignant glioma U-251 MG cells reaching a promising IC50 value of 1.87 +/- 0.09 mu g/mL at 96 h. However, it exhibited only a bit of cytotoxicity (IC50 321.14 +/- 11.29 mu g/mL, 96 h) towards the normal cells. Also, this sponge extract was 5-fold more selective for U-251 MG versus U-87 MG cells. Finally, monitoring genotoxicity at chromosomal level using the micronucleus test practically revealed lack of any significant toxicity of O. rotunda extract, compared to doxorubicin. [GRAPHICS] .",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "A neglected natural source for targeting glioblastoma",
pages = "1860-1856",
number = "11",
volume = "35",
doi = "10.1080/14786419.2019.1638386"
}

Pejin, B., Simonović, M., Talevska, A., Glumac, M., Jakimov, D.,& Kojić, V.. (2021). A neglected natural source for targeting glioblastoma. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 35(11), 1856-1860.
https://doi.org/10.1080/14786419.2019.1638386

Pejin B, Simonović M, Talevska A, Glumac M, Jakimov D, Kojić V. A neglected natural source for targeting glioblastoma. in Natural Product Research. 2021;35(11):1856-1860.
doi:10.1080/14786419.2019.1638386 .

Pejin, Boris, Simonović, Mladen, Talevska, Aleksandra, Glumac, Miodrag, Jakimov, Dimitar, Kojić, Vesna, "A neglected natural source for targeting glioblastoma" in Natural Product Research, 35, no. 11 (2021):1856-1860,
https://doi.org/10.1080/14786419.2019.1638386 . .

TY  - JOUR
AU  - Talevska, Aleksandra
AU  - Pejin, Boris
AU  - Kojić, Vesna
AU  - Berić, Tanja
AU  - Stanković, Slaviša
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1149
AB  - In vitro anti-tumour and anti-radical activities of the acetone extract of the freshwater sponge Ochridaspongia rotunda were the subject of this study. The extract was found to be highly cytotoxic to human lung tumour cell line A-549 reaching IC50 value of 5.01 +/- 0.21 mu g/mL. Indeed, it displayed only 2-fold less anti-tumour activity than doxorubicin (IC50 value 2.42 +/- 0.13 mu g/mL) used as a positive control. The same extract was also found to be almost 37-fold more selective against A-549 vs. MRC-5 (normal) lung cells, in difference to weak selectivity of doxorubicin (less than 3-fold). Its profound anti-DPPH radical activity comparable to that of quercetin (IC50 values 3.68 +/- 0.19 and 3.14 +/- 0.09 mu g/mL, respectively) coupled with no signs of genotoxicity in the comet assay (MRC-5 cell line, vs. doxorubicin) has actually implicated the importance of this animal bioresource in searching for pharmaceutically useful bioactive compounds of natural origin. [GRAPHICS] .
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - A contribution to pharmaceutical biology of freshwater sponges
EP  - 571
IS  - 5
SP  - 568
VL  - 32
DO  - 10.1080/14786419.2017.1315719
ER  - 

@article{
author = "Talevska, Aleksandra and Pejin, Boris and Kojić, Vesna and Berić, Tanja and Stanković, Slaviša",
year = "2018",
abstract = "In vitro anti-tumour and anti-radical activities of the acetone extract of the freshwater sponge Ochridaspongia rotunda were the subject of this study. The extract was found to be highly cytotoxic to human lung tumour cell line A-549 reaching IC50 value of 5.01 +/- 0.21 mu g/mL. Indeed, it displayed only 2-fold less anti-tumour activity than doxorubicin (IC50 value 2.42 +/- 0.13 mu g/mL) used as a positive control. The same extract was also found to be almost 37-fold more selective against A-549 vs. MRC-5 (normal) lung cells, in difference to weak selectivity of doxorubicin (less than 3-fold). Its profound anti-DPPH radical activity comparable to that of quercetin (IC50 values 3.68 +/- 0.19 and 3.14 +/- 0.09 mu g/mL, respectively) coupled with no signs of genotoxicity in the comet assay (MRC-5 cell line, vs. doxorubicin) has actually implicated the importance of this animal bioresource in searching for pharmaceutically useful bioactive compounds of natural origin. [GRAPHICS] .",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "A contribution to pharmaceutical biology of freshwater sponges",
pages = "571-568",
number = "5",
volume = "32",
doi = "10.1080/14786419.2017.1315719"
}

Talevska, A., Pejin, B., Kojić, V., Berić, T.,& Stanković, S.. (2018). A contribution to pharmaceutical biology of freshwater sponges. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 32(5), 568-571.
https://doi.org/10.1080/14786419.2017.1315719

Talevska A, Pejin B, Kojić V, Berić T, Stanković S. A contribution to pharmaceutical biology of freshwater sponges. in Natural Product Research. 2018;32(5):568-571.
doi:10.1080/14786419.2017.1315719 .

Talevska, Aleksandra, Pejin, Boris, Kojić, Vesna, Berić, Tanja, Stanković, Slaviša, "A contribution to pharmaceutical biology of freshwater sponges" in Natural Product Research, 32, no. 5 (2018):568-571,
https://doi.org/10.1080/14786419.2017.1315719 . .

TY  - JOUR
AU  - Pejin, Boris
AU  - Tommonaro, Giuseppina
AU  - Glumac, Miodrag
AU  - Jakimov, Dimitar
AU  - Kojić, Vesna
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1194
AB  - This study aimed to screen in vitro antitumour activity of the redox couple avarol/avarone against the human malignant glioma cell line U-251 MG for the first time. Compared both with avarol and positive controls used (temozolomide and doxorubicin), avarone was found to be the most active compound with IC50 value below 1 mu M (IC50 0.68 +/- 0.04 mu M, 96 h). Considerable less DNA damage in the cells treated with avarol and avarone vs. doxorubicin (105 & 123% vs. 299%, respectively; untreated U-251 MG cells were used as a control, 100%), coupled with no sign of cytotoxicity against the normal human foetal lung fibroblast MRC-5 cells (IC50 > 100 mu M), has actually pointed out the importance of this marine sesquiterpenoid quinone structure as a promising lead compound in development of novel brain chemotherapeutics. [GRAPHICS] .
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - The redox couple avarol/avarone in the fight with malignant gliomas: the case study of U-251 MG cells
EP  - 620
IS  - 5
SP  - 616
VL  - 32
DO  - 10.1080/14786419.2017.1327959
ER  - 

@article{
author = "Pejin, Boris and Tommonaro, Giuseppina and Glumac, Miodrag and Jakimov, Dimitar and Kojić, Vesna",
year = "2018",
abstract = "This study aimed to screen in vitro antitumour activity of the redox couple avarol/avarone against the human malignant glioma cell line U-251 MG for the first time. Compared both with avarol and positive controls used (temozolomide and doxorubicin), avarone was found to be the most active compound with IC50 value below 1 mu M (IC50 0.68 +/- 0.04 mu M, 96 h). Considerable less DNA damage in the cells treated with avarol and avarone vs. doxorubicin (105 & 123% vs. 299%, respectively; untreated U-251 MG cells were used as a control, 100%), coupled with no sign of cytotoxicity against the normal human foetal lung fibroblast MRC-5 cells (IC50 > 100 mu M), has actually pointed out the importance of this marine sesquiterpenoid quinone structure as a promising lead compound in development of novel brain chemotherapeutics. [GRAPHICS] .",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "The redox couple avarol/avarone in the fight with malignant gliomas: the case study of U-251 MG cells",
pages = "620-616",
number = "5",
volume = "32",
doi = "10.1080/14786419.2017.1327959"
}

Pejin, B., Tommonaro, G., Glumac, M., Jakimov, D.,& Kojić, V.. (2018). The redox couple avarol/avarone in the fight with malignant gliomas: the case study of U-251 MG cells. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 32(5), 616-620.
https://doi.org/10.1080/14786419.2017.1327959

Pejin B, Tommonaro G, Glumac M, Jakimov D, Kojić V. The redox couple avarol/avarone in the fight with malignant gliomas: the case study of U-251 MG cells. in Natural Product Research. 2018;32(5):616-620.
doi:10.1080/14786419.2017.1327959 .

Pejin, Boris, Tommonaro, Giuseppina, Glumac, Miodrag, Jakimov, Dimitar, Kojić, Vesna, "The redox couple avarol/avarone in the fight with malignant gliomas: the case study of U-251 MG cells" in Natural Product Research, 32, no. 5 (2018):616-620,
https://doi.org/10.1080/14786419.2017.1327959 . .

TY  - JOUR
AU  - Pejin, Boris
AU  - Iodice, Carmine
AU  - Bogdanović, Gordana
AU  - Kojić, Vesna
AU  - Tešević, Vele
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1092
AB  - The growth inhibition of stictic acid, a secondary metabolite isolated from the lichen Lobaria pulmonaria (L.) Hoffm. (Lobariaceae), was evaluated in vitro on three human cell lines for the first time. The cell lines HT-29 and MCF-7 were utilized for measuring the activity of stictic acid against cancer cells, while the cell line MRC-5 was selected for estimation of its effect on normal cells. The results suggest a moderate anticancer activity (IC50 value for the cell line HT-29 was 29.29 mu g/ml) and a low growth inhibition on nonmalignant cells (IC50 value for the cell line MRC-5 was 2478.40 mu g/ml) of stictic acid. This natural product can be considered as a promising lead compound for the design of novel human colon adenocarcinoma drugs.
PB  - Elsevier Science Bv, Amsterdam
T2  - Arabian Journal of Chemistry
T1  - Stictic acid inhibits cell growth of human colon adenocarcinoma HT-29 cells
EP  - S1242
SP  - S1240
VL  - 10
DO  - 10.1016/j.arabjc.2013.03.003
ER  - 

@article{
author = "Pejin, Boris and Iodice, Carmine and Bogdanović, Gordana and Kojić, Vesna and Tešević, Vele",
year = "2017",
abstract = "The growth inhibition of stictic acid, a secondary metabolite isolated from the lichen Lobaria pulmonaria (L.) Hoffm. (Lobariaceae), was evaluated in vitro on three human cell lines for the first time. The cell lines HT-29 and MCF-7 were utilized for measuring the activity of stictic acid against cancer cells, while the cell line MRC-5 was selected for estimation of its effect on normal cells. The results suggest a moderate anticancer activity (IC50 value for the cell line HT-29 was 29.29 mu g/ml) and a low growth inhibition on nonmalignant cells (IC50 value for the cell line MRC-5 was 2478.40 mu g/ml) of stictic acid. This natural product can be considered as a promising lead compound for the design of novel human colon adenocarcinoma drugs.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Arabian Journal of Chemistry",
title = "Stictic acid inhibits cell growth of human colon adenocarcinoma HT-29 cells",
pages = "S1242-S1240",
volume = "10",
doi = "10.1016/j.arabjc.2013.03.003"
}

Pejin, B., Iodice, C., Bogdanović, G., Kojić, V.,& Tešević, V.. (2017). Stictic acid inhibits cell growth of human colon adenocarcinoma HT-29 cells. in Arabian Journal of Chemistry
Elsevier Science Bv, Amsterdam., 10, S1240-S1242.
https://doi.org/10.1016/j.arabjc.2013.03.003

Pejin B, Iodice C, Bogdanović G, Kojić V, Tešević V. Stictic acid inhibits cell growth of human colon adenocarcinoma HT-29 cells. in Arabian Journal of Chemistry. 2017;10:S1240-S1242.
doi:10.1016/j.arabjc.2013.03.003 .

Pejin, Boris, Iodice, Carmine, Bogdanović, Gordana, Kojić, Vesna, Tešević, Vele, "Stictic acid inhibits cell growth of human colon adenocarcinoma HT-29 cells" in Arabian Journal of Chemistry, 10 (2017):S1240-S1242,
https://doi.org/10.1016/j.arabjc.2013.03.003 . .

TY  - JOUR
AU  - Pejin, Boris
AU  - Iodice, Carmine
AU  - Kojić, Vesna
AU  - Jakimov, Dimitar
AU  - Lazović, Milica
AU  - Tommonaro, Giuseppina
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/953
AB  - The cytotoxicity of avarol, a main secondary metabolite of the Mediterranean sponge Dysidea avara, was in vitro screened by MTT assay against four human tumour cell lines. The colon HT-29 tumour cells practically showed to be the only sensitive ones towards this organic compound. No toxicity was found against the fetal lung fibroblast MRC-5 cells at the concentrations tested. In comparison with doxorubicin, used as a positive control, avarol actually exhibited at least 588-fold less toxicity towards normal MRC-5 cells. Finally, comet assay indicated that DNA fragmentation was almost fivefold higher upon the treatment with doxorubicin, compared to avarol. The obtained results have actually confirmed that avarol scaffold may contribute to development of new cytostatics inspired by nature.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - In vitro evaluation of cytotoxic and mutagenic activity of avarol
EP  - 1296
IS  - 11
SP  - 1293
VL  - 30
DO  - 10.1080/14786419.2015.1052067
ER  - 

@article{
author = "Pejin, Boris and Iodice, Carmine and Kojić, Vesna and Jakimov, Dimitar and Lazović, Milica and Tommonaro, Giuseppina",
year = "2016",
abstract = "The cytotoxicity of avarol, a main secondary metabolite of the Mediterranean sponge Dysidea avara, was in vitro screened by MTT assay against four human tumour cell lines. The colon HT-29 tumour cells practically showed to be the only sensitive ones towards this organic compound. No toxicity was found against the fetal lung fibroblast MRC-5 cells at the concentrations tested. In comparison with doxorubicin, used as a positive control, avarol actually exhibited at least 588-fold less toxicity towards normal MRC-5 cells. Finally, comet assay indicated that DNA fragmentation was almost fivefold higher upon the treatment with doxorubicin, compared to avarol. The obtained results have actually confirmed that avarol scaffold may contribute to development of new cytostatics inspired by nature.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "In vitro evaluation of cytotoxic and mutagenic activity of avarol",
pages = "1296-1293",
number = "11",
volume = "30",
doi = "10.1080/14786419.2015.1052067"
}

Pejin, B., Iodice, C., Kojić, V., Jakimov, D., Lazović, M.,& Tommonaro, G.. (2016). In vitro evaluation of cytotoxic and mutagenic activity of avarol. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 30(11), 1293-1296.
https://doi.org/10.1080/14786419.2015.1052067

Pejin B, Iodice C, Kojić V, Jakimov D, Lazović M, Tommonaro G. In vitro evaluation of cytotoxic and mutagenic activity of avarol. in Natural Product Research. 2016;30(11):1293-1296.
doi:10.1080/14786419.2015.1052067 .

Pejin, Boris, Iodice, Carmine, Kojić, Vesna, Jakimov, Dimitar, Lazović, Milica, Tommonaro, Giuseppina, "In vitro evaluation of cytotoxic and mutagenic activity of avarol" in Natural Product Research, 30, no. 11 (2016):1293-1296,
https://doi.org/10.1080/14786419.2015.1052067 . .

TY  - JOUR
AU  - Pesic, Milica
AU  - Podolski-Renic, Ana
AU  - Stojković, Sonja
AU  - Matović, Branko
AU  - Zmejkoski, Danica
AU  - Kojić, Vesna
AU  - Bogdanović, Gordana
AU  - Pavicević, Aleksandra
AU  - Mojović, Miloš
AU  - Savić, Aleksandar G
AU  - Milenković, Ivana
AU  - Kalauzi, Aleksandar
AU  - Radotić, Ksenija
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/931
AB  - Data on medical applications of cerium oxide nanoparticles CeO2 (CONP) are promising, yet information regarding their action in cells is incomplete and there are conflicting reports about in vitro toxicity. Herein, we have studied cytotoxic effect of CONP in several cancer and normal cell lines and their potential to change intracellular redox status. The IC50 was achieved only in two of eight tested cell lines, melanoma 518A2 and colorectal adenocarcinoma HT-29. Self-propagating room temperature method was applied to produce CONP with an average crystalline size of 4 nm. The results confirmed presence of Ce3+ and O2- vacancies. The induction of cell death by CONP and the production of reactive oxygen species (ROS) were analyzed by flow-cytometry. Free radicals related antioxidant capacity of the cells was studied by the reduction of stable free radical TEMPONE using electron spin resonance spectroscopy. CONP showed low or moderate cytotoxicity in cancer cell lines: adenocarcinoma DLD1 and multi-drug resistant DLD1-TxR, non-small cell lung carcinoma NCI-H460 and multi-drug resistant NCI-H460/R, while normal cell lines (keratinocytes HaCaT, lung fetal fibroblasts MRC-5) were insensitive. The most sensitive were 518A2 melanoma and HT-29 colorectal adenocarcinoma cell lines, with the IC50 values being between 100 and 200 mu M. Decreased rate of TEMPONE reduction and increased production of certain ROS species (peroxynitrite and hydrogen peroxide anion) indicates that free radical metabolism, thus redox status was changed, and antioxidant capacity damaged in the CONP treated 518A2 and HT-29 cells. In conclusion, changes in intracellular redox status induced by CONP are partly attributed to the prooxidant activity of the nanoparticles. Further, ROS induced cell damages might eventually lead to the cell death. However, low inhibitory potential of CONP in the other human cell lines tested indicates that CONP may be safe for human usage in industry and medicine.
PB  - Elsevier Ireland Ltd, Clare
T2  - Chemico-Biological Interactions
T1  - Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity
EP  - 93
SP  - 85
VL  - 232
DO  - 10.1016/j.cbi.2015.03.013
ER  - 

@article{
author = "Pesic, Milica and Podolski-Renic, Ana and Stojković, Sonja and Matović, Branko and Zmejkoski, Danica and Kojić, Vesna and Bogdanović, Gordana and Pavicević, Aleksandra and Mojović, Miloš and Savić, Aleksandar G and Milenković, Ivana and Kalauzi, Aleksandar and Radotić, Ksenija",
year = "2015",
abstract = "Data on medical applications of cerium oxide nanoparticles CeO2 (CONP) are promising, yet information regarding their action in cells is incomplete and there are conflicting reports about in vitro toxicity. Herein, we have studied cytotoxic effect of CONP in several cancer and normal cell lines and their potential to change intracellular redox status. The IC50 was achieved only in two of eight tested cell lines, melanoma 518A2 and colorectal adenocarcinoma HT-29. Self-propagating room temperature method was applied to produce CONP with an average crystalline size of 4 nm. The results confirmed presence of Ce3+ and O2- vacancies. The induction of cell death by CONP and the production of reactive oxygen species (ROS) were analyzed by flow-cytometry. Free radicals related antioxidant capacity of the cells was studied by the reduction of stable free radical TEMPONE using electron spin resonance spectroscopy. CONP showed low or moderate cytotoxicity in cancer cell lines: adenocarcinoma DLD1 and multi-drug resistant DLD1-TxR, non-small cell lung carcinoma NCI-H460 and multi-drug resistant NCI-H460/R, while normal cell lines (keratinocytes HaCaT, lung fetal fibroblasts MRC-5) were insensitive. The most sensitive were 518A2 melanoma and HT-29 colorectal adenocarcinoma cell lines, with the IC50 values being between 100 and 200 mu M. Decreased rate of TEMPONE reduction and increased production of certain ROS species (peroxynitrite and hydrogen peroxide anion) indicates that free radical metabolism, thus redox status was changed, and antioxidant capacity damaged in the CONP treated 518A2 and HT-29 cells. In conclusion, changes in intracellular redox status induced by CONP are partly attributed to the prooxidant activity of the nanoparticles. Further, ROS induced cell damages might eventually lead to the cell death. However, low inhibitory potential of CONP in the other human cell lines tested indicates that CONP may be safe for human usage in industry and medicine.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Chemico-Biological Interactions",
title = "Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity",
pages = "93-85",
volume = "232",
doi = "10.1016/j.cbi.2015.03.013"
}

Pesic, M., Podolski-Renic, A., Stojković, S., Matović, B., Zmejkoski, D., Kojić, V., Bogdanović, G., Pavicević, A., Mojović, M., Savić, A. G., Milenković, I., Kalauzi, A.,& Radotić, K.. (2015). Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity. in Chemico-Biological Interactions
Elsevier Ireland Ltd, Clare., 232, 85-93.
https://doi.org/10.1016/j.cbi.2015.03.013

Pesic M, Podolski-Renic A, Stojković S, Matović B, Zmejkoski D, Kojić V, Bogdanović G, Pavicević A, Mojović M, Savić AG, Milenković I, Kalauzi A, Radotić K. Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity. in Chemico-Biological Interactions. 2015;232:85-93.
doi:10.1016/j.cbi.2015.03.013 .

Pesic, Milica, Podolski-Renic, Ana, Stojković, Sonja, Matović, Branko, Zmejkoski, Danica, Kojić, Vesna, Bogdanović, Gordana, Pavicević, Aleksandra, Mojović, Miloš, Savić, Aleksandar G, Milenković, Ivana, Kalauzi, Aleksandar, Radotić, Ksenija, "Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity" in Chemico-Biological Interactions, 232 (2015):85-93,
https://doi.org/10.1016/j.cbi.2015.03.013 . .

TY  - JOUR
AU  - Pejin, Boris
AU  - Iodice, Carmine
AU  - Tommonaro, Giuseppina
AU  - Bogdanović, Gordana
AU  - Kojić, Vesna 
AU  - De Rosa, Salvatore
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/779
AB  - The cytotoxicity of 4-leucine-avarone, amino derivative of the sponge Dysidea avara secondary metabolite avarone, was evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay in vitro against seven human solid tumours for the first time. The compound tested induced dose-dependent cytotoxic response in all cancer cells showing better activity towards the lung A-549 and colon HT-29 cell lines (IC50 7.40M and 9.62M, respectively) than towards the breast adenocarcinoma ER positive MCF-7 and ER negative MDA-MB-231 cells (IC50 11.64M and 17.31M, respectively), the prostate adenocarcinoma PC-3 and epiteloid cervix carcinoma HeLa cells (IC50 14.24M and 15.54M, respectively). No toxicity was found towards the foetal lung fibroblast MRC-5 cell line at the concentrations used. According to experimental data obtained, the sesquiterpenoid quinone structure of avarone may inspire development of new drug-like substances with improved cytotoxicity on lung cancer in humans.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - Further in vitro evaluation of cytotoxicity of the marine natural product derivative 4 '-leucine-avarone
EP  - 350
IS  - 5
SP  - 347
VL  - 28
DO  - 10.1080/14786419.2013.863201
ER  - 

@article{
author = "Pejin, Boris and Iodice, Carmine and Tommonaro, Giuseppina and Bogdanović, Gordana and Kojić, Vesna  and De Rosa, Salvatore",
year = "2014",
abstract = "The cytotoxicity of 4-leucine-avarone, amino derivative of the sponge Dysidea avara secondary metabolite avarone, was evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay in vitro against seven human solid tumours for the first time. The compound tested induced dose-dependent cytotoxic response in all cancer cells showing better activity towards the lung A-549 and colon HT-29 cell lines (IC50 7.40M and 9.62M, respectively) than towards the breast adenocarcinoma ER positive MCF-7 and ER negative MDA-MB-231 cells (IC50 11.64M and 17.31M, respectively), the prostate adenocarcinoma PC-3 and epiteloid cervix carcinoma HeLa cells (IC50 14.24M and 15.54M, respectively). No toxicity was found towards the foetal lung fibroblast MRC-5 cell line at the concentrations used. According to experimental data obtained, the sesquiterpenoid quinone structure of avarone may inspire development of new drug-like substances with improved cytotoxicity on lung cancer in humans.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "Further in vitro evaluation of cytotoxicity of the marine natural product derivative 4 '-leucine-avarone",
pages = "350-347",
number = "5",
volume = "28",
doi = "10.1080/14786419.2013.863201"
}

Pejin, B., Iodice, C., Tommonaro, G., Bogdanović, G., Kojić, V.,& De Rosa, S.. (2014). Further in vitro evaluation of cytotoxicity of the marine natural product derivative 4 '-leucine-avarone. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 28(5), 347-350.
https://doi.org/10.1080/14786419.2013.863201

Pejin B, Iodice C, Tommonaro G, Bogdanović G, Kojić V, De Rosa S. Further in vitro evaluation of cytotoxicity of the marine natural product derivative 4 '-leucine-avarone. in Natural Product Research. 2014;28(5):347-350.
doi:10.1080/14786419.2013.863201 .

Pejin, Boris, Iodice, Carmine, Tommonaro, Giuseppina, Bogdanović, Gordana, Kojić, Vesna , De Rosa, Salvatore, "Further in vitro evaluation of cytotoxicity of the marine natural product derivative 4 '-leucine-avarone" in Natural Product Research, 28, no. 5 (2014):347-350,
https://doi.org/10.1080/14786419.2013.863201 . .

TY  - JOUR
AU  - Pejin, Boris
AU  - Kojić, Vesna
AU  - Bogdanović, Gordana
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/771
AB  - The cytotoxicity of the diterpene alcohol, phytol, was evaluated by using the MTT assay in vitro against seven tumour cells and one normal cell of human origin. The compound tested induced concentration-dependent cytotoxic response in all cell lines, demonstrating to be most and least effective against the breast adenocarcinoma MCF-7 and the prostate adenocarcinoma PC-3 cells, respectively (IC50 8.79 +/- 0.41 mu M and 77.85 +/- 1.93 mu M). The IC50 values towards the other five tumours (HeLa, HT-29, A-549, Hs294T and MDA-MB-231) ranged from 15.51 to 69.67 mu M. However, mild toxicity was detected against the foetal lung fibroblast MRC-5 cells at the concentrations used (IC50 124.84 +/- 1.59 mu M). According to the experimental data obtained, this cost-effective natural product widely present in the biosphere may inspire the development of new drug-like substances with improved cytotoxic activity on breast cancer.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - An insight into the cytotoxic activity of phytol at in vitro conditions
EP  - 2056
IS  - 22
SP  - 2053
VL  - 28
DO  - 10.1080/14786419.2014.921686
ER  - 

@article{
author = "Pejin, Boris and Kojić, Vesna and Bogdanović, Gordana",
year = "2014",
abstract = "The cytotoxicity of the diterpene alcohol, phytol, was evaluated by using the MTT assay in vitro against seven tumour cells and one normal cell of human origin. The compound tested induced concentration-dependent cytotoxic response in all cell lines, demonstrating to be most and least effective against the breast adenocarcinoma MCF-7 and the prostate adenocarcinoma PC-3 cells, respectively (IC50 8.79 +/- 0.41 mu M and 77.85 +/- 1.93 mu M). The IC50 values towards the other five tumours (HeLa, HT-29, A-549, Hs294T and MDA-MB-231) ranged from 15.51 to 69.67 mu M. However, mild toxicity was detected against the foetal lung fibroblast MRC-5 cells at the concentrations used (IC50 124.84 +/- 1.59 mu M). According to the experimental data obtained, this cost-effective natural product widely present in the biosphere may inspire the development of new drug-like substances with improved cytotoxic activity on breast cancer.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "An insight into the cytotoxic activity of phytol at in vitro conditions",
pages = "2056-2053",
number = "22",
volume = "28",
doi = "10.1080/14786419.2014.921686"
}

Pejin, B., Kojić, V.,& Bogdanović, G.. (2014). An insight into the cytotoxic activity of phytol at in vitro conditions. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 28(22), 2053-2056.
https://doi.org/10.1080/14786419.2014.921686

Pejin B, Kojić V, Bogdanović G. An insight into the cytotoxic activity of phytol at in vitro conditions. in Natural Product Research. 2014;28(22):2053-2056.
doi:10.1080/14786419.2014.921686 .

Pejin, Boris, Kojić, Vesna, Bogdanović, Gordana, "An insight into the cytotoxic activity of phytol at in vitro conditions" in Natural Product Research, 28, no. 22 (2014):2053-2056,
https://doi.org/10.1080/14786419.2014.921686 . .