TY  - JOUR
AU  - Nakarada, Dura
AU  - Pejin, Boris
AU  - Tommonaro, Giuseppina
AU  - Mojović, Miloš
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1340
AB  - The liposomal integration method, in conjunction with electron paramagnetic resonance (EPR) spectroscopy, has been presented for the investigation of antioxidant activity of selected water-insoluble compound towards biologically relevant free radicals. This method was applied to avarol, a sesquiterpenoid hydroquinone isolated from the marine sponge Dysidea avara. The antioxidant activity of water-insoluble avarol towards (OH)-O-center dot, O-2(center dot-) and NO center dot radicals was attained by its incorporation into the DPPC liposomes bilayer, and towards ascorbyl radicals in the organic solvent. Avarol's activity towards (OH)-O-center dot, O-2(center dot-), NO center dot and ascorbyl radicals was 86.2%, 50.9%, 23.6% and 61.8%, respectively, showing its significant radical scavenging potential.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Liposome Research
T1  - Liposomal integration method for assessing antioxidative activity of water insoluble compounds towards biologically relevant free radicals: example of avarol
EP  - 226
IS  - 3
SP  - 218
VL  - 30
DO  - 10.1080/08982104.2019.1625378
ER  - 

@article{
author = "Nakarada, Dura and Pejin, Boris and Tommonaro, Giuseppina and Mojović, Miloš",
year = "2020",
abstract = "The liposomal integration method, in conjunction with electron paramagnetic resonance (EPR) spectroscopy, has been presented for the investigation of antioxidant activity of selected water-insoluble compound towards biologically relevant free radicals. This method was applied to avarol, a sesquiterpenoid hydroquinone isolated from the marine sponge Dysidea avara. The antioxidant activity of water-insoluble avarol towards (OH)-O-center dot, O-2(center dot-) and NO center dot radicals was attained by its incorporation into the DPPC liposomes bilayer, and towards ascorbyl radicals in the organic solvent. Avarol's activity towards (OH)-O-center dot, O-2(center dot-), NO center dot and ascorbyl radicals was 86.2%, 50.9%, 23.6% and 61.8%, respectively, showing its significant radical scavenging potential.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Liposome Research",
title = "Liposomal integration method for assessing antioxidative activity of water insoluble compounds towards biologically relevant free radicals: example of avarol",
pages = "226-218",
number = "3",
volume = "30",
doi = "10.1080/08982104.2019.1625378"
}

Nakarada, D., Pejin, B., Tommonaro, G.,& Mojović, M.. (2020). Liposomal integration method for assessing antioxidative activity of water insoluble compounds towards biologically relevant free radicals: example of avarol. in Journal of Liposome Research
Taylor & Francis Ltd, Abingdon., 30(3), 218-226.
https://doi.org/10.1080/08982104.2019.1625378

Nakarada D, Pejin B, Tommonaro G, Mojović M. Liposomal integration method for assessing antioxidative activity of water insoluble compounds towards biologically relevant free radicals: example of avarol. in Journal of Liposome Research. 2020;30(3):218-226.
doi:10.1080/08982104.2019.1625378 .

Nakarada, Dura, Pejin, Boris, Tommonaro, Giuseppina, Mojović, Miloš, "Liposomal integration method for assessing antioxidative activity of water insoluble compounds towards biologically relevant free radicals: example of avarol" in Journal of Liposome Research, 30, no. 3 (2020):218-226,
https://doi.org/10.1080/08982104.2019.1625378 . .

TY  - JOUR
AU  - Nakarada, Dura
AU  - Pejin, Boris
AU  - Dimic, Dusan
AU  - Ivanovic-Sasic, Ana
AU  - Mojović, Zorica
AU  - Mojović, Miloš
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1206
AB  - The electrochemistry of catecholamine neurotransmitters and their precursor l-dopa has been widely studied due to their relevance as biologically important compounds. The detection of these compounds from aqueous solution is hindered by the coexistence of quinone or hydroquinone. However, it was suggested that quinones adsorbed on the electrode surface can enhance catechol detection. In order to estimate the degree of interaction between quinones and l-dopa, cyclic voltammetry and UV-Vis spectroscopic study was performed. A sesquiterpenoid hydroquinone, isolated from the marine sponge Dysidea avara (avarol), has been used in this study. The change of apparent heterogeneous rate constant with different avarol/l-dopa ratio indicated that charge transfer could be enhanced at some extent. In addition to this, the obtained results for avarol and hydroquinone (its structural element) were compared. UV-Vis spectroscopic analysis confirmed interaction between l-dopa and avarol or hydroquinone. Taken all together, the interaction of l-dopa was stronger with hydroquinone than with avarol, presumably reflecting the conformational restrains of avarol caused by its terpenoid moiety.
PB  - Springer, Dordrecht
T2  - Reaction Kinetics Mechanisms and Catalysis
T1  - Electrochemical and spectroscopic study of l-dopa interaction with avarol
EP  - 229
IS  - 1
SP  - 219
VL  - 127
DO  - 10.1007/s11144-019-01575-z
ER  - 

@article{
author = "Nakarada, Dura and Pejin, Boris and Dimic, Dusan and Ivanovic-Sasic, Ana and Mojović, Zorica and Mojović, Miloš",
year = "2019",
abstract = "The electrochemistry of catecholamine neurotransmitters and their precursor l-dopa has been widely studied due to their relevance as biologically important compounds. The detection of these compounds from aqueous solution is hindered by the coexistence of quinone or hydroquinone. However, it was suggested that quinones adsorbed on the electrode surface can enhance catechol detection. In order to estimate the degree of interaction between quinones and l-dopa, cyclic voltammetry and UV-Vis spectroscopic study was performed. A sesquiterpenoid hydroquinone, isolated from the marine sponge Dysidea avara (avarol), has been used in this study. The change of apparent heterogeneous rate constant with different avarol/l-dopa ratio indicated that charge transfer could be enhanced at some extent. In addition to this, the obtained results for avarol and hydroquinone (its structural element) were compared. UV-Vis spectroscopic analysis confirmed interaction between l-dopa and avarol or hydroquinone. Taken all together, the interaction of l-dopa was stronger with hydroquinone than with avarol, presumably reflecting the conformational restrains of avarol caused by its terpenoid moiety.",
publisher = "Springer, Dordrecht",
journal = "Reaction Kinetics Mechanisms and Catalysis",
title = "Electrochemical and spectroscopic study of l-dopa interaction with avarol",
pages = "229-219",
number = "1",
volume = "127",
doi = "10.1007/s11144-019-01575-z"
}

Nakarada, D., Pejin, B., Dimic, D., Ivanovic-Sasic, A., Mojović, Z.,& Mojović, M.. (2019). Electrochemical and spectroscopic study of l-dopa interaction with avarol. in Reaction Kinetics Mechanisms and Catalysis
Springer, Dordrecht., 127(1), 219-229.
https://doi.org/10.1007/s11144-019-01575-z

Nakarada D, Pejin B, Dimic D, Ivanovic-Sasic A, Mojović Z, Mojović M. Electrochemical and spectroscopic study of l-dopa interaction with avarol. in Reaction Kinetics Mechanisms and Catalysis. 2019;127(1):219-229.
doi:10.1007/s11144-019-01575-z .

Nakarada, Dura, Pejin, Boris, Dimic, Dusan, Ivanovic-Sasic, Ana, Mojović, Zorica, Mojović, Miloš, "Electrochemical and spectroscopic study of l-dopa interaction with avarol" in Reaction Kinetics Mechanisms and Catalysis, 127, no. 1 (2019):219-229,
https://doi.org/10.1007/s11144-019-01575-z . .

TY  - JOUR
AU  - Pejin, Boris
AU  - Nakarada, Dura
AU  - Novaković, Miroslav
AU  - Tešević, Vele
AU  - Savić, Aleksandar G
AU  - Radotić, Ksenija
AU  - Mojović, Miloš
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/773
AB  - Two volatile samples of the bryozoan Hyalinella punctata (seasonally collected from the same locality) were isolated (hydrodistillation, Clevenger apparatus), identified (GC and GC-MS) and screened in vitro (EPR) for anti-hydroxyl radical activity. The main components of sample 1 (2-ethyl-1-hexanol 37.00%, dodecanol 21.40% and hexanal 8.40%) and sample 2 (2-ethyl-1-hexanol 30.50%, 7-tridecanol 24.60% and 1-hexadecanol 11.80%) were relatively similar. However, more components were present in the sample 2 (17 and 25, respectively). EPR measurements indicated significant anti-hydroxyl radical activity of the both samples (75.00 +/- 6.00% and 87.00 +/- 8.00%, respectively) whereas the generation of other types of free radicals in reaction with hydroxyl radicals was not observed. According to the best of our knowledge, this is the first report of 31 organic compounds from the phylum Bryozoa. Alcohols, aldehydes, ketones, esters and ethers of lower molecular mass appear to be characteristic for the volatiles of these organisms commonly known as moss animals.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - Antioxidant volatiles of the freshwater bryozoan Hyalinella punctata
EP  - 1475
IS  - 18
SP  - 1471
VL  - 28
DO  - 10.1080/14786419.2014.905565
ER  - 

@article{
author = "Pejin, Boris and Nakarada, Dura and Novaković, Miroslav and Tešević, Vele and Savić, Aleksandar G and Radotić, Ksenija and Mojović, Miloš",
year = "2014",
abstract = "Two volatile samples of the bryozoan Hyalinella punctata (seasonally collected from the same locality) were isolated (hydrodistillation, Clevenger apparatus), identified (GC and GC-MS) and screened in vitro (EPR) for anti-hydroxyl radical activity. The main components of sample 1 (2-ethyl-1-hexanol 37.00%, dodecanol 21.40% and hexanal 8.40%) and sample 2 (2-ethyl-1-hexanol 30.50%, 7-tridecanol 24.60% and 1-hexadecanol 11.80%) were relatively similar. However, more components were present in the sample 2 (17 and 25, respectively). EPR measurements indicated significant anti-hydroxyl radical activity of the both samples (75.00 +/- 6.00% and 87.00 +/- 8.00%, respectively) whereas the generation of other types of free radicals in reaction with hydroxyl radicals was not observed. According to the best of our knowledge, this is the first report of 31 organic compounds from the phylum Bryozoa. Alcohols, aldehydes, ketones, esters and ethers of lower molecular mass appear to be characteristic for the volatiles of these organisms commonly known as moss animals.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "Antioxidant volatiles of the freshwater bryozoan Hyalinella punctata",
pages = "1475-1471",
number = "18",
volume = "28",
doi = "10.1080/14786419.2014.905565"
}

Pejin, B., Nakarada, D., Novaković, M., Tešević, V., Savić, A. G., Radotić, K.,& Mojović, M.. (2014). Antioxidant volatiles of the freshwater bryozoan Hyalinella punctata. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 28(18), 1471-1475.
https://doi.org/10.1080/14786419.2014.905565

Pejin B, Nakarada D, Novaković M, Tešević V, Savić AG, Radotić K, Mojović M. Antioxidant volatiles of the freshwater bryozoan Hyalinella punctata. in Natural Product Research. 2014;28(18):1471-1475.
doi:10.1080/14786419.2014.905565 .

Pejin, Boris, Nakarada, Dura, Novaković, Miroslav, Tešević, Vele, Savić, Aleksandar G, Radotić, Ksenija, Mojović, Miloš, "Antioxidant volatiles of the freshwater bryozoan Hyalinella punctata" in Natural Product Research, 28, no. 18 (2014):1471-1475,
https://doi.org/10.1080/14786419.2014.905565 . .