TY  - JOUR
AU  - Marjanović, Žaklina
AU  - Nawaz, Ali
AU  - Stevanović, Katarina
AU  - Saljnikov, Elmira
AU  - Macek, Irena
AU  - Oehl, Fritz
AU  - Wubet, Tesfaye
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1370
AB  - Balkan lowlands bordering with the Pannonia region are inhabited by diverse riparian forests that support production of different truffle species, predominantly the most prized white truffle of Piedmont (Tuber magnatumPico), but also other commercial species (T.macrosporumVitt.,T.aestivumVitt.). Surprisingly, little is known about the native root-associated mycobiome (RAM) of these lowland truffle-producing forests. Therefore, in this study we aim at exploring and comparing the RAMs of three different truffle-producing forests from Kolubara river plane in Serbia. Molecular methods based on next generation sequencing (NGS) were used to evaluate the diversity of root-associated fungal communities and to elucidate the influence of environmental factors on their differentiation. To our knowledge, this is the first study from such habitats with a particular focus on comparative analysis of the RAM in different truffle-producing habitats using a high-throughput sequencing approach. Our results indicated that the alpha diversity of investigated fungal communities was not significantly different between different truffle-producing forests and within a specific forest type, while the seasonal differences in the alpha diversity were only observed in the white truffle-producing forests. Taxonomic profiling at phylum level indicated the dominance of fungal OTUs belonging to phylum Ascomycota and Basidiomycota, with very minor presence of other phyla. Distinct community structures of root-associated mycobiomes were observed for white, mixed, and black truffle-producing forests. The core mycobiome analysis indicated a fair share of fungal genera present exclusively in white and black truffle-producing forest, while the core genera of mixed truffle-producing forests were shared with both white and black truffle-producing forests. The majority of detected fungal OTUs in all three forest types were symbiotrophs, with ectomycorrhizal fungi being a dominant functional guild. Apart from assumed vegetation factor, differentiation of fungal communities was driven by factors connected to the distance from the river and exposure to fluvial activities, soil age, structure, and pH. Overall, Pannonian riparian forests appear to host diverse root-associated fungal communities that are strongly shaped by variation in soil conditions.
PB  - MDPI, Basel
T2  - Microorganisms
T1  - Root-Associated Mycobiome Differentiate between Habitats Supporting Production of Different Truffle Species in Serbian Riparian Forests
IS  - 9
VL  - 8
DO  - 10.3390/microorganisms8091331
ER  - 

@article{
author = "Marjanović, Žaklina and Nawaz, Ali and Stevanović, Katarina and Saljnikov, Elmira and Macek, Irena and Oehl, Fritz and Wubet, Tesfaye",
year = "2020",
abstract = "Balkan lowlands bordering with the Pannonia region are inhabited by diverse riparian forests that support production of different truffle species, predominantly the most prized white truffle of Piedmont (Tuber magnatumPico), but also other commercial species (T.macrosporumVitt.,T.aestivumVitt.). Surprisingly, little is known about the native root-associated mycobiome (RAM) of these lowland truffle-producing forests. Therefore, in this study we aim at exploring and comparing the RAMs of three different truffle-producing forests from Kolubara river plane in Serbia. Molecular methods based on next generation sequencing (NGS) were used to evaluate the diversity of root-associated fungal communities and to elucidate the influence of environmental factors on their differentiation. To our knowledge, this is the first study from such habitats with a particular focus on comparative analysis of the RAM in different truffle-producing habitats using a high-throughput sequencing approach. Our results indicated that the alpha diversity of investigated fungal communities was not significantly different between different truffle-producing forests and within a specific forest type, while the seasonal differences in the alpha diversity were only observed in the white truffle-producing forests. Taxonomic profiling at phylum level indicated the dominance of fungal OTUs belonging to phylum Ascomycota and Basidiomycota, with very minor presence of other phyla. Distinct community structures of root-associated mycobiomes were observed for white, mixed, and black truffle-producing forests. The core mycobiome analysis indicated a fair share of fungal genera present exclusively in white and black truffle-producing forest, while the core genera of mixed truffle-producing forests were shared with both white and black truffle-producing forests. The majority of detected fungal OTUs in all three forest types were symbiotrophs, with ectomycorrhizal fungi being a dominant functional guild. Apart from assumed vegetation factor, differentiation of fungal communities was driven by factors connected to the distance from the river and exposure to fluvial activities, soil age, structure, and pH. Overall, Pannonian riparian forests appear to host diverse root-associated fungal communities that are strongly shaped by variation in soil conditions.",
publisher = "MDPI, Basel",
journal = "Microorganisms",
title = "Root-Associated Mycobiome Differentiate between Habitats Supporting Production of Different Truffle Species in Serbian Riparian Forests",
number = "9",
volume = "8",
doi = "10.3390/microorganisms8091331"
}

Marjanović, Ž., Nawaz, A., Stevanović, K., Saljnikov, E., Macek, I., Oehl, F.,& Wubet, T.. (2020). Root-Associated Mycobiome Differentiate between Habitats Supporting Production of Different Truffle Species in Serbian Riparian Forests. in Microorganisms
MDPI, Basel., 8(9).
https://doi.org/10.3390/microorganisms8091331

Marjanović Ž, Nawaz A, Stevanović K, Saljnikov E, Macek I, Oehl F, Wubet T. Root-Associated Mycobiome Differentiate between Habitats Supporting Production of Different Truffle Species in Serbian Riparian Forests. in Microorganisms. 2020;8(9).
doi:10.3390/microorganisms8091331 .

Marjanović, Žaklina, Nawaz, Ali, Stevanović, Katarina, Saljnikov, Elmira, Macek, Irena, Oehl, Fritz, Wubet, Tesfaye, "Root-Associated Mycobiome Differentiate between Habitats Supporting Production of Different Truffle Species in Serbian Riparian Forests" in Microorganisms, 8, no. 9 (2020),
https://doi.org/10.3390/microorganisms8091331 . .

TY  - JOUR
AU  - Karaman, Maja
AU  - Atlagic, Kristina
AU  - Novaković, Aleksandra
AU  - Sibul, Filip
AU  - Zivić, Miroslav
AU  - Stevanović, Katarina
AU  - Pejin, Boris
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1198
AB  - Compared to plants, nowadays mushrooms attract more attention as functional foods, due to a number of advantages in manipulating them. This study aimed to screen the chemical composition (fatty acids and phenolics) and antioxidant potential (OH center dot, 2,2-diphenyl-1-picrylhydrazyl (DPPH center dot) and ferric reducing ability of plasma (FRAP)) of two edible mushrooms, Coprinus comatus and Coprinellus truncorum, collected from nature and submerged cultivation. Partial least square regression analysis has pointed out the importance of some fatty acids-more precisely, unsaturated fatty acids (UFAs) followed by fatty acids possessing both short (C6:0 and C8:0) and long (C23:0 and C24:0) saturated chains-and phenolic compounds (such as protocatechuic acid, daidzein, p-hydroxybenzoic acid, genistein and vanillic acid) for promising anti-OH center dot, FRAP and anti-DPPH center dot activities, respectively. However, other fatty acids (C16:0, C18:0 and C18:3n3) along with the flavonol isorhamnetin are actually suspected to negatively affect (by acting pro-oxidative) the aforementioned parameters, respectively. Taken together, design of new food supplements targeting oxidative stress might be predominantly based on the various UFAs combinations (C18:2n6, C20:1, C20:2, C20:4n6, C22:2, C22:1n9, etc.), particularly if OH center dot is suspected to play an important role.
PB  - MDPI, Basel
T2  - Antioxidants
T1  - Fatty Acids Predominantly Affect Anti-Hydroxyl Radical Activity and FRAP Value: The Case Study of Two Edible Mushrooms
IS  - 10
VL  - 8
DO  - 10.3390/antiox8100480
ER  - 

@article{
author = "Karaman, Maja and Atlagic, Kristina and Novaković, Aleksandra and Sibul, Filip and Zivić, Miroslav and Stevanović, Katarina and Pejin, Boris",
year = "2019",
abstract = "Compared to plants, nowadays mushrooms attract more attention as functional foods, due to a number of advantages in manipulating them. This study aimed to screen the chemical composition (fatty acids and phenolics) and antioxidant potential (OH center dot, 2,2-diphenyl-1-picrylhydrazyl (DPPH center dot) and ferric reducing ability of plasma (FRAP)) of two edible mushrooms, Coprinus comatus and Coprinellus truncorum, collected from nature and submerged cultivation. Partial least square regression analysis has pointed out the importance of some fatty acids-more precisely, unsaturated fatty acids (UFAs) followed by fatty acids possessing both short (C6:0 and C8:0) and long (C23:0 and C24:0) saturated chains-and phenolic compounds (such as protocatechuic acid, daidzein, p-hydroxybenzoic acid, genistein and vanillic acid) for promising anti-OH center dot, FRAP and anti-DPPH center dot activities, respectively. However, other fatty acids (C16:0, C18:0 and C18:3n3) along with the flavonol isorhamnetin are actually suspected to negatively affect (by acting pro-oxidative) the aforementioned parameters, respectively. Taken together, design of new food supplements targeting oxidative stress might be predominantly based on the various UFAs combinations (C18:2n6, C20:1, C20:2, C20:4n6, C22:2, C22:1n9, etc.), particularly if OH center dot is suspected to play an important role.",
publisher = "MDPI, Basel",
journal = "Antioxidants",
title = "Fatty Acids Predominantly Affect Anti-Hydroxyl Radical Activity and FRAP Value: The Case Study of Two Edible Mushrooms",
number = "10",
volume = "8",
doi = "10.3390/antiox8100480"
}

Karaman, M., Atlagic, K., Novaković, A., Sibul, F., Zivić, M., Stevanović, K.,& Pejin, B.. (2019). Fatty Acids Predominantly Affect Anti-Hydroxyl Radical Activity and FRAP Value: The Case Study of Two Edible Mushrooms. in Antioxidants
MDPI, Basel., 8(10).
https://doi.org/10.3390/antiox8100480

Karaman M, Atlagic K, Novaković A, Sibul F, Zivić M, Stevanović K, Pejin B. Fatty Acids Predominantly Affect Anti-Hydroxyl Radical Activity and FRAP Value: The Case Study of Two Edible Mushrooms. in Antioxidants. 2019;8(10).
doi:10.3390/antiox8100480 .

Karaman, Maja, Atlagic, Kristina, Novaković, Aleksandra, Sibul, Filip, Zivić, Miroslav, Stevanović, Katarina, Pejin, Boris, "Fatty Acids Predominantly Affect Anti-Hydroxyl Radical Activity and FRAP Value: The Case Study of Two Edible Mushrooms" in Antioxidants, 8, no. 10 (2019),
https://doi.org/10.3390/antiox8100480 . .