TY  - CONF
AU  - Miletić, Katarina
AU  - Mošić, Miloš
AU  - Milutinović, Marijana
AU  - Šušić, Nikola
AU  - Kasalica, Vidoje
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2053
AB  - In vivo measurements of the optical properties of plant leaves, by spectrophotometric
methods, could potentially provide very useful information considering plant's health. This
research is focused on developing nondestructive optical processing method to obtain useful
information for overall plant health evaluation. The analyses of the leaf spectroscopy (in the
broad wavelength range) is well present in majority of the literature. The absorption spectra
of chloroplast pigments provide clues to the relative effectiveness of different wavelengths
for driving photosynthesis, since light can perform work in chloroplasts only if it is absorbed.
This experimental procedure describes a novel experimental setup that enables continuous
measurements of the optical reflection and transmission coefficients of broad-leaved plants.
For each of 20 channels, the source of light is a red Signal LED with the spectral emission
maximum at 665 nm. Special attention is given to the development of data collection
software, as well as procedures for calibration of the measuring processing system and
handmade methyl methacrylate leaf holders. Monitoring the evolution of the plant activity in
real-time has resulted in the graph of the spectral Circadian rhythm as a function of time.
Signatures from spectroscopic optical imaging could be successfully used to track nutritional
disorders before visual symptoms are observed. The setup was tested on: Ocimum basilicum
L. (the plants were grown in the hydroponics); Phaseolus vulgaris L., Zea mays L. (seeds
were germinated in commercial humus), Guzmania lingulata (L.) Mez, Vriesea carinata
Wawra, variegated geranium (L.) L'Hér. etc. The condition of the plants under test was
assessed by the more common (destructive) methods such as: measurements of the
determination of the photosynthetic pigment content, dry weight determination and the
efficiency of PSII. Several biological parameters were observed, calculated and compared to
the graphs of optical transmission dependence in real time. Currently, we are focused to
update knowledge about fast and subtle changes in chloroplast movements during dark-light-
dark transition and to relate different location of chloroplast to their photosynthetic capacity.
PB  - Institute of Technical Sciences of SASA
C3  - Twentieth Young Researchers’ Conference Materials Science and Engineering
T1  - Innovative nondestructive optical method for plant overall health evaluation
EP  - 52
SP  - 52
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2053
ER  - 

@conference{
author = "Miletić, Katarina and Mošić, Miloš and Milutinović, Marijana and Šušić, Nikola and Kasalica, Vidoje",
year = "2022",
abstract = "In vivo measurements of the optical properties of plant leaves, by spectrophotometric
methods, could potentially provide very useful information considering plant's health. This
research is focused on developing nondestructive optical processing method to obtain useful
information for overall plant health evaluation. The analyses of the leaf spectroscopy (in the
broad wavelength range) is well present in majority of the literature. The absorption spectra
of chloroplast pigments provide clues to the relative effectiveness of different wavelengths
for driving photosynthesis, since light can perform work in chloroplasts only if it is absorbed.
This experimental procedure describes a novel experimental setup that enables continuous
measurements of the optical reflection and transmission coefficients of broad-leaved plants.
For each of 20 channels, the source of light is a red Signal LED with the spectral emission
maximum at 665 nm. Special attention is given to the development of data collection
software, as well as procedures for calibration of the measuring processing system and
handmade methyl methacrylate leaf holders. Monitoring the evolution of the plant activity in
real-time has resulted in the graph of the spectral Circadian rhythm as a function of time.
Signatures from spectroscopic optical imaging could be successfully used to track nutritional
disorders before visual symptoms are observed. The setup was tested on: Ocimum basilicum
L. (the plants were grown in the hydroponics); Phaseolus vulgaris L., Zea mays L. (seeds
were germinated in commercial humus), Guzmania lingulata (L.) Mez, Vriesea carinata
Wawra, variegated geranium (L.) L'Hér. etc. The condition of the plants under test was
assessed by the more common (destructive) methods such as: measurements of the
determination of the photosynthetic pigment content, dry weight determination and the
efficiency of PSII. Several biological parameters were observed, calculated and compared to
the graphs of optical transmission dependence in real time. Currently, we are focused to
update knowledge about fast and subtle changes in chloroplast movements during dark-light-
dark transition and to relate different location of chloroplast to their photosynthetic capacity.",
publisher = "Institute of Technical Sciences of SASA",
journal = "Twentieth Young Researchers’ Conference Materials Science and Engineering",
title = "Innovative nondestructive optical method for plant overall health evaluation",
pages = "52-52",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2053"
}

Miletić, K., Mošić, M., Milutinović, M., Šušić, N.,& Kasalica, V.. (2022). Innovative nondestructive optical method for plant overall health evaluation. in Twentieth Young Researchers’ Conference Materials Science and Engineering
Institute of Technical Sciences of SASA., 52-52.
https://hdl.handle.net/21.15107/rcub_rimsi_2053

Miletić K, Mošić M, Milutinović M, Šušić N, Kasalica V. Innovative nondestructive optical method for plant overall health evaluation. in Twentieth Young Researchers’ Conference Materials Science and Engineering. 2022;:52-52.
https://hdl.handle.net/21.15107/rcub_rimsi_2053 .

Miletić, Katarina, Mošić, Miloš, Milutinović, Marijana, Šušić, Nikola, Kasalica, Vidoje, "Innovative nondestructive optical method for plant overall health evaluation" in Twentieth Young Researchers’ Conference Materials Science and Engineering (2022):52-52,
https://hdl.handle.net/21.15107/rcub_rimsi_2053 .