TY  - JOUR
AU  - Mojović, Miloš
AU  - Spasojević, Ivan
AU  - Vuletic, M
AU  - Vučinić, Željko
AU  - Bačić, Goran
PY  - 2005
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/142
AB  - Plant plasma membranes are known to produce superoxide radicals, while the production of hydroxyl radical is thought to occur only in the cell wall. In this work it was demonstrated using combined spin-trap and spin-probe EPR spectroscopic techniques, that plant plasma membranes do produce superoxide and hydroxyl radicals but by kinetically different mechanisms. The results show that superoxide and hydroxyl radicals can be detected by DMPO spin-trap and that the mechanisms and location of their production call be differentiated using the reduction of spin-probes Tempone and 7-DS. It was shown that the mechanism of production of oxygen reactive species is NADH dependent and diphenylene iodonium inhibited. The kinetics of the reduction of Temponc, combined with scavengers or the absence of NADH indicates that hydroxyl radicals are produced by a mechanism independent of that of superoxide production. It was shown that a combination of the spill-probe and spin-trap technique can be used in free radical studies of biological systems, with a number of advantages inherent to them.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes
EP  - 186
IS  - 2
SP  - 177
VL  - 70
DO  - 10.2298/JSC0502177M
ER  - 

@article{
author = "Mojović, Miloš and Spasojević, Ivan and Vuletic, M and Vučinić, Željko and Bačić, Goran",
year = "2005",
abstract = "Plant plasma membranes are known to produce superoxide radicals, while the production of hydroxyl radical is thought to occur only in the cell wall. In this work it was demonstrated using combined spin-trap and spin-probe EPR spectroscopic techniques, that plant plasma membranes do produce superoxide and hydroxyl radicals but by kinetically different mechanisms. The results show that superoxide and hydroxyl radicals can be detected by DMPO spin-trap and that the mechanisms and location of their production call be differentiated using the reduction of spin-probes Tempone and 7-DS. It was shown that the mechanism of production of oxygen reactive species is NADH dependent and diphenylene iodonium inhibited. The kinetics of the reduction of Temponc, combined with scavengers or the absence of NADH indicates that hydroxyl radicals are produced by a mechanism independent of that of superoxide production. It was shown that a combination of the spill-probe and spin-trap technique can be used in free radical studies of biological systems, with a number of advantages inherent to them.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes",
pages = "186-177",
number = "2",
volume = "70",
doi = "10.2298/JSC0502177M"
}

Mojović, M., Spasojević, I., Vuletic, M., Vučinić, Ž.,& Bačić, G.. (2005). An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 70(2), 177-186.
https://doi.org/10.2298/JSC0502177M

Mojović M, Spasojević I, Vuletic M, Vučinić Ž, Bačić G. An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes. in Journal of the Serbian Chemical Society. 2005;70(2):177-186.
doi:10.2298/JSC0502177M .

Mojović, Miloš, Spasojević, Ivan, Vuletic, M, Vučinić, Željko, Bačić, Goran, "An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes" in Journal of the Serbian Chemical Society, 70, no. 2 (2005):177-186,
https://doi.org/10.2298/JSC0502177M . .

TY  - JOUR
AU  - Vuletic, M
AU  - Radotić, Ksenija
AU  - Vučinić, Željko
PY  - 1998
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/39
AB  - The identity of the naturally occurring compounds that accept electrons from plasma membrane-bound redox systems in vivo is obscure. We analysed the effect of ascorbate, oxygen, iron, as well as their free radical forms, and also the free radical-generating and -quenching systems on the trans-root electrical potential, which had previously been shown to be coupled to plasma membrane-bound redox systems. The material was the primary root of 8-day-old maize (Zea mays L.) seedlings. Trans-root electrical potential difference was measured across excised roots. Different ascorbate (ascorbate, dehydroascorbate and ascorbate free radical) and oxygen redox forms (superoxide and hydroxide radicals and hydrogen peroxide), as well as scavenging agents of oxygen species (superoxide dismutase, catalase, mannitol), and ferric and ferrous ions were added to the solution flowing around the root. Ascorbate free radical induced the greatest depolarization of the trans-root potential when compared to other ascorbate redox forms, which is consistent with its suggested role as a natural electron acceptor. Addition of xanthine oxidase, with or without xanthine, also produced depolarizing effects. The presence of SOD magnified this effect both with ascorbate free radical and xanthine oxidase. When ferric or ferrous chloride and ferric EDTA were applied to the bathing medium, only free ferric ion produced a very pronounced depolarization. The magnitude and kinetics of trans-root potential depolarization, induced by the ascorbate redox forms and systems for the generation and scavenging of oxygen species, argue in favour of the mutually competing electron transfer role of ascorbate free radicals and superoxide radicals in the extracellular space of the root. These results provide evidence that at least a part of the electrical potential difference occurring across plant roots arises from current flow from the symplast, via the plasma membrane-bound redox systems, to naturally occurring compounds in the apoplast, and that this transfer is achieved through the mediation of their free radical forms.
PB  - Wiley, Hoboken
T2  - Physiologia Plantarum
T1  - Free radical involvement in the generation of trans-root potential
EP  - 782
IS  - 4
SP  - 777
VL  - 104
DO  - 10.1034/j.1399-3054.1998.1040438.x
ER  - 

@article{
author = "Vuletic, M and Radotić, Ksenija and Vučinić, Željko",
year = "1998",
abstract = "The identity of the naturally occurring compounds that accept electrons from plasma membrane-bound redox systems in vivo is obscure. We analysed the effect of ascorbate, oxygen, iron, as well as their free radical forms, and also the free radical-generating and -quenching systems on the trans-root electrical potential, which had previously been shown to be coupled to plasma membrane-bound redox systems. The material was the primary root of 8-day-old maize (Zea mays L.) seedlings. Trans-root electrical potential difference was measured across excised roots. Different ascorbate (ascorbate, dehydroascorbate and ascorbate free radical) and oxygen redox forms (superoxide and hydroxide radicals and hydrogen peroxide), as well as scavenging agents of oxygen species (superoxide dismutase, catalase, mannitol), and ferric and ferrous ions were added to the solution flowing around the root. Ascorbate free radical induced the greatest depolarization of the trans-root potential when compared to other ascorbate redox forms, which is consistent with its suggested role as a natural electron acceptor. Addition of xanthine oxidase, with or without xanthine, also produced depolarizing effects. The presence of SOD magnified this effect both with ascorbate free radical and xanthine oxidase. When ferric or ferrous chloride and ferric EDTA were applied to the bathing medium, only free ferric ion produced a very pronounced depolarization. The magnitude and kinetics of trans-root potential depolarization, induced by the ascorbate redox forms and systems for the generation and scavenging of oxygen species, argue in favour of the mutually competing electron transfer role of ascorbate free radicals and superoxide radicals in the extracellular space of the root. These results provide evidence that at least a part of the electrical potential difference occurring across plant roots arises from current flow from the symplast, via the plasma membrane-bound redox systems, to naturally occurring compounds in the apoplast, and that this transfer is achieved through the mediation of their free radical forms.",
publisher = "Wiley, Hoboken",
journal = "Physiologia Plantarum",
title = "Free radical involvement in the generation of trans-root potential",
pages = "782-777",
number = "4",
volume = "104",
doi = "10.1034/j.1399-3054.1998.1040438.x"
}

Vuletic, M., Radotić, K.,& Vučinić, Ž.. (1998). Free radical involvement in the generation of trans-root potential. in Physiologia Plantarum
Wiley, Hoboken., 104(4), 777-782.
https://doi.org/10.1034/j.1399-3054.1998.1040438.x

Vuletic M, Radotić K, Vučinić Ž. Free radical involvement in the generation of trans-root potential. in Physiologia Plantarum. 1998;104(4):777-782.
doi:10.1034/j.1399-3054.1998.1040438.x .

Vuletic, M, Radotić, Ksenija, Vučinić, Željko, "Free radical involvement in the generation of trans-root potential" in Physiologia Plantarum, 104, no. 4 (1998):777-782,
https://doi.org/10.1034/j.1399-3054.1998.1040438.x . .