TY  - JOUR
AU  - Martinović, Rajko
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Jokanović, Sandra
AU  - Gačić, Zoran
AU  - Joksimović, Danijela
AU  - Durović, Mirko
AU  - Kljajic, Zoran
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1009
AB  - This study gives an insight in sensitivity of heart rate (Hr) of Mytilus galloprovincialis as a physiological biomarker. Impact of tributyltin chloride (TBT-Cl) on Hr was studied in parallel with evaluation of mutagenic, genotoxic and cytotoxic potential of TBT-Cl (10,100 and 1000 mu g/L) within 96 h treatment in static conditions. Mutagenic potential was assessed by SOS/umuC assay while genotoxicity was assessed in haemocytes of M. galloprovincialis by using the comet assay and the micronucleus test. Benzo(a)pyrene (B(a)P) was used as a positive control. Hr variations detected in TBT-Cl treatments can be linked to data obtained in the genotoxicological assays indicating that Hr can be considered and used as a reliable physiological biomarker for detecting the presence of organotin compounds. However despite the observed genotoxic potential of B(a)P, a noteworthy Hr response was not observed which further questions the potential of Hr in the detection of different types of pollutants.
PB  - Elsevier Science Bv, Amsterdam
T2  - Environmental Toxicology and Pharmacology
T1  - Comparative assessment of cardiac activity and DNA damage in haemocytes of the Mediterranean mussel Mytilus galloprovincialis in exposure to tributyltin chloride
EP  - 174
SP  - 165
VL  - 47
DO  - 10.1016/j.etap.2016.09.019
ER  - 

@article{
author = "Martinović, Rajko and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Jokanović, Sandra and Gačić, Zoran and Joksimović, Danijela and Durović, Mirko and Kljajic, Zoran and Vukovic-Gacic, Branka",
year = "2016",
abstract = "This study gives an insight in sensitivity of heart rate (Hr) of Mytilus galloprovincialis as a physiological biomarker. Impact of tributyltin chloride (TBT-Cl) on Hr was studied in parallel with evaluation of mutagenic, genotoxic and cytotoxic potential of TBT-Cl (10,100 and 1000 mu g/L) within 96 h treatment in static conditions. Mutagenic potential was assessed by SOS/umuC assay while genotoxicity was assessed in haemocytes of M. galloprovincialis by using the comet assay and the micronucleus test. Benzo(a)pyrene (B(a)P) was used as a positive control. Hr variations detected in TBT-Cl treatments can be linked to data obtained in the genotoxicological assays indicating that Hr can be considered and used as a reliable physiological biomarker for detecting the presence of organotin compounds. However despite the observed genotoxic potential of B(a)P, a noteworthy Hr response was not observed which further questions the potential of Hr in the detection of different types of pollutants.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Environmental Toxicology and Pharmacology",
title = "Comparative assessment of cardiac activity and DNA damage in haemocytes of the Mediterranean mussel Mytilus galloprovincialis in exposure to tributyltin chloride",
pages = "174-165",
volume = "47",
doi = "10.1016/j.etap.2016.09.019"
}

Martinović, R., Kolarević, S., Kracun-Kolarević, M., Kostić-Vuković, J., Jokanović, S., Gačić, Z., Joksimović, D., Durović, M., Kljajic, Z.,& Vukovic-Gacic, B.. (2016). Comparative assessment of cardiac activity and DNA damage in haemocytes of the Mediterranean mussel Mytilus galloprovincialis in exposure to tributyltin chloride. in Environmental Toxicology and Pharmacology
Elsevier Science Bv, Amsterdam., 47, 165-174.
https://doi.org/10.1016/j.etap.2016.09.019

Martinović R, Kolarević S, Kracun-Kolarević M, Kostić-Vuković J, Jokanović S, Gačić Z, Joksimović D, Durović M, Kljajic Z, Vukovic-Gacic B. Comparative assessment of cardiac activity and DNA damage in haemocytes of the Mediterranean mussel Mytilus galloprovincialis in exposure to tributyltin chloride. in Environmental Toxicology and Pharmacology. 2016;47:165-174.
doi:10.1016/j.etap.2016.09.019 .

Martinović, Rajko, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Jokanović, Sandra, Gačić, Zoran, Joksimović, Danijela, Durović, Mirko, Kljajic, Zoran, Vukovic-Gacic, Branka, "Comparative assessment of cardiac activity and DNA damage in haemocytes of the Mediterranean mussel Mytilus galloprovincialis in exposure to tributyltin chloride" in Environmental Toxicology and Pharmacology, 47 (2016):165-174,
https://doi.org/10.1016/j.etap.2016.09.019 . .

TY  - JOUR
AU  - Martinović, Rajko
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Marković, Sandra
AU  - Gačić, Zoran
AU  - Kljajic, Zoran
AU  - Vukovic-Gacic, Branka
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/857
AB  - The effects of ex situ exposure of Mytilus galloprovincialis to Superdispersant-25 (S-25), diesel oil and dispersed diesel oil mixtures were studied by the impact on level of DNA damage in haemocytes (comet assay) and the cardiac activity patterns of mussels. Specimens were exposed for 72 h in a static system to diesel oil (100 mu L/L and 1 mL/L), S-25 (5 and 50 mu L/L), and dispersed diesel oil mixtures M1 (diesel oil 100 g mu L/L + S-25 5 mu L/L) and M2 (diesel oil 1 mL/L + S-25 50 mu L/L). For positive control 40 mu M CdCl2 was used. The comet assay results indicated genotoxic potential of S-25 while the effects of diesel oil alone were not observed. The highest response was detected for M1 while the effects of M2 were not detected. The heart rate disorders were recorded for the diesel oil (1 mL/L), S-25 (50 mu L/L) and both dispersed diesel oil mixtures.
PB  - Elsevier Sci Ltd, Oxford
T2  - Marine Environmental Research
T1  - Genotoxic potential and heart rate disorders in the Mediterranean mussel Mytilus galloprovincialis exposed to Superdispersant-25 and dispersed diesel oil
EP  - 90
SP  - 83
VL  - 108
DO  - 10.1016/j.marenvres.2015.05.001
ER  - 

@article{
author = "Martinović, Rajko and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Marković, Sandra and Gačić, Zoran and Kljajic, Zoran and Vukovic-Gacic, Branka",
year = "2015",
abstract = "The effects of ex situ exposure of Mytilus galloprovincialis to Superdispersant-25 (S-25), diesel oil and dispersed diesel oil mixtures were studied by the impact on level of DNA damage in haemocytes (comet assay) and the cardiac activity patterns of mussels. Specimens were exposed for 72 h in a static system to diesel oil (100 mu L/L and 1 mL/L), S-25 (5 and 50 mu L/L), and dispersed diesel oil mixtures M1 (diesel oil 100 g mu L/L + S-25 5 mu L/L) and M2 (diesel oil 1 mL/L + S-25 50 mu L/L). For positive control 40 mu M CdCl2 was used. The comet assay results indicated genotoxic potential of S-25 while the effects of diesel oil alone were not observed. The highest response was detected for M1 while the effects of M2 were not detected. The heart rate disorders were recorded for the diesel oil (1 mL/L), S-25 (50 mu L/L) and both dispersed diesel oil mixtures.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Marine Environmental Research",
title = "Genotoxic potential and heart rate disorders in the Mediterranean mussel Mytilus galloprovincialis exposed to Superdispersant-25 and dispersed diesel oil",
pages = "90-83",
volume = "108",
doi = "10.1016/j.marenvres.2015.05.001"
}

Martinović, R., Kolarević, S., Kracun-Kolarević, M., Kostić-Vuković, J., Marković, S., Gačić, Z., Kljajic, Z.,& Vukovic-Gacic, B.. (2015). Genotoxic potential and heart rate disorders in the Mediterranean mussel Mytilus galloprovincialis exposed to Superdispersant-25 and dispersed diesel oil. in Marine Environmental Research
Elsevier Sci Ltd, Oxford., 108, 83-90.
https://doi.org/10.1016/j.marenvres.2015.05.001

Martinović R, Kolarević S, Kracun-Kolarević M, Kostić-Vuković J, Marković S, Gačić Z, Kljajic Z, Vukovic-Gacic B. Genotoxic potential and heart rate disorders in the Mediterranean mussel Mytilus galloprovincialis exposed to Superdispersant-25 and dispersed diesel oil. in Marine Environmental Research. 2015;108:83-90.
doi:10.1016/j.marenvres.2015.05.001 .

Martinović, Rajko, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Marković, Sandra, Gačić, Zoran, Kljajic, Zoran, Vukovic-Gacic, Branka, "Genotoxic potential and heart rate disorders in the Mediterranean mussel Mytilus galloprovincialis exposed to Superdispersant-25 and dispersed diesel oil" in Marine Environmental Research, 108 (2015):83-90,
https://doi.org/10.1016/j.marenvres.2015.05.001 . .

TY  - JOUR
AU  - Heberger, Karoly
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Sunjog, Karolina
AU  - Gačić, Zoran
AU  - Kljajic, Zoran
AU  - Mitric, Milena
AU  - Vukovic-Gacic, Branka
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/819
AB  - Specimens of the mussel Mytilus galloprovincialis were collected from five sites in the Boka Kotorska Bay (Adriatic Sea, Montenegro) during the period summer 2011-autumn 2012. Three types of tissue, haemolymph, digestive gland were used for assessment of DNA damage. Images of randomly selected cells were analyzed with a fluorescence microscope and image analysis by the Comet Assay IV Image-analysis system. Three parameters, viz, tail length, tail intensity and Olive tail moment were analyzed on 4200 nuclei per cell type. We observed variations in the level of DNA damage in mussels collected at different sites, as well as seasonal variations in response. Sum of ranking differences (SRD) was implemented to compare use of different types of cell and different measure of comet tail per nucleus. Numerical scales were transferred into ranks, range scaling between 0 and 1; standardization and normalization were carried out. SRD selected the best (and worst) combinations: tail moment is the best for all data treatment and for all organs; second best is tail length, and intensity ranks third (except for digestive gland). The differences were significant at the 5% level. Whereas gills and haemolymph cells do not differ significantly, cells of the digestive gland are much more suitable to estimate genotoxicity. Variance analysis decomposed the effect of different factors on the SRD values. This unique combination has provided not only the relative importance of factors, but also an overall evaluation: the best evaluation method, the best data pre-treatment, etc., were chosen even for partially contradictory data. The rank transformation is superior to any other way of scaling, which is proven by ordering the SRD values by SRD again, and by cross validation.
PB  - Elsevier, Amsterdam
T2  - Mutation Research-Genetic Toxicology and Environmental Mutagenesis
T1  - Evaluation of single-cell gel electrophoresis data: Combination of variance analysis with sum of ranking differences
EP  - 22
SP  - 15
VL  - 771
DO  - 10.1016/j.mrgentox.2014.04.028
ER  - 

@article{
author = "Heberger, Karoly and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Sunjog, Karolina and Gačić, Zoran and Kljajic, Zoran and Mitric, Milena and Vukovic-Gacic, Branka",
year = "2014",
abstract = "Specimens of the mussel Mytilus galloprovincialis were collected from five sites in the Boka Kotorska Bay (Adriatic Sea, Montenegro) during the period summer 2011-autumn 2012. Three types of tissue, haemolymph, digestive gland were used for assessment of DNA damage. Images of randomly selected cells were analyzed with a fluorescence microscope and image analysis by the Comet Assay IV Image-analysis system. Three parameters, viz, tail length, tail intensity and Olive tail moment were analyzed on 4200 nuclei per cell type. We observed variations in the level of DNA damage in mussels collected at different sites, as well as seasonal variations in response. Sum of ranking differences (SRD) was implemented to compare use of different types of cell and different measure of comet tail per nucleus. Numerical scales were transferred into ranks, range scaling between 0 and 1; standardization and normalization were carried out. SRD selected the best (and worst) combinations: tail moment is the best for all data treatment and for all organs; second best is tail length, and intensity ranks third (except for digestive gland). The differences were significant at the 5% level. Whereas gills and haemolymph cells do not differ significantly, cells of the digestive gland are much more suitable to estimate genotoxicity. Variance analysis decomposed the effect of different factors on the SRD values. This unique combination has provided not only the relative importance of factors, but also an overall evaluation: the best evaluation method, the best data pre-treatment, etc., were chosen even for partially contradictory data. The rank transformation is superior to any other way of scaling, which is proven by ordering the SRD values by SRD again, and by cross validation.",
publisher = "Elsevier, Amsterdam",
journal = "Mutation Research-Genetic Toxicology and Environmental Mutagenesis",
title = "Evaluation of single-cell gel electrophoresis data: Combination of variance analysis with sum of ranking differences",
pages = "22-15",
volume = "771",
doi = "10.1016/j.mrgentox.2014.04.028"
}

Heberger, K., Kolarević, S., Kracun-Kolarević, M., Sunjog, K., Gačić, Z., Kljajic, Z., Mitric, M.,& Vukovic-Gacic, B.. (2014). Evaluation of single-cell gel electrophoresis data: Combination of variance analysis with sum of ranking differences. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis
Elsevier, Amsterdam., 771, 15-22.
https://doi.org/10.1016/j.mrgentox.2014.04.028

Heberger K, Kolarević S, Kracun-Kolarević M, Sunjog K, Gačić Z, Kljajic Z, Mitric M, Vukovic-Gacic B. Evaluation of single-cell gel electrophoresis data: Combination of variance analysis with sum of ranking differences. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis. 2014;771:15-22.
doi:10.1016/j.mrgentox.2014.04.028 .

Heberger, Karoly, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Sunjog, Karolina, Gačić, Zoran, Kljajic, Zoran, Mitric, Milena, Vukovic-Gacic, Branka, "Evaluation of single-cell gel electrophoresis data: Combination of variance analysis with sum of ranking differences" in Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 771 (2014):15-22,
https://doi.org/10.1016/j.mrgentox.2014.04.028 . .