TY  - JOUR
AU  - Djalović, Ivica
AU  - Mitrović, Petar
AU  - Trivan, Goran
AU  - Jelušić, Aleksandra
AU  - Pezo, Lato
AU  - Janić Hajnal, Elizabet
AU  - Popović Milovanović, Tatjana
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3208
AB  - Infections with phytoplasma present one of the most significant biotic stresses influencing
plant health, growth, and production. The phytoplasma ‘Candidatus Phytoplasma solani’ infects
a variety of plant species. This pathogen impacts the physiological and morphological characteristics of plants causing stunting, yellowing, leaf curling, and other symptoms that can lead to significant economic losses. The aim of this study was to determine biochemical changes in peony (Paeonia tenuifolia L.), mint (Mentha × piperita L.), and dill (Anethum graveolens L.) induced by ‘Ca. Phytoplasma solani’ in Serbia as well as to predict the impact of the biotic stress using artificial neural network (ANN) modeling. The phylogenetic position of the Serbian ‘Ca. Phytoplasma solani’ strains originated from the tested hosts using 16S rRNA (peony and carrot strains) and plsC (mint and dill strains) sequences indicated by their genetic homogeneity despite the host of origin. Biochemical parameters significantly differed in asymptomatic and symptomatic plants, except for total anthocyanidins contents in dill and the capacity of peony and mint extracts to neutralize superoxide anions and hydroxyl radicals, respectively. Principal Component Analysis (PCA) showed a correlation between different chemical parameters and revealed a clear separation among the samples. Based on the ANN performance, the optimal number of hidden neurons for the calculation of TS, RG, PAL, LP, NBT, •OH, TP, TT, Tflav, Tpro, Tant, DPPH, and Car was nine (using MLP 8-9-13), as it produced high r2 values (1.000 during the training period) and low SOS values. Developing an effective early warning system for the detection of plant diseases in different plant species is critical for improving crop yield and quality.
PB  - MDPI
T2  - Horticulturae
T1  - The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach
IS  - 5
SP  - 426
VL  - 10
DO  - 10.3390/horticulturae10050426
ER  - 

@article{
author = "Djalović, Ivica and Mitrović, Petar and Trivan, Goran and Jelušić, Aleksandra and Pezo, Lato and Janić Hajnal, Elizabet and Popović Milovanović, Tatjana",
year = "2024",
abstract = "Infections with phytoplasma present one of the most significant biotic stresses influencing
plant health, growth, and production. The phytoplasma ‘Candidatus Phytoplasma solani’ infects
a variety of plant species. This pathogen impacts the physiological and morphological characteristics of plants causing stunting, yellowing, leaf curling, and other symptoms that can lead to significant economic losses. The aim of this study was to determine biochemical changes in peony (Paeonia tenuifolia L.), mint (Mentha × piperita L.), and dill (Anethum graveolens L.) induced by ‘Ca. Phytoplasma solani’ in Serbia as well as to predict the impact of the biotic stress using artificial neural network (ANN) modeling. The phylogenetic position of the Serbian ‘Ca. Phytoplasma solani’ strains originated from the tested hosts using 16S rRNA (peony and carrot strains) and plsC (mint and dill strains) sequences indicated by their genetic homogeneity despite the host of origin. Biochemical parameters significantly differed in asymptomatic and symptomatic plants, except for total anthocyanidins contents in dill and the capacity of peony and mint extracts to neutralize superoxide anions and hydroxyl radicals, respectively. Principal Component Analysis (PCA) showed a correlation between different chemical parameters and revealed a clear separation among the samples. Based on the ANN performance, the optimal number of hidden neurons for the calculation of TS, RG, PAL, LP, NBT, •OH, TP, TT, Tflav, Tpro, Tant, DPPH, and Car was nine (using MLP 8-9-13), as it produced high r2 values (1.000 during the training period) and low SOS values. Developing an effective early warning system for the detection of plant diseases in different plant species is critical for improving crop yield and quality.",
publisher = "MDPI",
journal = "Horticulturae",
title = "The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach",
number = "5",
pages = "426",
volume = "10",
doi = "10.3390/horticulturae10050426"
}

Djalović, I., Mitrović, P., Trivan, G., Jelušić, A., Pezo, L., Janić Hajnal, E.,& Popović Milovanović, T.. (2024). The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach. in Horticulturae
MDPI., 10(5), 426.
https://doi.org/10.3390/horticulturae10050426

Djalović I, Mitrović P, Trivan G, Jelušić A, Pezo L, Janić Hajnal E, Popović Milovanović T. The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach. in Horticulturae. 2024;10(5):426.
doi:10.3390/horticulturae10050426 .

Djalović, Ivica, Mitrović, Petar, Trivan, Goran, Jelušić, Aleksandra, Pezo, Lato, Janić Hajnal, Elizabet, Popović Milovanović, Tatjana, "The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach" in Horticulturae, 10, no. 5 (2024):426,
https://doi.org/10.3390/horticulturae10050426 . .

TY  - JOUR
AU  - Mitrović, Petar
AU  - Djalović, Ivica
AU  - Kiprovski, Biljana
AU  - Veljović-Jovanović, Sonja
AU  - Trkulja, Vojislav
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1459
AB  - The present study examined the effects of Candidatus Phytoplasma solani infection on antioxidative metabolism in leaves and roots of carrot (Daucus carota L.). Disease symptoms appeared at the end of June in the form of the chlorosis on some of the leaves, which became intensely red one week later, while the previously healthy leaves from the same branch becme chlorotic. A few days later, all leaves from the infected leaf branch were intensely red. Infected plants also had slower growth compared to the healthy ones with fewer leaf branches developed. The roots of infected plants were less developed, seared, or gummy with or without brown-colored root hair. The presence of the pathogen was detected by sequencing the 16S rRNA. National Center for Biotechnology Information (NCBI) BLAST analyses of the obtained sequence revealed 100% identity of tested strain with deposited Ca. Phytoplasma solani strains from various countries and hosts, all belonging to the "stolbur" group (16SrXII-A). Identity of 99.74% was found when the tested Serbian strain (MF503627) was compared with the reference stolbur strain STOL11 (AF248959). The oxidative damage of membranes in carrot cells was accompanied by a decrease in the content of photosynthetic pigments. Furthermore, for the determination of specific scavenging properties of the extracts, in vitro antioxidant assay was performed. In phytoplasma-infected carrot leaves, there was a greater reduction in the level of glutathione content (GSH); however; flavonoids and anthocyanidins seem to be responsible for the accompanied increased antioxidative capacity against hydroxyl radical and hydrogen peroxide.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani
IS  - 2
VL  - 10
DO  - 10.3390/plants10020337
ER  - 

@article{
author = "Mitrović, Petar and Djalović, Ivica and Kiprovski, Biljana and Veljović-Jovanović, Sonja and Trkulja, Vojislav and Jelušić, Aleksandra and Popović, Tatjana",
year = "2021",
abstract = "The present study examined the effects of Candidatus Phytoplasma solani infection on antioxidative metabolism in leaves and roots of carrot (Daucus carota L.). Disease symptoms appeared at the end of June in the form of the chlorosis on some of the leaves, which became intensely red one week later, while the previously healthy leaves from the same branch becme chlorotic. A few days later, all leaves from the infected leaf branch were intensely red. Infected plants also had slower growth compared to the healthy ones with fewer leaf branches developed. The roots of infected plants were less developed, seared, or gummy with or without brown-colored root hair. The presence of the pathogen was detected by sequencing the 16S rRNA. National Center for Biotechnology Information (NCBI) BLAST analyses of the obtained sequence revealed 100% identity of tested strain with deposited Ca. Phytoplasma solani strains from various countries and hosts, all belonging to the "stolbur" group (16SrXII-A). Identity of 99.74% was found when the tested Serbian strain (MF503627) was compared with the reference stolbur strain STOL11 (AF248959). The oxidative damage of membranes in carrot cells was accompanied by a decrease in the content of photosynthetic pigments. Furthermore, for the determination of specific scavenging properties of the extracts, in vitro antioxidant assay was performed. In phytoplasma-infected carrot leaves, there was a greater reduction in the level of glutathione content (GSH); however; flavonoids and anthocyanidins seem to be responsible for the accompanied increased antioxidative capacity against hydroxyl radical and hydrogen peroxide.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani",
number = "2",
volume = "10",
doi = "10.3390/plants10020337"
}

Mitrović, P., Djalović, I., Kiprovski, B., Veljović-Jovanović, S., Trkulja, V., Jelušić, A.,& Popović, T.. (2021). Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani. in Plants-Basel
MDPI, Basel., 10(2).
https://doi.org/10.3390/plants10020337

Mitrović P, Djalović I, Kiprovski B, Veljović-Jovanović S, Trkulja V, Jelušić A, Popović T. Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani. in Plants-Basel. 2021;10(2).
doi:10.3390/plants10020337 .

Mitrović, Petar, Djalović, Ivica, Kiprovski, Biljana, Veljović-Jovanović, Sonja, Trkulja, Vojislav, Jelušić, Aleksandra, Popović, Tatjana, "Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani" in Plants-Basel, 10, no. 2 (2021),
https://doi.org/10.3390/plants10020337 . .