TY  - JOUR
AU  - Štrbac, Filip
AU  - Krnjajić, Slobodan
AU  - Maurelli, Maria Paola
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Ratajac, Radomir
AU  - Petrović, Kosta
AU  - Knežević, Goran
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
AU  - Bosco, Antonio
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1884
AB  - The development of anthelmintic resistance in sheep gastrointestinal nematodes (GINs) requires novel strategies for the sustainable control of these parasites. This study aimed to evaluate the anthelmintic efficacy of the Origanum vulgare (L.) essential oil (EO) against sheep GINs and to evaluate the possibility of its use in control practice. The in vitro egg hatch test was conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025, and 0.0125 mg/mL) of the tested EO. For the in vivo fecal egg count reduction test, the EO of O. vulgare was administrated orally at a mean single dose of 150 mg/kg to sheep from two farms in Southern Italy, whereby potential toxic effects to the hosts were also evaluated. In the egg hatch test, the inhibition of egg hatchability varied from 71.3% to 93.7%, depending on the concentration used. The high anthelmintic potential was confirmed in the fecal egg count reduction test with an average reduction of nematode eggs in feces of 43.2% and 60.1% on days 7 and 14 after treatment, respectively. In addition, no toxic effects were noticed during the clinical examination of sheep or by observing blood count and liver or kidney function test results. The obtained results suggest the strong activity of the O. vulgare EO against sheep GINs, probably due to a high percentage of carvacrol (76.21%), whereby it can be considered safe for sheep at the dose tested in vivo. Therefore, it is suitable for use in veterinary practice as a part of an integrated strategy for the control of sheep GINs.
PB  - MDPI
T2  - Animals
T1  - A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep
IS  - 1
SP  - 45
VL  - 13
DO  - 10.3390/ani13010045
ER  - 

@article{
author = "Štrbac, Filip and Krnjajić, Slobodan and Maurelli, Maria Paola and Stojanović, Dragica and Simin, Nataša and Orčić, Dejan and Ratajac, Radomir and Petrović, Kosta and Knežević, Goran and Cringoli, Giuseppe and Rinaldi, Laura and Bosco, Antonio",
year = "2022",
abstract = "The development of anthelmintic resistance in sheep gastrointestinal nematodes (GINs) requires novel strategies for the sustainable control of these parasites. This study aimed to evaluate the anthelmintic efficacy of the Origanum vulgare (L.) essential oil (EO) against sheep GINs and to evaluate the possibility of its use in control practice. The in vitro egg hatch test was conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025, and 0.0125 mg/mL) of the tested EO. For the in vivo fecal egg count reduction test, the EO of O. vulgare was administrated orally at a mean single dose of 150 mg/kg to sheep from two farms in Southern Italy, whereby potential toxic effects to the hosts were also evaluated. In the egg hatch test, the inhibition of egg hatchability varied from 71.3% to 93.7%, depending on the concentration used. The high anthelmintic potential was confirmed in the fecal egg count reduction test with an average reduction of nematode eggs in feces of 43.2% and 60.1% on days 7 and 14 after treatment, respectively. In addition, no toxic effects were noticed during the clinical examination of sheep or by observing blood count and liver or kidney function test results. The obtained results suggest the strong activity of the O. vulgare EO against sheep GINs, probably due to a high percentage of carvacrol (76.21%), whereby it can be considered safe for sheep at the dose tested in vivo. Therefore, it is suitable for use in veterinary practice as a part of an integrated strategy for the control of sheep GINs.",
publisher = "MDPI",
journal = "Animals",
title = "A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep",
number = "1",
pages = "45",
volume = "13",
doi = "10.3390/ani13010045"
}

Štrbac, F., Krnjajić, S., Maurelli, M. P., Stojanović, D., Simin, N., Orčić, D., Ratajac, R., Petrović, K., Knežević, G., Cringoli, G., Rinaldi, L.,& Bosco, A.. (2022). A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep. in Animals
MDPI., 13(1), 45.
https://doi.org/10.3390/ani13010045

Štrbac F, Krnjajić S, Maurelli MP, Stojanović D, Simin N, Orčić D, Ratajac R, Petrović K, Knežević G, Cringoli G, Rinaldi L, Bosco A. A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep. in Animals. 2022;13(1):45.
doi:10.3390/ani13010045 .

Štrbac, Filip, Krnjajić, Slobodan, Maurelli, Maria Paola, Stojanović, Dragica, Simin, Nataša, Orčić, Dejan, Ratajac, Radomir, Petrović, Kosta, Knežević, Goran, Cringoli, Giuseppe, Rinaldi, Laura, Bosco, Antonio, "A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep" in Animals, 13, no. 1 (2022):45,
https://doi.org/10.3390/ani13010045 . .