TY  - JOUR
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Maurelli, Maria Paola
AU  - Ratajac, Radomir
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Orcic, Dejan
AU  - Pusic, Ivan
AU  - Krnjajić, Slobodan
AU  - Sotiraki, Smaragda
AU  - Saralli, Giorgio
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1548
AB  - Herbal products such as essential oils may play a promising role in the treatment of infections caused by gastrointestinal nematodes (GINs). The aim of this study was to evaluate the in vitro potential of 11 essential oils (EOs) and one binary combination of isolated EO compounds, as well as the in vivo anthelmintic efficacy of two EO formulations. Four GIN genera were identified in the coproculture examination: Haemonchus, Trichostrongylus, Teladorsagia and Chabertia. The in vitro egg hatch test (EHT) was performed at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL) for each EO, whereas in the in vivo faecal egg count reduction test (FECRT), each EO sample was diluted in sunflower oil and orally administrated at a dose of 100 mg/kg to the different group of animals. In the EHT, the EOs of Origanum vulgare, Foeniculum vulgare, Satureja montana, Satureja hortensis and two types of Thymus vulgaris were the most effective. The dominant compounds of these EOs were carvacrol, thymol, anethol, p-cymene and gamma-terpinene, indicating their importance for the anthelmintic activity. In the FECRT, both T. vulgaris EO type 1 and linalool:estragole combination show an anthelmintic potential with a mean effect on FECR of approximately 25%. The results suggest the possible role of tested EOs as anthelmintic agents in sheep farms, although further in vivo tests are needed.
PB  - MDPI, Basel
T2  - Veterinary Sciences
T1  - Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep-In Vitro and In Vivo Studies
IS  - 2
VL  - 9
DO  - 10.3390/vetsci9020093
ER  - 

@article{
author = "Štrbac, Filip and Bosco, Antonio and Maurelli, Maria Paola and Ratajac, Radomir and Stojanović, Dragica and Simin, Nataša and Orcic, Dejan and Pusic, Ivan and Krnjajić, Slobodan and Sotiraki, Smaragda and Saralli, Giorgio and Cringoli, Giuseppe and Rinaldi, Laura",
year = "2022",
abstract = "Herbal products such as essential oils may play a promising role in the treatment of infections caused by gastrointestinal nematodes (GINs). The aim of this study was to evaluate the in vitro potential of 11 essential oils (EOs) and one binary combination of isolated EO compounds, as well as the in vivo anthelmintic efficacy of two EO formulations. Four GIN genera were identified in the coproculture examination: Haemonchus, Trichostrongylus, Teladorsagia and Chabertia. The in vitro egg hatch test (EHT) was performed at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL) for each EO, whereas in the in vivo faecal egg count reduction test (FECRT), each EO sample was diluted in sunflower oil and orally administrated at a dose of 100 mg/kg to the different group of animals. In the EHT, the EOs of Origanum vulgare, Foeniculum vulgare, Satureja montana, Satureja hortensis and two types of Thymus vulgaris were the most effective. The dominant compounds of these EOs were carvacrol, thymol, anethol, p-cymene and gamma-terpinene, indicating their importance for the anthelmintic activity. In the FECRT, both T. vulgaris EO type 1 and linalool:estragole combination show an anthelmintic potential with a mean effect on FECR of approximately 25%. The results suggest the possible role of tested EOs as anthelmintic agents in sheep farms, although further in vivo tests are needed.",
publisher = "MDPI, Basel",
journal = "Veterinary Sciences",
title = "Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep-In Vitro and In Vivo Studies",
number = "2",
volume = "9",
doi = "10.3390/vetsci9020093"
}

Štrbac, F., Bosco, A., Maurelli, M. P., Ratajac, R., Stojanović, D., Simin, N., Orcic, D., Pusic, I., Krnjajić, S., Sotiraki, S., Saralli, G., Cringoli, G.,& Rinaldi, L.. (2022). Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep-In Vitro and In Vivo Studies. in Veterinary Sciences
MDPI, Basel., 9(2).
https://doi.org/10.3390/vetsci9020093

Štrbac F, Bosco A, Maurelli MP, Ratajac R, Stojanović D, Simin N, Orcic D, Pusic I, Krnjajić S, Sotiraki S, Saralli G, Cringoli G, Rinaldi L. Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep-In Vitro and In Vivo Studies. in Veterinary Sciences. 2022;9(2).
doi:10.3390/vetsci9020093 .

Štrbac, Filip, Bosco, Antonio, Maurelli, Maria Paola, Ratajac, Radomir, Stojanović, Dragica, Simin, Nataša, Orcic, Dejan, Pusic, Ivan, Krnjajić, Slobodan, Sotiraki, Smaragda, Saralli, Giorgio, Cringoli, Giuseppe, Rinaldi, Laura, "Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep-In Vitro and In Vivo Studies" in Veterinary Sciences, 9, no. 2 (2022),
https://doi.org/10.3390/vetsci9020093 . .

TY  - JOUR
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Amadesi, Alessandra
AU  - Rinaldi, Laura
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Orcic, Dejan
AU  - Pusic, Ivan
AU  - Krnjajić, Slobodan
AU  - Ratajac, Radomir
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1393
AB  - The development of resistance to commercial anthelmintics, particularly in different species of gastrointestinal nematodes (GINs), requires the search for alternatives. Within that context, the aim of this study was to evaluate the in vitro ovicidal activity of five different essential oils (EOs): Origanum vulgare, Satureja hortensis, Thymus vulgaris, Mentha x piperita and Helichrysum arenarium against sheep GINs. For this purpose, the nematode eggs were collected from naturally infected sheep by GINs in two farms located in southern Italy. The egg hatch test (EHT) was performed at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL) for each EO. Gas chromatography-mass spectrometry chemical analyses of tested EOs, as well as coproculture examination of tested faecal samples, were also conducted. The results of EHT showed the greatest ovicidal activity of O. vulgare EO with a maximum effect on egg hatching (100%) for all tested concentrations. A similar effect was also shown by S. hortensis and T. vulgaris EOs with an activity of 99.3-100% and 98.5-100%, respectively. M. piperita EO showed medium, dose-dependent ovicidal activity with an inhibitory effect of 72.5-99.8% on the egg hatchability, while the least effective was H. arenarium EO with an activity of 59.8-69.3%. For the anthelmintic activities of the tested EOs are responsible their ingredients, above all carvacrol, thymol, p-cymene and gamma-terpinene. The present study demonstrated the significant anthelmintic potential of the tested EOs and emphasizes the possible importance of medicinal plant products for the control of gastrointestinal parasites in sheep.
PB  - Univ Agriculture, Fac Veterinary Science, Faisalabad
T2  - Pakistan Veterinary Journal
T1  - Ovicidal Potential of Five Different Essential Oils to Control Gastrointestinal Nematodes of Sheep
EP  - 358
IS  - 3
SP  - 353
VL  - 41
DO  - 10.29261/pakvetj/2021.026
ER  - 

@article{
author = "Štrbac, Filip and Bosco, Antonio and Amadesi, Alessandra and Rinaldi, Laura and Stojanović, Dragica and Simin, Nataša and Orcic, Dejan and Pusic, Ivan and Krnjajić, Slobodan and Ratajac, Radomir",
year = "2020",
abstract = "The development of resistance to commercial anthelmintics, particularly in different species of gastrointestinal nematodes (GINs), requires the search for alternatives. Within that context, the aim of this study was to evaluate the in vitro ovicidal activity of five different essential oils (EOs): Origanum vulgare, Satureja hortensis, Thymus vulgaris, Mentha x piperita and Helichrysum arenarium against sheep GINs. For this purpose, the nematode eggs were collected from naturally infected sheep by GINs in two farms located in southern Italy. The egg hatch test (EHT) was performed at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL) for each EO. Gas chromatography-mass spectrometry chemical analyses of tested EOs, as well as coproculture examination of tested faecal samples, were also conducted. The results of EHT showed the greatest ovicidal activity of O. vulgare EO with a maximum effect on egg hatching (100%) for all tested concentrations. A similar effect was also shown by S. hortensis and T. vulgaris EOs with an activity of 99.3-100% and 98.5-100%, respectively. M. piperita EO showed medium, dose-dependent ovicidal activity with an inhibitory effect of 72.5-99.8% on the egg hatchability, while the least effective was H. arenarium EO with an activity of 59.8-69.3%. For the anthelmintic activities of the tested EOs are responsible their ingredients, above all carvacrol, thymol, p-cymene and gamma-terpinene. The present study demonstrated the significant anthelmintic potential of the tested EOs and emphasizes the possible importance of medicinal plant products for the control of gastrointestinal parasites in sheep.",
publisher = "Univ Agriculture, Fac Veterinary Science, Faisalabad",
journal = "Pakistan Veterinary Journal",
title = "Ovicidal Potential of Five Different Essential Oils to Control Gastrointestinal Nematodes of Sheep",
pages = "358-353",
number = "3",
volume = "41",
doi = "10.29261/pakvetj/2021.026"
}

Štrbac, F., Bosco, A., Amadesi, A., Rinaldi, L., Stojanović, D., Simin, N., Orcic, D., Pusic, I., Krnjajić, S.,& Ratajac, R.. (2020). Ovicidal Potential of Five Different Essential Oils to Control Gastrointestinal Nematodes of Sheep. in Pakistan Veterinary Journal
Univ Agriculture, Fac Veterinary Science, Faisalabad., 41(3), 353-358.
https://doi.org/10.29261/pakvetj/2021.026

Štrbac F, Bosco A, Amadesi A, Rinaldi L, Stojanović D, Simin N, Orcic D, Pusic I, Krnjajić S, Ratajac R. Ovicidal Potential of Five Different Essential Oils to Control Gastrointestinal Nematodes of Sheep. in Pakistan Veterinary Journal. 2020;41(3):353-358.
doi:10.29261/pakvetj/2021.026 .

Štrbac, Filip, Bosco, Antonio, Amadesi, Alessandra, Rinaldi, Laura, Stojanović, Dragica, Simin, Nataša, Orcic, Dejan, Pusic, Ivan, Krnjajić, Slobodan, Ratajac, Radomir, "Ovicidal Potential of Five Different Essential Oils to Control Gastrointestinal Nematodes of Sheep" in Pakistan Veterinary Journal, 41, no. 3 (2020):353-358,
https://doi.org/10.29261/pakvetj/2021.026 . .