TY  - JOUR
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
AU  - Barać, Goran
AU  - Nikolić, Dušan
AU  - Stošić, Nemanja
AU  - Scortichini, Marco
AU  - Popović Milovanović, Tatjana
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3206
AB  - In August 2020, the unusual appearance of crown gall symptoms was observed on the
tobacco plants (hybrid PVH2310) grown in fields in the Golubinci (Srem district, Serbia) locality. The causal agent isolated from galls located on tobacco roots formed circular, convex, and glistening light blue colonies, and then dark to olive-green-colored bacterial colonies on a semi-selective D1 medium. Molecular analysis based on multiplex PCR and multi-locus sequence analysis (MLSA) using concatenated sequences of the atpD, dnaK, glnA, and rpoB genes as well as 16S rRNA identified Serbian tobacco isolates such as Agrobacterium tumefaciens (biovar 1). Two duplex PCR methods confirmed the presence of the virD2 and virC genes in tobacco isolates. Pathogenicity tests performed on carrot discs and squash fruits resulted in tumor/gall formation after 12 to 16 days post inoculation, respectively. Pathogenicity was also confirmed on tobacco plants, where isolates caused tumor development 21−25 days after inoculation. API 50 CH generated results regarding the biochemical features of the Serbian tobacco isolates. As A. tumefaciens (biovar 1) as a cause of tobacco crown gall has previously been documented solely in Japan, there is presently no data on its wider occurrence. Therefore, this first detailed investigation of A. tumefaciens isolated from naturally infected tobacco in Serbia will contribute to a better understanding of it at the global level.
PB  - MDPI
T2  - Agronomy
T1  - In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia
IS  - 4
SP  - 851
VL  - 14
DO  - 10.3390/agronomy14040851
ER  - 

@article{
author = "Iličić, Renata and Jelušić, Aleksandra and Barać, Goran and Nikolić, Dušan and Stošić, Nemanja and Scortichini, Marco and Popović Milovanović, Tatjana",
year = "2024",
abstract = "In August 2020, the unusual appearance of crown gall symptoms was observed on the
tobacco plants (hybrid PVH2310) grown in fields in the Golubinci (Srem district, Serbia) locality. The causal agent isolated from galls located on tobacco roots formed circular, convex, and glistening light blue colonies, and then dark to olive-green-colored bacterial colonies on a semi-selective D1 medium. Molecular analysis based on multiplex PCR and multi-locus sequence analysis (MLSA) using concatenated sequences of the atpD, dnaK, glnA, and rpoB genes as well as 16S rRNA identified Serbian tobacco isolates such as Agrobacterium tumefaciens (biovar 1). Two duplex PCR methods confirmed the presence of the virD2 and virC genes in tobacco isolates. Pathogenicity tests performed on carrot discs and squash fruits resulted in tumor/gall formation after 12 to 16 days post inoculation, respectively. Pathogenicity was also confirmed on tobacco plants, where isolates caused tumor development 21−25 days after inoculation. API 50 CH generated results regarding the biochemical features of the Serbian tobacco isolates. As A. tumefaciens (biovar 1) as a cause of tobacco crown gall has previously been documented solely in Japan, there is presently no data on its wider occurrence. Therefore, this first detailed investigation of A. tumefaciens isolated from naturally infected tobacco in Serbia will contribute to a better understanding of it at the global level.",
publisher = "MDPI",
journal = "Agronomy",
title = "In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia",
number = "4",
pages = "851",
volume = "14",
doi = "10.3390/agronomy14040851"
}

Iličić, R., Jelušić, A., Barać, G., Nikolić, D., Stošić, N., Scortichini, M.,& Popović Milovanović, T.. (2024). In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia. in Agronomy
MDPI., 14(4), 851.
https://doi.org/10.3390/agronomy14040851

Iličić R, Jelušić A, Barać G, Nikolić D, Stošić N, Scortichini M, Popović Milovanović T. In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia. in Agronomy. 2024;14(4):851.
doi:10.3390/agronomy14040851 .

Iličić, Renata, Jelušić, Aleksandra, Barać, Goran, Nikolić, Dušan, Stošić, Nemanja, Scortichini, Marco, Popović Milovanović, Tatjana, "In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia" in Agronomy, 14, no. 4 (2024):851,
https://doi.org/10.3390/agronomy14040851 . .

TY  - CONF
AU  - Iličić, Renata
AU  - Barać, Goran
AU  - Marković, Sanja
AU  - Jelušić, Aleksandra
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1949
AB  - BACKGROUND
Bačka (Vojvodina, Serbia) is one of the main potato-growing areas in Serbia. The presence of the stolbur-associated 
symptoms in the form of reddening and leaf rolling, shortened internodes, and aerial tuber formation were 
frequently noticed on the potato plants. Therefore, the identification of the causal agent of the disease was 
initiated, considering that presence of phytoplasmas was not earlier reported in Maglić locality.
OBJECTIVES
This research aimed to identify the causal agent of the phytoplasmas’ disease in potato field (unknown cultivar) 
in locality Maglić (Bačka) in 2021. 
METHODS 
Samples for the identification within the survey site consisted of pooled multiple diseased plants (leaves 
and tubers). Genomic DNA from the ten pooled samples was extracted using the Cetyltrimethyl ammonium 
bromide (CTAB) method. Identification was performed with polymerase chain reaction (PCR) using universal 
phytoplasma 16S rRNA primer pair P1/P7. The obtained sequences were checked for identity with the deposited 
strains in the NCBI database using the BLASTn search tool. Further, Neighbour-joining (NJ) tree was made to 
reconstruct the phylogeny of potato strains in relation to deposited strains in the NCBI database.
RESULTS 
PCR amplification performed with primer pair P1/P7 resulted in amplicon generation at 1800 bp for three 
(two from tubers and one from leaves) out of a total of ten tested samples. BLASTn analysis revealed 100% 
identity of potato strains with deposited strains of Candidatus Phytoplasma solani, the 16SrXII-A ribosom al subgroup. NJ phylogenetic analysis grouped potato strains in the same tree cluster with NCBI deposited 
strains of Ca. Phytoplasma solani.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology
T1  - NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA   SOLANI ON POTATO IN VOJVODINA PROVINCE
SP  - 916
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1949
ER  - 

@conference{
author = "Iličić, Renata and Barać, Goran and Marković, Sanja and Jelušić, Aleksandra and Stanković, Slaviša and Popović, Tatjana",
year = "2022",
abstract = "BACKGROUND
Bačka (Vojvodina, Serbia) is one of the main potato-growing areas in Serbia. The presence of the stolbur-associated 
symptoms in the form of reddening and leaf rolling, shortened internodes, and aerial tuber formation were 
frequently noticed on the potato plants. Therefore, the identification of the causal agent of the disease was 
initiated, considering that presence of phytoplasmas was not earlier reported in Maglić locality.
OBJECTIVES
This research aimed to identify the causal agent of the phytoplasmas’ disease in potato field (unknown cultivar) 
in locality Maglić (Bačka) in 2021. 
METHODS 
Samples for the identification within the survey site consisted of pooled multiple diseased plants (leaves 
and tubers). Genomic DNA from the ten pooled samples was extracted using the Cetyltrimethyl ammonium 
bromide (CTAB) method. Identification was performed with polymerase chain reaction (PCR) using universal 
phytoplasma 16S rRNA primer pair P1/P7. The obtained sequences were checked for identity with the deposited 
strains in the NCBI database using the BLASTn search tool. Further, Neighbour-joining (NJ) tree was made to 
reconstruct the phylogeny of potato strains in relation to deposited strains in the NCBI database.
RESULTS 
PCR amplification performed with primer pair P1/P7 resulted in amplicon generation at 1800 bp for three 
(two from tubers and one from leaves) out of a total of ten tested samples. BLASTn analysis revealed 100% 
identity of potato strains with deposited strains of Candidatus Phytoplasma solani, the 16SrXII-A ribosom al subgroup. NJ phylogenetic analysis grouped potato strains in the same tree cluster with NCBI deposited 
strains of Ca. Phytoplasma solani.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology",
title = "NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA   SOLANI ON POTATO IN VOJVODINA PROVINCE",
pages = "916",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1949"
}

Iličić, R., Barać, G., Marković, S., Jelušić, A., Stanković, S.,& Popović, T.. (2022). NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA   SOLANI ON POTATO IN VOJVODINA PROVINCE. in FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology., 916.
https://hdl.handle.net/21.15107/rcub_rimsi_1949

Iličić R, Barać G, Marković S, Jelušić A, Stanković S, Popović T. NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA   SOLANI ON POTATO IN VOJVODINA PROVINCE. in FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology. 2022;:916.
https://hdl.handle.net/21.15107/rcub_rimsi_1949 .

Iličić, Renata, Barać, Goran, Marković, Sanja, Jelušić, Aleksandra, Stanković, Slaviša, Popović, Tatjana, "NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA   SOLANI ON POTATO IN VOJVODINA PROVINCE" in FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology (2022):916,
https://hdl.handle.net/21.15107/rcub_rimsi_1949 .