TY  - CONF
AU  - Dimkić, Ivica
AU  - Jelušić, Aleksandra
AU  - Janakiev, Tamara
AU  - Bandelj, Dunja
AU  - Vukićević, Slavoljub
AU  - Hladnik, Matjaž
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2042
AB  - Numerous microbial taxa are used as biofertilizers because they have the potential to competitively colonize the rhizosphere or root interior of plants and promote nutrient uptake. The aim was to investigate the shifts of keystone bacterial species in maize roots and rhizosphere during seedling stage, flowering, and harvest, under different treatments of bacterial phytobiotic (PHY), poultry
manure (PM) and their combination (PHY_PM). Soil bacterial communities remained largely unchanged regardless of the treatment applied or phenophase studied, with uncultured Gaiellales and Bacillus being the most abundant. In contrast, bacterial communities in roots differed in terms of distribution and relative abundance (RA) of different taxa between growth stages and treatments. The most abundant bacterial taxa in the roots during the initial seedling stage was
Pseudomonas. In the flowering, Bacillus occurred with a two- to threefold higher RA in treatments with PHY or PM compared to the negative control, while Lechevalieria dominated during the harvest. Differential abundance analysis at the seedling stage showed a reduction of Pseudomonas in roots treated with PHY, while Pseudonocardia was significantly more abundant in roots treated with PM than in the other samples. Massilia, Streptomyces, Lechevalieria, Microbacteriaceae, Aeromicrobium, Sphingomonas and Roseiflexaceae were significantly higher in the PHY_PM treated root samples. Steroidobacter and Bdellovibrio were absent in PHY and PM treated root samples during flowering, while Bdellovibrio was significantly reduced in PHY_PM treatment compared to the negative control. RA of Bradyrhizobium and Polaromonas was reduced in PHY treated root samples, and Dongia was completely absent. Gaiellales was significantly more abundant, while Sphingomonas was less abundant in roots treated with PM and PHY_PM. Solirubrobacter and Bdellovibrio were absent from roots during harvest in all three treatment types. Pajaroellobacter was completely absent in PHY and PM treatments, while Steroidobacteraceae were present only in PHY and Cyclobacteriaceae in PM. Mucilaginibacter calamicampi showed
significantly higher RA in PHY_PM treatment, while Sphingomonas was reduced. Considering the non-disruptive effect of PHY on the "core" bacteriobiota and the positive effects on the presence of beneficial bacterial genera, such products could be proposed as a promising alternative to chemicals and organic fertilizers in maize cultivation.
PB  - Croatian Microbiological Society, Zagreb, Croatia
C3  - Power of Microbes in Industry and Environment, Poreč, Croatia
T1  - Differential Abundance Analysis of "Core" Bacteriobiota During Key Growth Stages of Maize
EP  - 45
IS  - OP8
SP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2042
ER  - 

@conference{
author = "Dimkić, Ivica and Jelušić, Aleksandra and Janakiev, Tamara and Bandelj, Dunja and Vukićević, Slavoljub and Hladnik, Matjaž",
year = "2023",
abstract = "Numerous microbial taxa are used as biofertilizers because they have the potential to competitively colonize the rhizosphere or root interior of plants and promote nutrient uptake. The aim was to investigate the shifts of keystone bacterial species in maize roots and rhizosphere during seedling stage, flowering, and harvest, under different treatments of bacterial phytobiotic (PHY), poultry
manure (PM) and their combination (PHY_PM). Soil bacterial communities remained largely unchanged regardless of the treatment applied or phenophase studied, with uncultured Gaiellales and Bacillus being the most abundant. In contrast, bacterial communities in roots differed in terms of distribution and relative abundance (RA) of different taxa between growth stages and treatments. The most abundant bacterial taxa in the roots during the initial seedling stage was
Pseudomonas. In the flowering, Bacillus occurred with a two- to threefold higher RA in treatments with PHY or PM compared to the negative control, while Lechevalieria dominated during the harvest. Differential abundance analysis at the seedling stage showed a reduction of Pseudomonas in roots treated with PHY, while Pseudonocardia was significantly more abundant in roots treated with PM than in the other samples. Massilia, Streptomyces, Lechevalieria, Microbacteriaceae, Aeromicrobium, Sphingomonas and Roseiflexaceae were significantly higher in the PHY_PM treated root samples. Steroidobacter and Bdellovibrio were absent in PHY and PM treated root samples during flowering, while Bdellovibrio was significantly reduced in PHY_PM treatment compared to the negative control. RA of Bradyrhizobium and Polaromonas was reduced in PHY treated root samples, and Dongia was completely absent. Gaiellales was significantly more abundant, while Sphingomonas was less abundant in roots treated with PM and PHY_PM. Solirubrobacter and Bdellovibrio were absent from roots during harvest in all three treatment types. Pajaroellobacter was completely absent in PHY and PM treatments, while Steroidobacteraceae were present only in PHY and Cyclobacteriaceae in PM. Mucilaginibacter calamicampi showed
significantly higher RA in PHY_PM treatment, while Sphingomonas was reduced. Considering the non-disruptive effect of PHY on the "core" bacteriobiota and the positive effects on the presence of beneficial bacterial genera, such products could be proposed as a promising alternative to chemicals and organic fertilizers in maize cultivation.",
publisher = "Croatian Microbiological Society, Zagreb, Croatia",
journal = "Power of Microbes in Industry and Environment, Poreč, Croatia",
title = "Differential Abundance Analysis of "Core" Bacteriobiota During Key Growth Stages of Maize",
pages = "45-44",
number = "OP8",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2042"
}

Dimkić, I., Jelušić, A., Janakiev, T., Bandelj, D., Vukićević, S.,& Hladnik, M.. (2023). Differential Abundance Analysis of "Core" Bacteriobiota During Key Growth Stages of Maize. in Power of Microbes in Industry and Environment, Poreč, Croatia
Croatian Microbiological Society, Zagreb, Croatia.(OP8), 44-45.
https://hdl.handle.net/21.15107/rcub_rimsi_2042

Dimkić I, Jelušić A, Janakiev T, Bandelj D, Vukićević S, Hladnik M. Differential Abundance Analysis of "Core" Bacteriobiota During Key Growth Stages of Maize. in Power of Microbes in Industry and Environment, Poreč, Croatia. 2023;(OP8):44-45.
https://hdl.handle.net/21.15107/rcub_rimsi_2042 .

Dimkić, Ivica, Jelušić, Aleksandra, Janakiev, Tamara, Bandelj, Dunja, Vukićević, Slavoljub, Hladnik, Matjaž, "Differential Abundance Analysis of "Core" Bacteriobiota During Key Growth Stages of Maize" in Power of Microbes in Industry and Environment, Poreč, Croatia, no. OP8 (2023):44-45,
https://hdl.handle.net/21.15107/rcub_rimsi_2042 .

TY  - CONF
AU  - Dimkić, Ivica
AU  - Jelušić, Aleksandra
AU  - Hladnik, Matjaž
AU  - Janakiev, Tamara
AU  - Bandelj, Dunja
AU  - Vukićević, Slavoljub
AU  - Fira, Đorđe
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2041
AB  - Maize (Zea mays L.) occupies a significant part of the world cultivated area. Beneficial microorganisms and organic fertilizers are powerful tools for sustainable agricultural management. Applying appropriate formulation either through seed coating or composting material together with seeds during the sowing was aimed to achieve optimal performance on plants and increase the crop yields. Also, a complete analysis of phytobiome including high-throughput amplicon and whole-genome sequencing, and computational biology of the treated and non-treated plants' roots and soil was applied. The statistically significant difference in bacterial communities between phenophases and treatments was much more prevalent in the roots than soil itself. In general, seedling phenophase (II) differed in terms of diversity from bacterial communities of flowering (III) and harvesting phenophases (IV), regardless of treatment, indicating good colonization with the treated substrate for up to one month. In the root samples, the dominance of the genus Pseudomonas was evident, in the initial stages (phase II), while this percentage decreased linearly over time. High relative abundance of the genera Achromobacter, Sphingobacterium, and Stenotrophomonas also characterized II phenophase. Contrary, a relative abundance of the genus Bacillus was characteristic for III phenophase, and especially twice or three times higher in treatments with phytobiotic or manure compared to the negative control. Furthermore, it was observed that with increasing inoculation of Bacillus isolate, the percentage of Pantoea genus decreases drastically in coated seeds. In conclusion, a statistically significant increase in maize yield was obtained in the treatment with phytobiotic with an average value of 650 kg/ha compared to the negative control.
PB  - Istočno Sarajevo : Poljoprivredni fakultet
C3  - XIII International Scientific Agriculture Symposium "AGROSYM 2022", Jahorina, Bosnia and Herzegovina
T1  - Bioinoculants in sustainable agriculture – the influence on maize characteristics and phytobiome
EP  - 348
SP  - 348
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2041
ER  - 

@conference{
author = "Dimkić, Ivica and Jelušić, Aleksandra and Hladnik, Matjaž and Janakiev, Tamara and Bandelj, Dunja and Vukićević, Slavoljub and Fira, Đorđe",
year = "2022",
abstract = "Maize (Zea mays L.) occupies a significant part of the world cultivated area. Beneficial microorganisms and organic fertilizers are powerful tools for sustainable agricultural management. Applying appropriate formulation either through seed coating or composting material together with seeds during the sowing was aimed to achieve optimal performance on plants and increase the crop yields. Also, a complete analysis of phytobiome including high-throughput amplicon and whole-genome sequencing, and computational biology of the treated and non-treated plants' roots and soil was applied. The statistically significant difference in bacterial communities between phenophases and treatments was much more prevalent in the roots than soil itself. In general, seedling phenophase (II) differed in terms of diversity from bacterial communities of flowering (III) and harvesting phenophases (IV), regardless of treatment, indicating good colonization with the treated substrate for up to one month. In the root samples, the dominance of the genus Pseudomonas was evident, in the initial stages (phase II), while this percentage decreased linearly over time. High relative abundance of the genera Achromobacter, Sphingobacterium, and Stenotrophomonas also characterized II phenophase. Contrary, a relative abundance of the genus Bacillus was characteristic for III phenophase, and especially twice or three times higher in treatments with phytobiotic or manure compared to the negative control. Furthermore, it was observed that with increasing inoculation of Bacillus isolate, the percentage of Pantoea genus decreases drastically in coated seeds. In conclusion, a statistically significant increase in maize yield was obtained in the treatment with phytobiotic with an average value of 650 kg/ha compared to the negative control.",
publisher = "Istočno Sarajevo : Poljoprivredni fakultet",
journal = "XIII International Scientific Agriculture Symposium "AGROSYM 2022", Jahorina, Bosnia and Herzegovina",
title = "Bioinoculants in sustainable agriculture – the influence on maize characteristics and phytobiome",
pages = "348-348",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2041"
}

Dimkić, I., Jelušić, A., Hladnik, M., Janakiev, T., Bandelj, D., Vukićević, S.,& Fira, Đ.. (2022). Bioinoculants in sustainable agriculture – the influence on maize characteristics and phytobiome. in XIII International Scientific Agriculture Symposium "AGROSYM 2022", Jahorina, Bosnia and Herzegovina
Istočno Sarajevo : Poljoprivredni fakultet., 348-348.
https://hdl.handle.net/21.15107/rcub_rimsi_2041

Dimkić I, Jelušić A, Hladnik M, Janakiev T, Bandelj D, Vukićević S, Fira Đ. Bioinoculants in sustainable agriculture – the influence on maize characteristics and phytobiome. in XIII International Scientific Agriculture Symposium "AGROSYM 2022", Jahorina, Bosnia and Herzegovina. 2022;:348-348.
https://hdl.handle.net/21.15107/rcub_rimsi_2041 .

Dimkić, Ivica, Jelušić, Aleksandra, Hladnik, Matjaž, Janakiev, Tamara, Bandelj, Dunja, Vukićević, Slavoljub, Fira, Đorđe, "Bioinoculants in sustainable agriculture – the influence on maize characteristics and phytobiome" in XIII International Scientific Agriculture Symposium "AGROSYM 2022", Jahorina, Bosnia and Herzegovina (2022):348-348,
https://hdl.handle.net/21.15107/rcub_rimsi_2041 .