TY  - JOUR
AU  - Veljović-Jovanović, Sonja
AU  - Kukavica, Biljana
AU  - Navari-Izzo, Flavia
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/248
AB  - Resurrection plants are able to dehydrate/rehydrate rapidly without cell damage by a mechanism, the understanding of which may be of ecological importance in the adaptation of crop plants to dry conditions. The o-diphenol oxidase in Ramonda serbica Pan. & Petrov, a rare resurrection plant of the Balkan Peninsula, was characterized in respect to different isoforms, preferable substrates and specific inhibitors. Two anionic isoforms with pI 4.6 and 4.7 were separated from turgid leaves. Three additional anionic isoforms (pI 5.1, 5.3 and 5.6) and three neutral isoforms (pI from 6.8 to 7.4) were induced in desiccated leaves. Based on apparent K-m values, the affinity for reducing substrates decreased as follows: methyl catechol > chlorogenic acid > 3,4-dihydroxyphenylalanine > caffeic acid > pyrogallol. Polyphenol oxidase (PPO) activity was specifically sensitive to diethyldithiocarbamate and also inhibited by KCN, DTT and salicylic hydroxamic acid but with no inhibitory effect of Na3N. Plants were subjected to drought-to-near complete water loss (approximately 2% relative water content, RWC) and several fold higher PPO activity was detected in desiccated leaves. Ramonda leaves contain high levels of phenolics, which decreased during drought. Rehydration of dry leaves from 2% RWC to 95% RWC led to transient inhibition of PPO in the first few hours. Within a day, the levels completely recovered to those determined in desiccated leaves. The finding of desiccation-induced high activity of PPO and new isoforms, which were also present in rehydrated turgid leaves, indicates a substantial role for PPO in the adaptation mechanism of resurrection plants to desiccation and also to the oxidative stress during rehydration.
PB  - Wiley, Hoboken
T2  - Physiologia Plantarum
T1  - Characterization of polyphenol oxidase changes induced by desiccation of Ramonda serbica leaves
EP  - 416
IS  - 4
SP  - 407
VL  - 132
DO  - 10.1111/j.1399-3054.2007.01040.x
ER  - 

@article{
author = "Veljović-Jovanović, Sonja and Kukavica, Biljana and Navari-Izzo, Flavia",
year = "2008",
abstract = "Resurrection plants are able to dehydrate/rehydrate rapidly without cell damage by a mechanism, the understanding of which may be of ecological importance in the adaptation of crop plants to dry conditions. The o-diphenol oxidase in Ramonda serbica Pan. & Petrov, a rare resurrection plant of the Balkan Peninsula, was characterized in respect to different isoforms, preferable substrates and specific inhibitors. Two anionic isoforms with pI 4.6 and 4.7 were separated from turgid leaves. Three additional anionic isoforms (pI 5.1, 5.3 and 5.6) and three neutral isoforms (pI from 6.8 to 7.4) were induced in desiccated leaves. Based on apparent K-m values, the affinity for reducing substrates decreased as follows: methyl catechol > chlorogenic acid > 3,4-dihydroxyphenylalanine > caffeic acid > pyrogallol. Polyphenol oxidase (PPO) activity was specifically sensitive to diethyldithiocarbamate and also inhibited by KCN, DTT and salicylic hydroxamic acid but with no inhibitory effect of Na3N. Plants were subjected to drought-to-near complete water loss (approximately 2% relative water content, RWC) and several fold higher PPO activity was detected in desiccated leaves. Ramonda leaves contain high levels of phenolics, which decreased during drought. Rehydration of dry leaves from 2% RWC to 95% RWC led to transient inhibition of PPO in the first few hours. Within a day, the levels completely recovered to those determined in desiccated leaves. The finding of desiccation-induced high activity of PPO and new isoforms, which were also present in rehydrated turgid leaves, indicates a substantial role for PPO in the adaptation mechanism of resurrection plants to desiccation and also to the oxidative stress during rehydration.",
publisher = "Wiley, Hoboken",
journal = "Physiologia Plantarum",
title = "Characterization of polyphenol oxidase changes induced by desiccation of Ramonda serbica leaves",
pages = "416-407",
number = "4",
volume = "132",
doi = "10.1111/j.1399-3054.2007.01040.x"
}

Veljović-Jovanović, S., Kukavica, B.,& Navari-Izzo, F.. (2008). Characterization of polyphenol oxidase changes induced by desiccation of Ramonda serbica leaves. in Physiologia Plantarum
Wiley, Hoboken., 132(4), 407-416.
https://doi.org/10.1111/j.1399-3054.2007.01040.x

Veljović-Jovanović S, Kukavica B, Navari-Izzo F. Characterization of polyphenol oxidase changes induced by desiccation of Ramonda serbica leaves. in Physiologia Plantarum. 2008;132(4):407-416.
doi:10.1111/j.1399-3054.2007.01040.x .

Veljović-Jovanović, Sonja, Kukavica, Biljana, Navari-Izzo, Flavia, "Characterization of polyphenol oxidase changes induced by desiccation of Ramonda serbica leaves" in Physiologia Plantarum, 132, no. 4 (2008):407-416,
https://doi.org/10.1111/j.1399-3054.2007.01040.x . .

TY  - JOUR
AU  - Kukavica, Biljana
AU  - Quartacci, M. F.
AU  - Veljović-Jovanović, Sonja
AU  - Navari-Izzo, Flavia
PY  - 2007
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/198
AB  - Plasma membrane was isolated from roots of pea and maize plants and used to analyze POD and SOD isoforms, as well as lipid composition. Among lipids, phospholipids were the main lipid class, with phosphatidylcholine being the most abundant individual component in both pea and maize plasma membranes. Significant differences between the two plant species were found in the contents of cerebrosides, free sterols, and steryl glycosides. Most maize POD isoforms were with neutral and anionic pI values, but the opposite was observed in pea. While both anionic and cationic SOD isoforms were isolated from maize, only two anionic SOD isoforms were detected in pea.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Lipid composition of pea (Pisum sativum L.) and maize (Zea mays L.) root plasma membrane and membrane-bound peroxidase and superoxide dismutase
EP  - 302
IS  - 4
SP  - 295
VL  - 59
DO  - 10.2298/ABS0704295K
ER  - 

@article{
author = "Kukavica, Biljana and Quartacci, M. F. and Veljović-Jovanović, Sonja and Navari-Izzo, Flavia",
year = "2007",
abstract = "Plasma membrane was isolated from roots of pea and maize plants and used to analyze POD and SOD isoforms, as well as lipid composition. Among lipids, phospholipids were the main lipid class, with phosphatidylcholine being the most abundant individual component in both pea and maize plasma membranes. Significant differences between the two plant species were found in the contents of cerebrosides, free sterols, and steryl glycosides. Most maize POD isoforms were with neutral and anionic pI values, but the opposite was observed in pea. While both anionic and cationic SOD isoforms were isolated from maize, only two anionic SOD isoforms were detected in pea.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Lipid composition of pea (Pisum sativum L.) and maize (Zea mays L.) root plasma membrane and membrane-bound peroxidase and superoxide dismutase",
pages = "302-295",
number = "4",
volume = "59",
doi = "10.2298/ABS0704295K"
}

Kukavica, B., Quartacci, M. F., Veljović-Jovanović, S.,& Navari-Izzo, F.. (2007). Lipid composition of pea (Pisum sativum L.) and maize (Zea mays L.) root plasma membrane and membrane-bound peroxidase and superoxide dismutase. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 59(4), 295-302.
https://doi.org/10.2298/ABS0704295K

Kukavica B, Quartacci MF, Veljović-Jovanović S, Navari-Izzo F. Lipid composition of pea (Pisum sativum L.) and maize (Zea mays L.) root plasma membrane and membrane-bound peroxidase and superoxide dismutase. in Archives of Biological Sciences. 2007;59(4):295-302.
doi:10.2298/ABS0704295K .

Kukavica, Biljana, Quartacci, M. F., Veljović-Jovanović, Sonja, Navari-Izzo, Flavia, "Lipid composition of pea (Pisum sativum L.) and maize (Zea mays L.) root plasma membrane and membrane-bound peroxidase and superoxide dismutase" in Archives of Biological Sciences, 59, no. 4 (2007):295-302,
https://doi.org/10.2298/ABS0704295K . .

TY  - JOUR
AU  - Veljović-Jovanović, Sonja
AU  - Kukavica, Biljana
AU  - Stevanović, Branka
AU  - Navari-Izzo, Flavia
PY  - 2006
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/154
AB  - Ramonda sp. (Gesneriaceae) is an endemic and relic plant in a very small group of poikilohydric angiosperms that are able to survive in an almost completely dehydrated state. Senescence- and drought-related changes in the activity of peroxidase (POD; EC 1.11.1.7), ascorbate peroxidase (EC 1.11.1.11), and superoxide dismutase (SOD; EC 1.15.1.1) were determined in leaves of different age and relative water content. The results indicate that different POD isoforms were stimulated during senescence and dehydration. Two of the soluble POD isoforms were anionic with pI 4.5, and two were cationic with pI 9.3 and 9.0. The activity of ascorbate peroxidase remained unchanged either by drought or senescence. For the first time, SOD isoforms have now been determined in this resurrection plant. Several SOD isoforms, all of the Mn type, were found to be anionic with pI 4 and a few others had pI from 5 to 6, while one band of FeSOD with a lower molecular weight was neutral. Rehydration brought about a remarkable decrease over the first hour in the activity of all the antioxidant enzymes examined but activity recovered 1 d after rehydration. The results confirmed that dehydration and senescence caused disturbance in the redox homeostasis of Ramonda leaves, while inducing different POD isoforms. A physiological role of peroxidase reaction with hydroxycinnamic acids in conservation and protection of cellular constituents of desiccated Ramonda leaves is suggested.
PB  - Oxford Univ Press, Oxford
T2  - Journal of Experimental Botany
T1  - Senescence- and drought-related changes in peroxidase and superoxide dismutase isoforms in leaves of Ramonda serbica
EP  - 1768
IS  - 8
SP  - 1759
VL  - 57
DO  - 10.1093/jxb/erl007
ER  - 

@article{
author = "Veljović-Jovanović, Sonja and Kukavica, Biljana and Stevanović, Branka and Navari-Izzo, Flavia",
year = "2006",
abstract = "Ramonda sp. (Gesneriaceae) is an endemic and relic plant in a very small group of poikilohydric angiosperms that are able to survive in an almost completely dehydrated state. Senescence- and drought-related changes in the activity of peroxidase (POD; EC 1.11.1.7), ascorbate peroxidase (EC 1.11.1.11), and superoxide dismutase (SOD; EC 1.15.1.1) were determined in leaves of different age and relative water content. The results indicate that different POD isoforms were stimulated during senescence and dehydration. Two of the soluble POD isoforms were anionic with pI 4.5, and two were cationic with pI 9.3 and 9.0. The activity of ascorbate peroxidase remained unchanged either by drought or senescence. For the first time, SOD isoforms have now been determined in this resurrection plant. Several SOD isoforms, all of the Mn type, were found to be anionic with pI 4 and a few others had pI from 5 to 6, while one band of FeSOD with a lower molecular weight was neutral. Rehydration brought about a remarkable decrease over the first hour in the activity of all the antioxidant enzymes examined but activity recovered 1 d after rehydration. The results confirmed that dehydration and senescence caused disturbance in the redox homeostasis of Ramonda leaves, while inducing different POD isoforms. A physiological role of peroxidase reaction with hydroxycinnamic acids in conservation and protection of cellular constituents of desiccated Ramonda leaves is suggested.",
publisher = "Oxford Univ Press, Oxford",
journal = "Journal of Experimental Botany",
title = "Senescence- and drought-related changes in peroxidase and superoxide dismutase isoforms in leaves of Ramonda serbica",
pages = "1768-1759",
number = "8",
volume = "57",
doi = "10.1093/jxb/erl007"
}

Veljović-Jovanović, S., Kukavica, B., Stevanović, B.,& Navari-Izzo, F.. (2006). Senescence- and drought-related changes in peroxidase and superoxide dismutase isoforms in leaves of Ramonda serbica. in Journal of Experimental Botany
Oxford Univ Press, Oxford., 57(8), 1759-1768.
https://doi.org/10.1093/jxb/erl007

Veljović-Jovanović S, Kukavica B, Stevanović B, Navari-Izzo F. Senescence- and drought-related changes in peroxidase and superoxide dismutase isoforms in leaves of Ramonda serbica. in Journal of Experimental Botany. 2006;57(8):1759-1768.
doi:10.1093/jxb/erl007 .

Veljović-Jovanović, Sonja, Kukavica, Biljana, Stevanović, Branka, Navari-Izzo, Flavia, "Senescence- and drought-related changes in peroxidase and superoxide dismutase isoforms in leaves of Ramonda serbica" in Journal of Experimental Botany, 57, no. 8 (2006):1759-1768,
https://doi.org/10.1093/jxb/erl007 . .