TY  - JOUR
AU  - Bulajic, Dragica V.
AU  - Drljaca, Jovana
AU  - Capo, Ivan
AU  - Savić, Slavica M.
AU  - Vojisavljević, Katarina
AU  - Hodzic, Aden
AU  - Sekulic, Slobodan
AU  - Bajkin, Branislav V.
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1474
AB  - In the present work, a biomaterial (SBA-16/HA) based on the growth of hydroxyapatite (HA) particles within an organized silica structure SBA-16 (Santa Barbara Amorphous-16) was developed to evaluate its application to act as a porous microenvironment promoting attachment and viability of human dental pulp stem cells of healthy deciduous teeth (SHED). First, SHED were isolated and their phenotypes were evaluated by flow cytometry. The samples of SBA-16/HA were characterized by X-ray diffraction (XRD), small and wide angle X-ray scattering (SWAXS), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) equipped with energy dispersive spectra detector (EDS). Afterward, cells were cultured in the eluates of the above-mentioned biomaterial aged for 24 hr, 7. and 14 days. Bio-Oss (R) and dentin particles are involved for comparison and cells are cultured in the eluates of these two materials also. Thiazolyl Blue Tetrazolium bromide assay-MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide assay) was used for the determination of cell viability. The results obtained by all aforementioned characterization methods of SBA-16/HA, revealed a uniform spherical mesoporous structure, an intrinsic characteristic of this material. This material displayed excellent biocompatibility on SHEDs, and even proliferative potential, indicating that SBA-16/HA could potentially serve as a suitable substrate for bone regeneration. Contrary to SBA-16/HA, dentin particles showed low cytotoxicity at all time points, compared to control and Bio-Oss (R) groups. Our results substantiate the idea that SBA-16/HA has a beneficial effect on SHEDs, thus paving the way toward developing new material for bone replacement.
PB  - Wiley, Hoboken
T2  - Microscopy Research and Technique
T1  - Biocompatibility of mesoporous SBA-16/hydroxyapatite nanocomposite and dentin demineralized particles on human dental pulp stem cells
DO  - 10.1002/jemt.24017
ER  - 

@article{
author = "Bulajic, Dragica V. and Drljaca, Jovana and Capo, Ivan and Savić, Slavica M. and Vojisavljević, Katarina and Hodzic, Aden and Sekulic, Slobodan and Bajkin, Branislav V.",
year = "2021",
abstract = "In the present work, a biomaterial (SBA-16/HA) based on the growth of hydroxyapatite (HA) particles within an organized silica structure SBA-16 (Santa Barbara Amorphous-16) was developed to evaluate its application to act as a porous microenvironment promoting attachment and viability of human dental pulp stem cells of healthy deciduous teeth (SHED). First, SHED were isolated and their phenotypes were evaluated by flow cytometry. The samples of SBA-16/HA were characterized by X-ray diffraction (XRD), small and wide angle X-ray scattering (SWAXS), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) equipped with energy dispersive spectra detector (EDS). Afterward, cells were cultured in the eluates of the above-mentioned biomaterial aged for 24 hr, 7. and 14 days. Bio-Oss (R) and dentin particles are involved for comparison and cells are cultured in the eluates of these two materials also. Thiazolyl Blue Tetrazolium bromide assay-MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide assay) was used for the determination of cell viability. The results obtained by all aforementioned characterization methods of SBA-16/HA, revealed a uniform spherical mesoporous structure, an intrinsic characteristic of this material. This material displayed excellent biocompatibility on SHEDs, and even proliferative potential, indicating that SBA-16/HA could potentially serve as a suitable substrate for bone regeneration. Contrary to SBA-16/HA, dentin particles showed low cytotoxicity at all time points, compared to control and Bio-Oss (R) groups. Our results substantiate the idea that SBA-16/HA has a beneficial effect on SHEDs, thus paving the way toward developing new material for bone replacement.",
publisher = "Wiley, Hoboken",
journal = "Microscopy Research and Technique",
title = "Biocompatibility of mesoporous SBA-16/hydroxyapatite nanocomposite and dentin demineralized particles on human dental pulp stem cells",
doi = "10.1002/jemt.24017"
}

Bulajic, D. V., Drljaca, J., Capo, I., Savić, S. M., Vojisavljević, K., Hodzic, A., Sekulic, S.,& Bajkin, B. V.. (2021). Biocompatibility of mesoporous SBA-16/hydroxyapatite nanocomposite and dentin demineralized particles on human dental pulp stem cells. in Microscopy Research and Technique
Wiley, Hoboken..
https://doi.org/10.1002/jemt.24017

Bulajic DV, Drljaca J, Capo I, Savić SM, Vojisavljević K, Hodzic A, Sekulic S, Bajkin BV. Biocompatibility of mesoporous SBA-16/hydroxyapatite nanocomposite and dentin demineralized particles on human dental pulp stem cells. in Microscopy Research and Technique. 2021;.
doi:10.1002/jemt.24017 .

Bulajic, Dragica V., Drljaca, Jovana, Capo, Ivan, Savić, Slavica M., Vojisavljević, Katarina, Hodzic, Aden, Sekulic, Slobodan, Bajkin, Branislav V., "Biocompatibility of mesoporous SBA-16/hydroxyapatite nanocomposite and dentin demineralized particles on human dental pulp stem cells" in Microscopy Research and Technique (2021),
https://doi.org/10.1002/jemt.24017 . .

TY  - JOUR
AU  - Stojadinović, Gordana
AU  - Martać, Ljiljana
AU  - Podgorac, Jelena
AU  - Spasić, Slađana
AU  - Petković, Branka
AU  - Sekulic, Slobodan
AU  - Kesic, Srdjan
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1386
AB  - A detailed analysis of the anesthetic-induced modulation of intracerebellar electrical activity is an important step to understand the functional brain responses to anesthesia. We examined the electrical activity recorded from different cortical layers: molecular layer (ML), Purkinje cell layer (PCL), granular layer (GL) and the white matter (WM) in the vermian part of rat cerebellar lobule V during Nembutal anesthesia using spectral and fractal analysis. Spectral analysis revealed a difference in the mean relative power of delta (0.1-4.0 Hz) and theta (4.1-8.0 Hz) frequencies through the cerebellar layers. Compared to the ML, delta activity increased significantly in the GL, while theta activity decreased in the GL and the WM. Fractal analysis revealed that the mean value of Higuchi's fractal dimension (HFD) increased, starting from the ML to the WM. Theta activity exhibited a negative correlation with the HFD value in the ML. In contrast, the gamma activity showed a positive correlation with the HFD value in the ML and the GL. The combined use of spectral and fractal analyses revealed that Nembutal displays different effects on rat cerebellar electrical activity, which largely depends on the neurochemical and electrophysiological organization of the cerebellar layers.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - The effects of Nembutal on the intracerebellar EEG activity revealed by spectral and fractal analysis
EP  - 432
IS  - 3
SP  - 425
VL  - 72
DO  - 10.2298/ABS200524036S
ER  - 

@article{
author = "Stojadinović, Gordana and Martać, Ljiljana and Podgorac, Jelena and Spasić, Slađana and Petković, Branka and Sekulic, Slobodan and Kesic, Srdjan",
year = "2020",
abstract = "A detailed analysis of the anesthetic-induced modulation of intracerebellar electrical activity is an important step to understand the functional brain responses to anesthesia. We examined the electrical activity recorded from different cortical layers: molecular layer (ML), Purkinje cell layer (PCL), granular layer (GL) and the white matter (WM) in the vermian part of rat cerebellar lobule V during Nembutal anesthesia using spectral and fractal analysis. Spectral analysis revealed a difference in the mean relative power of delta (0.1-4.0 Hz) and theta (4.1-8.0 Hz) frequencies through the cerebellar layers. Compared to the ML, delta activity increased significantly in the GL, while theta activity decreased in the GL and the WM. Fractal analysis revealed that the mean value of Higuchi's fractal dimension (HFD) increased, starting from the ML to the WM. Theta activity exhibited a negative correlation with the HFD value in the ML. In contrast, the gamma activity showed a positive correlation with the HFD value in the ML and the GL. The combined use of spectral and fractal analyses revealed that Nembutal displays different effects on rat cerebellar electrical activity, which largely depends on the neurochemical and electrophysiological organization of the cerebellar layers.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "The effects of Nembutal on the intracerebellar EEG activity revealed by spectral and fractal analysis",
pages = "432-425",
number = "3",
volume = "72",
doi = "10.2298/ABS200524036S"
}

Stojadinović, G., Martać, L., Podgorac, J., Spasić, S., Petković, B., Sekulic, S.,& Kesic, S.. (2020). The effects of Nembutal on the intracerebellar EEG activity revealed by spectral and fractal analysis. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 72(3), 425-432.
https://doi.org/10.2298/ABS200524036S

Stojadinović G, Martać L, Podgorac J, Spasić S, Petković B, Sekulic S, Kesic S. The effects of Nembutal on the intracerebellar EEG activity revealed by spectral and fractal analysis. in Archives of Biological Sciences. 2020;72(3):425-432.
doi:10.2298/ABS200524036S .

Stojadinović, Gordana, Martać, Ljiljana, Podgorac, Jelena, Spasić, Slađana, Petković, Branka, Sekulic, Slobodan, Kesic, Srdjan, "The effects of Nembutal on the intracerebellar EEG activity revealed by spectral and fractal analysis" in Archives of Biological Sciences, 72, no. 3 (2020):425-432,
https://doi.org/10.2298/ABS200524036S . .

TY  - JOUR
AU  - Spasić, Slađana
AU  - Culic, Milka
AU  - Grbic, Gordana
AU  - Martać, Ljiljana
AU  - Sekulic, Slobodan
AU  - Mutavdžić, Dragosav
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/253
AB  - The cerebellum, even when not directly damaged, is potentially interesting for understanding the adaptive responses to brain injury. Cerebellar electrocortical activity (ECoG) in rats was studied using spectral and fractal analysis after single and repeated unilateral injury of the parietal cortex. Local field potentials of cerebellar paravermal cortex were recorded before brain injury, in the acute phase (up to 2.5 hours) after a first injury of anesthetized rats, and then before and after second, third, and, in some cases, fourth injury. Relative gamma power (32.1-128.0 Hz) and fractal dimension of ECoGs were temporarily increased after the first injury. However, there was a permanent mild increase in gamma activity and a mild increase in the fractal dimension of cerebellar activity as a chronic change after repeated remote brain injury. There was a negative linear correlation between the normalized difference in fractal dimensions and normalized difference in gamma powers of cerebellar activity only in the case of repeated brain injury. This is the first study showing that correlation between the parameters of spectral and fractal analyses of cerebellar activity can discriminate between single and repeated brain injuries, and is, therefore, a promising approach for identifying specific pathophysiological states.
PB  - Springer, New York
T2  - Bulletin of Mathematical Biology
T1  - Spectral and fractal analysis of cerebellar activity after single and repeated brain injury
EP  - 1249
IS  - 4
SP  - 1235
VL  - 70
DO  - 10.1007/s11538-008-9306-5
ER  - 

@article{
author = "Spasić, Slađana and Culic, Milka and Grbic, Gordana and Martać, Ljiljana and Sekulic, Slobodan and Mutavdžić, Dragosav",
year = "2008",
abstract = "The cerebellum, even when not directly damaged, is potentially interesting for understanding the adaptive responses to brain injury. Cerebellar electrocortical activity (ECoG) in rats was studied using spectral and fractal analysis after single and repeated unilateral injury of the parietal cortex. Local field potentials of cerebellar paravermal cortex were recorded before brain injury, in the acute phase (up to 2.5 hours) after a first injury of anesthetized rats, and then before and after second, third, and, in some cases, fourth injury. Relative gamma power (32.1-128.0 Hz) and fractal dimension of ECoGs were temporarily increased after the first injury. However, there was a permanent mild increase in gamma activity and a mild increase in the fractal dimension of cerebellar activity as a chronic change after repeated remote brain injury. There was a negative linear correlation between the normalized difference in fractal dimensions and normalized difference in gamma powers of cerebellar activity only in the case of repeated brain injury. This is the first study showing that correlation between the parameters of spectral and fractal analyses of cerebellar activity can discriminate between single and repeated brain injuries, and is, therefore, a promising approach for identifying specific pathophysiological states.",
publisher = "Springer, New York",
journal = "Bulletin of Mathematical Biology",
title = "Spectral and fractal analysis of cerebellar activity after single and repeated brain injury",
pages = "1249-1235",
number = "4",
volume = "70",
doi = "10.1007/s11538-008-9306-5"
}

Spasić, S., Culic, M., Grbic, G., Martać, L., Sekulic, S.,& Mutavdžić, D.. (2008). Spectral and fractal analysis of cerebellar activity after single and repeated brain injury. in Bulletin of Mathematical Biology
Springer, New York., 70(4), 1235-1249.
https://doi.org/10.1007/s11538-008-9306-5

Spasić S, Culic M, Grbic G, Martać L, Sekulic S, Mutavdžić D. Spectral and fractal analysis of cerebellar activity after single and repeated brain injury. in Bulletin of Mathematical Biology. 2008;70(4):1235-1249.
doi:10.1007/s11538-008-9306-5 .

Spasić, Slađana, Culic, Milka, Grbic, Gordana, Martać, Ljiljana, Sekulic, Slobodan, Mutavdžić, Dragosav, "Spectral and fractal analysis of cerebellar activity after single and repeated brain injury" in Bulletin of Mathematical Biology, 70, no. 4 (2008):1235-1249,
https://doi.org/10.1007/s11538-008-9306-5 . .