TY  - JOUR
AU  - Veljović-Jovanović, Sonja
AU  - kasalica, becko
AU  - Miletić, Katarina
AU  - Vidović, Marija
AU  - Šušić, Nikola
AU  - Jeremić, Dejan
AU  - Belča, Ivan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2363
AB  - Chloroplast movement rapidly ameliorates the effects of suboptimal light intensity by accumulating along the periclinal cell walls, as well as the effects of excess light by shifting to the anticlinal cell walls. These acclimation responses are triggered by phototropins located at the plasma membrane and chloroplast envelope. Here, we used a recently developed non-invasive system sensitive to very small changes in red light leaf transmittance to perform long-term continuous measurements of dark–light transitions. As a model system, we used variegated Pelargonium zonale leaves containing green sectors (GS) with fully developed chloroplasts and achlorophyllous, white sectors (WS) with undifferentiated plastids, and higher phototropin expression levels. We observed biphasic changes in the red-light transmittance and oscillations triggered by medium intensities of white light, described by a transient peak preceded by a constant decrease in transmittance level. A slight change in red-light transmittance was recorded even in WS. Furthermore, the chloroplast position at lower light intensities affected the rapid light curves, while high light intensity decreased saturated electron transport, maximum quantum efficiency of photosystem II, and increased non-photochemical quenching of chlorophyll fluorescence and epidermal flavonoids. Our results extend the knowledge of light-dependent chloroplast movements and thus contribute to a better understanding of their role in regulating photosynthesis under fluctuating light conditions.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Red-Light Transmittance Changes in Variegated Pelargonium zonale—Diurnal Variation in Chloroplast Movement and Photosystem II Efficiency
EP  - 16
IS  - 18
SP  - 1
VL  - 24
DO  - 10.3390/ijms241814265
ER  - 

@article{
author = "Veljović-Jovanović, Sonja and kasalica, becko and Miletić, Katarina and Vidović, Marija and Šušić, Nikola and Jeremić, Dejan and Belča, Ivan",
year = "2023",
abstract = "Chloroplast movement rapidly ameliorates the effects of suboptimal light intensity by accumulating along the periclinal cell walls, as well as the effects of excess light by shifting to the anticlinal cell walls. These acclimation responses are triggered by phototropins located at the plasma membrane and chloroplast envelope. Here, we used a recently developed non-invasive system sensitive to very small changes in red light leaf transmittance to perform long-term continuous measurements of dark–light transitions. As a model system, we used variegated Pelargonium zonale leaves containing green sectors (GS) with fully developed chloroplasts and achlorophyllous, white sectors (WS) with undifferentiated plastids, and higher phototropin expression levels. We observed biphasic changes in the red-light transmittance and oscillations triggered by medium intensities of white light, described by a transient peak preceded by a constant decrease in transmittance level. A slight change in red-light transmittance was recorded even in WS. Furthermore, the chloroplast position at lower light intensities affected the rapid light curves, while high light intensity decreased saturated electron transport, maximum quantum efficiency of photosystem II, and increased non-photochemical quenching of chlorophyll fluorescence and epidermal flavonoids. Our results extend the knowledge of light-dependent chloroplast movements and thus contribute to a better understanding of their role in regulating photosynthesis under fluctuating light conditions.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Red-Light Transmittance Changes in Variegated Pelargonium zonale—Diurnal Variation in Chloroplast Movement and Photosystem II Efficiency",
pages = "16-1",
number = "18",
volume = "24",
doi = "10.3390/ijms241814265"
}

Veljović-Jovanović, S., kasalica, b., Miletić, K., Vidović, M., Šušić, N., Jeremić, D.,& Belča, I.. (2023). Red-Light Transmittance Changes in Variegated Pelargonium zonale—Diurnal Variation in Chloroplast Movement and Photosystem II Efficiency. in International Journal of Molecular Sciences
MDPI., 24(18), 1-16.
https://doi.org/10.3390/ijms241814265

Veljović-Jovanović S, kasalica B, Miletić K, Vidović M, Šušić N, Jeremić D, Belča I. Red-Light Transmittance Changes in Variegated Pelargonium zonale—Diurnal Variation in Chloroplast Movement and Photosystem II Efficiency. in International Journal of Molecular Sciences. 2023;24(18):1-16.
doi:10.3390/ijms241814265 .

Veljović-Jovanović, Sonja, kasalica, becko, Miletić, Katarina, Vidović, Marija, Šušić, Nikola, Jeremić, Dejan, Belča, Ivan, "Red-Light Transmittance Changes in Variegated Pelargonium zonale—Diurnal Variation in Chloroplast Movement and Photosystem II Efficiency" in International Journal of Molecular Sciences, 24, no. 18 (2023):1-16,
https://doi.org/10.3390/ijms241814265 . .

TY  - JOUR
AU  - Milić Komić, Sonja
AU  - Živanović, Bojana
AU  - Dumanović, Jelena
AU  - kolarž, Predrag
AU  - Sedlarević Zorić, Ana
AU  - Morina, Filis
AU  - Vidović, Marija
AU  - Veljović-Jovanović, Sonja
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2220
AB  - Three basil plant varieties (Ocimum basilicum var. Genovese, Ocimum x citriodorum, and Ocimum
basilicum var. purpurascens) were grown under moderate light (about 300 umol photons m-2 s-1)
in a glasshouse or growth chamber and then either transferred to an open field (average daily dose:
29.2 kJ m-2 d-1) or additionally exposed to UV-B irradiation in a growth chamber (29.16 kJ m-2 d-1),
to reveal the variety-specific and light-specific acclimation responses. Total antioxidant capacity
(TAC), phenolic profile, ascorbate content, and class III peroxidase (POD) activity were used to determine
the antioxidant status of leaves under all four light regimes. Exposure to high solar irradiation
at the open field resulted in an increase in TAC, total hydroxycinnamic acids (HCAs, especially
caffeic acid), flavonoids, and epidermal UV-absorbing substances in all three varieties, as well as a
two-fold increase in the leaf dry/fresh weight ratio. The supplemental UV-B irradiation induced
preferential accumulation of HCAs (rosmarinic acid) over flavonoids, increased TAC and POD activity,
but decreased the ascorbate content in the leaves, and inhibited the accumulation of epidermal
flavonoids in all basil varieties. Furthermore, characteristic leaf curling and UV-B-induced inhibition
of plant growth were observed in all basil varieties, while a pro-oxidant effect of UV-B was indicated
with H2O2 accumulation in the leaves and spotty leaf browning. The extent of these morphological
changes, and oxidative damage depended on the basil cultivar, implies a genotype-specific tolerance
mechanism to high doses of UV-B irradiation.
PB  - MDPI AG, POSTFACH, BASEL, SWITZERLAND, CH-4005
T2  - International Journal of Molecular Sciences
T1  - Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties
VL  - 24(20), 15350
DO  - 10.3390/ijms242015350
ER  - 

@article{
author = "Milić Komić, Sonja and Živanović, Bojana and Dumanović, Jelena and kolarž, Predrag and Sedlarević Zorić, Ana and Morina, Filis and Vidović, Marija and Veljović-Jovanović, Sonja",
year = "2023",
abstract = "Three basil plant varieties (Ocimum basilicum var. Genovese, Ocimum x citriodorum, and Ocimum
basilicum var. purpurascens) were grown under moderate light (about 300 umol photons m-2 s-1)
in a glasshouse or growth chamber and then either transferred to an open field (average daily dose:
29.2 kJ m-2 d-1) or additionally exposed to UV-B irradiation in a growth chamber (29.16 kJ m-2 d-1),
to reveal the variety-specific and light-specific acclimation responses. Total antioxidant capacity
(TAC), phenolic profile, ascorbate content, and class III peroxidase (POD) activity were used to determine
the antioxidant status of leaves under all four light regimes. Exposure to high solar irradiation
at the open field resulted in an increase in TAC, total hydroxycinnamic acids (HCAs, especially
caffeic acid), flavonoids, and epidermal UV-absorbing substances in all three varieties, as well as a
two-fold increase in the leaf dry/fresh weight ratio. The supplemental UV-B irradiation induced
preferential accumulation of HCAs (rosmarinic acid) over flavonoids, increased TAC and POD activity,
but decreased the ascorbate content in the leaves, and inhibited the accumulation of epidermal
flavonoids in all basil varieties. Furthermore, characteristic leaf curling and UV-B-induced inhibition
of plant growth were observed in all basil varieties, while a pro-oxidant effect of UV-B was indicated
with H2O2 accumulation in the leaves and spotty leaf browning. The extent of these morphological
changes, and oxidative damage depended on the basil cultivar, implies a genotype-specific tolerance
mechanism to high doses of UV-B irradiation.",
publisher = "MDPI AG, POSTFACH, BASEL, SWITZERLAND, CH-4005",
journal = "International Journal of Molecular Sciences",
title = "Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties",
volume = "24(20), 15350",
doi = "10.3390/ijms242015350"
}

Milić Komić, S., Živanović, B., Dumanović, J., kolarž, P., Sedlarević Zorić, A., Morina, F., Vidović, M.,& Veljović-Jovanović, S.. (2023). Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties. in International Journal of Molecular Sciences
MDPI AG, POSTFACH, BASEL, SWITZERLAND, CH-4005., 24(20), 15350.
https://doi.org/10.3390/ijms242015350

Milić Komić S, Živanović B, Dumanović J, kolarž P, Sedlarević Zorić A, Morina F, Vidović M, Veljović-Jovanović S. Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties. in International Journal of Molecular Sciences. 2023;24(20), 15350.
doi:10.3390/ijms242015350 .

Milić Komić, Sonja, Živanović, Bojana, Dumanović, Jelena, kolarž, Predrag, Sedlarević Zorić, Ana, Morina, Filis, Vidović, Marija, Veljović-Jovanović, Sonja, "Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties" in International Journal of Molecular Sciences, 24(20), 15350 (2023),
https://doi.org/10.3390/ijms242015350 . .

TY  - CONF
AU  - Pantelic, Ana
AU  - Stevanovic, Strahinja
AU  - Milić Komić, Sonja
AU  - Kilibarda, Nataša
AU  - Vidović, Marija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3087
AB  - Ramonda serbica Panc. is an ancient resurrection plant, that survives a long desiccation period and fully recovers metabolic functions upon watering. The main characteristic of desiccationtolerant plant species is their ability to accumulate protective late embryogenesis abounded protein (LEAPs). To propose their role in R. serbica desiccation tolerance we structurally analysed LEAPs in hydrated and desiccated leaves. According to transcriptomics, 318 LEAPs were identified and classified into seven family groups based on protein BLAST analysis and conserved motifs (Pfam). The largest LEAPs belonged to the LEA2 and LEA4 protein family groups. We employed online tools to analyse physicochemical characteristics (Expasy, ProtParam, BioPython, GRAVY calculator), disorder propensity, and characterization protein structures (FELLS, JPred, SOPMA, PsiPred, Phyre2, Espritz-DisProt, Espritz-X, Iupred, TMHMM, +Heliquest). The most abundant, atypical LEA2 group containing 127, mostly hydrophobic proteins, was divided into five subgroups. Members of this group were predicted to fold into globular domains, β-barrel at the C-terminus, followed by transmembrane hydrophobic-helices and disordered N-terminal regions. Results indicated the possible involvement in the protection of the chloroplastic membranes. The LEA4 group exhibited an exceptionally high tendency to form amphipathic α-helices and simultaneously had a high disorder propensity. This group is made of 96 proteins, classified into 3 subgroups. The high content of polar and charged amino acids (lysine, glutamate, and aspartate) is characteristic of this group. Motifs corresponding to the R. serbica LEA4 protein family group folded into A-type α-helices that contained positive, negative, and hydrophobic surfaces. Based on previous knowledge, the possible functions of the LEA2 and LEA4 groups are discussed with significant implications on cell preservation technology and the improvement of crop drought tolerance.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference, 2023, 4, 68-68
T1  - Two contrasting late embryogenesis abounded protein family groups of Ramonda serbica Panc.
SP  - 68
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3087
ER  - 

@conference{
author = "Pantelic, Ana and Stevanovic, Strahinja and Milić Komić, Sonja and Kilibarda, Nataša and Vidović, Marija",
year = "2023",
abstract = "Ramonda serbica Panc. is an ancient resurrection plant, that survives a long desiccation period and fully recovers metabolic functions upon watering. The main characteristic of desiccationtolerant plant species is their ability to accumulate protective late embryogenesis abounded protein (LEAPs). To propose their role in R. serbica desiccation tolerance we structurally analysed LEAPs in hydrated and desiccated leaves. According to transcriptomics, 318 LEAPs were identified and classified into seven family groups based on protein BLAST analysis and conserved motifs (Pfam). The largest LEAPs belonged to the LEA2 and LEA4 protein family groups. We employed online tools to analyse physicochemical characteristics (Expasy, ProtParam, BioPython, GRAVY calculator), disorder propensity, and characterization protein structures (FELLS, JPred, SOPMA, PsiPred, Phyre2, Espritz-DisProt, Espritz-X, Iupred, TMHMM, +Heliquest). The most abundant, atypical LEA2 group containing 127, mostly hydrophobic proteins, was divided into five subgroups. Members of this group were predicted to fold into globular domains, β-barrel at the C-terminus, followed by transmembrane hydrophobic-helices and disordered N-terminal regions. Results indicated the possible involvement in the protection of the chloroplastic membranes. The LEA4 group exhibited an exceptionally high tendency to form amphipathic α-helices and simultaneously had a high disorder propensity. This group is made of 96 proteins, classified into 3 subgroups. The high content of polar and charged amino acids (lysine, glutamate, and aspartate) is characteristic of this group. Motifs corresponding to the R. serbica LEA4 protein family group folded into A-type α-helices that contained positive, negative, and hydrophobic surfaces. Based on previous knowledge, the possible functions of the LEA2 and LEA4 groups are discussed with significant implications on cell preservation technology and the improvement of crop drought tolerance.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference, 2023, 4, 68-68",
title = "Two contrasting late embryogenesis abounded protein family groups of Ramonda serbica Panc.",
pages = "68",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3087"
}

Pantelic, A., Stevanovic, S., Milić Komić, S., Kilibarda, N.,& Vidović, M.. (2023). Two contrasting late embryogenesis abounded protein family groups of Ramonda serbica Panc.. in 4th Belgrade Bioinformatics Conference, 2023, 4, 68-68
Belgrade : Institute of molecular genetics and genetic engineering., 68.
https://hdl.handle.net/21.15107/rcub_rimsi_3087

Pantelic A, Stevanovic S, Milić Komić S, Kilibarda N, Vidović M. Two contrasting late embryogenesis abounded protein family groups of Ramonda serbica Panc.. in 4th Belgrade Bioinformatics Conference, 2023, 4, 68-68. 2023;:68.
https://hdl.handle.net/21.15107/rcub_rimsi_3087 .

Pantelic, Ana, Stevanovic, Strahinja, Milić Komić, Sonja, Kilibarda, Nataša, Vidović, Marija, "Two contrasting late embryogenesis abounded protein family groups of Ramonda serbica Panc." in 4th Belgrade Bioinformatics Conference, 2023, 4, 68-68 (2023):68,
https://hdl.handle.net/21.15107/rcub_rimsi_3087 .

TY  - JOUR
AU  - Milić, Dejana
AU  - Živanović, Bojana
AU  - Samardžić, Jelena
AU  - Nikolić, Nenad
AU  - Cukier, Caroline
AU  - Limami, Anis M.
AU  - Vidović, Marija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2390
AB  - Plants are inevitably exposed to extreme climatic conditions that lead to a disturbed balance
between the amount of absorbed energy and their ability to process it. Variegated leaves with
photosynthetically active green leaf tissue (GL) and photosynthetically inactive white leaf tissue
(WL) are an excellent model system to study source–sink interactions within the same leaf under the
same microenvironmental conditions. We demonstrated that under excess excitation energy (EEE)
conditions (high irradiance and lower temperature), regulated metabolic reprogramming in both
leaf tissues allowed an increased consumption of reducing equivalents, as evidenced by preserved
maximum efficiency of photosystem II (φPSII) at the end of the experiment. GL of the EEE-treated
plants employed two strategies: (i) the accumulation of flavonoid glycosides, especially cyanidin
glycosides, as an alternative electron sink, and (ii) cell wall stiffening by cellulose, pectin, and lignin
accumulation. On the other hand, WL increased the amount of free amino acids, mainly arginine,
asparagine, branched-chain and aromatic amino acids, as well as kaempferol and quercetin glycosides.
Thus, WL acts as an important energy escape valve that is required in order to maintain the successful
performance of the GL sectors under EEE conditions. Finally, this role could be an adaptive value of
variegation, as no consistent conclusions about its ecological benefits have been proposed so far.
PB  - MDPI AG, POSTFACH, BASEL, SWITZERLAND, CH-4005
T2  - International Journal of Molecular Sciences
T1  - Carbon and Nitrogen Allocation between the Sink and Source Leaf Tissue in Response to the Excess Excitation Energy Conditions
EP  - 2269
IS  - 3
SP  - 2269
VL  - 24
DO  - 10.3390/ijms24032269
ER  - 

@article{
author = "Milić, Dejana and Živanović, Bojana and Samardžić, Jelena and Nikolić, Nenad and Cukier, Caroline and Limami, Anis M. and Vidović, Marija",
year = "2023",
abstract = "Plants are inevitably exposed to extreme climatic conditions that lead to a disturbed balance
between the amount of absorbed energy and their ability to process it. Variegated leaves with
photosynthetically active green leaf tissue (GL) and photosynthetically inactive white leaf tissue
(WL) are an excellent model system to study source–sink interactions within the same leaf under the
same microenvironmental conditions. We demonstrated that under excess excitation energy (EEE)
conditions (high irradiance and lower temperature), regulated metabolic reprogramming in both
leaf tissues allowed an increased consumption of reducing equivalents, as evidenced by preserved
maximum efficiency of photosystem II (φPSII) at the end of the experiment. GL of the EEE-treated
plants employed two strategies: (i) the accumulation of flavonoid glycosides, especially cyanidin
glycosides, as an alternative electron sink, and (ii) cell wall stiffening by cellulose, pectin, and lignin
accumulation. On the other hand, WL increased the amount of free amino acids, mainly arginine,
asparagine, branched-chain and aromatic amino acids, as well as kaempferol and quercetin glycosides.
Thus, WL acts as an important energy escape valve that is required in order to maintain the successful
performance of the GL sectors under EEE conditions. Finally, this role could be an adaptive value of
variegation, as no consistent conclusions about its ecological benefits have been proposed so far.",
publisher = "MDPI AG, POSTFACH, BASEL, SWITZERLAND, CH-4005",
journal = "International Journal of Molecular Sciences",
title = "Carbon and Nitrogen Allocation between the Sink and Source Leaf Tissue in Response to the Excess Excitation Energy Conditions",
pages = "2269-2269",
number = "3",
volume = "24",
doi = "10.3390/ijms24032269"
}

Milić, D., Živanović, B., Samardžić, J., Nikolić, N., Cukier, C., Limami, A. M.,& Vidović, M.. (2023). Carbon and Nitrogen Allocation between the Sink and Source Leaf Tissue in Response to the Excess Excitation Energy Conditions. in International Journal of Molecular Sciences
MDPI AG, POSTFACH, BASEL, SWITZERLAND, CH-4005., 24(3), 2269-2269.
https://doi.org/10.3390/ijms24032269

Milić D, Živanović B, Samardžić J, Nikolić N, Cukier C, Limami AM, Vidović M. Carbon and Nitrogen Allocation between the Sink and Source Leaf Tissue in Response to the Excess Excitation Energy Conditions. in International Journal of Molecular Sciences. 2023;24(3):2269-2269.
doi:10.3390/ijms24032269 .

Milić, Dejana, Živanović, Bojana, Samardžić, Jelena, Nikolić, Nenad, Cukier, Caroline, Limami, Anis M., Vidović, Marija, "Carbon and Nitrogen Allocation between the Sink and Source Leaf Tissue in Response to the Excess Excitation Energy Conditions" in International Journal of Molecular Sciences, 24, no. 3 (2023):2269-2269,
https://doi.org/10.3390/ijms24032269 . .

TY  - JOUR
AU  - Milić Komić, Sonja
AU  - Veljović-Jovanović, Sonja
AU  - Pantelic, Ana
AU  - Vidović, Marija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2900
AB  - Ressurection plants are extraordinary because of their ability to withstand long periods without water, enter a state of anhydrobiosis, and fully recover upon water arrival. Ramonda serbica Panč. is a relic and endemic species that belongs to a very small group of desiccation-tolerant plants in Europe. Underlying physiological, molecular and morphological mechanisms that enable these plants to survive harsh environmental conditions have been an appealing subject of many researchers. Most of the genes responsible for this amazing ability are present in other plants, and research of those genes which could be activated in crops is growing much more attention because of the imminent crisis regarding food supplies in the near future. Key components involved in the response to dehydration in R. serbica plants were analysed through a comprehensive transcriptomic, proteomic, metabolite and photosynthetic study. Late embryogenesis abundant proteins play a significant role in the complex defence processes involved in desiccation tolerance. Defining LEAPs physicochemical characteristics and specific physiological functions may lead us to their applicability in other areas of research.
PB  - Novi Sad : Faculty of Sciences, Department of Biology
T2  - Biologia Serbica
T1  - Structural characterisation of late embryogenesis abundant proteins in Ramonda serbica Panč.
EP  - 66
IS  - 44
SP  - 59
VL  - 1
DO  - 10.5281/zenodo.7075212
ER  - 

@article{
author = "Milić Komić, Sonja and Veljović-Jovanović, Sonja and Pantelic, Ana and Vidović, Marija",
year = "2022",
abstract = "Ressurection plants are extraordinary because of their ability to withstand long periods without water, enter a state of anhydrobiosis, and fully recover upon water arrival. Ramonda serbica Panč. is a relic and endemic species that belongs to a very small group of desiccation-tolerant plants in Europe. Underlying physiological, molecular and morphological mechanisms that enable these plants to survive harsh environmental conditions have been an appealing subject of many researchers. Most of the genes responsible for this amazing ability are present in other plants, and research of those genes which could be activated in crops is growing much more attention because of the imminent crisis regarding food supplies in the near future. Key components involved in the response to dehydration in R. serbica plants were analysed through a comprehensive transcriptomic, proteomic, metabolite and photosynthetic study. Late embryogenesis abundant proteins play a significant role in the complex defence processes involved in desiccation tolerance. Defining LEAPs physicochemical characteristics and specific physiological functions may lead us to their applicability in other areas of research.",
publisher = "Novi Sad : Faculty of Sciences, Department of Biology",
journal = "Biologia Serbica",
title = "Structural characterisation of late embryogenesis abundant proteins in Ramonda serbica Panč.",
pages = "66-59",
number = "44",
volume = "1",
doi = "10.5281/zenodo.7075212"
}

Milić Komić, S., Veljović-Jovanović, S., Pantelic, A.,& Vidović, M.. (2022). Structural characterisation of late embryogenesis abundant proteins in Ramonda serbica Panč.. in Biologia Serbica
Novi Sad : Faculty of Sciences, Department of Biology., 1(44), 59-66.
https://doi.org/10.5281/zenodo.7075212

Milić Komić S, Veljović-Jovanović S, Pantelic A, Vidović M. Structural characterisation of late embryogenesis abundant proteins in Ramonda serbica Panč.. in Biologia Serbica. 2022;1(44):59-66.
doi:10.5281/zenodo.7075212 .

Milić Komić, Sonja, Veljović-Jovanović, Sonja, Pantelic, Ana, Vidović, Marija, "Structural characterisation of late embryogenesis abundant proteins in Ramonda serbica Panč." in Biologia Serbica, 1, no. 44 (2022):59-66,
https://doi.org/10.5281/zenodo.7075212 . .

TY  - JOUR
AU  - Vidović, Marija
AU  - Battisti, Ilaria
AU  - Pantelić, Ana
AU  - Morina, Filis
AU  - Arrigoni, Giorgio
AU  - Masi, Antonio
AU  - Veljović-Jovanović, Sonja
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1574
AB  - The resurrection plant Ramonda serbica Panc. survives long desiccation periods and fully recovers metabolic functions within one day upon watering. This study aimed to identify key candidates and pathways involved in desiccation tolerance in R. serbica. We combined differential transcriptomics and proteomics, phenolic and sugar analysis, FTIR analysis of the cell wall polymers, and detailed analysis of the photosynthetic electron transport (PET) chain. The proteomic analysis allowed the relative quantification of 1192 different protein groups, of which 408 were differentially abundant between hydrated (HL) and desiccated leaves (DL). Almost all differentially abundant proteins related to photosynthetic processes were less abundant, while chlorophyll fluorescence measurements implied shifting from linear PET to cyclic electron transport (CET). The levels of H2O2 scavenging enzymes, ascorbate-glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn superoxide dismutase (SOD) were reduced in DL. However, six germin-like proteins (GLPs), four Cu/ZnSOD isoforms, three polyphenol oxidases, and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins), were desiccation-inducible. Desiccation provoked cell wall remodeling related to GLP-derived H2O2/HO● activity and pectin demethylesterification. This comprehensive study contributes to understanding the role and regulation of the main metabolic pathways during desiccation aiming at crop drought tolerance improvement
PB  - MDPI
T2  - Plants
T1  - Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study
IS  - 9
SP  - 1199
VL  - 11
DO  - 10.3390/plants11091199
ER  - 

@article{
author = "Vidović, Marija and Battisti, Ilaria and Pantelić, Ana and Morina, Filis and Arrigoni, Giorgio and Masi, Antonio and Veljović-Jovanović, Sonja",
year = "2022",
abstract = "The resurrection plant Ramonda serbica Panc. survives long desiccation periods and fully recovers metabolic functions within one day upon watering. This study aimed to identify key candidates and pathways involved in desiccation tolerance in R. serbica. We combined differential transcriptomics and proteomics, phenolic and sugar analysis, FTIR analysis of the cell wall polymers, and detailed analysis of the photosynthetic electron transport (PET) chain. The proteomic analysis allowed the relative quantification of 1192 different protein groups, of which 408 were differentially abundant between hydrated (HL) and desiccated leaves (DL). Almost all differentially abundant proteins related to photosynthetic processes were less abundant, while chlorophyll fluorescence measurements implied shifting from linear PET to cyclic electron transport (CET). The levels of H2O2 scavenging enzymes, ascorbate-glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn superoxide dismutase (SOD) were reduced in DL. However, six germin-like proteins (GLPs), four Cu/ZnSOD isoforms, three polyphenol oxidases, and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins), were desiccation-inducible. Desiccation provoked cell wall remodeling related to GLP-derived H2O2/HO● activity and pectin demethylesterification. This comprehensive study contributes to understanding the role and regulation of the main metabolic pathways during desiccation aiming at crop drought tolerance improvement",
publisher = "MDPI",
journal = "Plants",
title = "Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study",
number = "9",
pages = "1199",
volume = "11",
doi = "10.3390/plants11091199"
}

Vidović, M., Battisti, I., Pantelić, A., Morina, F., Arrigoni, G., Masi, A.,& Veljović-Jovanović, S.. (2022). Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study. in Plants
MDPI., 11(9), 1199.
https://doi.org/10.3390/plants11091199

Vidović M, Battisti I, Pantelić A, Morina F, Arrigoni G, Masi A, Veljović-Jovanović S. Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study. in Plants. 2022;11(9):1199.
doi:10.3390/plants11091199 .

Vidović, Marija, Battisti, Ilaria, Pantelić, Ana, Morina, Filis, Arrigoni, Giorgio, Masi, Antonio, Veljović-Jovanović, Sonja, "Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study" in Plants, 11, no. 9 (2022):1199,
https://doi.org/10.3390/plants11091199 . .

TY  - CONF
AU  - Milić Komić, Sonja
AU  - Veljović-Jovanović, Sonja
AU  - Pantelic, Ana
AU  - Vidović, Marija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3086
AB  - Ressurection plants are extraordinary because of their ability to withstand long periods without water, enter a state of anhydrobiosis, and fully recover upon water arrival. Ramonda serbica is a relic and endemic species that belong to a very small group of desiccation-tolerant plants in Europe. Underlying physiological, molecular and morphological mechanisms that enable these plants to survive harsh environmental conditions have been an appealing subject to many researchers. Most of the genes responsible for this amazing ability are present in other plants, and this path of research where those genes could be activated in crops is growing much more attention because of the imminent crisis regarding food supplies in the near future. Key components involved in the response to dehydration in R. serbica plants were analysed through a comprehensive transcriptomic, proteomic, metabolite and photosynthetic study. Late embryogenesis abundant proteins play a significant role in the complex defence processes involved in desiccation tolerance. Defining physicochemical characteristics and specific physiological functions of late embryogenesis abundant proteins – LEAPs may lead to their applicability in other areas of research.
PB  - Belgrade : Faculty of Chemistry, Serbian Biochemical Society
C3  - Serbian Biochemical Society Eleventh Conference (scientific meeting of an international character) - "Amazing Biochemistry"
T1  - Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein
SP  - 37
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3086
ER  - 

@conference{
author = "Milić Komić, Sonja and Veljović-Jovanović, Sonja and Pantelic, Ana and Vidović, Marija",
year = "2022",
abstract = "Ressurection plants are extraordinary because of their ability to withstand long periods without water, enter a state of anhydrobiosis, and fully recover upon water arrival. Ramonda serbica is a relic and endemic species that belong to a very small group of desiccation-tolerant plants in Europe. Underlying physiological, molecular and morphological mechanisms that enable these plants to survive harsh environmental conditions have been an appealing subject to many researchers. Most of the genes responsible for this amazing ability are present in other plants, and this path of research where those genes could be activated in crops is growing much more attention because of the imminent crisis regarding food supplies in the near future. Key components involved in the response to dehydration in R. serbica plants were analysed through a comprehensive transcriptomic, proteomic, metabolite and photosynthetic study. Late embryogenesis abundant proteins play a significant role in the complex defence processes involved in desiccation tolerance. Defining physicochemical characteristics and specific physiological functions of late embryogenesis abundant proteins – LEAPs may lead to their applicability in other areas of research.",
publisher = "Belgrade : Faculty of Chemistry, Serbian Biochemical Society",
journal = "Serbian Biochemical Society Eleventh Conference (scientific meeting of an international character) - "Amazing Biochemistry"",
title = "Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein",
pages = "37",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3086"
}

Milić Komić, S., Veljović-Jovanović, S., Pantelic, A.,& Vidović, M.. (2022). Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein. in Serbian Biochemical Society Eleventh Conference (scientific meeting of an international character) - "Amazing Biochemistry"
Belgrade : Faculty of Chemistry, Serbian Biochemical Society., 37.
https://hdl.handle.net/21.15107/rcub_rimsi_3086

Milić Komić S, Veljović-Jovanović S, Pantelic A, Vidović M. Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein. in Serbian Biochemical Society Eleventh Conference (scientific meeting of an international character) - "Amazing Biochemistry". 2022;:37.
https://hdl.handle.net/21.15107/rcub_rimsi_3086 .

Milić Komić, Sonja, Veljović-Jovanović, Sonja, Pantelic, Ana, Vidović, Marija, "Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein" in Serbian Biochemical Society Eleventh Conference (scientific meeting of an international character) - "Amazing Biochemistry" (2022):37,
https://hdl.handle.net/21.15107/rcub_rimsi_3086 .

TY  - CONF
AU  - Pantelic, Ana
AU  - Stevanovic, Strahinja
AU  - Milić, Dejana
AU  - Milić Komić, Sonja
AU  - Kilibarda, Nataša
AU  - Vidović, Marija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3085
AB  - An ancient resurrection plant Ramonda serbica Panc. is able to survive a long desiccation period and reestablish metabolic activity upon watering. A hallmark of desiccation tolerance in the resurrection species is the accumulation of protective late embryogenesis abundant proteins (LEAPs). These intrinsically disordered proteins (IDPs) may stabilize the correct structure of proteins and membranes during cellular dehydration. The aim of our study was to assess LEA genes’ expression levels in hydrated (HL) and desiccated leaves (DL) and to identify, characterise, and estimate the potential role of R. serbica LEAPs in desiccation tolerance. In total, 318 LEAPs from HL and DL were identified and classified into the seven LEA protein family groups ranging from LEA1-LEA5, seed maturation proteins (SMPs), and dehydrins (DEH). Analysis of the physicochemical properties, motif architecture, secondary structure, homology, and phylogenetic relationships demonstrated that R. serbica LEAPs greatly differed among the LEA family groups. The most abundant LEA2 proteins (mostly downregulated upon desiccation) exhibited lower hydrophilicity and propensity to fold into organised globular domains. Oppositely, hydrophilic LEA4 proteins tended to form amphipathic, A-type, α-helices. Most of desiccation-upregulated LEA genes encoded highly disordered DEH1, LEA1, LEA4.2, and LEA4.3 proteins. While dehydrins might chelate metals and bind DNA under water deficit, other ID LEAPs (e.g. LEA1, LEA3, LEA4) might participate in forming intracellular proteinaceous condensates or adopt amphipathic α-helical conformation, enabling them to stabilise desiccation-sensitive proteins and membranes. Taken together, possible functions of LEAPs are discussed with significant implications on drought tolerance improvement of crops grown in arid areas.
PB  - Serbian Plant Physiology Society
PB  - Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade
PB  - Faculty of Biology, University of Belgrade
C3  - Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade, 2022, 83-
T1  - Late embryogenesis abundant (LEA) proteins in Ramonda serbica Panc identification, classification and structural characterization
SP  - 95
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3085
ER  - 

@conference{
author = "Pantelic, Ana and Stevanovic, Strahinja and Milić, Dejana and Milić Komić, Sonja and Kilibarda, Nataša and Vidović, Marija",
year = "2022",
abstract = "An ancient resurrection plant Ramonda serbica Panc. is able to survive a long desiccation period and reestablish metabolic activity upon watering. A hallmark of desiccation tolerance in the resurrection species is the accumulation of protective late embryogenesis abundant proteins (LEAPs). These intrinsically disordered proteins (IDPs) may stabilize the correct structure of proteins and membranes during cellular dehydration. The aim of our study was to assess LEA genes’ expression levels in hydrated (HL) and desiccated leaves (DL) and to identify, characterise, and estimate the potential role of R. serbica LEAPs in desiccation tolerance. In total, 318 LEAPs from HL and DL were identified and classified into the seven LEA protein family groups ranging from LEA1-LEA5, seed maturation proteins (SMPs), and dehydrins (DEH). Analysis of the physicochemical properties, motif architecture, secondary structure, homology, and phylogenetic relationships demonstrated that R. serbica LEAPs greatly differed among the LEA family groups. The most abundant LEA2 proteins (mostly downregulated upon desiccation) exhibited lower hydrophilicity and propensity to fold into organised globular domains. Oppositely, hydrophilic LEA4 proteins tended to form amphipathic, A-type, α-helices. Most of desiccation-upregulated LEA genes encoded highly disordered DEH1, LEA1, LEA4.2, and LEA4.3 proteins. While dehydrins might chelate metals and bind DNA under water deficit, other ID LEAPs (e.g. LEA1, LEA3, LEA4) might participate in forming intracellular proteinaceous condensates or adopt amphipathic α-helical conformation, enabling them to stabilise desiccation-sensitive proteins and membranes. Taken together, possible functions of LEAPs are discussed with significant implications on drought tolerance improvement of crops grown in arid areas.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade, Faculty of Biology, University of Belgrade",
journal = "Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade, 2022, 83-",
title = "Late embryogenesis abundant (LEA) proteins in Ramonda serbica Panc identification, classification and structural characterization",
pages = "95",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3085"
}

Pantelic, A., Stevanovic, S., Milić, D., Milić Komić, S., Kilibarda, N.,& Vidović, M.. (2022). Late embryogenesis abundant (LEA) proteins in Ramonda serbica Panc identification, classification and structural characterization. in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade, 2022, 83-
Serbian Plant Physiology Society., 95.
https://hdl.handle.net/21.15107/rcub_rimsi_3085

Pantelic A, Stevanovic S, Milić D, Milić Komić S, Kilibarda N, Vidović M. Late embryogenesis abundant (LEA) proteins in Ramonda serbica Panc identification, classification and structural characterization. in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade, 2022, 83-. 2022;:95.
https://hdl.handle.net/21.15107/rcub_rimsi_3085 .

Pantelic, Ana, Stevanovic, Strahinja, Milić, Dejana, Milić Komić, Sonja, Kilibarda, Nataša, Vidović, Marija, "Late embryogenesis abundant (LEA) proteins in Ramonda serbica Panc identification, classification and structural characterization" in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade, 2022, 83- (2022):95,
https://hdl.handle.net/21.15107/rcub_rimsi_3085 .

TY  - CONF
AU  - Živanović, Bojana
AU  - Prokić, Ljiljana
AU  - Milić Komić, Sonja
AU  - Nikolić, Nenad
AU  - Sedlarević Zorić, Ana
AU  - Vidović, Marija
AU  - Veljović-Jovanović, Sonja
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1871
AB  - The objective of this study was to determine the constitutive differences in physiological, biochemical and morphological parameters between two tomato genotypes with different levels of abscisic acid (ABA) – wild type Ailsa Craig (WT) and ABA deficient mutant flacca. Due to reduced ABA biosynthesis, flacca mutant is a suitable model system for investigating the influence of endogenous leaf ABA level in response to drought in plants. Within this research, plants were grown under controlled conditions at 800 μmol m-2s-1, until the end of the vegetative phase when samples were taken. The accumulation of the most abundant soluble sugars, sorbitol, phenolic compounds, and ascorbate in the leaves, as well as the cell walls compounds, were analyzed. Lower constitutive ABA content in flacca was accompanied by two times higher stomatal conductance and similar leaf water potential. Higher content of phenolic compounds (HBAs, HCAs, flavonoids) was determined in WT plants, which was in contrast with the elevated accumulation of the epidermal flavonoids in flacca. Larger accumulation of sorbitol in WT, and of the most abundant soluble sugars (glucose, fructose and sucrose) indicate that flacca accumulates lower content of osmolytes which was opposite to the condition at low light. However, an alternative mechanism related to cell wall modulation imposed its importance in the development of plant acclimation mechanisms under stressful environmental conditions in tomato deficient in ABA. On the other hand, an elevated ascorbate redox state in flacca indicates a higher sensitivity to oxidative stress of the mutant compared to WT even in optimal environmental conditions.
PB  - Serbian Plant Physiology Society
PB  - Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade
PB  - Faculty of Biology, University of Belgrade
C3  - Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade
T1  - Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca
SP  - 83
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1871
ER  - 

@conference{
author = "Živanović, Bojana and Prokić, Ljiljana and Milić Komić, Sonja and Nikolić, Nenad and Sedlarević Zorić, Ana and Vidović, Marija and Veljović-Jovanović, Sonja",
year = "2022",
abstract = "The objective of this study was to determine the constitutive differences in physiological, biochemical and morphological parameters between two tomato genotypes with different levels of abscisic acid (ABA) – wild type Ailsa Craig (WT) and ABA deficient mutant flacca. Due to reduced ABA biosynthesis, flacca mutant is a suitable model system for investigating the influence of endogenous leaf ABA level in response to drought in plants. Within this research, plants were grown under controlled conditions at 800 μmol m-2s-1, until the end of the vegetative phase when samples were taken. The accumulation of the most abundant soluble sugars, sorbitol, phenolic compounds, and ascorbate in the leaves, as well as the cell walls compounds, were analyzed. Lower constitutive ABA content in flacca was accompanied by two times higher stomatal conductance and similar leaf water potential. Higher content of phenolic compounds (HBAs, HCAs, flavonoids) was determined in WT plants, which was in contrast with the elevated accumulation of the epidermal flavonoids in flacca. Larger accumulation of sorbitol in WT, and of the most abundant soluble sugars (glucose, fructose and sucrose) indicate that flacca accumulates lower content of osmolytes which was opposite to the condition at low light. However, an alternative mechanism related to cell wall modulation imposed its importance in the development of plant acclimation mechanisms under stressful environmental conditions in tomato deficient in ABA. On the other hand, an elevated ascorbate redox state in flacca indicates a higher sensitivity to oxidative stress of the mutant compared to WT even in optimal environmental conditions.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade, Faculty of Biology, University of Belgrade",
journal = "Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade",
title = "Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca",
pages = "83",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1871"
}

Živanović, B., Prokić, L., Milić Komić, S., Nikolić, N., Sedlarević Zorić, A., Vidović, M.,& Veljović-Jovanović, S.. (2022). Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca. in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade
Serbian Plant Physiology Society., 83.
https://hdl.handle.net/21.15107/rcub_rimsi_1871

Živanović B, Prokić L, Milić Komić S, Nikolić N, Sedlarević Zorić A, Vidović M, Veljović-Jovanović S. Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca. in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade. 2022;:83.
https://hdl.handle.net/21.15107/rcub_rimsi_1871 .

Živanović, Bojana, Prokić, Ljiljana, Milić Komić, Sonja, Nikolić, Nenad, Sedlarević Zorić, Ana, Vidović, Marija, Veljović-Jovanović, Sonja, "Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca" in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade (2022):83,
https://hdl.handle.net/21.15107/rcub_rimsi_1871 .

TY  - CONF
AU  - Golob, Aleksandra
AU  - Ojdanič, Nik
AU  - Živanović, Bojana
AU  - Germ, Mateja
AU  - Milić Komić, Sonja
AU  - Sedlarević Zorić, Ana
AU  - Milić, Dejana
AU  - Pantelić, Ana
AU  - Mavrič Čermelj, Anja
AU  - Samardžić, Jelena
AU  - Veljović-Jovanović, Sonja
AU  - Vidović, Marija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1872
AB  - Drought causes huge agricultural and economic losses worldwide. Silicon (Si) is considered a
beneficial element for plants. It mitigates stress caused by salinity, drought, and high and low temperatures
by promoting antioxidant production. However, the underlying mechanisms are still not
elucidated. We investigated Si fertilisation effect on photosynthetic parameters, trichome number,
leaf optical properties, as well as profiles of amino acids and polyphenols in barley exposed to
water shortage. Silicon was applied in three growth stages: (i) before the flag leaf emergence;
(ii) prior to the grain filling phase; (iii) at the grain filling phase start. Drought negatively impacts
photochemical efficiency, stomatal conductance, photosynthetic pigment content, and leaf reflective
and transmissive properties. Si application between flag leaf emergence and grain filling
had the strongest effect on light reflectance. Among all analyzed phenolics, saponarin was the
most abundant in all samples, irrespective of water regime and Si supply. Caffeoyl ester was the
only hydroxycinnamic acid showing significant accumulation with the latest applied Si compared
to no added Si upon drought. The major amino acids in barley leaves were glutamate, glutamine,
aspartate, asparagine, and serine. Aspartate content was the highest in leaves exposed to drought
without Si addition, while lysine was the most accumulated in the leaves supplemented by Si at
the grain filling phase start. Proline was 2.5 times more abundant in the leaves exposed to drought
regardless of Si treatment. Taken together, although Si did not mitigate drought stress effects, its
effect was dependent on the barley growth phase prior to supplementation.
PB  - Serbian Plant Physiology Society
PB  - Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade
PB  - Faculty of Biology, University of Belgrade
C3  - Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade
T1  - The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought
SP  - 67
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1872
ER  - 

@conference{
author = "Golob, Aleksandra and Ojdanič, Nik and Živanović, Bojana and Germ, Mateja and Milić Komić, Sonja and Sedlarević Zorić, Ana and Milić, Dejana and Pantelić, Ana and Mavrič Čermelj, Anja and Samardžić, Jelena and Veljović-Jovanović, Sonja and Vidović, Marija",
year = "2022",
abstract = "Drought causes huge agricultural and economic losses worldwide. Silicon (Si) is considered a
beneficial element for plants. It mitigates stress caused by salinity, drought, and high and low temperatures
by promoting antioxidant production. However, the underlying mechanisms are still not
elucidated. We investigated Si fertilisation effect on photosynthetic parameters, trichome number,
leaf optical properties, as well as profiles of amino acids and polyphenols in barley exposed to
water shortage. Silicon was applied in three growth stages: (i) before the flag leaf emergence;
(ii) prior to the grain filling phase; (iii) at the grain filling phase start. Drought negatively impacts
photochemical efficiency, stomatal conductance, photosynthetic pigment content, and leaf reflective
and transmissive properties. Si application between flag leaf emergence and grain filling
had the strongest effect on light reflectance. Among all analyzed phenolics, saponarin was the
most abundant in all samples, irrespective of water regime and Si supply. Caffeoyl ester was the
only hydroxycinnamic acid showing significant accumulation with the latest applied Si compared
to no added Si upon drought. The major amino acids in barley leaves were glutamate, glutamine,
aspartate, asparagine, and serine. Aspartate content was the highest in leaves exposed to drought
without Si addition, while lysine was the most accumulated in the leaves supplemented by Si at
the grain filling phase start. Proline was 2.5 times more abundant in the leaves exposed to drought
regardless of Si treatment. Taken together, although Si did not mitigate drought stress effects, its
effect was dependent on the barley growth phase prior to supplementation.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade, Faculty of Biology, University of Belgrade",
journal = "Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade",
title = "The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought",
pages = "67",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1872"
}

Golob, A., Ojdanič, N., Živanović, B., Germ, M., Milić Komić, S., Sedlarević Zorić, A., Milić, D., Pantelić, A., Mavrič Čermelj, A., Samardžić, J., Veljović-Jovanović, S.,& Vidović, M.. (2022). The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought. in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade
Serbian Plant Physiology Society., 67.
https://hdl.handle.net/21.15107/rcub_rimsi_1872

Golob A, Ojdanič N, Živanović B, Germ M, Milić Komić S, Sedlarević Zorić A, Milić D, Pantelić A, Mavrič Čermelj A, Samardžić J, Veljović-Jovanović S, Vidović M. The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought. in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade. 2022;:67.
https://hdl.handle.net/21.15107/rcub_rimsi_1872 .

Golob, Aleksandra, Ojdanič, Nik, Živanović, Bojana, Germ, Mateja, Milić Komić, Sonja, Sedlarević Zorić, Ana, Milić, Dejana, Pantelić, Ana, Mavrič Čermelj, Anja, Samardžić, Jelena, Veljović-Jovanović, Sonja, Vidović, Marija, "The usage of silicon fertilisation in order to mitigate the oxidative stress and to improve the resilience of barley subjected to drought" in Book of Abstracts / 4th International Conference on Plant Biology [and] 23rd SPPS Meeting, 6-8 October 2022, Belgrade (2022):67,
https://hdl.handle.net/21.15107/rcub_rimsi_1872 .

TY  - CONF
AU  - Milić Komić, Sonja
AU  - Živanović, Bojana
AU  - Sedlarević Zorić, Ana
AU  - Vidović, Marija
AU  - Veljović-Jovanović, Sonja
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1873
AB  - Three varieties of basil (Ocimum x citriodorum, Ocimum basilicum var. Genovese and Ocimum
basilicum var. purpurascens) were used to examine the effect of different PAR intensities (100, 400
and 1400 µmol photons m-2s-1), as well as different UV-B/PAR ratios, on leaf phenolics accumulation, and components of the ascorbate-glutathione cycle. Phenolic compounds represent the
most abundant class of secondary metabolites, and their function in plants involves protection
against numerous abiotic stresses, role in growth and development, flowering, reproduction and
seed dispersion. A preferential accumulation of HCAs over Flav, induction of class III POD activity
and decreased ascorbate content were characteristic responses to pro-oxidative effect of the high
UV-B/PAR ratio in all three basil varieties. The most remarkable result was the lack of accumulation
of epidermal UV absorbing substances, which appeared to be a crucial photoprotective mechanism in sunlight. The contrasting effect of ecologically relevant UV-B radiation on basil plants,
pro-oxidative vs. acclimative, was determined by the quality and intensity of the background light.
Acclimation of basil to high light comprises a number of processes, among which are the accumulation of epidermal flavonoids and total leaf phenolics, antioxidant response (increase in Asc and
GSH) and lack of downregulation of PSII upon increasing light intensity. Among varieties that we
used as model system in this study, purple basil, with the highest constitutive amount of anthocyanidins, showed the least induction of epidermal flavonoids and lack of light effect upon transfer
from initial 50 to 250 µmol photons m-2s-1.
PB  - Serbian Plant Physiology Society  Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia,  University of Belgrade  Faculty of Biology, University of Belgrade
C3  - 4th International Conference on Plant Biology (23rd SPPS Meeting)
T1  - Distinctive regulation of different phenolics biosynthesis by high light and UV-B in three basil varieties
SP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1873
ER  - 

@conference{
author = "Milić Komić, Sonja and Živanović, Bojana and Sedlarević Zorić, Ana and Vidović, Marija and Veljović-Jovanović, Sonja",
year = "2022",
abstract = "Three varieties of basil (Ocimum x citriodorum, Ocimum basilicum var. Genovese and Ocimum
basilicum var. purpurascens) were used to examine the effect of different PAR intensities (100, 400
and 1400 µmol photons m-2s-1), as well as different UV-B/PAR ratios, on leaf phenolics accumulation, and components of the ascorbate-glutathione cycle. Phenolic compounds represent the
most abundant class of secondary metabolites, and their function in plants involves protection
against numerous abiotic stresses, role in growth and development, flowering, reproduction and
seed dispersion. A preferential accumulation of HCAs over Flav, induction of class III POD activity
and decreased ascorbate content were characteristic responses to pro-oxidative effect of the high
UV-B/PAR ratio in all three basil varieties. The most remarkable result was the lack of accumulation
of epidermal UV absorbing substances, which appeared to be a crucial photoprotective mechanism in sunlight. The contrasting effect of ecologically relevant UV-B radiation on basil plants,
pro-oxidative vs. acclimative, was determined by the quality and intensity of the background light.
Acclimation of basil to high light comprises a number of processes, among which are the accumulation of epidermal flavonoids and total leaf phenolics, antioxidant response (increase in Asc and
GSH) and lack of downregulation of PSII upon increasing light intensity. Among varieties that we
used as model system in this study, purple basil, with the highest constitutive amount of anthocyanidins, showed the least induction of epidermal flavonoids and lack of light effect upon transfer
from initial 50 to 250 µmol photons m-2s-1.",
publisher = "Serbian Plant Physiology Society  Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia,  University of Belgrade  Faculty of Biology, University of Belgrade",
journal = "4th International Conference on Plant Biology (23rd SPPS Meeting)",
title = "Distinctive regulation of different phenolics biosynthesis by high light and UV-B in three basil varieties",
pages = "43",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1873"
}

Milić Komić, S., Živanović, B., Sedlarević Zorić, A., Vidović, M.,& Veljović-Jovanović, S.. (2022). Distinctive regulation of different phenolics biosynthesis by high light and UV-B in three basil varieties. in 4th International Conference on Plant Biology (23rd SPPS Meeting)
Serbian Plant Physiology Society  Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia,  University of Belgrade  Faculty of Biology, University of Belgrade., 43.
https://hdl.handle.net/21.15107/rcub_rimsi_1873

Milić Komić S, Živanović B, Sedlarević Zorić A, Vidović M, Veljović-Jovanović S. Distinctive regulation of different phenolics biosynthesis by high light and UV-B in three basil varieties. in 4th International Conference on Plant Biology (23rd SPPS Meeting). 2022;:43.
https://hdl.handle.net/21.15107/rcub_rimsi_1873 .

Milić Komić, Sonja, Živanović, Bojana, Sedlarević Zorić, Ana, Vidović, Marija, Veljović-Jovanović, Sonja, "Distinctive regulation of different phenolics biosynthesis by high light and UV-B in three basil varieties" in 4th International Conference on Plant Biology (23rd SPPS Meeting) (2022):43,
https://hdl.handle.net/21.15107/rcub_rimsi_1873 .

TY  - JOUR
AU  - Pantelić, Ana
AU  - Stevanović, Strahinja
AU  - Milić Komić, Sonja
AU  - Kilibarda, Nataša
AU  - Vidović, Marija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1575
AB  - Ramonda serbica Panc. is an ancient resurrection plant able to survive a long desiccation period and recover metabolic functions upon watering. The accumulation of protective late embryogenesis abundant proteins (LEAPs) is a desiccation tolerance hallmark. To propose their role in R. serbica desiccation tolerance, we structurally characterised LEAPs and evaluated LEA gene expression levels in hydrated and desiccated leaves. By integrating de novo transcriptomics and homologues LEAP domains, 318 R. serbica LEAPs were identified and classified according to their conserved motifs and phylogeny. The in silico analysis revealed that hydrophilic LEA4 proteins exhibited an exceptionally high tendency to form amphipathic α‐helices. The most abundant, atypical LEA2 group contained more hydrophobic proteins predicted to fold into the defined globular domains. Within the desiccation‐upregulated LEA genes, the majority encoded highly disordered DEH1, LEA1, LEA4.2, and LEA4.3 proteins, while the greatest portion of downregulated genes encoded LEA2.3 and LEA2.5 proteins. While dehydrins might chelate metals and bind DNA under water deficit, other intrinsically disordered LEAPs might participate in forming intracellular proteinaceous condensates or adopt amphipathic α‐helical conformation, enabling them to stabilise desiccation‐sensitive proteins and membranes. This comprehensive LEAPs structural characterisation is essential to understanding their function and regulation during desiccation aiming at crop drought tolerance improvement.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - In Silico Characterisation of the Late Embryogenesis Abundant (LEA) Protein Families and Their Role in Desiccation Tolerance in Ramonda serbica Panc.
IS  - 7
SP  - 3547
VL  - 23
DO  - 10.3390/ijms23073547
ER  - 

@article{
author = "Pantelić, Ana and Stevanović, Strahinja and Milić Komić, Sonja and Kilibarda, Nataša and Vidović, Marija",
year = "2022",
abstract = "Ramonda serbica Panc. is an ancient resurrection plant able to survive a long desiccation period and recover metabolic functions upon watering. The accumulation of protective late embryogenesis abundant proteins (LEAPs) is a desiccation tolerance hallmark. To propose their role in R. serbica desiccation tolerance, we structurally characterised LEAPs and evaluated LEA gene expression levels in hydrated and desiccated leaves. By integrating de novo transcriptomics and homologues LEAP domains, 318 R. serbica LEAPs were identified and classified according to their conserved motifs and phylogeny. The in silico analysis revealed that hydrophilic LEA4 proteins exhibited an exceptionally high tendency to form amphipathic α‐helices. The most abundant, atypical LEA2 group contained more hydrophobic proteins predicted to fold into the defined globular domains. Within the desiccation‐upregulated LEA genes, the majority encoded highly disordered DEH1, LEA1, LEA4.2, and LEA4.3 proteins, while the greatest portion of downregulated genes encoded LEA2.3 and LEA2.5 proteins. While dehydrins might chelate metals and bind DNA under water deficit, other intrinsically disordered LEAPs might participate in forming intracellular proteinaceous condensates or adopt amphipathic α‐helical conformation, enabling them to stabilise desiccation‐sensitive proteins and membranes. This comprehensive LEAPs structural characterisation is essential to understanding their function and regulation during desiccation aiming at crop drought tolerance improvement.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "In Silico Characterisation of the Late Embryogenesis Abundant (LEA) Protein Families and Their Role in Desiccation Tolerance in Ramonda serbica Panc.",
number = "7",
pages = "3547",
volume = "23",
doi = "10.3390/ijms23073547"
}

Pantelić, A., Stevanović, S., Milić Komić, S., Kilibarda, N.,& Vidović, M.. (2022). In Silico Characterisation of the Late Embryogenesis Abundant (LEA) Protein Families and Their Role in Desiccation Tolerance in Ramonda serbica Panc.. in International Journal of Molecular Sciences
MDPI., 23(7), 3547.
https://doi.org/10.3390/ijms23073547

Pantelić A, Stevanović S, Milić Komić S, Kilibarda N, Vidović M. In Silico Characterisation of the Late Embryogenesis Abundant (LEA) Protein Families and Their Role in Desiccation Tolerance in Ramonda serbica Panc.. in International Journal of Molecular Sciences. 2022;23(7):3547.
doi:10.3390/ijms23073547 .

Pantelić, Ana, Stevanović, Strahinja, Milić Komić, Sonja, Kilibarda, Nataša, Vidović, Marija, "In Silico Characterisation of the Late Embryogenesis Abundant (LEA) Protein Families and Their Role in Desiccation Tolerance in Ramonda serbica Panc." in International Journal of Molecular Sciences, 23, no. 7 (2022):3547,
https://doi.org/10.3390/ijms23073547 . .

TY  - CONF
AU  - Nikolic, Ljiljana
AU  - Vidović, Marija
AU  - Todorovic, Natasa
AU  - Petkovic, Branka
AU  - Stojadinovic, Gordana
AU  - Martac, Ljiljana
AU  - Bogdanović Pristov, Jelena
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2008
AB  - Investigating membrane properties of plants is a challenging task, considering that success of 
experiments is highly dependent on the possibility to isolate metabolically active protoplasts that 
can withstand membrane current recordings. The aim of the present work is to obtain viable 
protoplasts derived from root cells of Pisum sativum that can be used for the whole-cell patch
clamp. We designed the procedure of the pea protoplasts isolation that delivers stable protoplasts 
with preserved membrane integrity suitable for electrophysiological experiments. We applied a 
custom approach for patch-clamping protoplasts using a microscope with a movable microscope 
stage. We recorded prominent inward and prominent outward types of membrane current profiles of protoplasts. Obtained data indicate that optimized isolation protocol and custom system for patch clamping, can be applied to study membrane properties of root protoplasts.
PB  - Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings)
T1  - PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE  WITH A MOVABLE STAGE
EP  - 274
SP  - 271
VL  - 1
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2008
ER  - 

@conference{
author = "Nikolic, Ljiljana and Vidović, Marija and Todorovic, Natasa and Petkovic, Branka and Stojadinovic, Gordana and Martac, Ljiljana and Bogdanović Pristov, Jelena",
year = "2022",
abstract = "Investigating membrane properties of plants is a challenging task, considering that success of 
experiments is highly dependent on the possibility to isolate metabolically active protoplasts that 
can withstand membrane current recordings. The aim of the present work is to obtain viable 
protoplasts derived from root cells of Pisum sativum that can be used for the whole-cell patch
clamp. We designed the procedure of the pea protoplasts isolation that delivers stable protoplasts 
with preserved membrane integrity suitable for electrophysiological experiments. We applied a 
custom approach for patch-clamping protoplasts using a microscope with a movable microscope 
stage. We recorded prominent inward and prominent outward types of membrane current profiles of protoplasts. Obtained data indicate that optimized isolation protocol and custom system for patch clamping, can be applied to study membrane properties of root protoplasts.",
publisher = "Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings)",
title = "PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE  WITH A MOVABLE STAGE",
pages = "274-271",
volume = "1",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2008"
}

Nikolic, L., Vidović, M., Todorovic, N., Petkovic, B., Stojadinovic, G., Martac, L.,& Bogdanović Pristov, J.. (2022). PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE  WITH A MOVABLE STAGE. in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings)
Society of Physical Chemists of Serbia., 1, 271-274.
https://hdl.handle.net/21.15107/rcub_rimsi_2008

Nikolic L, Vidović M, Todorovic N, Petkovic B, Stojadinovic G, Martac L, Bogdanović Pristov J. PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE  WITH A MOVABLE STAGE. in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings). 2022;1:271-274.
https://hdl.handle.net/21.15107/rcub_rimsi_2008 .

Nikolic, Ljiljana, Vidović, Marija, Todorovic, Natasa, Petkovic, Branka, Stojadinovic, Gordana, Martac, Ljiljana, Bogdanović Pristov, Jelena, "PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE  WITH A MOVABLE STAGE" in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings), 1 (2022):271-274,
https://hdl.handle.net/21.15107/rcub_rimsi_2008 .

TY  - CONF
AU  - Milić Komić, Sonja
AU  - Stevanović, Strahinja
AU  - Vidović, Marija
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1491
AB  - Ramonda serbica Panc. is a resurrection plant that can survive long desiccation
periods (extreme loss of cellular water). The accumulation of late embryogenesis abundant
proteins (LEAPs) is a crucial step in desiccation tolerance mechanism. Based on in vitro studies,
LEAPs can be involved in antioxidative defense, ion sequestration, structural stabilization
of both membranes and enzymes during freezing or drying, while by forming intracellular
proteinaceous condensates they increase structural integrity and intracellular viscosity of cells
during desiccation. Here we investigated the antioxidative potential of LEAPs identified by de
novo transcriptomics of R. serbica, based on their primary and secondary confirmation. In our
previous work [1], we displayed the antioxidative capacity of 20 free proteogenic amino acids
(FAA) through determining their hydroxyl radical (•
OH, generated in Fenton reaction) scavenging
rate by using electron paramagnetic resonance. These results served as a basis for generating
a model for prediction of •
OH scavenging activity for selected proteins. In addition, the model
was built based on protein primary sequences, hydrophobicity, 3D structure and predicted
solvent accessible area. Manually curated data for peptides and proteins with experimentally
determined •
OH scavenging rate were used for training and testing. The model was fed into
machine learning algorithm and •
OH scavenging potential scale was created using IC50 values.
By applying our model, we classified 164 LEAPs according to their potential for •
OH scavenging.
Further work will focus on the experimental evaluation of the obtained model by measuring of
the rate of •
OH scavenging in the presence of recombinantly produced LEAPs.
PB  - Elsevier Science Inc, New York
C3  - Free Radical Biology and Medicine
T1  - Hydroxyl radical scavenging potential of the late embryogenesis abundant proteins (LEA) proteins from Ramonda serbica - in silico approach
VL  - 177
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1491
ER  - 

@conference{
author = "Milić Komić, Sonja and Stevanović, Strahinja and Vidović, Marija",
year = "2021",
abstract = "Ramonda serbica Panc. is a resurrection plant that can survive long desiccation
periods (extreme loss of cellular water). The accumulation of late embryogenesis abundant
proteins (LEAPs) is a crucial step in desiccation tolerance mechanism. Based on in vitro studies,
LEAPs can be involved in antioxidative defense, ion sequestration, structural stabilization
of both membranes and enzymes during freezing or drying, while by forming intracellular
proteinaceous condensates they increase structural integrity and intracellular viscosity of cells
during desiccation. Here we investigated the antioxidative potential of LEAPs identified by de
novo transcriptomics of R. serbica, based on their primary and secondary confirmation. In our
previous work [1], we displayed the antioxidative capacity of 20 free proteogenic amino acids
(FAA) through determining their hydroxyl radical (•
OH, generated in Fenton reaction) scavenging
rate by using electron paramagnetic resonance. These results served as a basis for generating
a model for prediction of •
OH scavenging activity for selected proteins. In addition, the model
was built based on protein primary sequences, hydrophobicity, 3D structure and predicted
solvent accessible area. Manually curated data for peptides and proteins with experimentally
determined •
OH scavenging rate were used for training and testing. The model was fed into
machine learning algorithm and •
OH scavenging potential scale was created using IC50 values.
By applying our model, we classified 164 LEAPs according to their potential for •
OH scavenging.
Further work will focus on the experimental evaluation of the obtained model by measuring of
the rate of •
OH scavenging in the presence of recombinantly produced LEAPs.",
publisher = "Elsevier Science Inc, New York",
journal = "Free Radical Biology and Medicine",
title = "Hydroxyl radical scavenging potential of the late embryogenesis abundant proteins (LEA) proteins from Ramonda serbica - in silico approach",
volume = "177",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1491"
}

Milić Komić, S., Stevanović, S.,& Vidović, M.. (2021). Hydroxyl radical scavenging potential of the late embryogenesis abundant proteins (LEA) proteins from Ramonda serbica - in silico approach. in Free Radical Biology and Medicine
Elsevier Science Inc, New York., 177.
https://hdl.handle.net/21.15107/rcub_rimsi_1491

Milić Komić S, Stevanović S, Vidović M. Hydroxyl radical scavenging potential of the late embryogenesis abundant proteins (LEA) proteins from Ramonda serbica - in silico approach. in Free Radical Biology and Medicine. 2021;177.
https://hdl.handle.net/21.15107/rcub_rimsi_1491 .

Milić Komić, Sonja, Stevanović, Strahinja, Vidović, Marija, "Hydroxyl radical scavenging potential of the late embryogenesis abundant proteins (LEA) proteins from Ramonda serbica - in silico approach" in Free Radical Biology and Medicine, 177 (2021),
https://hdl.handle.net/21.15107/rcub_rimsi_1491 .

TY  - CONF
AU  - Markovic, Nemanja
AU  - Milić Komić, Sonja
AU  - Radosavljevic, Jelena
AU  - Pantelic, Ana
AU  - Kilibarda, Nataša
AU  - Vidović, Marija
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3084
AB  - Late embryogenesis abundant (LEA) proteins are induced in cellular dehydration, such as freezing, drought, or desiccation. They can be involved in antioxidative defense, ion sequestration, and structural stabilization of both membranes and enzymes during freezing or drying, while by forming intracellular proteinaceous condensates they increase structural integrity and intracellular viscosity of cells during desiccation 1. The genome of the model plant Arabidopsis thaliana contains 51 genes encoding LEA proteins2. The majority of these LEA proteins (35%) belongs to Pfam LEA_4 (PF02987) family. In silico analysis suggested that these proteins are highly hydrophilic proteins with significant intrinsically disordered protein (IDP) properties. In order to evaluate structural properties and possible functions of LEA_4 protein family under different water content, a representative AtLEA25 protein (At2g42560, 635 aa), naturally located in the cytoplasm of seeds3 was obtained in Escherichia coli by recombinant DNA technology. Although this technology has been traditionally used to over-express and purify various globular proteins, numerous reports have shown that the IDPs, due to their structural plasicity are naturally highly susceptible to proteolytic cleavage. To conduct structural and functional studies we developed a robust method to produce highly purified (>95% pure) AtLEA25 with no detectable amount of protein breakdown products.
PB  - Belgrade : Serbian Biochemical Society
C3  - Biochemical Insights into Molecular Mechanisms, 2021, 117-
T1  - Efficient production of highly purified Late Embryogenesis Abundant (LEA) protein from Arabidopsis thaliana by recombinant DNA technology
EP  - 99
SP  - 98
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3084
ER  - 

@conference{
author = "Markovic, Nemanja and Milić Komić, Sonja and Radosavljevic, Jelena and Pantelic, Ana and Kilibarda, Nataša and Vidović, Marija",
year = "2021",
abstract = "Late embryogenesis abundant (LEA) proteins are induced in cellular dehydration, such as freezing, drought, or desiccation. They can be involved in antioxidative defense, ion sequestration, and structural stabilization of both membranes and enzymes during freezing or drying, while by forming intracellular proteinaceous condensates they increase structural integrity and intracellular viscosity of cells during desiccation 1. The genome of the model plant Arabidopsis thaliana contains 51 genes encoding LEA proteins2. The majority of these LEA proteins (35%) belongs to Pfam LEA_4 (PF02987) family. In silico analysis suggested that these proteins are highly hydrophilic proteins with significant intrinsically disordered protein (IDP) properties. In order to evaluate structural properties and possible functions of LEA_4 protein family under different water content, a representative AtLEA25 protein (At2g42560, 635 aa), naturally located in the cytoplasm of seeds3 was obtained in Escherichia coli by recombinant DNA technology. Although this technology has been traditionally used to over-express and purify various globular proteins, numerous reports have shown that the IDPs, due to their structural plasicity are naturally highly susceptible to proteolytic cleavage. To conduct structural and functional studies we developed a robust method to produce highly purified (>95% pure) AtLEA25 with no detectable amount of protein breakdown products.",
publisher = "Belgrade : Serbian Biochemical Society",
journal = "Biochemical Insights into Molecular Mechanisms, 2021, 117-",
title = "Efficient production of highly purified Late Embryogenesis Abundant (LEA) protein from Arabidopsis thaliana by recombinant DNA technology",
pages = "99-98",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3084"
}

Markovic, N., Milić Komić, S., Radosavljevic, J., Pantelic, A., Kilibarda, N.,& Vidović, M.. (2021). Efficient production of highly purified Late Embryogenesis Abundant (LEA) protein from Arabidopsis thaliana by recombinant DNA technology. in Biochemical Insights into Molecular Mechanisms, 2021, 117-
Belgrade : Serbian Biochemical Society., 98-99.
https://hdl.handle.net/21.15107/rcub_rimsi_3084

Markovic N, Milić Komić S, Radosavljevic J, Pantelic A, Kilibarda N, Vidović M. Efficient production of highly purified Late Embryogenesis Abundant (LEA) protein from Arabidopsis thaliana by recombinant DNA technology. in Biochemical Insights into Molecular Mechanisms, 2021, 117-. 2021;:98-99.
https://hdl.handle.net/21.15107/rcub_rimsi_3084 .

Markovic, Nemanja, Milić Komić, Sonja, Radosavljevic, Jelena, Pantelic, Ana, Kilibarda, Nataša, Vidović, Marija, "Efficient production of highly purified Late Embryogenesis Abundant (LEA) protein from Arabidopsis thaliana by recombinant DNA technology" in Biochemical Insights into Molecular Mechanisms, 2021, 117- (2021):98-99,
https://hdl.handle.net/21.15107/rcub_rimsi_3084 .

TY  - CONF
AU  - Pantelic, Ana
AU  - Stevanovic, Strahinja
AU  - Kilibarda, Nataša
AU  - Milić Komić, Sonja
AU  - Radosavljevic, Jelena
AU  - Vidović, Marija
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3083
AB  - Endemic plant species, Ramonda serbica is a resurrection plant that can tolerate extreme dehydration (desiccation, loss of 95% of cellular water) even over months. The accumulation of late embryogenesis abundant proteins (LEAPs) is a crucial step in the mechanism of desiccation tolerance. The role of LEAPs is not completely resolved, but they are accepted as intrinsically disordered proteins (IDPs). Based on previously established de novo transcriptome database of R. serbica leaves we identify around 160 members of LEA gene family. Identified LEAPs were classified into six groups: LEA 1-5 and seed maturation proteins (SMPs) according to protein family (Pfam) database. Based on multiple sequence alignment, secondary structure prediction and 3D structure modeling, we conducted LEA protein structure analysis. We showed that more than 50% of identified LEAPs exhibited a high propensity to form α-helices. As predicted by several bioinformatic tools, more than 70% of identified LEAPs were found to be highly disordered. Thus, these proteins are predicted to be disordered in solution, but they acquire a secondary, predominantly α-helical structure during drying, in contrast to globular proteins, which most often causes the loss of structure upon dehydration. By using molecular dynamic simulations, we identified the most favorable conformations of representative LEAPs and we have studied conformational transitions driven by the water scarcity. Structural characterization of LEAPs is a key to understand their function and regulation of their intrinsic structural disorder-to-order transition during desiccation as a requirement for biological function, in order to promote development of new therapeutic strategies in neurodegenerative disorders, cell preservation technology and the improvement of crop drought tolerance.
PB  - Belgrade : Serbian Biochemical Society
C3  - Biochemical Insights into Molecular Mechanisms, 2021, 117-
T1  - Characterization of the late embryogenesis abundant (LEA) proteins family in hydrated and desiccated Ramonda serbica Panc. leaves
EP  - 118
SP  - 117
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3083
ER  - 

@conference{
author = "Pantelic, Ana and Stevanovic, Strahinja and Kilibarda, Nataša and Milić Komić, Sonja and Radosavljevic, Jelena and Vidović, Marija",
year = "2021",
abstract = "Endemic plant species, Ramonda serbica is a resurrection plant that can tolerate extreme dehydration (desiccation, loss of 95% of cellular water) even over months. The accumulation of late embryogenesis abundant proteins (LEAPs) is a crucial step in the mechanism of desiccation tolerance. The role of LEAPs is not completely resolved, but they are accepted as intrinsically disordered proteins (IDPs). Based on previously established de novo transcriptome database of R. serbica leaves we identify around 160 members of LEA gene family. Identified LEAPs were classified into six groups: LEA 1-5 and seed maturation proteins (SMPs) according to protein family (Pfam) database. Based on multiple sequence alignment, secondary structure prediction and 3D structure modeling, we conducted LEA protein structure analysis. We showed that more than 50% of identified LEAPs exhibited a high propensity to form α-helices. As predicted by several bioinformatic tools, more than 70% of identified LEAPs were found to be highly disordered. Thus, these proteins are predicted to be disordered in solution, but they acquire a secondary, predominantly α-helical structure during drying, in contrast to globular proteins, which most often causes the loss of structure upon dehydration. By using molecular dynamic simulations, we identified the most favorable conformations of representative LEAPs and we have studied conformational transitions driven by the water scarcity. Structural characterization of LEAPs is a key to understand their function and regulation of their intrinsic structural disorder-to-order transition during desiccation as a requirement for biological function, in order to promote development of new therapeutic strategies in neurodegenerative disorders, cell preservation technology and the improvement of crop drought tolerance.",
publisher = "Belgrade : Serbian Biochemical Society",
journal = "Biochemical Insights into Molecular Mechanisms, 2021, 117-",
title = "Characterization of the late embryogenesis abundant (LEA) proteins family in hydrated and desiccated Ramonda serbica Panc. leaves",
pages = "118-117",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3083"
}

Pantelic, A., Stevanovic, S., Kilibarda, N., Milić Komić, S., Radosavljevic, J.,& Vidović, M.. (2021). Characterization of the late embryogenesis abundant (LEA) proteins family in hydrated and desiccated Ramonda serbica Panc. leaves. in Biochemical Insights into Molecular Mechanisms, 2021, 117-
Belgrade : Serbian Biochemical Society., 117-118.
https://hdl.handle.net/21.15107/rcub_rimsi_3083

Pantelic A, Stevanovic S, Kilibarda N, Milić Komić S, Radosavljevic J, Vidović M. Characterization of the late embryogenesis abundant (LEA) proteins family in hydrated and desiccated Ramonda serbica Panc. leaves. in Biochemical Insights into Molecular Mechanisms, 2021, 117-. 2021;:117-118.
https://hdl.handle.net/21.15107/rcub_rimsi_3083 .

Pantelic, Ana, Stevanovic, Strahinja, Kilibarda, Nataša, Milić Komić, Sonja, Radosavljevic, Jelena, Vidović, Marija, "Characterization of the late embryogenesis abundant (LEA) proteins family in hydrated and desiccated Ramonda serbica Panc. leaves" in Biochemical Insights into Molecular Mechanisms, 2021, 117- (2021):117-118,
https://hdl.handle.net/21.15107/rcub_rimsi_3083 .

TY  - CHAP
AU  - Vidović, Marija
AU  - Milić Komić, Sonja
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2928
AB  - Proteolysis represents a primary recycling system for amino acids. Moreover, by controlling the protein turnover, proteolysis plays an important role in key cellular processes such as control of cell cycle, programmed cell death (including senescence) and response to various stimuli. In eukaryotic cells, most proteins are degraded by autophagy and 26S proteasome machinery, composed in general of 20S proteolytic core and 19S regulatory particle. The protein susceptibility to proteolysis is determined by its structural features. Folded globular proteins in their native state are rarely degraded, however when misfolded, denatured or when an unstructured region is attached, the degradation is promoted. Proteins natively containing intrinsically disordered regions (IDRs) or completely lacking the stable secondary and tertiary structures are defined as intrinsically disordered proteins (IDPs). Due to their high intramolecular flexibility and plasticity, IDPs are involved in DNA metabolism, transcriptional activation, autophagy, and signalling cascades related to response to various stimuli. In sessile organisms such as plants, IDPs enable prompt acclimation to external factors, including light perception, adaptation to oxidative stress and water loss, and regulation of protective, antioxidative and secondary metabolism. Binding of specific ligands and partners to particular IDP triggers structural changes and affects the stability of IDP, its susceptibility to proteolysis and aggregation-propensity. Theabnormal aggregation of several IDPs and altered proteolysis pathways are closely connected with serious neurodegenerative disorders, such as Alzheimer’s and Parkinson's diseases. In this chapter, we discuss the current understanding of proteolytic processes of specific, well-characterised IDPs under different physiological states, emphasizing the influence of the microenvironment and ligands/partners on their conformation.
T2  - A closer look at proteolysis
T1  - Regulation of proteolysis of intrinsically disordered proteins: physiological consequences
EP  - 156
SP  - 111
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2928
ER  - 

@inbook{
author = "Vidović, Marija and Milić Komić, Sonja",
year = "2021",
abstract = "Proteolysis represents a primary recycling system for amino acids. Moreover, by controlling the protein turnover, proteolysis plays an important role in key cellular processes such as control of cell cycle, programmed cell death (including senescence) and response to various stimuli. In eukaryotic cells, most proteins are degraded by autophagy and 26S proteasome machinery, composed in general of 20S proteolytic core and 19S regulatory particle. The protein susceptibility to proteolysis is determined by its structural features. Folded globular proteins in their native state are rarely degraded, however when misfolded, denatured or when an unstructured region is attached, the degradation is promoted. Proteins natively containing intrinsically disordered regions (IDRs) or completely lacking the stable secondary and tertiary structures are defined as intrinsically disordered proteins (IDPs). Due to their high intramolecular flexibility and plasticity, IDPs are involved in DNA metabolism, transcriptional activation, autophagy, and signalling cascades related to response to various stimuli. In sessile organisms such as plants, IDPs enable prompt acclimation to external factors, including light perception, adaptation to oxidative stress and water loss, and regulation of protective, antioxidative and secondary metabolism. Binding of specific ligands and partners to particular IDP triggers structural changes and affects the stability of IDP, its susceptibility to proteolysis and aggregation-propensity. Theabnormal aggregation of several IDPs and altered proteolysis pathways are closely connected with serious neurodegenerative disorders, such as Alzheimer’s and Parkinson's diseases. In this chapter, we discuss the current understanding of proteolytic processes of specific, well-characterised IDPs under different physiological states, emphasizing the influence of the microenvironment and ligands/partners on their conformation.",
journal = "A closer look at proteolysis",
booktitle = "Regulation of proteolysis of intrinsically disordered proteins: physiological consequences",
pages = "156-111",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2928"
}

Vidović, M.,& Milić Komić, S.. (2021). Regulation of proteolysis of intrinsically disordered proteins: physiological consequences. in A closer look at proteolysis, 111-156.
https://hdl.handle.net/21.15107/rcub_rimsi_2928

Vidović M, Milić Komić S. Regulation of proteolysis of intrinsically disordered proteins: physiological consequences. in A closer look at proteolysis. 2021;:111-156.
https://hdl.handle.net/21.15107/rcub_rimsi_2928 .

Vidović, Marija, Milić Komić, Sonja, "Regulation of proteolysis of intrinsically disordered proteins: physiological consequences" in A closer look at proteolysis (2021):111-156,
https://hdl.handle.net/21.15107/rcub_rimsi_2928 .

TY  - JOUR
AU  - Vidović, Marija
AU  - Franchin, Cinzia
AU  - Morina, Filis
AU  - Veljović-Jovanović, Sonja
AU  - Masi, Antonio
AU  - Arrigoni, Giorgio
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1322
AB  - Resurrection plantRamonda serbicais a suitable model to investigate vegetative desiccation tolerance. However, the detailed study of these mechanisms at the protein level is hampered by the severe tissue water loss, high amount of phenolics and polysaccharide, and possible protein modifications and aggregations during the extraction and purification steps. When applied toR.serbicaleaves, widely used protein extraction protocols containing polyvinylpolypyrrolidone and ascorbate, as well as the phenol/SDS/buffer-based protocol recommended for recalcitrant plant tissues failed to eliminate persistent contamination and ensure high protein quality. Here we compared three protein extraction approaches aiming to establish the optimal one for both hydrated and desiccatedR.serbicaleaves. To evaluate the efficacy of these protocols by shotgun proteomics, we also created the firstR.serbicaannotated transcriptome database, available at. The detergent-free phenol-based extraction combined with dodecyl-beta-D-maltoside-assisted extraction enabled high-yield and high-purity protein extracts. The phenol-based protocol improved the protein-band resolution, band number, and intensity upon electrophoresis, and increased the protein yield and the number of identified peptides and protein groups by LC-MS/MS. Additionally, dodecyl-beta-D-maltoside enabled solubilisation and identification of more membrane-associated proteins. The presented study paves the way for investigating the desiccation tolerance inR.serbica, and we recommend this protocol for similar recalcitrant plant material.
PB  - Springer Heidelberg, Heidelberg
T2  - Analytical and Bioanalytical Chemistry
T1  - Efficient protein extraction for shotgun proteomics from hydrated and desiccated leaves of resurrection Ramonda serbica plants
EP  - 8312
IS  - 30
SP  - 8299
VL  - 412
DO  - 10.1007/s00216-020-02965-2
ER  - 

@article{
author = "Vidović, Marija and Franchin, Cinzia and Morina, Filis and Veljović-Jovanović, Sonja and Masi, Antonio and Arrigoni, Giorgio",
year = "2020",
abstract = "Resurrection plantRamonda serbicais a suitable model to investigate vegetative desiccation tolerance. However, the detailed study of these mechanisms at the protein level is hampered by the severe tissue water loss, high amount of phenolics and polysaccharide, and possible protein modifications and aggregations during the extraction and purification steps. When applied toR.serbicaleaves, widely used protein extraction protocols containing polyvinylpolypyrrolidone and ascorbate, as well as the phenol/SDS/buffer-based protocol recommended for recalcitrant plant tissues failed to eliminate persistent contamination and ensure high protein quality. Here we compared three protein extraction approaches aiming to establish the optimal one for both hydrated and desiccatedR.serbicaleaves. To evaluate the efficacy of these protocols by shotgun proteomics, we also created the firstR.serbicaannotated transcriptome database, available at. The detergent-free phenol-based extraction combined with dodecyl-beta-D-maltoside-assisted extraction enabled high-yield and high-purity protein extracts. The phenol-based protocol improved the protein-band resolution, band number, and intensity upon electrophoresis, and increased the protein yield and the number of identified peptides and protein groups by LC-MS/MS. Additionally, dodecyl-beta-D-maltoside enabled solubilisation and identification of more membrane-associated proteins. The presented study paves the way for investigating the desiccation tolerance inR.serbica, and we recommend this protocol for similar recalcitrant plant material.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Analytical and Bioanalytical Chemistry",
title = "Efficient protein extraction for shotgun proteomics from hydrated and desiccated leaves of resurrection Ramonda serbica plants",
pages = "8312-8299",
number = "30",
volume = "412",
doi = "10.1007/s00216-020-02965-2"
}

Vidović, M., Franchin, C., Morina, F., Veljović-Jovanović, S., Masi, A.,& Arrigoni, G.. (2020). Efficient protein extraction for shotgun proteomics from hydrated and desiccated leaves of resurrection Ramonda serbica plants. in Analytical and Bioanalytical Chemistry
Springer Heidelberg, Heidelberg., 412(30), 8299-8312.
https://doi.org/10.1007/s00216-020-02965-2

Vidović M, Franchin C, Morina F, Veljović-Jovanović S, Masi A, Arrigoni G. Efficient protein extraction for shotgun proteomics from hydrated and desiccated leaves of resurrection Ramonda serbica plants. in Analytical and Bioanalytical Chemistry. 2020;412(30):8299-8312.
doi:10.1007/s00216-020-02965-2 .

Vidović, Marija, Franchin, Cinzia, Morina, Filis, Veljović-Jovanović, Sonja, Masi, Antonio, Arrigoni, Giorgio, "Efficient protein extraction for shotgun proteomics from hydrated and desiccated leaves of resurrection Ramonda serbica plants" in Analytical and Bioanalytical Chemistry, 412, no. 30 (2020):8299-8312,
https://doi.org/10.1007/s00216-020-02965-2 . .

TY  - JOUR
AU  - Živanović, Bojana
AU  - Milić Komić, Sonja
AU  - Tosti, Tomislav
AU  - Vidović, Marija
AU  - Prokic, Ljiljana
AU  - Veljović-Jovanović, Sonja
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1347
AB  - Water deficit has a global impact on plant growth and crop yield. Climate changes are going to increase the intensity, duration and frequency of severe droughts, particularly in southern and south-eastern Europe, elevating the water scarcity issues. We aimed to assess the contribution of endogenous abscisic acid (ABA) in the protective mechanisms against water deficit, including stomatal conductance, relative water potential and the accumulation of osmoprotectants, as well as on growth parameters. To achieve that, we used a suitable model system, ABA-deficient tomato mutant, flacca and its parental line. Flacca mutant exhibited constitutively higher levels of soluble sugars (e.g., galactose, arabinose, sorbitol) and free amino acids (AAs) compared with the wild type (WT). Water deficit provoked the strong accumulation of proline in both genotypes, and total soluble sugars only in flacca. Upon re-watering, these osmolytes returned to the initial levels in both genotypes. Our results indicate that flacca compensated higher stomatal conductance with a higher constitutive level of free sugars and AAs. Additionally, we suggest that the accumulation of AAs, particularly proline and its precursors and specific branched-chain AAs in both, glucose and sucrose in flacca, and sorbitol in WT, could contribute to maintaining growth rate during water deficit and recovery in both tomato genotypes.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato
IS  - 9
VL  - 9
DO  - 10.3390/plants9091147
ER  - 

@article{
author = "Živanović, Bojana and Milić Komić, Sonja and Tosti, Tomislav and Vidović, Marija and Prokic, Ljiljana and Veljović-Jovanović, Sonja",
year = "2020",
abstract = "Water deficit has a global impact on plant growth and crop yield. Climate changes are going to increase the intensity, duration and frequency of severe droughts, particularly in southern and south-eastern Europe, elevating the water scarcity issues. We aimed to assess the contribution of endogenous abscisic acid (ABA) in the protective mechanisms against water deficit, including stomatal conductance, relative water potential and the accumulation of osmoprotectants, as well as on growth parameters. To achieve that, we used a suitable model system, ABA-deficient tomato mutant, flacca and its parental line. Flacca mutant exhibited constitutively higher levels of soluble sugars (e.g., galactose, arabinose, sorbitol) and free amino acids (AAs) compared with the wild type (WT). Water deficit provoked the strong accumulation of proline in both genotypes, and total soluble sugars only in flacca. Upon re-watering, these osmolytes returned to the initial levels in both genotypes. Our results indicate that flacca compensated higher stomatal conductance with a higher constitutive level of free sugars and AAs. Additionally, we suggest that the accumulation of AAs, particularly proline and its precursors and specific branched-chain AAs in both, glucose and sucrose in flacca, and sorbitol in WT, could contribute to maintaining growth rate during water deficit and recovery in both tomato genotypes.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato",
number = "9",
volume = "9",
doi = "10.3390/plants9091147"
}

Živanović, B., Milić Komić, S., Tosti, T., Vidović, M., Prokic, L.,& Veljović-Jovanović, S.. (2020). Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato. in Plants-Basel
MDPI, Basel., 9(9).
https://doi.org/10.3390/plants9091147

Živanović B, Milić Komić S, Tosti T, Vidović M, Prokic L, Veljović-Jovanović S. Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato. in Plants-Basel. 2020;9(9).
doi:10.3390/plants9091147 .

Živanović, Bojana, Milić Komić, Sonja, Tosti, Tomislav, Vidović, Marija, Prokic, Ljiljana, Veljović-Jovanović, Sonja, "Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato" in Plants-Basel, 9, no. 9 (2020),
https://doi.org/10.3390/plants9091147 . .

TY  - JOUR
AU  - Vidović, Marija
AU  - Cuković, Katarina
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1339
AB  - Resurrection plant Ramonda serbica is a suitable model to investigate mechanisms of desiccation tolerance, while variegated Pelargonium zonale has been proven to serve as an excellent model for the metabolite allocation between sink tissue and source tissue within the same organ. However, the genomes of these plants are still not sequenced, limiting their application in molecular studies. To investigate the transcript abundance by next-generation sequencing, high-quality RNA input is required. Leaves of both P. zonale and R. serbica are rich in polyphenols that interfere with high-quality RNA extraction by common protocols. Moreover, low water content and high amount of sugars and other osmoprotectants in desiccated R. serbica leaves present the additional challenge in total RNA extraction. Here, we evaluated and compared several already established TRIzol- and CTAB-based protocols aiming to develop the efficient, simple and low-cost methods for the extraction of the satisfactory yield RNA of great purity and integrity, required for the construction of high-quality cDNA libraries. Our results show that the CTAB-based protocol (i.e. CTAB 1b) enabled the extraction of high-quality RNA from photosynthetically active and non-photosynthetically active leaf sectors of P. zonale, with high RIN values. On the other hand, TRIzol-based protocol provided a high RNA yield with low contamination and high RNA integrity even in desiccated leaves of R. serbica. We envisage that the proposed protocol would be suitable for the RNA extractions from other desiccated organs (e.g. seeds, grains, pollen grains).
PB  - Springer Heidelberg, Heidelberg
T2  - 3 Biotech
T1  - Isolation of high-quality RNA from recalcitrant leaves of variegated and resurrection plants
IS  - 6
VL  - 10
DO  - 10.1007/s13205-020-02279-1
ER  - 

@article{
author = "Vidović, Marija and Cuković, Katarina",
year = "2020",
abstract = "Resurrection plant Ramonda serbica is a suitable model to investigate mechanisms of desiccation tolerance, while variegated Pelargonium zonale has been proven to serve as an excellent model for the metabolite allocation between sink tissue and source tissue within the same organ. However, the genomes of these plants are still not sequenced, limiting their application in molecular studies. To investigate the transcript abundance by next-generation sequencing, high-quality RNA input is required. Leaves of both P. zonale and R. serbica are rich in polyphenols that interfere with high-quality RNA extraction by common protocols. Moreover, low water content and high amount of sugars and other osmoprotectants in desiccated R. serbica leaves present the additional challenge in total RNA extraction. Here, we evaluated and compared several already established TRIzol- and CTAB-based protocols aiming to develop the efficient, simple and low-cost methods for the extraction of the satisfactory yield RNA of great purity and integrity, required for the construction of high-quality cDNA libraries. Our results show that the CTAB-based protocol (i.e. CTAB 1b) enabled the extraction of high-quality RNA from photosynthetically active and non-photosynthetically active leaf sectors of P. zonale, with high RIN values. On the other hand, TRIzol-based protocol provided a high RNA yield with low contamination and high RNA integrity even in desiccated leaves of R. serbica. We envisage that the proposed protocol would be suitable for the RNA extractions from other desiccated organs (e.g. seeds, grains, pollen grains).",
publisher = "Springer Heidelberg, Heidelberg",
journal = "3 Biotech",
title = "Isolation of high-quality RNA from recalcitrant leaves of variegated and resurrection plants",
number = "6",
volume = "10",
doi = "10.1007/s13205-020-02279-1"
}

Vidović, M.,& Cuković, K.. (2020). Isolation of high-quality RNA from recalcitrant leaves of variegated and resurrection plants. in 3 Biotech
Springer Heidelberg, Heidelberg., 10(6).
https://doi.org/10.1007/s13205-020-02279-1

Vidović M, Cuković K. Isolation of high-quality RNA from recalcitrant leaves of variegated and resurrection plants. in 3 Biotech. 2020;10(6).
doi:10.1007/s13205-020-02279-1 .

Vidović, Marija, Cuković, Katarina, "Isolation of high-quality RNA from recalcitrant leaves of variegated and resurrection plants" in 3 Biotech, 10, no. 6 (2020),
https://doi.org/10.1007/s13205-020-02279-1 . .

TY  - JOUR
AU  - Zang, Lili
AU  - Morere-Le, Paven, Marie-Christine
AU  - Clochard, Thibault
AU  - Porcher, Alexis
AU  - Satour, Pascale
AU  - Mojović, Miloš
AU  - Vidović, Marija
AU  - Limami, Anis M.
AU  - Montrichard, Francoise
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1300
AB  - In Medicago truncatula, nitrate, acting as a signal perceived by NITRATE TRANSPORTER1/PEPTIDE TRANSPORTER FAMILY 6.8 (MtNPF6.8), inhibits primary root growth through a reduction of root cell elongation. Since reactive oxygen species (ROS) produced and converted in root tip (O-2(center dot-) -> H2O2 -> (OH)-O-center dot) have been reported to control cell elongation, the impact of nitrate on the distribution of these ROS in the primary root of M. truncatula was analyzed. We found that nitrate reduced the content of O-2(center dot-), H2O2 and (OH)-O-center dot in the root tip of three wild type genotypes sensitive to nitrate (R108, DZA, A17), inhibition of root growth and O-2(center dot-) accumulation being highly correlated. Nitrate also modified the capacity of R108 root tip to produce or remove ROS. The ROS content decrease observed in R108 in response to nitrate is linked to changes in peroxidase activity (EC1.11.1.7) with an increase in peroxidative activity that scavenge H2O2 and a decrease in hydroxylic activity that converts H2O2 into (OH)-O-center dot. These changes impair the accumulation of H2O2 and then the accumulation of (OH)-O-center dot, the species responsible for cell wall loosening and cell elongation. Accordingly, nitrate inhibitory effect was abolished by externally added H2O2 or mimicked by KI, an H2O2 scavenger. In contrast, nitrate has no effect on ROS production or removal capacities in npf6.8-2, a knockdown line insensitive to nitrate, affected in the nitrate transporter MtNPF6.8 (in R108 background) by RNAi. Altogether, our data show that ROS are mediators acting downstream of MtNPF6.8 in the nitrate signaling pathway.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - Plant Physiology and Biochemistry
T1  - Nitrate inhibits primary root growth by reducing accumulation of reactive oxygen species in the root tip in Medicago truncatula
EP  - 373
SP  - 363
VL  - 146
DO  - 10.1016/j.plaphy.2019.11.006
ER  - 

@article{
author = "Zang, Lili and Morere-Le, Paven, Marie-Christine and Clochard, Thibault and Porcher, Alexis and Satour, Pascale and Mojović, Miloš and Vidović, Marija and Limami, Anis M. and Montrichard, Francoise",
year = "2020",
abstract = "In Medicago truncatula, nitrate, acting as a signal perceived by NITRATE TRANSPORTER1/PEPTIDE TRANSPORTER FAMILY 6.8 (MtNPF6.8), inhibits primary root growth through a reduction of root cell elongation. Since reactive oxygen species (ROS) produced and converted in root tip (O-2(center dot-) -> H2O2 -> (OH)-O-center dot) have been reported to control cell elongation, the impact of nitrate on the distribution of these ROS in the primary root of M. truncatula was analyzed. We found that nitrate reduced the content of O-2(center dot-), H2O2 and (OH)-O-center dot in the root tip of three wild type genotypes sensitive to nitrate (R108, DZA, A17), inhibition of root growth and O-2(center dot-) accumulation being highly correlated. Nitrate also modified the capacity of R108 root tip to produce or remove ROS. The ROS content decrease observed in R108 in response to nitrate is linked to changes in peroxidase activity (EC1.11.1.7) with an increase in peroxidative activity that scavenge H2O2 and a decrease in hydroxylic activity that converts H2O2 into (OH)-O-center dot. These changes impair the accumulation of H2O2 and then the accumulation of (OH)-O-center dot, the species responsible for cell wall loosening and cell elongation. Accordingly, nitrate inhibitory effect was abolished by externally added H2O2 or mimicked by KI, an H2O2 scavenger. In contrast, nitrate has no effect on ROS production or removal capacities in npf6.8-2, a knockdown line insensitive to nitrate, affected in the nitrate transporter MtNPF6.8 (in R108 background) by RNAi. Altogether, our data show that ROS are mediators acting downstream of MtNPF6.8 in the nitrate signaling pathway.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "Plant Physiology and Biochemistry",
title = "Nitrate inhibits primary root growth by reducing accumulation of reactive oxygen species in the root tip in Medicago truncatula",
pages = "373-363",
volume = "146",
doi = "10.1016/j.plaphy.2019.11.006"
}

Zang, L., Morere-Le, P. M., Clochard, T., Porcher, A., Satour, P., Mojović, M., Vidović, M., Limami, A. M.,& Montrichard, F.. (2020). Nitrate inhibits primary root growth by reducing accumulation of reactive oxygen species in the root tip in Medicago truncatula. in Plant Physiology and Biochemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 146, 363-373.
https://doi.org/10.1016/j.plaphy.2019.11.006

Zang L, Morere-Le PM, Clochard T, Porcher A, Satour P, Mojović M, Vidović M, Limami AM, Montrichard F. Nitrate inhibits primary root growth by reducing accumulation of reactive oxygen species in the root tip in Medicago truncatula. in Plant Physiology and Biochemistry. 2020;146:363-373.
doi:10.1016/j.plaphy.2019.11.006 .

Zang, Lili, Morere-Le, Paven, Marie-Christine, Clochard, Thibault, Porcher, Alexis, Satour, Pascale, Mojović, Miloš, Vidović, Marija, Limami, Anis M., Montrichard, Francoise, "Nitrate inhibits primary root growth by reducing accumulation of reactive oxygen species in the root tip in Medicago truncatula" in Plant Physiology and Biochemistry, 146 (2020):363-373,
https://doi.org/10.1016/j.plaphy.2019.11.006 . .

TY  - CONF
AU  - Vidović, Marija
AU  - Morina, Filis
AU  - Milić Komić, Sonja
AU  - Veljović-Jovanović, Sonja
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3090
AB  - Balkanic endemic plant species, Ramonda serbica, belongs to a small group of resurrection plants, since it is able to survive in an almost completely dehydrated state for months, and to fully recover metabolic functions upon watering. During drought and rehydration, reactive oxygen species increase presents an additional treat. In accordance, the increase of activities of the antioxidative enzymes, such as several superoxide dismutases and polyphenol oxidases in the critical first few hours of rehydration of R. serbica leaves was reported [1,2]. In fully hydrated leaves, the most abundant soluble phenolics were hydroxybenzoic acids (HBAs: p‐hydroxybenzoic, protocatechuic, and syringic acid) and catechin. Among hydroxycinnamic acids (HCAs), caffeic acid was the major one, beside ferulic and chlorogenic acids. In addition, four flavonoids: apigenin, luteolin, cyanidin and delphinidin were identified as glycosides. Although R. serbica settles exclusively north-facing steep rocky sides, in the shade of the surrounding forest canopy, with reduced solar irradiance, 5-d-exposure to supplemental UV-B radiation significantly enhanced amounts of HBA derivatives and anthocyanins (2.6 and 5.7 folds, respectively), whereas flavon glycosides and most of HCA derivatives were unaffected. The physiological aspect of this UV-B stimulative effect, as well as the role of phenolic metabolism in cellular desiccation survival has to be explained.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighbouring Regions, 2019,137
T1  - Phenolic compounds are involved in desiccation tolerance of endemic resurrection species Ramonda serbica Panc.
SP  - 137
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3090
ER  - 

@conference{
author = "Vidović, Marija and Morina, Filis and Milić Komić, Sonja and Veljović-Jovanović, Sonja",
year = "2019",
abstract = "Balkanic endemic plant species, Ramonda serbica, belongs to a small group of resurrection plants, since it is able to survive in an almost completely dehydrated state for months, and to fully recover metabolic functions upon watering. During drought and rehydration, reactive oxygen species increase presents an additional treat. In accordance, the increase of activities of the antioxidative enzymes, such as several superoxide dismutases and polyphenol oxidases in the critical first few hours of rehydration of R. serbica leaves was reported [1,2]. In fully hydrated leaves, the most abundant soluble phenolics were hydroxybenzoic acids (HBAs: p‐hydroxybenzoic, protocatechuic, and syringic acid) and catechin. Among hydroxycinnamic acids (HCAs), caffeic acid was the major one, beside ferulic and chlorogenic acids. In addition, four flavonoids: apigenin, luteolin, cyanidin and delphinidin were identified as glycosides. Although R. serbica settles exclusively north-facing steep rocky sides, in the shade of the surrounding forest canopy, with reduced solar irradiance, 5-d-exposure to supplemental UV-B radiation significantly enhanced amounts of HBA derivatives and anthocyanins (2.6 and 5.7 folds, respectively), whereas flavon glycosides and most of HCA derivatives were unaffected. The physiological aspect of this UV-B stimulative effect, as well as the role of phenolic metabolism in cellular desiccation survival has to be explained.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighbouring Regions, 2019,137",
title = "Phenolic compounds are involved in desiccation tolerance of endemic resurrection species Ramonda serbica Panc.",
pages = "137",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3090"
}

Vidović, M., Morina, F., Milić Komić, S.,& Veljović-Jovanović, S.. (2019). Phenolic compounds are involved in desiccation tolerance of endemic resurrection species Ramonda serbica Panc.. in 13th Symposium on the Flora of Southeastern Serbia and Neighbouring Regions, 2019,137
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 137.
https://hdl.handle.net/21.15107/rcub_rimsi_3090

Vidović M, Morina F, Milić Komić S, Veljović-Jovanović S. Phenolic compounds are involved in desiccation tolerance of endemic resurrection species Ramonda serbica Panc.. in 13th Symposium on the Flora of Southeastern Serbia and Neighbouring Regions, 2019,137. 2019;:137.
https://hdl.handle.net/21.15107/rcub_rimsi_3090 .

Vidović, Marija, Morina, Filis, Milić Komić, Sonja, Veljović-Jovanović, Sonja, "Phenolic compounds are involved in desiccation tolerance of endemic resurrection species Ramonda serbica Panc." in 13th Symposium on the Flora of Southeastern Serbia and Neighbouring Regions, 2019,137 (2019):137,
https://hdl.handle.net/21.15107/rcub_rimsi_3090 .

TY  - CHAP
AU  - Veljović-Jovanović, Sonja
AU  - Kukavica, Biljana
AU  - Vidović, Marija
AU  - Morina, Filis
AU  - Menckhoff, Ljiljana
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1185
AB  - Class III peroxidases (POXs; EC. 1.11.1.7), are secretory, multifunctional plant enzymes that catalyze the oxidation of a variety of substrates by hydrogen peroxide (H2O2). They show a remarkable diversity of isoenzymes, are encoded by a large number of paralogous genes, and are involved in a broad range of metabolic processes throughout plant growth and development. Peroxidases isoenzymes are located in the cell wall, apoplast and vacuole, and may be either soluble or ionically and covalently cell wall bound. They are involved in cell wall cross-linking and loosening, lignification and suberization, auxin catabolism and secondary metabolism. Due to their ability to control the levels of reactive oxygen species (ROS), POXs are efficient components of the antioxidative system induced in response to environmental stress, such as pathogen attack, metal excess, salinity, drought and high light intensity. In addition to the peroxidative function, POXs can catalyze H2O2 production in the oxidative cycle. Peroxidases are responsible either for cell elongation or cell wall stiffening, affecting carbon allocation, auxin level and redox homeostasis, which implicates their key role as being in the regulation of growth and defence under stress condition. This chapter will discuss novel insights into the functions of PODs with special emphasis on their localization, substrate specificity and the regulation of redox homeostasis.
PB  - Springer International Publishing
T2  - Antioxidants and Antioxidant Enzymes in Higher Plants
T1  - Class III peroxidases: Functions, localization and redox regulation of isoenzymes
EP  - 300
SP  - 269
DO  - 10.1007/978-3-319-75088-0_13
ER  - 

@inbook{
author = "Veljović-Jovanović, Sonja and Kukavica, Biljana and Vidović, Marija and Morina, Filis and Menckhoff, Ljiljana",
year = "2018",
abstract = "Class III peroxidases (POXs; EC. 1.11.1.7), are secretory, multifunctional plant enzymes that catalyze the oxidation of a variety of substrates by hydrogen peroxide (H2O2). They show a remarkable diversity of isoenzymes, are encoded by a large number of paralogous genes, and are involved in a broad range of metabolic processes throughout plant growth and development. Peroxidases isoenzymes are located in the cell wall, apoplast and vacuole, and may be either soluble or ionically and covalently cell wall bound. They are involved in cell wall cross-linking and loosening, lignification and suberization, auxin catabolism and secondary metabolism. Due to their ability to control the levels of reactive oxygen species (ROS), POXs are efficient components of the antioxidative system induced in response to environmental stress, such as pathogen attack, metal excess, salinity, drought and high light intensity. In addition to the peroxidative function, POXs can catalyze H2O2 production in the oxidative cycle. Peroxidases are responsible either for cell elongation or cell wall stiffening, affecting carbon allocation, auxin level and redox homeostasis, which implicates their key role as being in the regulation of growth and defence under stress condition. This chapter will discuss novel insights into the functions of PODs with special emphasis on their localization, substrate specificity and the regulation of redox homeostasis.",
publisher = "Springer International Publishing",
journal = "Antioxidants and Antioxidant Enzymes in Higher Plants",
booktitle = "Class III peroxidases: Functions, localization and redox regulation of isoenzymes",
pages = "300-269",
doi = "10.1007/978-3-319-75088-0_13"
}

Veljović-Jovanović, S., Kukavica, B., Vidović, M., Morina, F.,& Menckhoff, L.. (2018). Class III peroxidases: Functions, localization and redox regulation of isoenzymes. in Antioxidants and Antioxidant Enzymes in Higher Plants
Springer International Publishing., 269-300.
https://doi.org/10.1007/978-3-319-75088-0_13

Veljović-Jovanović S, Kukavica B, Vidović M, Morina F, Menckhoff L. Class III peroxidases: Functions, localization and redox regulation of isoenzymes. in Antioxidants and Antioxidant Enzymes in Higher Plants. 2018;:269-300.
doi:10.1007/978-3-319-75088-0_13 .

Veljović-Jovanović, Sonja, Kukavica, Biljana, Vidović, Marija, Morina, Filis, Menckhoff, Ljiljana, "Class III peroxidases: Functions, localization and redox regulation of isoenzymes" in Antioxidants and Antioxidant Enzymes in Higher Plants (2018):269-300,
https://doi.org/10.1007/978-3-319-75088-0_13 . .

TY  - CHAP
AU  - Veljović-Jovanović, Sonja
AU  - Vidović, Marija
AU  - Morina, Filis
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1178
AB  - During their lifespan, plants are frequently exposed to adverse environmental conditions such as high solar irradiance, drought, heat, chilling, salinity, metal excess, and nutrient deficiency. The effects of these factors on plants are often interrelated and usually result in a decreased capacity of carbon fixation in photosynthesis, disturbed redox homeostasis, and growth arrest. Under severe conditions, increased excitation pressure in the chloroplasts exceeds the antioxidative capacity of plant cells leading to oxidative damage of cellular constituents. Although the plant ascorbate (Asc) level varies depending on external factors, developmental stage, diurnal rhythm, and light, its redox status is related to redox homeostasis in the cell. In chloroplasts, peroxisomes, and cytosol, Asc has a key role in hydrogen peroxide (H2O2) scavenging via Asc peroxidase and is efficiently recycled via the ascorbate-glutathione (Asc-GSH) cycle and directly by monodehydroascorbate reductase activity. In apoplast and vacuoles, Asc is the main reductant of phenolic radicals generated under oxidative stress. Besides its antioxidative role, Asc has an important role in a complex and well-orchestrated plant response network to environmental stress, performing multiple tasks in redox signalling, regulation of enzymatic activities, modulation of gene expression, biosynthesis of phytohormones, and growth regulation. The content of Asc and its redox state is tightly related to cellular compartments. Therefore, it is important to emphasize Asc cellular distribution, which has a great impact on reactive oxygen species regulation and signalling. Numerous studies on transgenic plants with altered endogenous Asc levels and redox status were done with the aim to influence plant growth and improve tolerance to various abiotic stressors. In this chapter, we discuss the current understanding of the involvement of Asc metabolism in abiotic stress response. Moreover, the improved resilience to stressors in transgenic plants with altered enzymes involved in Asc biosynthesis and recycling will be discussed.
PB  - Springer International Publishing
T2  - Ascorbic Acid in Plant Growth, Development and Stress Tolerance
T1  - Ascorbate as a key player in plant abiotic stress response and tolerance
EP  - 109
SP  - 47
DO  - 10.1007/978-3-319-74057-7_3
ER  - 

@inbook{
author = "Veljović-Jovanović, Sonja and Vidović, Marija and Morina, Filis",
year = "2018",
abstract = "During their lifespan, plants are frequently exposed to adverse environmental conditions such as high solar irradiance, drought, heat, chilling, salinity, metal excess, and nutrient deficiency. The effects of these factors on plants are often interrelated and usually result in a decreased capacity of carbon fixation in photosynthesis, disturbed redox homeostasis, and growth arrest. Under severe conditions, increased excitation pressure in the chloroplasts exceeds the antioxidative capacity of plant cells leading to oxidative damage of cellular constituents. Although the plant ascorbate (Asc) level varies depending on external factors, developmental stage, diurnal rhythm, and light, its redox status is related to redox homeostasis in the cell. In chloroplasts, peroxisomes, and cytosol, Asc has a key role in hydrogen peroxide (H2O2) scavenging via Asc peroxidase and is efficiently recycled via the ascorbate-glutathione (Asc-GSH) cycle and directly by monodehydroascorbate reductase activity. In apoplast and vacuoles, Asc is the main reductant of phenolic radicals generated under oxidative stress. Besides its antioxidative role, Asc has an important role in a complex and well-orchestrated plant response network to environmental stress, performing multiple tasks in redox signalling, regulation of enzymatic activities, modulation of gene expression, biosynthesis of phytohormones, and growth regulation. The content of Asc and its redox state is tightly related to cellular compartments. Therefore, it is important to emphasize Asc cellular distribution, which has a great impact on reactive oxygen species regulation and signalling. Numerous studies on transgenic plants with altered endogenous Asc levels and redox status were done with the aim to influence plant growth and improve tolerance to various abiotic stressors. In this chapter, we discuss the current understanding of the involvement of Asc metabolism in abiotic stress response. Moreover, the improved resilience to stressors in transgenic plants with altered enzymes involved in Asc biosynthesis and recycling will be discussed.",
publisher = "Springer International Publishing",
journal = "Ascorbic Acid in Plant Growth, Development and Stress Tolerance",
booktitle = "Ascorbate as a key player in plant abiotic stress response and tolerance",
pages = "109-47",
doi = "10.1007/978-3-319-74057-7_3"
}

Veljović-Jovanović, S., Vidović, M.,& Morina, F.. (2018). Ascorbate as a key player in plant abiotic stress response and tolerance. in Ascorbic Acid in Plant Growth, Development and Stress Tolerance
Springer International Publishing., 47-109.
https://doi.org/10.1007/978-3-319-74057-7_3

Veljović-Jovanović S, Vidović M, Morina F. Ascorbate as a key player in plant abiotic stress response and tolerance. in Ascorbic Acid in Plant Growth, Development and Stress Tolerance. 2018;:47-109.
doi:10.1007/978-3-319-74057-7_3 .

Veljović-Jovanović, Sonja, Vidović, Marija, Morina, Filis, "Ascorbate as a key player in plant abiotic stress response and tolerance" in Ascorbic Acid in Plant Growth, Development and Stress Tolerance (2018):47-109,
https://doi.org/10.1007/978-3-319-74057-7_3 . .

TY  - JOUR
AU  - Morina, Filis
AU  - Vidović, Marija
AU  - Srećković, Tatjana
AU  - Radović, Vesela
AU  - Veljović-Jovanović, Sonja
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1089
AB  - We investigated metal accumulation in bamboo leaves during three seasons at three urban locations differing in pollution levels. The higher content of Cu, Pb, and Zn in the leaves was in correlation with the highest bioavailable content of these elements in the soil at the most polluted location. The content of leaf trace elements was higher in summer and autumn compared to spring. Scanning electron microscopy with energy dispersive X-ray spectroscopy showed that Si accumulation in bamboo leaves was the highest in epidermis and vascular tissue, and was co-localized with trace metals. Analysis of phytoliths showed co-deposition of Al, C, and Si, implying the involvement of Si in metal detoxification. Compared to a common urban tree, linden, bamboo showed better capacity to maintain cellular redox homeostasis under deteriorated environmental conditions. The results suggest that bamboo can be efficiently used for biomonitoring of air and soil metal pollution and remediation in urban areas.
PB  - Springer, New York
T2  - Bulletin of Environmental Contamination and Toxicology
T1  - Biomonitoring of Urban Pollution Using Silicon-Accumulating Species, Phyllostachys aureosulcata 'Aureocaulis'
EP  - 712
IS  - 6
SP  - 706
VL  - 99
DO  - 10.1007/s00128-017-2189-0
ER  - 

@article{
author = "Morina, Filis and Vidović, Marija and Srećković, Tatjana and Radović, Vesela and Veljović-Jovanović, Sonja",
year = "2017",
abstract = "We investigated metal accumulation in bamboo leaves during three seasons at three urban locations differing in pollution levels. The higher content of Cu, Pb, and Zn in the leaves was in correlation with the highest bioavailable content of these elements in the soil at the most polluted location. The content of leaf trace elements was higher in summer and autumn compared to spring. Scanning electron microscopy with energy dispersive X-ray spectroscopy showed that Si accumulation in bamboo leaves was the highest in epidermis and vascular tissue, and was co-localized with trace metals. Analysis of phytoliths showed co-deposition of Al, C, and Si, implying the involvement of Si in metal detoxification. Compared to a common urban tree, linden, bamboo showed better capacity to maintain cellular redox homeostasis under deteriorated environmental conditions. The results suggest that bamboo can be efficiently used for biomonitoring of air and soil metal pollution and remediation in urban areas.",
publisher = "Springer, New York",
journal = "Bulletin of Environmental Contamination and Toxicology",
title = "Biomonitoring of Urban Pollution Using Silicon-Accumulating Species, Phyllostachys aureosulcata 'Aureocaulis'",
pages = "712-706",
number = "6",
volume = "99",
doi = "10.1007/s00128-017-2189-0"
}

Morina, F., Vidović, M., Srećković, T., Radović, V.,& Veljović-Jovanović, S.. (2017). Biomonitoring of Urban Pollution Using Silicon-Accumulating Species, Phyllostachys aureosulcata 'Aureocaulis'. in Bulletin of Environmental Contamination and Toxicology
Springer, New York., 99(6), 706-712.
https://doi.org/10.1007/s00128-017-2189-0

Morina F, Vidović M, Srećković T, Radović V, Veljović-Jovanović S. Biomonitoring of Urban Pollution Using Silicon-Accumulating Species, Phyllostachys aureosulcata 'Aureocaulis'. in Bulletin of Environmental Contamination and Toxicology. 2017;99(6):706-712.
doi:10.1007/s00128-017-2189-0 .

Morina, Filis, Vidović, Marija, Srećković, Tatjana, Radović, Vesela, Veljović-Jovanović, Sonja, "Biomonitoring of Urban Pollution Using Silicon-Accumulating Species, Phyllostachys aureosulcata 'Aureocaulis'" in Bulletin of Environmental Contamination and Toxicology, 99, no. 6 (2017):706-712,
https://doi.org/10.1007/s00128-017-2189-0 . .