TY  - CONF
AU  - Milicevic, Katarina
AU  - Bataveljic, Danijela
AU  - Bogdanović Pristov, Jelena
AU  - Andjus, Pavle
AU  - Nikolic, Ljiljana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2014
AB  - The astrocytic network maintains homeostasis in the central nervous system (CNS) 
through interactions with neighboring cells. In the CNS autoimmune disease, multiple 
sclerosis (MS), neuroinflammatory conditions modulate these cell-to-cell interactions. 
Our previous work revealed that the immune cells infiltrated into the CNS (CNS-IICs) 
of experimental autoimmune encemphalomyelitis (EAE) rat, an animal model of MS, 
rapidly alter the activity pattern of astrocytes by activating the glial P2X7 receptor 
(P2X7R). In the present study we further defined the mechanisms responsible for 
astrocytes’ activation in the presence of CNS-IICs. For this purpose, we used an in 
vitro experimental setup and monitored Ca2+ dynamics in Fluo-4-labeled cultured 
naïve astrocytes following brief bath application of CNS-IICs isolated from the spinal 
cord of the EAE rat. Our data indicate that the astroglial αvβ3-integrin is involved in 
the initial contact of astrocytes with CNS-IICs, since blocking αvβ3-integrin reduced 
the expected astrocytic Ca2+ response. Furthermore, blocking of mitochondrial 
Na+/Ca2+- and H+/Ca2+- exchangers in astrocytes promoted an augmentation of the 
intracellular Ca2+ increase and a higher ATP release after brief exposure to CNS-IICs, 
demonstrating that mitochondria regulate the astrocyte-CNS IICs cell-cell interaction. 
Overall, our study expands the understanding of astrocytes’ interaction with 
autoreactive immune cells that are present in their local environment in an 
autoimmune disease. This offers a new conceptual framework for considering direct 
astrocyte–immune cell interaction to design new strategies for therapy development in 
the treatment of MS.
PB  - Serbian Neuroscience Society
C3  - 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS
T1  - αVβ3-Integrin and mitochondria mediate astrocyte response to  autoreactive immune cells
SP  - 101
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2014
ER  - 

@conference{
author = "Milicevic, Katarina and Bataveljic, Danijela and Bogdanović Pristov, Jelena and Andjus, Pavle and Nikolic, Ljiljana",
year = "2023",
abstract = "The astrocytic network maintains homeostasis in the central nervous system (CNS) 
through interactions with neighboring cells. In the CNS autoimmune disease, multiple 
sclerosis (MS), neuroinflammatory conditions modulate these cell-to-cell interactions. 
Our previous work revealed that the immune cells infiltrated into the CNS (CNS-IICs) 
of experimental autoimmune encemphalomyelitis (EAE) rat, an animal model of MS, 
rapidly alter the activity pattern of astrocytes by activating the glial P2X7 receptor 
(P2X7R). In the present study we further defined the mechanisms responsible for 
astrocytes’ activation in the presence of CNS-IICs. For this purpose, we used an in 
vitro experimental setup and monitored Ca2+ dynamics in Fluo-4-labeled cultured 
naïve astrocytes following brief bath application of CNS-IICs isolated from the spinal 
cord of the EAE rat. Our data indicate that the astroglial αvβ3-integrin is involved in 
the initial contact of astrocytes with CNS-IICs, since blocking αvβ3-integrin reduced 
the expected astrocytic Ca2+ response. Furthermore, blocking of mitochondrial 
Na+/Ca2+- and H+/Ca2+- exchangers in astrocytes promoted an augmentation of the 
intracellular Ca2+ increase and a higher ATP release after brief exposure to CNS-IICs, 
demonstrating that mitochondria regulate the astrocyte-CNS IICs cell-cell interaction. 
Overall, our study expands the understanding of astrocytes’ interaction with 
autoreactive immune cells that are present in their local environment in an 
autoimmune disease. This offers a new conceptual framework for considering direct 
astrocyte–immune cell interaction to design new strategies for therapy development in 
the treatment of MS.",
publisher = "Serbian Neuroscience Society",
journal = "8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS",
title = "αVβ3-Integrin and mitochondria mediate astrocyte response to  autoreactive immune cells",
pages = "101",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2014"
}

Milicevic, K., Bataveljic, D., Bogdanović Pristov, J., Andjus, P.,& Nikolic, L.. (2023). αVβ3-Integrin and mitochondria mediate astrocyte response to  autoreactive immune cells. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS
Serbian Neuroscience Society., 101.
https://hdl.handle.net/21.15107/rcub_rimsi_2014

Milicevic K, Bataveljic D, Bogdanović Pristov J, Andjus P, Nikolic L. αVβ3-Integrin and mitochondria mediate astrocyte response to  autoreactive immune cells. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS. 2023;:101.
https://hdl.handle.net/21.15107/rcub_rimsi_2014 .

Milicevic, Katarina, Bataveljic, Danijela, Bogdanović Pristov, Jelena, Andjus, Pavle, Nikolic, Ljiljana, "αVβ3-Integrin and mitochondria mediate astrocyte response to  autoreactive immune cells" in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS (2023):101,
https://hdl.handle.net/21.15107/rcub_rimsi_2014 .