TY  - JOUR
AU  - Peric, Mina
AU  - Bataveljic, Danijela
AU  - Bijelic, Dunja
AU  - Milicević, Katarina
AU  - Andjus, Pavle R.
AU  - Bogdanović Pristov, Jelena
AU  - Nikolić, Ljiljana M.
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1546
AB  - We describe an approach for studying the physiology of single live cells using the conceptionally novel upright microscope/patch-clamp configuration. Electrophysiology experiments typically require a microscope with the fixed stage position and the motion control of the microscope objective. Here, we demonstrate that a microscope with a z-axis movable stage and a fixed objective can also be efficiently used in combination with the patch-clamp technique. We define a set of underlying principles governing the operation of this microscope/patch-clamp configuration and demonstrate its performance in practice using cultured astrocytes, microglia, and oligodendrocytes. Experimental results show that our custom configuration provides stable recordings, has a high success rate of the whole-cell patch-clamp trials, can be effectively applied to study cellular physiology of glial cells, and provides comparable performance and usability to the commercially available systems. Our system can be easily replicated or adapted to suit the needs of the research groups and can be cost-effective in reducing the investments in purchasing additional equipment. We provide step-by-step instructions on implementing an upright microscope with z-axis movable stage as a routine workhorse for patch-clamping.
PB  - Wiley, Hoboken
T2  - Microscopy Research and Technique
T1  - Approach for patch-clamping using an upright microscope with z-axis movable stage
DO  - 10.1002/jemt.24066
ER  - 

@article{
author = "Peric, Mina and Bataveljic, Danijela and Bijelic, Dunja and Milicević, Katarina and Andjus, Pavle R. and Bogdanović Pristov, Jelena and Nikolić, Ljiljana M.",
year = "2022",
abstract = "We describe an approach for studying the physiology of single live cells using the conceptionally novel upright microscope/patch-clamp configuration. Electrophysiology experiments typically require a microscope with the fixed stage position and the motion control of the microscope objective. Here, we demonstrate that a microscope with a z-axis movable stage and a fixed objective can also be efficiently used in combination with the patch-clamp technique. We define a set of underlying principles governing the operation of this microscope/patch-clamp configuration and demonstrate its performance in practice using cultured astrocytes, microglia, and oligodendrocytes. Experimental results show that our custom configuration provides stable recordings, has a high success rate of the whole-cell patch-clamp trials, can be effectively applied to study cellular physiology of glial cells, and provides comparable performance and usability to the commercially available systems. Our system can be easily replicated or adapted to suit the needs of the research groups and can be cost-effective in reducing the investments in purchasing additional equipment. We provide step-by-step instructions on implementing an upright microscope with z-axis movable stage as a routine workhorse for patch-clamping.",
publisher = "Wiley, Hoboken",
journal = "Microscopy Research and Technique",
title = "Approach for patch-clamping using an upright microscope with z-axis movable stage",
doi = "10.1002/jemt.24066"
}

Peric, M., Bataveljic, D., Bijelic, D., Milicević, K., Andjus, P. R., Bogdanović Pristov, J.,& Nikolić, L. M.. (2022). Approach for patch-clamping using an upright microscope with z-axis movable stage. in Microscopy Research and Technique
Wiley, Hoboken..
https://doi.org/10.1002/jemt.24066

Peric M, Bataveljic D, Bijelic D, Milicević K, Andjus PR, Bogdanović Pristov J, Nikolić LM. Approach for patch-clamping using an upright microscope with z-axis movable stage. in Microscopy Research and Technique. 2022;.
doi:10.1002/jemt.24066 .

Peric, Mina, Bataveljic, Danijela, Bijelic, Dunja, Milicević, Katarina, Andjus, Pavle R., Bogdanović Pristov, Jelena, Nikolić, Ljiljana M., "Approach for patch-clamping using an upright microscope with z-axis movable stage" in Microscopy Research and Technique (2022),
https://doi.org/10.1002/jemt.24066 . .

TY  - JOUR
AU  - Bijelic, Dunja D.
AU  - Milicević, Katarina
AU  - Lazarević, Milica N.
AU  - Miljković, Đorđe
AU  - Bogdanović Pristov, Jelena
AU  - Savić, Danijela Z
AU  - Petković, Branka
AU  - Andjus, Pavle R.
AU  - Momcilović, Miljana
AU  - Nikolić, Ljiljana M.
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1317
AB  - Interaction between autoreactive immune cells and astroglia is an important part of the pathologic processes that fuel neurodegeneration in multiple sclerosis. In this inflammatory disease, immune cells enter into the central nervous system (CNS) and they spread through CNS parenchyma, but the impact of these autoreactive immune cells on the activity pattern of astrocytes has not been defined. By exploiting naive astrocytes in culture and CNS-infiltrated immune cells (CNS IICs) isolated from rat with experimental autoimmune encephalomyelitis (EAE), here we demonstrate previously unrecognized properties of immune cell-astrocyte interaction. We show that CNS IICs but not the peripheral immune cell application, evokes a rapid and vigorous intracellular Ca(2+)increase in astrocytes by promoting glial release of ATP. ATP propagated Ca(2+)elevation through glial purinergic P2X7 receptor activation by the hemichannel-dependent nucleotide release mechanism. Astrocyte Ca(2+)increase is specifically triggered by the autoreactive CD4(+)T-cell application and these two cell types exhibit close spatial interaction in EAE. Therefore, Ca(2+)signals may mediate a rapid astroglial response to the autoreactive immune cells in their local environment. This property of immune cell-astrocyte interaction may be important to consider in studies interrogating CNS autoimmune disease.
PB  - Wiley, Hoboken
T2  - Journal of Neuroscience Research
T1  - Central nervous system-infiltrated immune cells induce calcium increase in astrocytes via astroglial purinergic signaling
EP  - 2332
IS  - 11
SP  - 2317
VL  - 98
DO  - 10.1002/jnr.24699
ER  - 

@article{
author = "Bijelic, Dunja D. and Milicević, Katarina and Lazarević, Milica N. and Miljković, Đorđe and Bogdanović Pristov, Jelena and Savić, Danijela Z and Petković, Branka and Andjus, Pavle R. and Momcilović, Miljana and Nikolić, Ljiljana M.",
year = "2020",
abstract = "Interaction between autoreactive immune cells and astroglia is an important part of the pathologic processes that fuel neurodegeneration in multiple sclerosis. In this inflammatory disease, immune cells enter into the central nervous system (CNS) and they spread through CNS parenchyma, but the impact of these autoreactive immune cells on the activity pattern of astrocytes has not been defined. By exploiting naive astrocytes in culture and CNS-infiltrated immune cells (CNS IICs) isolated from rat with experimental autoimmune encephalomyelitis (EAE), here we demonstrate previously unrecognized properties of immune cell-astrocyte interaction. We show that CNS IICs but not the peripheral immune cell application, evokes a rapid and vigorous intracellular Ca(2+)increase in astrocytes by promoting glial release of ATP. ATP propagated Ca(2+)elevation through glial purinergic P2X7 receptor activation by the hemichannel-dependent nucleotide release mechanism. Astrocyte Ca(2+)increase is specifically triggered by the autoreactive CD4(+)T-cell application and these two cell types exhibit close spatial interaction in EAE. Therefore, Ca(2+)signals may mediate a rapid astroglial response to the autoreactive immune cells in their local environment. This property of immune cell-astrocyte interaction may be important to consider in studies interrogating CNS autoimmune disease.",
publisher = "Wiley, Hoboken",
journal = "Journal of Neuroscience Research",
title = "Central nervous system-infiltrated immune cells induce calcium increase in astrocytes via astroglial purinergic signaling",
pages = "2332-2317",
number = "11",
volume = "98",
doi = "10.1002/jnr.24699"
}

Bijelic, D. D., Milicević, K., Lazarević, M. N., Miljković, Đ., Bogdanović Pristov, J., Savić, D. Z., Petković, B., Andjus, P. R., Momcilović, M.,& Nikolić, L. M.. (2020). Central nervous system-infiltrated immune cells induce calcium increase in astrocytes via astroglial purinergic signaling. in Journal of Neuroscience Research
Wiley, Hoboken., 98(11), 2317-2332.
https://doi.org/10.1002/jnr.24699

Bijelic DD, Milicević K, Lazarević MN, Miljković Đ, Bogdanović Pristov J, Savić DZ, Petković B, Andjus PR, Momcilović M, Nikolić LM. Central nervous system-infiltrated immune cells induce calcium increase in astrocytes via astroglial purinergic signaling. in Journal of Neuroscience Research. 2020;98(11):2317-2332.
doi:10.1002/jnr.24699 .

Bijelic, Dunja D., Milicević, Katarina, Lazarević, Milica N., Miljković, Đorđe, Bogdanović Pristov, Jelena, Savić, Danijela Z, Petković, Branka, Andjus, Pavle R., Momcilović, Miljana, Nikolić, Ljiljana M., "Central nervous system-infiltrated immune cells induce calcium increase in astrocytes via astroglial purinergic signaling" in Journal of Neuroscience Research, 98, no. 11 (2020):2317-2332,
https://doi.org/10.1002/jnr.24699 . .