TY  - JOUR
AU  - Milicevic, Katarina
AU  - Bataveljic, Danijela
AU  - Bogdanović Pristov, Jelena
AU  - Andjus, Pavle
AU  - Nikolic, Ljiljana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2007
AB  - In multiple sclerosis (MS), glial cells astrocytes interact with the autoreactive immune cells that attack the central nervous system (CNS), which causes and sustains neuroinflammation. However, little is known about the direct interaction between these cells when they are in close proximity in the inflamed CNS. By using an experimental autoimmune encephalomyelitis (EAE) model of MS, we previously found that in the proximity of autoreactive CNS-infiltrated immune cells (CNS-IICs), astrocytes respond with a rapid calcium increase that is mediated by the autocrine P2X7 receptor (P2X7R) activation. We now reveal that the mechanisms regulating this direct interaction of astrocytes and CNS-IICs involve the coupling between P2X7R, connexin-43, and β3-integrin. We found that P2X7R and astroglial connexin-43 interact and concentrate in the immediate proximity of the CNS-IICs in EAE. P2X7R also interacts with β3-integrin, and the block of astroglial αvβ3-integrin reduces the P2X7R-dependent calcium response of astrocytes upon encountering CNS-IICs. This interaction was dependent on astroglial mitochondrial activity, which regulated the ATP-driven P2X7R activation and facilitated the termination of the astrocytic calcium response evoked by CNS-IICs. By further defining the interactions between the CNS and the immune system, our findings provide a novel perspective toward expanding integrin-targeting therapeutic approaches for MS treatment by controlling the cell–cell interactions between astrocytes and CNS-IICs.
PB  - MDPI
T2  - Cells
T1  - Astroglial Cell-to-Cell Interaction with Autoreactive Immune  Cells in Experimental Autoimmune Encephalomyelitis Involves  P2X7 Receptor, 3-Integrin, and Connexin-43
IS  - 13
SP  - 1786
VL  - 12
DO  - https://doi.org/10.3390/cells12131786
ER  - 

@article{
author = "Milicevic, Katarina and Bataveljic, Danijela and Bogdanović Pristov, Jelena and Andjus, Pavle and Nikolic, Ljiljana",
year = "2023",
abstract = "In multiple sclerosis (MS), glial cells astrocytes interact with the autoreactive immune cells that attack the central nervous system (CNS), which causes and sustains neuroinflammation. However, little is known about the direct interaction between these cells when they are in close proximity in the inflamed CNS. By using an experimental autoimmune encephalomyelitis (EAE) model of MS, we previously found that in the proximity of autoreactive CNS-infiltrated immune cells (CNS-IICs), astrocytes respond with a rapid calcium increase that is mediated by the autocrine P2X7 receptor (P2X7R) activation. We now reveal that the mechanisms regulating this direct interaction of astrocytes and CNS-IICs involve the coupling between P2X7R, connexin-43, and β3-integrin. We found that P2X7R and astroglial connexin-43 interact and concentrate in the immediate proximity of the CNS-IICs in EAE. P2X7R also interacts with β3-integrin, and the block of astroglial αvβ3-integrin reduces the P2X7R-dependent calcium response of astrocytes upon encountering CNS-IICs. This interaction was dependent on astroglial mitochondrial activity, which regulated the ATP-driven P2X7R activation and facilitated the termination of the astrocytic calcium response evoked by CNS-IICs. By further defining the interactions between the CNS and the immune system, our findings provide a novel perspective toward expanding integrin-targeting therapeutic approaches for MS treatment by controlling the cell–cell interactions between astrocytes and CNS-IICs.",
publisher = "MDPI",
journal = "Cells",
title = "Astroglial Cell-to-Cell Interaction with Autoreactive Immune  Cells in Experimental Autoimmune Encephalomyelitis Involves  P2X7 Receptor, 3-Integrin, and Connexin-43",
number = "13",
pages = "1786",
volume = "12",
doi = "https://doi.org/10.3390/cells12131786"
}

Milicevic, K., Bataveljic, D., Bogdanović Pristov, J., Andjus, P.,& Nikolic, L.. (2023). Astroglial Cell-to-Cell Interaction with Autoreactive Immune  Cells in Experimental Autoimmune Encephalomyelitis Involves  P2X7 Receptor, 3-Integrin, and Connexin-43. in Cells
MDPI., 12(13), 1786.
https://doi.org/https://doi.org/10.3390/cells12131786

Milicevic K, Bataveljic D, Bogdanović Pristov J, Andjus P, Nikolic L. Astroglial Cell-to-Cell Interaction with Autoreactive Immune  Cells in Experimental Autoimmune Encephalomyelitis Involves  P2X7 Receptor, 3-Integrin, and Connexin-43. in Cells. 2023;12(13):1786.
doi:https://doi.org/10.3390/cells12131786 .

Milicevic, Katarina, Bataveljic, Danijela, Bogdanović Pristov, Jelena, Andjus, Pavle, Nikolic, Ljiljana, "Astroglial Cell-to-Cell Interaction with Autoreactive Immune  Cells in Experimental Autoimmune Encephalomyelitis Involves  P2X7 Receptor, 3-Integrin, and Connexin-43" in Cells, 12, no. 13 (2023):1786,
https://doi.org/https://doi.org/10.3390/cells12131786 . .