TY  - CONF
AU  - Simonović, Jelena
AU  - Toljić, Boško
AU  - Rasković, Božidar
AU  - Jovanović, Vladimir P.
AU  - Lazarević, M.
AU  - Milošević, Milena
AU  - Nikolić, N.
AU  - Panajotović, R.
AU  - Milašin, J.
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1266
AB  - Aim To characterize stem cells originating from different dental tissues (apical papilla [SCAP], dental follicle [DFSC], and pulp [DPSC]) and test the capacity of Raman microspectroscopy to distinguish between the three dental stem cell types. Methods SCAR DFSC, and DPSC cultures were generated from three immature wisdom teeth originating from three patients. Cell stemness was confirmed by inducing neuro-, osteo-, chondro-, and adipo-differentiaton and by mesenchymal marker expression analysis by flow-cytometry and real-time polymerase chain reaction. Cellular components were then evaluated by Raman microspectroscopy. Results We found differences between SCAP, DFSC, and DPSC Raman spectra. The ratio between proteins and nucleic acids (748/770), a parameter for discriminating more differentiated from less differentiated cells, showed significant differences between the three cell types. All cells also displayed a fingerprint region in the 600-700 cm(-1) range, and characteristic lipid peaks at positions 1440 cm(-1) and 1650 cm(-1). Conclusion Although different dental stem cells exhibited similar Raman spectra, the method enabled us to make subtle distinction between them.
PB  - Medicinska Naklada Zagreb
C3  - Croatian Medical Journal
T1  - Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells
EP  - 86
IS  - 2
SP  - 78
VL  - 60
DO  - 10.3325/cmj.2019.60.78
ER  - 

@conference{
author = "Simonović, Jelena and Toljić, Boško and Rasković, Božidar and Jovanović, Vladimir P. and Lazarević, M. and Milošević, Milena and Nikolić, N. and Panajotović, R. and Milašin, J.",
year = "2019",
abstract = "Aim To characterize stem cells originating from different dental tissues (apical papilla [SCAP], dental follicle [DFSC], and pulp [DPSC]) and test the capacity of Raman microspectroscopy to distinguish between the three dental stem cell types. Methods SCAR DFSC, and DPSC cultures were generated from three immature wisdom teeth originating from three patients. Cell stemness was confirmed by inducing neuro-, osteo-, chondro-, and adipo-differentiaton and by mesenchymal marker expression analysis by flow-cytometry and real-time polymerase chain reaction. Cellular components were then evaluated by Raman microspectroscopy. Results We found differences between SCAP, DFSC, and DPSC Raman spectra. The ratio between proteins and nucleic acids (748/770), a parameter for discriminating more differentiated from less differentiated cells, showed significant differences between the three cell types. All cells also displayed a fingerprint region in the 600-700 cm(-1) range, and characteristic lipid peaks at positions 1440 cm(-1) and 1650 cm(-1). Conclusion Although different dental stem cells exhibited similar Raman spectra, the method enabled us to make subtle distinction between them.",
publisher = "Medicinska Naklada Zagreb",
journal = "Croatian Medical Journal",
title = "Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells",
pages = "86-78",
number = "2",
volume = "60",
doi = "10.3325/cmj.2019.60.78"
}

Simonović, J., Toljić, B., Rasković, B., Jovanović, V. P., Lazarević, M., Milošević, M., Nikolić, N., Panajotović, R.,& Milašin, J.. (2019). Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells. in Croatian Medical Journal
Medicinska Naklada Zagreb., 60(2), 78-86.
https://doi.org/10.3325/cmj.2019.60.78

Simonović J, Toljić B, Rasković B, Jovanović VP, Lazarević M, Milošević M, Nikolić N, Panajotović R, Milašin J. Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells. in Croatian Medical Journal. 2019;60(2):78-86.
doi:10.3325/cmj.2019.60.78 .

Simonović, Jelena, Toljić, Boško, Rasković, Božidar, Jovanović, Vladimir P., Lazarević, M., Milošević, Milena, Nikolić, N., Panajotović, R., Milašin, J., "Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells" in Croatian Medical Journal, 60, no. 2 (2019):78-86,
https://doi.org/10.3325/cmj.2019.60.78 . .

TY  - JOUR
AU  - Gačić, Zoran
AU  - Milošević, Milena
AU  - Mićković, Branislav
AU  - Nikčević, Miroslav
AU  - Damjanović, Ilija
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/915
AB  - Temperature dependence of electroretinogram (ERG) was investigated in 3 fish species occupying different habitats - dogfish shark (Scyliorhinus canicula), Prussian carp (Carassius gibelio) and European eel (Anguilla anguilla). Acute cooling of the shark isolated eyecup from 23 degrees C down to 6 degrees C induced suppression of the electroretinographic b-wave - a complete degradation of this component was observed at 6 degrees C. On the other hand, photoreceptor component of the ERG, the negative late receptor potential was not affected by cooling. The fact that the suppression of the dogfish shark b-wave at low temperatures was as a rule irreversible testifies about breakdown of neural retinal function at cold temperature extremes. Although in vivo experiments on immobilized Prussian carps have never resulted in complete deterioration of the b-wave at low temperatures, significant suppression of this ERG component by cooling was detected. Suppressing the effect of low temperatures on Prussian carp ERG might be due to the fact that C. gibelio, as well as other cyprinids, can be characterized as a warmwater species preferring temperatures well above cold extremes. The ERG of the eel, the third examined species, exhibited the strongest resistance to extremely low temperatures. During acute cooling of in situ eyecup preparations of migrating silver eels from 30 degrees C down to 2 degrees C the form of ERG became wider, but the amplitude of the b-wave only slightly decreased. High tolerance of eel b-wave to cold extremes shown in our study complies with ecological data confirming eurythermia in migrating silver eels remarkably adapted to cold-water environment as well.
PB  - Elsevier Science Inc, New York
T2  - Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology
T1  - Effects of acute cooling on fish electroretinogram: A comparative study
EP  - 155
SP  - 150
VL  - 184
DO  - 10.1016/j.cbpa.2015.03.002
ER  - 

@article{
author = "Gačić, Zoran and Milošević, Milena and Mićković, Branislav and Nikčević, Miroslav and Damjanović, Ilija",
year = "2015",
abstract = "Temperature dependence of electroretinogram (ERG) was investigated in 3 fish species occupying different habitats - dogfish shark (Scyliorhinus canicula), Prussian carp (Carassius gibelio) and European eel (Anguilla anguilla). Acute cooling of the shark isolated eyecup from 23 degrees C down to 6 degrees C induced suppression of the electroretinographic b-wave - a complete degradation of this component was observed at 6 degrees C. On the other hand, photoreceptor component of the ERG, the negative late receptor potential was not affected by cooling. The fact that the suppression of the dogfish shark b-wave at low temperatures was as a rule irreversible testifies about breakdown of neural retinal function at cold temperature extremes. Although in vivo experiments on immobilized Prussian carps have never resulted in complete deterioration of the b-wave at low temperatures, significant suppression of this ERG component by cooling was detected. Suppressing the effect of low temperatures on Prussian carp ERG might be due to the fact that C. gibelio, as well as other cyprinids, can be characterized as a warmwater species preferring temperatures well above cold extremes. The ERG of the eel, the third examined species, exhibited the strongest resistance to extremely low temperatures. During acute cooling of in situ eyecup preparations of migrating silver eels from 30 degrees C down to 2 degrees C the form of ERG became wider, but the amplitude of the b-wave only slightly decreased. High tolerance of eel b-wave to cold extremes shown in our study complies with ecological data confirming eurythermia in migrating silver eels remarkably adapted to cold-water environment as well.",
publisher = "Elsevier Science Inc, New York",
journal = "Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology",
title = "Effects of acute cooling on fish electroretinogram: A comparative study",
pages = "155-150",
volume = "184",
doi = "10.1016/j.cbpa.2015.03.002"
}

Gačić, Z., Milošević, M., Mićković, B., Nikčević, M.,& Damjanović, I.. (2015). Effects of acute cooling on fish electroretinogram: A comparative study. in Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology
Elsevier Science Inc, New York., 184, 150-155.
https://doi.org/10.1016/j.cbpa.2015.03.002

Gačić Z, Milošević M, Mićković B, Nikčević M, Damjanović I. Effects of acute cooling on fish electroretinogram: A comparative study. in Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology. 2015;184:150-155.
doi:10.1016/j.cbpa.2015.03.002 .

Gačić, Zoran, Milošević, Milena, Mićković, Branislav, Nikčević, Miroslav, Damjanović, Ilija, "Effects of acute cooling on fish electroretinogram: A comparative study" in Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology, 184 (2015):150-155,
https://doi.org/10.1016/j.cbpa.2015.03.002 . .

TY  - JOUR
AU  - Gačić, Zoran
AU  - Bajić, Aleksandar
AU  - Milošević, Milena
AU  - Nikčević, Miroslav
AU  - Mićković, Branislav
AU  - Hegediš, Aleksandar
AU  - Gacic, L.
AU  - Damjanović, Ilija
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/815
AB  - One of the purposes of this study was to examine whether b-wave measurements can be used in the evaluation of scotopic spectral sensitivity in Prussian carp measurements when the eyes were surgically deprived of cornea, lens, and most of the vitreous. Another goal was testing the new fitting procedure for A2-based photopigments. Using fitted amplitude-log intensity functions for threshold calculation, and two models for computer-assisted fitting of spectral sensitivity curves, no significant differences in lambda (max) were found between rod photopigments and b-wave-based spectral sensitivity.
PB  - Springer, Dordrecht
T2  - Fish Physiology and Biochemistry
T1  - Spectral sensitivity of the electroretinogram b-wave in dark-adapted Prussian carp (Carassius gibelio Bloch, 1782)
EP  - 1906
IS  - 6
SP  - 1899
VL  - 40
DO  - 10.1007/s10695-014-9977-9
ER  - 

@article{
author = "Gačić, Zoran and Bajić, Aleksandar and Milošević, Milena and Nikčević, Miroslav and Mićković, Branislav and Hegediš, Aleksandar and Gacic, L. and Damjanović, Ilija",
year = "2014",
abstract = "One of the purposes of this study was to examine whether b-wave measurements can be used in the evaluation of scotopic spectral sensitivity in Prussian carp measurements when the eyes were surgically deprived of cornea, lens, and most of the vitreous. Another goal was testing the new fitting procedure for A2-based photopigments. Using fitted amplitude-log intensity functions for threshold calculation, and two models for computer-assisted fitting of spectral sensitivity curves, no significant differences in lambda (max) were found between rod photopigments and b-wave-based spectral sensitivity.",
publisher = "Springer, Dordrecht",
journal = "Fish Physiology and Biochemistry",
title = "Spectral sensitivity of the electroretinogram b-wave in dark-adapted Prussian carp (Carassius gibelio Bloch, 1782)",
pages = "1906-1899",
number = "6",
volume = "40",
doi = "10.1007/s10695-014-9977-9"
}

Gačić, Z., Bajić, A., Milošević, M., Nikčević, M., Mićković, B., Hegediš, A., Gacic, L.,& Damjanović, I.. (2014). Spectral sensitivity of the electroretinogram b-wave in dark-adapted Prussian carp (Carassius gibelio Bloch, 1782). in Fish Physiology and Biochemistry
Springer, Dordrecht., 40(6), 1899-1906.
https://doi.org/10.1007/s10695-014-9977-9

Gačić Z, Bajić A, Milošević M, Nikčević M, Mićković B, Hegediš A, Gacic L, Damjanović I. Spectral sensitivity of the electroretinogram b-wave in dark-adapted Prussian carp (Carassius gibelio Bloch, 1782). in Fish Physiology and Biochemistry. 2014;40(6):1899-1906.
doi:10.1007/s10695-014-9977-9 .

Gačić, Zoran, Bajić, Aleksandar, Milošević, Milena, Nikčević, Miroslav, Mićković, Branislav, Hegediš, Aleksandar, Gacic, L., Damjanović, Ilija, "Spectral sensitivity of the electroretinogram b-wave in dark-adapted Prussian carp (Carassius gibelio Bloch, 1782)" in Fish Physiology and Biochemistry, 40, no. 6 (2014):1899-1906,
https://doi.org/10.1007/s10695-014-9977-9 . .

TY  - JOUR
AU  - Milošević, Milena
AU  - Višnjić Jeftić, Željka
AU  - Damjanović, Ilija
AU  - Nikčević, Miroslav
AU  - Andjus, Pavle R.
AU  - Gačić, Zoran
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/341
AB  - Photoreceptor content of fish retinas could be accessed by comparative electroretinographic (ERG) studies using flickering light stimuli that could separate rod-mediated vision where critical flicker frequency (CFE, frequency when the eye loses its ability to resolve individual light pulses) is usually less than 15 Hz from cone-mediated vision. Four fish species inhabiting different photic environments (small-spotted dogfish shark - Scyliorhinus canicula, eel - Anguilla anguilla, painted comber - Serranus scriba, Prussian carp - Carassius gibelio) were investigated. Dogfish shark b-wave amplitudes significantly decreased at low frequency of stimulation and CFF was reached at 3.2 Hz. A similar effect on the b-wave amplitude was observed in the eel, but CFF occurred at around 20 Hz. Conversely, b-waves of painted comber and Prussian carp remained unaltered under intermittent low-frequency stimulation, and CFFs were around 25 and 30 Hz, respectively. Additional support in accessing the receptor content of fish retinas was given by the characterization of the OFF-response (d-wave) after light adaptation. Monotonous time course of the b-wave dark adaptation indicated a rod dominated retina of the dogfish shark. Observed results indicate that the dogfish shark possesses preponderantly rod retina, that of the eel is rod-dominated, while Prussian carp and painted comber have cone-rich retinae.
PB  - General Physiol And Biophysics, Bratislava
T2  - General Physiology and Biophysics
T1  - Temporal analysis of electroretinographic responses in fishes with rod-dominated and mixed rod-cone retina
EP  - 282
IS  - 3
SP  - 276
VL  - 28
DO  - 10.4149/gpb_2009_03_276
ER  - 

@article{
author = "Milošević, Milena and Višnjić Jeftić, Željka and Damjanović, Ilija and Nikčević, Miroslav and Andjus, Pavle R. and Gačić, Zoran",
year = "2009",
abstract = "Photoreceptor content of fish retinas could be accessed by comparative electroretinographic (ERG) studies using flickering light stimuli that could separate rod-mediated vision where critical flicker frequency (CFE, frequency when the eye loses its ability to resolve individual light pulses) is usually less than 15 Hz from cone-mediated vision. Four fish species inhabiting different photic environments (small-spotted dogfish shark - Scyliorhinus canicula, eel - Anguilla anguilla, painted comber - Serranus scriba, Prussian carp - Carassius gibelio) were investigated. Dogfish shark b-wave amplitudes significantly decreased at low frequency of stimulation and CFF was reached at 3.2 Hz. A similar effect on the b-wave amplitude was observed in the eel, but CFF occurred at around 20 Hz. Conversely, b-waves of painted comber and Prussian carp remained unaltered under intermittent low-frequency stimulation, and CFFs were around 25 and 30 Hz, respectively. Additional support in accessing the receptor content of fish retinas was given by the characterization of the OFF-response (d-wave) after light adaptation. Monotonous time course of the b-wave dark adaptation indicated a rod dominated retina of the dogfish shark. Observed results indicate that the dogfish shark possesses preponderantly rod retina, that of the eel is rod-dominated, while Prussian carp and painted comber have cone-rich retinae.",
publisher = "General Physiol And Biophysics, Bratislava",
journal = "General Physiology and Biophysics",
title = "Temporal analysis of electroretinographic responses in fishes with rod-dominated and mixed rod-cone retina",
pages = "282-276",
number = "3",
volume = "28",
doi = "10.4149/gpb_2009_03_276"
}

Milošević, M., Višnjić Jeftić, Ž., Damjanović, I., Nikčević, M., Andjus, P. R.,& Gačić, Z.. (2009). Temporal analysis of electroretinographic responses in fishes with rod-dominated and mixed rod-cone retina. in General Physiology and Biophysics
General Physiol And Biophysics, Bratislava., 28(3), 276-282.
https://doi.org/10.4149/gpb_2009_03_276

Milošević M, Višnjić Jeftić Ž, Damjanović I, Nikčević M, Andjus PR, Gačić Z. Temporal analysis of electroretinographic responses in fishes with rod-dominated and mixed rod-cone retina. in General Physiology and Biophysics. 2009;28(3):276-282.
doi:10.4149/gpb_2009_03_276 .

Milošević, Milena, Višnjić Jeftić, Željka, Damjanović, Ilija, Nikčević, Miroslav, Andjus, Pavle R., Gačić, Zoran, "Temporal analysis of electroretinographic responses in fishes with rod-dominated and mixed rod-cone retina" in General Physiology and Biophysics, 28, no. 3 (2009):276-282,
https://doi.org/10.4149/gpb_2009_03_276 . .

TY  - JOUR
AU  - Gačić, Zoran
AU  - Bajić, Aleksandar
AU  - Milošević, Milena
AU  - Nikčević, Miroslav
AU  - Mićković, Branislav
AU  - Damjanović, Ilija
PY  - 2007
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/209
AB  - In spectral sensitivity studies, electroretinograms (ERG) were recorded from the in situ eyecup of immobilized perch (Perca fluviatilis) electrofished in the floodplain zone of the Danube River. It is shown that the ERG b-wave is a good indicator of spectral sensitivity, although it reflects the activity of photoreceptors indirectly. With a maximum around 542 mm, the scotopic spectral sensitivity of perch obtained using fitted amplitude-log intensity functions for threshold calculation and two models developed in our laboratory for computer-assisted fitting of spectral sensitivity curves did not differ from the sensitivity determined using the microspectrophotometric and isolated pigment methods (maximum around 541 nm).
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Spectral sensitivity of the perch (Perca fluviatilis) from the danube
EP  - 340
IS  - 4
SP  - 335
VL  - 59
DO  - 10.2298/ABS0704335G
ER  - 

@article{
author = "Gačić, Zoran and Bajić, Aleksandar and Milošević, Milena and Nikčević, Miroslav and Mićković, Branislav and Damjanović, Ilija",
year = "2007",
abstract = "In spectral sensitivity studies, electroretinograms (ERG) were recorded from the in situ eyecup of immobilized perch (Perca fluviatilis) electrofished in the floodplain zone of the Danube River. It is shown that the ERG b-wave is a good indicator of spectral sensitivity, although it reflects the activity of photoreceptors indirectly. With a maximum around 542 mm, the scotopic spectral sensitivity of perch obtained using fitted amplitude-log intensity functions for threshold calculation and two models developed in our laboratory for computer-assisted fitting of spectral sensitivity curves did not differ from the sensitivity determined using the microspectrophotometric and isolated pigment methods (maximum around 541 nm).",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Spectral sensitivity of the perch (Perca fluviatilis) from the danube",
pages = "340-335",
number = "4",
volume = "59",
doi = "10.2298/ABS0704335G"
}

Gačić, Z., Bajić, A., Milošević, M., Nikčević, M., Mićković, B.,& Damjanović, I.. (2007). Spectral sensitivity of the perch (Perca fluviatilis) from the danube. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 59(4), 335-340.
https://doi.org/10.2298/ABS0704335G

Gačić Z, Bajić A, Milošević M, Nikčević M, Mićković B, Damjanović I. Spectral sensitivity of the perch (Perca fluviatilis) from the danube. in Archives of Biological Sciences. 2007;59(4):335-340.
doi:10.2298/ABS0704335G .

Gačić, Zoran, Bajić, Aleksandar, Milošević, Milena, Nikčević, Miroslav, Mićković, Branislav, Damjanović, Ilija, "Spectral sensitivity of the perch (Perca fluviatilis) from the danube" in Archives of Biological Sciences, 59, no. 4 (2007):335-340,
https://doi.org/10.2298/ABS0704335G . .

TY  - JOUR
AU  - Gačić, Zoran
AU  - Damjanović, Ilija
AU  - Konjević, D
AU  - Bajić, Aleksandar
AU  - Milošević, Milena
AU  - Andjus, PR
AU  - Mićković, Branislav
AU  - Andjus, RK
PY  - 2005
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/141
AB  - Scotopic electroretinogram of dogfish shark (Scylliorhinus canicula) and eel (Anguilla anguilla) is characterized by a negative off-response, changing in sign under photopic condition. It increased under the effect of increased background illumination, but its amplitude never exceeded that of the b-wave. On the other hand, dark-adapted electroretinograms of two perch-like species, perch (Perca fluviatilis) and painted comber (Serranus scriba), exhibited a positive off-wave, exceeding the b-wave amplitude under bright photopic conditions.
PB  - New York Acad Sciences, New York
T2  - Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
T1  - Influence of photic environment on the form of the fish electroretinographic off-response
EP  - 440
SP  - 437
VL  - 1048
DO  - 10.1196/annals.1342.058
ER  - 

@article{
author = "Gačić, Zoran and Damjanović, Ilija and Konjević, D and Bajić, Aleksandar and Milošević, Milena and Andjus, PR and Mićković, Branislav and Andjus, RK",
year = "2005",
abstract = "Scotopic electroretinogram of dogfish shark (Scylliorhinus canicula) and eel (Anguilla anguilla) is characterized by a negative off-response, changing in sign under photopic condition. It increased under the effect of increased background illumination, but its amplitude never exceeded that of the b-wave. On the other hand, dark-adapted electroretinograms of two perch-like species, perch (Perca fluviatilis) and painted comber (Serranus scriba), exhibited a positive off-wave, exceeding the b-wave amplitude under bright photopic conditions.",
publisher = "New York Acad Sciences, New York",
journal = "Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus",
title = "Influence of photic environment on the form of the fish electroretinographic off-response",
pages = "440-437",
volume = "1048",
doi = "10.1196/annals.1342.058"
}

Gačić, Z., Damjanović, I., Konjević, D., Bajić, A., Milošević, M., Andjus, P., Mićković, B.,& Andjus, R.. (2005). Influence of photic environment on the form of the fish electroretinographic off-response. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
New York Acad Sciences, New York., 1048, 437-440.
https://doi.org/10.1196/annals.1342.058

Gačić Z, Damjanović I, Konjević D, Bajić A, Milošević M, Andjus P, Mićković B, Andjus R. Influence of photic environment on the form of the fish electroretinographic off-response. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus. 2005;1048:437-440.
doi:10.1196/annals.1342.058 .

Gačić, Zoran, Damjanović, Ilija, Konjević, D, Bajić, Aleksandar, Milošević, Milena, Andjus, PR, Mićković, Branislav, Andjus, RK, "Influence of photic environment on the form of the fish electroretinographic off-response" in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus, 1048 (2005):437-440,
https://doi.org/10.1196/annals.1342.058 . .